Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016750 | XP_003602822.2 | 96.894 | 966 | 29 | 1 | 268 | 1233 | 13 | 977 | 0.0 | 1939 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016750 | sp|Q9M9E8|FB92_ARATH | 59.939 | 976 | 337 | 13 | 268 | 1229 | 8 | 943 | 0.0 | 1184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016750 | G7J3X8 | 96.894 | 966 | 29 | 1 | 268 | 1233 | 13 | 977 | 0.0 | 1939 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG016750 | TR | Jumonji |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG016750 | MsaG016645 | 0.824683 | 1.252421e-53 | 3.123194e-51 |
| MsaG016750 | MsaG018628 | 0.807052 | 1.036037e-49 | 1.648197e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016750 | MtrunA17_Chr3g0132281 | 96.894 | 966 | 29 | 1 | 268 | 1233 | 13 | 977 | 0.0 | 1939 |
| MsaG016750 | MtrunA17_Chr3g0132271 | 98.319 | 238 | 4 | 0 | 1 | 238 | 1 | 238 | 1.02e-163 | 486 |
| MsaG016750 | MtrunA17_Chr2g0302191 | 97.706 | 218 | 5 | 0 | 1 | 218 | 1 | 218 | 9.37e-149 | 446 |
| MsaG016750 | MtrunA17_Chr7g0268031 | 38.814 | 371 | 214 | 8 | 267 | 634 | 88 | 448 | 2.46e-74 | 256 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG016750 | AT1G78280.1 | 59.939 | 976 | 337 | 13 | 268 | 1229 | 8 | 943 | 0.0 | 1184 |
| MsaG016750 | AT5G06550.1 | 37.875 | 367 | 215 | 8 | 267 | 631 | 73 | 428 | 2.25e-73 | 254 |
| MsaG016750 | AT5G50410.1 | 50.216 | 231 | 106 | 5 | 13 | 238 | 33 | 259 | 2.37e-67 | 228 |
| MsaG016750 | AT5G63080.1 | 26.690 | 281 | 164 | 11 | 388 | 634 | 7 | 279 | 1.56e-18 | 90.9 |
Find 295 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTTCTTTGTTAAGATTC+TGG | 0.145954 | 3:-101668697 | None:intergenic |
| GCATGGAATACTCTTCTTTC+TGG | 0.157901 | 3:+101664690 | MsaT016750.1:CDS |
| TGACTTGATACCAATTTATT+TGG | 0.164384 | 3:+101671122 | MsaT016750.1:CDS |
| TGATTTCAGTGATCTTTCTA+TGG | 0.200906 | 3:+101669473 | MsaT016750.1:CDS |
| GTCTACATCTTTCAGAGTTT+TGG | 0.213866 | 3:-101666429 | None:intergenic |
| GGACTGGAAGCATCGCTTTA+TGG | 0.213881 | 3:+101667934 | MsaT016750.1:CDS |
| CATCCAGAAGCTATAATTTA+TGG | 0.214640 | 3:+101666483 | MsaT016750.1:CDS |
| GATATAAATGAATTATTTAA+AGG | 0.220719 | 3:-101668579 | None:intergenic |
| CGAATTTGTATGTTTGGATA+TGG | 0.225637 | 3:+101666265 | MsaT016750.1:CDS |
| CATAACTGTTCTTGGATATC+AGG | 0.229022 | 3:-101656043 | None:intergenic |
| CTCTTCTTTCTGGCCGTAAA+AGG | 0.234128 | 3:+101664700 | MsaT016750.1:CDS |
| TCCTATAGGTGGCTTATAAT+TGG | 0.239011 | 3:+101664618 | MsaT016750.1:CDS |
| CAAAGATAGTGATTTGTTCT+TGG | 0.243429 | 3:+101658933 | MsaT016750.1:CDS |
| CACCCTCAGGTCATGCTAAA+TGG | 0.250190 | 3:+101664187 | MsaT016750.1:intron |
| CATATTAGATCTCTCATATC+TGG | 0.253644 | 3:-101666650 | None:intergenic |
| CAAGAACAAATCACTATCTT+TGG | 0.263525 | 3:-101658932 | None:intergenic |
| ACGTGGATGGTTTCCCGTTT+GGG | 0.269531 | 3:+101668212 | MsaT016750.1:CDS |
| CTCCGCCATGGTTACTTCCT+TGG | 0.275667 | 3:-101655552 | None:intergenic |
| TTGGATACACACTGACATTA+TGG | 0.277456 | 3:+101669317 | MsaT016750.1:CDS |
| CTAAACACATCCAAATAAAT+TGG | 0.279520 | 3:-101671132 | None:intergenic |
| TAACTTCGAATTTGTATGTT+TGG | 0.284606 | 3:+101666259 | MsaT016750.1:CDS |
| TTTGATATGTTGCCAGAAAA+AGG | 0.286273 | 3:-101671201 | None:intergenic |
| AACAAACTTGGCCTCCTTCC+TGG | 0.289607 | 3:+101668518 | MsaT016750.1:CDS |
| CATGGTTACTTCCTTGGGTT+CGG | 0.290212 | 3:-101655546 | None:intergenic |
| TTGTTTGAGTATAGGAATAC+TGG | 0.294582 | 3:+101668250 | MsaT016750.1:CDS |
| TTTCCATAAATTATAGCTTC+TGG | 0.297974 | 3:-101666486 | None:intergenic |
| GCAATCAGTTTGCTAGGAAT+TGG | 0.306034 | 3:-101669108 | None:intergenic |
| CAGATATGAGAGATCTAATA+TGG | 0.307979 | 3:+101666651 | MsaT016750.1:CDS |
| TGGTTTCCCGTTTGGGGTAT+GGG | 0.311759 | 3:+101668219 | MsaT016750.1:CDS |
| GACATAATCCTTGAACTTCA+TGG | 0.326012 | 3:-101664317 | None:intergenic |
| CTCGCGTCGCTTGCGTTAGC+AGG | 0.327432 | 3:+101662286 | MsaT016750.1:CDS |
| CATCGCTTAATTATCAAATA+TGG | 0.329184 | 3:-101668322 | None:intergenic |
| TAGGAAGCTTCGAGAATATT+GGG | 0.331747 | 3:-101659113 | None:intergenic |
| ATGGTTTCCCGTTTGGGGTA+TGG | 0.336226 | 3:+101668218 | MsaT016750.1:CDS |
| ATGGTTACTTCCTTGGGTTC+GGG | 0.336939 | 3:-101655545 | None:intergenic |
| AACGTGGATGGTTTCCCGTT+TGG | 0.339996 | 3:+101668211 | MsaT016750.1:CDS |
| AGTGGGGCTGGGCGTTTCAT+TGG | 0.342287 | 3:-101657447 | None:intergenic |
| GCCTGGAGCATAACTGTTCT+TGG | 0.343445 | 3:-101656051 | None:intergenic |
| ATAGGAAGCTTCGAGAATAT+TGG | 0.346895 | 3:-101659114 | None:intergenic |
| CATCCATAGTGGTTCTTCAT+TGG | 0.346934 | 3:-101662919 | None:intergenic |
| ACTCTGGAGTCCTACCCTTC+TGG | 0.355704 | 3:+101659905 | MsaT016750.1:CDS |
| GGATGAGGTAGTTAAGTCTA+AGG | 0.356450 | 3:+101655994 | MsaT016750.1:CDS |
| TGGCACTCTTTGTACTTCTC+TGG | 0.357779 | 3:-101663122 | None:intergenic |
| ATCCCAACTTAAATTGGTAT+AGG | 0.361855 | 3:-101668134 | None:intergenic |
| GAGAATGGTTGTCCAAGCTT+TGG | 0.365534 | 3:+101666618 | MsaT016750.1:CDS |
| GATCAGCTGTTACAAAACTA+TGG | 0.367650 | 3:+101664250 | MsaT016750.1:CDS |
| GAAAGGCTTCCTGTTGGTTC+GGG | 0.369837 | 3:+101666861 | MsaT016750.1:CDS |
| TTTCGTCGAAAATATATAAT+GGG | 0.370958 | 3:-101664369 | None:intergenic |
| CGAAAGGCTTCCTGTTGGTT+CGG | 0.372369 | 3:+101666860 | MsaT016750.1:CDS |
| TCCGCCATGGTTACTTCCTT+GGG | 0.373829 | 3:-101655551 | None:intergenic |
| TCGCCTTACTGGTTTGTGAT+AGG | 0.377750 | 3:-101659132 | None:intergenic |
| CATGGCCTGCCAGGCAGAAA+TGG | 0.378327 | 3:+101664221 | MsaT016750.1:CDS |
| AATGTAAAGGCTGCTGTCTA+TGG | 0.380107 | 3:-101663142 | None:intergenic |
| ACTCTTGGAAGATTGATTAC+AGG | 0.380471 | 3:-101658907 | None:intergenic |
| ATGAATACATGAAACTAGTT+AGG | 0.380734 | 3:+101659844 | MsaT016750.1:CDS |
| AGCATACTTGGAAGTGGGGC+TGG | 0.381098 | 3:-101657459 | None:intergenic |
| GTTTCTAACCAAGCATAAAC+TGG | 0.383158 | 3:-101655962 | None:intergenic |
| ATTTATTTGGATGTGTTTAG+AGG | 0.384148 | 3:+101671135 | MsaT016750.1:CDS |
| CGGTTTCAGTTTACTTGAGT+TGG | 0.385352 | 3:+101659077 | MsaT016750.1:intron |
| AATGAAATAGTGACCTGATT+GGG | 0.386123 | 3:-101655686 | None:intergenic |
| ATCAAACAATGCAGCAGAAT+GGG | 0.387429 | 3:+101668665 | MsaT016750.1:CDS |
| TCACTATCTTTGGGAACTCT+TGG | 0.388652 | 3:-101658922 | None:intergenic |
| TAGCCGGTAACTCAAGTATA+TGG | 0.392078 | 3:+101668299 | MsaT016750.1:CDS |
| TGGCCATATACTTGAGTTAC+CGG | 0.392860 | 3:-101668302 | None:intergenic |
| TTGGTTAGAAACGTTAAATA+AGG | 0.396252 | 3:+101655973 | MsaT016750.1:CDS |
| TGGATGACTCTATGCCTAAA+AGG | 0.397240 | 3:+101662936 | MsaT016750.1:CDS |
| TTTGAATGGAGATGAGTCTA+TGG | 0.398747 | 3:-101655649 | None:intergenic |
| CCTCCTGGAAAAGTGCCTTT+AGG | 0.405897 | 3:+101665878 | MsaT016750.1:CDS |
| TGCCTAAAAGGAGGAGCATC+TGG | 0.408109 | 3:+101662948 | MsaT016750.1:CDS |
| GGGCAACAGCATTGGTCAAC+GGG | 0.409974 | 3:+101666589 | MsaT016750.1:CDS |
| GGTAGTTGTGTACATTCAAT+TGG | 0.411189 | 3:-101666143 | None:intergenic |
| GTATAGGAATACTGGGATTC+CGG | 0.412569 | 3:+101668258 | MsaT016750.1:CDS |
| ACAAACTTGGCCTCCTTCCT+GGG | 0.419271 | 3:+101668519 | MsaT016750.1:CDS |
| AAGAACAAATCACTATCTTT+GGG | 0.419383 | 3:-101658931 | None:intergenic |
| GTTGTCCATTTCTGCCTGGC+AGG | 0.425101 | 3:-101664226 | None:intergenic |
| GAAACATATTCCTAGTGTTA+TGG | 0.425446 | 3:+101668081 | MsaT016750.1:CDS |
| CAGGCATTCTAACCACTTGC+CGG | 0.427854 | 3:-101666793 | None:intergenic |
| ATATTGAACGTGAATTAAAC+TGG | 0.429371 | 3:+101668604 | MsaT016750.1:CDS |
| AATCCTATACCAATTTAAGT+TGG | 0.431151 | 3:+101668131 | MsaT016750.1:CDS |
| TTGCATGACGATAATGTCTT+GGG | 0.433068 | 3:+101673542 | MsaT016750.1:CDS |
| TGGCGAATACATGGCCTGCC+AGG | 0.433299 | 3:+101664212 | MsaT016750.1:CDS |
| GATTTCAGTGATCTTTCTAT+GGG | 0.433748 | 3:+101669474 | MsaT016750.1:CDS |
| ACATGCCCCTAGCTTATTGA+AGG | 0.436977 | 3:+101664530 | MsaT016750.1:CDS |
| ACAGAGGGTGGTCTAAAGTT+TGG | 0.438033 | 3:+101671234 | MsaT016750.1:CDS |
| GCAAGCAATTGTTTGAGTAT+AGG | 0.439732 | 3:+101668242 | MsaT016750.1:CDS |
| ATCCTATACCAATTTAAGTT+GGG | 0.441548 | 3:+101668132 | MsaT016750.1:CDS |
| ATCAGTTGTCCATTTCTGCC+TGG | 0.443048 | 3:-101664230 | None:intergenic |
| GAAGCATCGCTTTATGGTTT+AGG | 0.443732 | 3:+101667940 | MsaT016750.1:CDS |
| CACAGAGATCTGGTGCCTCT+TGG | 0.445712 | 3:+101664643 | MsaT016750.1:CDS |
| TACTTCCTTGGGTTCGGGGA+AGG | 0.446159 | 3:-101655540 | None:intergenic |
| AAAGATCTCCATGAAGTTCA+AGG | 0.447205 | 3:+101664309 | MsaT016750.1:CDS |
| TGCTTGCCCCATACCCCAAA+CGG | 0.447802 | 3:-101668226 | None:intergenic |
| TGTAAATAGGTTTATCTAGT+GGG | 0.450022 | 3:+101667880 | MsaT016750.1:intron |
| CACAAGAGCAAGAACAAGAA+TGG | 0.451021 | 3:+101655489 | MsaT016750.1:CDS |
| ATGAGTCTATGGCAAAGATC+GGG | 0.452435 | 3:-101655638 | None:intergenic |
| GCACCTGGTTATCGTCATAA+AGG | 0.453039 | 3:+101666287 | MsaT016750.1:CDS |
| TTCTTCAATACAAAGGCTCT+TGG | 0.456556 | 3:+101662973 | MsaT016750.1:CDS |
| TATCCAATGAAGAACCACTA+TGG | 0.457458 | 3:+101662916 | MsaT016750.1:CDS |
| TTGCATGAGACTCCCCAATC+AGG | 0.459269 | 3:+101655673 | MsaT016750.1:CDS |
| TTTGCATGACGATAATGTCT+TGG | 0.462274 | 3:+101673541 | MsaT016750.1:CDS |
| CTGAAATCAAGGAGGTAACT+AGG | 0.465174 | 3:-101669459 | None:intergenic |
| GGTTTCAACGAACTGATCAA+TGG | 0.465541 | 3:+101655936 | MsaT016750.1:CDS |
| GCACTTCTAAACCCTGCTCT+TGG | 0.465947 | 3:+101669298 | MsaT016750.1:CDS |
| TTCCATGCACTTGTAAGAGC+TGG | 0.466147 | 3:-101664675 | None:intergenic |
| TTCCTAGTGTTATGGCCAGT+GGG | 0.468965 | 3:+101668089 | MsaT016750.1:CDS |
| TAGAAAGATCACTGAAATCA+AGG | 0.469489 | 3:-101669470 | None:intergenic |
| GTCAGTGTGTATCCAAGAGC+AGG | 0.469541 | 3:-101669310 | None:intergenic |
| AAGTATACCACTTGGTCAAA+CGG | 0.469956 | 3:-101668734 | None:intergenic |
| CAGATAAACGACCATCCTAT+AGG | 0.470762 | 3:+101664604 | MsaT016750.1:CDS |
| CCTTTCGTCATCTGTGGGAG+GGG | 0.475975 | 3:-101666844 | None:intergenic |
| GGCATGGATTGTATACCCTC+TGG | 0.478814 | 3:-101655611 | None:intergenic |
| TTGAAGCCAACTCCCCTCTC+AGG | 0.479773 | 3:+101668059 | MsaT016750.1:CDS |
| TCTAGAACCTGCAGTGATGA+TGG | 0.481252 | 3:-101665950 | None:intergenic |
| AATGGCTTCCAGTTTATGCT+TGG | 0.486059 | 3:+101655954 | MsaT016750.1:CDS |
| TCCAATTATAAGCCACCTAT+AGG | 0.486415 | 3:-101664619 | None:intergenic |
| ATAATTGGACCACAGAGATC+TGG | 0.489173 | 3:+101664633 | MsaT016750.1:CDS |
| GAATGAAATAGTGACCTGAT+TGG | 0.489960 | 3:-101655687 | None:intergenic |
| GCCTTTCGTCATCTGTGGGA+GGG | 0.490405 | 3:-101666845 | None:intergenic |
| ACAAGAGCAAGAACAAGAAT+GGG | 0.495512 | 3:+101655490 | MsaT016750.1:CDS |
| TTTAATGACAACTCTTTGCC+AGG | 0.495952 | 3:-101657408 | None:intergenic |
| GAATTATTTAAAGGTGGATG+TGG | 0.496530 | 3:-101668570 | None:intergenic |
| AATCAAACAATGCAGCAGAA+TGG | 0.499094 | 3:+101668664 | MsaT016750.1:CDS |
| CAATTTATGTTCCGAGTGGA+TGG | 0.499365 | 3:+101666174 | MsaT016750.1:CDS |
| GGTTTCCCGTTTGGGGTATG+GGG | 0.499368 | 3:+101668220 | MsaT016750.1:CDS |
| ACATGCACCGTCACACCTAA+AGG | 0.501322 | 3:-101665893 | None:intergenic |
| TGTTGCTGCATTTGCATCAC+CGG | 0.502110 | 3:-101655468 | None:intergenic |
| AGGTGGGCATTATATCCTCC+TGG | 0.509762 | 3:+101665863 | MsaT016750.1:intron |
| GCTTGCCCCATACCCCAAAC+GGG | 0.510621 | 3:-101668225 | None:intergenic |
| ATGTTTCCTGAGAGGGGAGT+TGG | 0.512138 | 3:-101668065 | None:intergenic |
| AGAAGTTGAGATGACAGTGT+GGG | 0.513193 | 3:+101673613 | MsaT016750.1:CDS |
| GCATACTTGGAAGTGGGGCT+GGG | 0.513217 | 3:-101657458 | None:intergenic |
| TGGAGCTCGGGCAACAGCAT+TGG | 0.513686 | 3:+101666581 | MsaT016750.1:CDS |
| TATACCAATTTAAGTTGGGA+TGG | 0.514509 | 3:+101668136 | MsaT016750.1:CDS |
| TAAATGGACTGGCGAATACA+TGG | 0.516680 | 3:+101664203 | MsaT016750.1:CDS |
| ATTGAGCAGAGGATTTCATC+GGG | 0.517061 | 3:-101662234 | None:intergenic |
| AATTTAAGTTGGGATGGAAA+AGG | 0.517463 | 3:+101668142 | MsaT016750.1:CDS |
| GACAGATCAGTTAGTTTAGC+CGG | 0.517994 | 3:+101668283 | MsaT016750.1:CDS |
| CAGTAATCCTTCAATAAGCT+AGG | 0.518812 | 3:-101664537 | None:intergenic |
| AACTGAGATCATCAGAGTCA+TGG | 0.520852 | 3:+101673588 | MsaT016750.1:CDS |
| AATTGAGCAGAGGATTTCAT+CGG | 0.521661 | 3:-101662235 | None:intergenic |
| TCCAAGAACAGTTATGCTCC+AGG | 0.522275 | 3:+101656050 | MsaT016750.1:CDS |
| AATAAATGACATCTCAATGA+AGG | 0.522634 | 3:+101663381 | MsaT016750.1:CDS |
| CAAGTATGACATGAAGAAAC+CGG | 0.525208 | 3:+101663414 | MsaT016750.1:CDS |
| CGTGGTATTATCGTCAACTC+TGG | 0.527061 | 3:+101659889 | MsaT016750.1:CDS |
| TAAGTTACTCTGATTTGTCA+AGG | 0.527231 | 3:+101666393 | MsaT016750.1:CDS |
| TTGATAATTAAGCGATGTGA+GGG | 0.528989 | 3:+101668328 | MsaT016750.1:CDS |
| TAGGAATATGTTTCCTGAGA+GGG | 0.530545 | 3:-101668072 | None:intergenic |
| GATGAGTCTATGGCAAAGAT+CGG | 0.534286 | 3:-101655639 | None:intergenic |
| CGGGCAACAGCATTGGTCAA+CGG | 0.534919 | 3:+101666588 | MsaT016750.1:CDS |
| AGGCATAGAGTCATCCATAG+TGG | 0.536115 | 3:-101662930 | None:intergenic |
| ATAAATGAATTATTTAAAGG+TGG | 0.536575 | 3:-101668576 | None:intergenic |
| TATGGATGATAATATTTACA+TGG | 0.536763 | 3:+101669335 | MsaT016750.1:CDS |
| ATTGCTCTTCAAAATGACAC+TGG | 0.537117 | 3:-101668170 | None:intergenic |
| GAATAAAGCATACTTGGAAG+TGG | 0.537458 | 3:-101657465 | None:intergenic |
| AATATTGAAAAGAAGACAGA+AGG | 0.538817 | 3:+101656120 | MsaT016750.1:CDS |
| CAAATGATAACGAGTATGCC+TGG | 0.539445 | 3:-101656068 | None:intergenic |
| GCAAAGATCGGGGCGAGGCA+TGG | 0.540391 | 3:-101655627 | None:intergenic |
| AGTAATCCTTCAATAAGCTA+GGG | 0.541163 | 3:-101664536 | None:intergenic |
| TGTTTGAGTATAGGAATACT+GGG | 0.542687 | 3:+101668251 | MsaT016750.1:CDS |
| CAAGGATTATGTCTCATACA+TGG | 0.543266 | 3:+101664327 | MsaT016750.1:CDS |
| GTTCGTTGAAACCGCGCATG+AGG | 0.544794 | 3:-101655926 | None:intergenic |
| GTGCTCCTTCCCCGAACCCA+AGG | 0.547004 | 3:+101655535 | MsaT016750.1:CDS |
| GCATGCATTGCACTAAATGC+CGG | 0.547137 | 3:+101666774 | MsaT016750.1:CDS |
| CTGTAAATAGGTTTATCTAG+TGG | 0.547357 | 3:+101667879 | MsaT016750.1:intron |
| TAAGCTGCTAAATATAAATG+AGG | 0.548356 | 3:+101669164 | MsaT016750.1:CDS |
| GGAACATAAATTGTCTCTCC+AGG | 0.550858 | 3:-101666164 | None:intergenic |
| AGGAAGCCTTTCGTCATCTG+TGG | 0.551264 | 3:-101666850 | None:intergenic |
| ATAACTGTTCTTGGATATCA+GGG | 0.551271 | 3:-101656042 | None:intergenic |
| GATCGCAGAATAGACGCTCT+TGG | 0.552054 | 3:+101662195 | MsaT016750.1:CDS |
| ATCCAGCTCTTACAAGTGCA+TGG | 0.557351 | 3:+101664673 | MsaT016750.1:CDS |
| AATAAAGCATACTTGGAAGT+GGG | 0.558010 | 3:-101657464 | None:intergenic |
| ACCACGTTGGAGTGAAATCC+TGG | 0.558962 | 3:+101669433 | MsaT016750.1:CDS |
| GCGACGCGAGCAGCATCTTG+AGG | 0.560780 | 3:-101662273 | None:intergenic |
| GGATTGCTACCCGAACCAAC+AGG | 0.563415 | 3:-101666870 | None:intergenic |
| GTGGGAAGATACAACAAACT+TGG | 0.564350 | 3:+101668506 | MsaT016750.1:CDS |
| GAGGTAGAGCAAATCGATGA+TGG | 0.564450 | 3:-101669360 | None:intergenic |
| TTGCATTTAAAATTTCTCAA+AGG | 0.564534 | 3:+101664278 | MsaT016750.1:CDS |
| CGTGGATGGTTTCCCGTTTG+GGG | 0.564553 | 3:+101668213 | MsaT016750.1:CDS |
| GATAATACCACGTGCAGTTG+AGG | 0.566216 | 3:-101659878 | None:intergenic |
| AGAAACGTTAAATAAGGATG+AGG | 0.566372 | 3:+101655979 | MsaT016750.1:CDS |
| TTGTTACCATCAAAATGTAA+AGG | 0.567945 | 3:-101663155 | None:intergenic |
| AGAAGCTATAATTTATGGAA+AGG | 0.568532 | 3:+101666488 | MsaT016750.1:CDS |
| TGAGACTCCATCATCACTGC+AGG | 0.568853 | 3:+101665943 | MsaT016750.1:CDS |
| CCATGGCGGAGGTTGAAGCT+TGG | 0.569200 | 3:+101655564 | MsaT016750.1:CDS |
| CATAAAGGAGTCTGCCGTGT+GGG | 0.569358 | 3:+101666302 | MsaT016750.1:CDS |
| CCAGAATCTTAACAAAGAAG+AGG | 0.570253 | 3:+101668697 | MsaT016750.1:CDS |
| AGCCTTTCGTCATCTGTGGG+AGG | 0.570411 | 3:-101666846 | None:intergenic |
| TGGTTACTTCCTTGGGTTCG+GGG | 0.574233 | 3:-101655544 | None:intergenic |
| ACCCCACTGGCCATAACACT+AGG | 0.575861 | 3:-101668091 | None:intergenic |
| TCAGGTCATGCTAAATGGAC+TGG | 0.577021 | 3:+101664192 | MsaT016750.1:intron |
| AGCATCTTCTGTATTCCCAC+TGG | 0.577196 | 3:-101669382 | None:intergenic |
| GCATGGATTGTATACCCTCT+GGG | 0.579035 | 3:-101655610 | None:intergenic |
| ACTGAGATCATCAGAGTCAT+GGG | 0.579147 | 3:+101673589 | MsaT016750.1:CDS |
| AGCTATAATTTATGGAAAGG+TGG | 0.579530 | 3:+101666491 | MsaT016750.1:CDS |
| ATTCCTAGTGTTATGGCCAG+TGG | 0.580711 | 3:+101668088 | MsaT016750.1:CDS |
| ATCAAAGTACGAGTCAACAG+AGG | 0.583079 | 3:+101671218 | MsaT016750.1:CDS |
| GAAGTTGAGATGACAGTGTG+GGG | 0.583600 | 3:+101673614 | MsaT016750.1:CDS |
| TCTCAAGCCGTTTGACCAAG+TGG | 0.584548 | 3:+101668727 | MsaT016750.1:CDS |
| CTTTCGTCATCTGTGGGAGG+GGG | 0.587951 | 3:-101666843 | None:intergenic |
| TCCTAGTGTTATGGCCAGTG+GGG | 0.588471 | 3:+101668090 | MsaT016750.1:CDS |
| GAAGCAATCATGTCGAACTC+CGG | 0.588976 | 3:+101655449 | None:intergenic |
| GTCATCCTTGCTAACCAGAA+GGG | 0.590376 | 3:-101659919 | None:intergenic |
| GTCAACGGGAACTAAGAGAA+TGG | 0.591171 | 3:+101666603 | MsaT016750.1:CDS |
| AGCAGCTTAGATAAATCAGA+TGG | 0.592390 | 3:-101669150 | None:intergenic |
| TTTGATAATTAAGCGATGTG+AGG | 0.594501 | 3:+101668327 | MsaT016750.1:CDS |
| TCGATTTGCTCTACCTCCAG+TGG | 0.595230 | 3:+101669366 | MsaT016750.1:CDS |
| ACAGAGAAATGCAACGTGGA+TGG | 0.595885 | 3:+101668199 | MsaT016750.1:CDS |
| GTAAATAGGTTTATCTAGTG+GGG | 0.600129 | 3:+101667881 | MsaT016750.1:intron |
| ATCTTCTGTATTCCCACTGG+AGG | 0.600340 | 3:-101669379 | None:intergenic |
| TATGAGAGATCTAATATGGA+AGG | 0.601472 | 3:+101666655 | MsaT016750.1:CDS |
| ATGAAATAGTGACCTGATTG+GGG | 0.601718 | 3:-101655685 | None:intergenic |
| CAAGGAAGTAACCATGGCGG+AGG | 0.601867 | 3:+101655553 | MsaT016750.1:CDS |
| ATATTGAAAAGAAGACAGAA+GGG | 0.603047 | 3:+101656121 | MsaT016750.1:CDS |
| GGGCAACGATGGCTCAAGCA+TGG | 0.604205 | 3:+101655510 | MsaT016750.1:CDS |
| TGACTTCAGAATTTCTCAAG+TGG | 0.604991 | 3:+101669276 | MsaT016750.1:intron |
| AATCTTAACAAAGAAGAGGA+AGG | 0.605956 | 3:+101668701 | MsaT016750.1:CDS |
| GAAAAGAAGACAGAAGGGGA+AGG | 0.606257 | 3:+101656126 | MsaT016750.1:CDS |
| AATACAGAAGATGCTGCCGA+GGG | 0.606356 | 3:+101669390 | MsaT016750.1:CDS |
| GTATGTTTGGATATGGCACC+TGG | 0.607998 | 3:+101666272 | MsaT016750.1:CDS |
| ATTATAAGCCACCTATAGGA+TGG | 0.608178 | 3:-101664615 | None:intergenic |
| CCAAGCTTCAACCTCCGCCA+TGG | 0.610092 | 3:-101655564 | None:intergenic |
| CCCCTCCCACAGATGACGAA+AGG | 0.610507 | 3:+101666844 | MsaT016750.1:CDS |
| TTGCACTAAATGCCGGCAAG+TGG | 0.611811 | 3:+101666781 | MsaT016750.1:CDS |
| GTGCATGTTAATGAAGAAGA+TGG | 0.611852 | 3:+101665908 | MsaT016750.1:CDS |
| AGAACAAGAATGGGCAACGA+TGG | 0.612385 | 3:+101655499 | MsaT016750.1:CDS |
| AGCTCCACTCAAAATTGTAG+TGG | 0.612842 | 3:-101666565 | None:intergenic |
| CTATGGCAAAGATCGGGGCG+AGG | 0.613426 | 3:-101655632 | None:intergenic |
| GGAAGCCTTTCGTCATCTGT+GGG | 0.613871 | 3:-101666849 | None:intergenic |
| CAGAAGAGACAGACTATCGT+GGG | 0.616345 | 3:+101668488 | MsaT016750.1:intron |
| AAACTAACTGATCTGTCCGC+CGG | 0.617704 | 3:-101668277 | None:intergenic |
| CAAACTTGGCCTCCTTCCTG+GGG | 0.618358 | 3:+101668520 | MsaT016750.1:CDS |
| AACCTCTCATCTCTCCCAGA+GGG | 0.618396 | 3:+101655596 | MsaT016750.1:CDS |
| TGTTTAGAAAGAGAAATAAG+TGG | 0.619138 | 3:+101666458 | MsaT016750.1:CDS |
| TTGCTCTTCAAAATGACACT+GGG | 0.620536 | 3:-101668169 | None:intergenic |
| GAATACAGAAGATGCTGCCG+AGG | 0.621862 | 3:+101669389 | MsaT016750.1:CDS |
| GGACAGGGTTTAGAGCAGCC+TGG | 0.626069 | 3:+101657390 | MsaT016750.1:intron |
| TACAGAAGATGCTGCCGAGG+GGG | 0.626315 | 3:+101669392 | MsaT016750.1:CDS |
| TTGTCTTCGTCAGCAAGAAG+AGG | 0.627512 | 3:-101666119 | None:intergenic |
| AGGAATATGTTTCCTGAGAG+GGG | 0.628568 | 3:-101668071 | None:intergenic |
| GTGGATGTGGTAAAAGATGA+AGG | 0.628693 | 3:-101668557 | None:intergenic |
| CGATTTGCTCTACCTCCAGT+GGG | 0.629855 | 3:+101669367 | MsaT016750.1:CDS |
| TATTGAAAAGAAGACAGAAG+GGG | 0.630711 | 3:+101656122 | MsaT016750.1:CDS |
| ATACAGTGCCACCATCCACT+CGG | 0.631096 | 3:-101666185 | None:intergenic |
| TCAAACAATGCAGCAGAATG+GGG | 0.631106 | 3:+101668666 | MsaT016750.1:CDS |
| GACGTTCGAGAATTGAGCAG+AGG | 0.634922 | 3:-101662245 | None:intergenic |
| GATGACGAAAGGCTTCCTGT+TGG | 0.636057 | 3:+101666855 | MsaT016750.1:CDS |
| TCCTTGCTAACCAGAAGGGT+AGG | 0.636900 | 3:-101659915 | None:intergenic |
| TTTATGTTCCGAGTGGATGG+TGG | 0.637501 | 3:+101666177 | MsaT016750.1:CDS |
| TTAAACTGGTCTGAGGTGAA+AGG | 0.638216 | 3:+101668618 | MsaT016750.1:CDS |
| TTCAAGATAAACAACCCCAC+TGG | 0.638268 | 3:-101668104 | None:intergenic |
| ATTGAATGTACACAACTACC+TGG | 0.639305 | 3:+101666146 | MsaT016750.1:CDS |
| GCAGAAGAGACAGACTATCG+TGG | 0.640708 | 3:+101668487 | MsaT016750.1:intron |
| TACCCTCTGGGAGAGATGAG+AGG | 0.642458 | 3:-101655598 | None:intergenic |
| CTAGGAATATGTTTCCTGAG+AGG | 0.644586 | 3:-101668073 | None:intergenic |
| ATGTCGATGTTCCTCATGCG+CGG | 0.645482 | 3:+101655915 | MsaT016750.1:CDS |
| AGGAAGCTTCGAGAATATTG+GGG | 0.647727 | 3:-101659112 | None:intergenic |
| ATAAAGCATACTTGGAAGTG+GGG | 0.650220 | 3:-101657463 | None:intergenic |
| ACTCCTTTATGACGATAACC+AGG | 0.653563 | 3:-101666290 | None:intergenic |
| TGAGTCTATGGCAAAGATCG+GGG | 0.654131 | 3:-101655637 | None:intergenic |
| CGAACCCAAGGAAGTAACCA+TGG | 0.654874 | 3:+101655547 | MsaT016750.1:CDS |
| CAAGAGCAAGCAAACCCACA+CGG | 0.656542 | 3:-101666316 | None:intergenic |
| GAGACAATTTATGTTCCGAG+TGG | 0.659270 | 3:+101666170 | MsaT016750.1:CDS |
| TCAAAGTACGAGTCAACAGA+GGG | 0.660427 | 3:+101671219 | MsaT016750.1:CDS |
| TCAGTGTGTATCCAAGAGCA+GGG | 0.663517 | 3:-101669309 | None:intergenic |
| AAGGAGGTAACTAGGACACC+AGG | 0.663763 | 3:-101669451 | None:intergenic |
| GTAATCCTTCAATAAGCTAG+GGG | 0.667445 | 3:-101664535 | None:intergenic |
| AAAAGTGCCTTTAGGTGTGA+CGG | 0.668167 | 3:+101665886 | MsaT016750.1:CDS |
| AAGATATCCTCAACTGCACG+TGG | 0.672738 | 3:+101659871 | MsaT016750.1:CDS |
| GGAGAGTTGAACAATTACAA+AGG | 0.673689 | 3:+101673635 | MsaT016750.1:CDS |
| GGTTTATTGAGTGACCACGT+TGG | 0.675486 | 3:+101669420 | MsaT016750.1:CDS |
| AAGAAGTTGAGATGACAGTG+TGG | 0.676854 | 3:+101673612 | MsaT016750.1:CDS |
| AGCTGGATCAACATGCCAAG+AGG | 0.677057 | 3:-101664658 | None:intergenic |
| TGTATAAAATGTATCCAGTG+TGG | 0.684015 | 3:-101663338 | None:intergenic |
| TATCCAGTGTGGTATGACAC+CGG | 0.684470 | 3:-101663327 | None:intergenic |
| ATAAACGACCATCCTATAGG+TGG | 0.685021 | 3:+101664607 | MsaT016750.1:CDS |
| AAACTTGGCCTCCTTCCTGG+GGG | 0.689807 | 3:+101668521 | MsaT016750.1:CDS |
| TGATAATTAAGCGATGTGAG+GGG | 0.689937 | 3:+101668329 | MsaT016750.1:CDS |
| AAGTACGAGTCAACAGAGGG+TGG | 0.691127 | 3:+101671222 | MsaT016750.1:CDS |
| ATGACTCTATGCCTAAAAGG+AGG | 0.692833 | 3:+101662939 | MsaT016750.1:CDS |
| TTATACAGAGGGTGGCAATG+TGG | 0.697663 | 3:+101663354 | MsaT016750.1:CDS |
| TCATAAAGGAGTCTGCCGTG+TGG | 0.699636 | 3:+101666301 | MsaT016750.1:CDS |
| TAACCTCTCATCTCTCCCAG+AGG | 0.704903 | 3:+101655595 | MsaT016750.1:CDS |
| ATACCGGTGTCATACCACAC+TGG | 0.707579 | 3:+101663324 | MsaT016750.1:CDS |
| TAGGAATACTGGGATTCCGG+CGG | 0.710586 | 3:+101668261 | MsaT016750.1:CDS |
| CTGTATCTAAGATATCGAAG+AGG | 0.711016 | 3:-101664584 | None:intergenic |
| ACGTGAATTAAACTGGTCTG+AGG | 0.711510 | 3:+101668611 | MsaT016750.1:CDS |
| GCTTTATGGTTTAGGCACAG+AGG | 0.719101 | 3:+101667948 | MsaT016750.1:CDS |
| AGTCTAAGGAAATTGCTGAG+TGG | 0.723175 | 3:+101656008 | MsaT016750.1:CDS |
| ATACAGAAGATGCTGCCGAG+GGG | 0.739771 | 3:+101669391 | MsaT016750.1:CDS |
| CAAGAGGCACCAGATCTCTG+TGG | 0.740954 | 3:-101664642 | None:intergenic |
| CATCACAGAGAAATGCAACG+TGG | 0.742244 | 3:+101668195 | MsaT016750.1:CDS |
| AAAGATCACTGAAATCAAGG+AGG | 0.742693 | 3:-101669467 | None:intergenic |
| ACCAGGATTTCACTCCAACG+TGG | 0.759662 | 3:-101669434 | None:intergenic |
| ACCCAAGGAAGTAACCATGG+CGG | 0.764946 | 3:+101655550 | MsaT016750.1:CDS |
| GATGTGGTAAAAGATGAAGG+TGG | 0.808325 | 3:-101668554 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 101655458 | 101673667 | 101655458 | ID=MsaG016750 |
| Chr3 | mRNA | 101655458 | 101673667 | 101655458 | ID=MsaT016750.1;Parent=MsaG016750 |
| Chr3 | exon | 101655458 | 101655694 | 101655458 | ID=MsaT016750.1.exon1;Parent=MsaT016750.1 |
| Chr3 | CDS | 101655458 | 101655694 | 101655458 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101655899 | 101656147 | 101655899 | ID=MsaT016750.1.exon2;Parent=MsaT016750.1 |
| Chr3 | CDS | 101655899 | 101656147 | 101655899 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101657396 | 101657474 | 101657396 | ID=MsaT016750.1.exon3;Parent=MsaT016750.1 |
| Chr3 | CDS | 101657396 | 101657474 | 101657396 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101658902 | 101658989 | 101658902 | ID=MsaT016750.1.exon4;Parent=MsaT016750.1 |
| Chr3 | CDS | 101658902 | 101658989 | 101658902 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101659086 | 101659145 | 101659086 | ID=MsaT016750.1.exon5;Parent=MsaT016750.1 |
| Chr3 | CDS | 101659086 | 101659145 | 101659086 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101659842 | 101659926 | 101659842 | ID=MsaT016750.1.exon6;Parent=MsaT016750.1 |
| Chr3 | CDS | 101659842 | 101659926 | 101659842 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101662192 | 101662307 | 101662192 | ID=MsaT016750.1.exon7;Parent=MsaT016750.1 |
| Chr3 | CDS | 101662192 | 101662307 | 101662192 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101662902 | 101663015 | 101662902 | ID=MsaT016750.1.exon8;Parent=MsaT016750.1 |
| Chr3 | CDS | 101662902 | 101663015 | 101662902 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101663112 | 101663170 | 101663112 | ID=MsaT016750.1.exon9;Parent=MsaT016750.1 |
| Chr3 | CDS | 101663112 | 101663170 | 101663112 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101663293 | 101663435 | 101663293 | ID=MsaT016750.1.exon10;Parent=MsaT016750.1 |
| Chr3 | CDS | 101663293 | 101663435 | 101663293 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101664196 | 101664393 | 101664196 | ID=MsaT016750.1.exon11;Parent=MsaT016750.1 |
| Chr3 | CDS | 101664196 | 101664393 | 101664196 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101664522 | 101664721 | 101664522 | ID=MsaT016750.1.exon12;Parent=MsaT016750.1 |
| Chr3 | CDS | 101664522 | 101664721 | 101664522 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101665865 | 101665964 | 101665865 | ID=MsaT016750.1.exon13;Parent=MsaT016750.1 |
| Chr3 | CDS | 101665865 | 101665964 | 101665865 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101666101 | 101666676 | 101666101 | ID=MsaT016750.1.exon14;Parent=MsaT016750.1 |
| Chr3 | CDS | 101666101 | 101666676 | 101666101 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101666771 | 101666893 | 101666771 | ID=MsaT016750.1.exon15;Parent=MsaT016750.1 |
| Chr3 | CDS | 101666771 | 101666893 | 101666771 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101667889 | 101667969 | 101667889 | ID=MsaT016750.1.exon16;Parent=MsaT016750.1 |
| Chr3 | CDS | 101667889 | 101667969 | 101667889 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101668037 | 101668368 | 101668037 | ID=MsaT016750.1.exon17;Parent=MsaT016750.1 |
| Chr3 | CDS | 101668037 | 101668368 | 101668037 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101668491 | 101668748 | 101668491 | ID=MsaT016750.1.exon18;Parent=MsaT016750.1 |
| Chr3 | CDS | 101668491 | 101668748 | 101668491 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101669101 | 101669185 | 101669101 | ID=MsaT016750.1.exon19;Parent=MsaT016750.1 |
| Chr3 | CDS | 101669101 | 101669185 | 101669101 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101669285 | 101669495 | 101669285 | ID=MsaT016750.1.exon20;Parent=MsaT016750.1 |
| Chr3 | CDS | 101669285 | 101669495 | 101669285 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101671109 | 101671277 | 101671109 | ID=MsaT016750.1.exon21;Parent=MsaT016750.1 |
| Chr3 | CDS | 101671109 | 101671277 | 101671109 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Chr3 | exon | 101673529 | 101673667 | 101673529 | ID=MsaT016750.1.exon22;Parent=MsaT016750.1 |
| Chr3 | CDS | 101673529 | 101673667 | 101673529 | ID=cds.MsaT016750.1;Parent=MsaT016750.1 |
| Gene Sequence |
| Protein sequence |
