AlfalfaGEDB | MsaG016764

M. sativa ssp. caerulea / MsaG016764

KEGG: K18485
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016764	XP_003602850.1	94.190	327	11	2	1	325	1	321	0.0	635

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016764	sp\|Q9C7T4\|BH096_ARATH	49.560	341	131	9	1	322	1	319	4.15e-90	275

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016764	F0V437	94.190	327	11	2	1	325	1	321	0.0	635

TFs/TRs:

Gene ID	Type	Classification
MsaG016764	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000070	MsaG016764	0.806866	1.133697e-49	1.795688e-47
MsaG000113	MsaG016764	0.804367	3.771222e-49	5.634151e-47
MsaG001724	MsaG016764	0.801410	1.529241e-48	2.134529e-46
MsaG002814	MsaG016764	0.800732	2.100738e-48	2.887703e-46
MsaG002878	MsaG016764	0.832491	1.659082e-55	5.160113e-53
MsaG003136	MsaG016764	0.808479	5.170774e-50	8.512267e-48
MsaG004157	MsaG016764	0.820928	9.289641e-53	2.093965e-50
MsaG004159	MsaG016764	0.820129	1.414520e-52	3.121756e-50
MsaG004848	MsaG016764	0.812796	6.089961e-51	1.113937e-48
MsaG005035	MsaG016764	0.802792	7.971950e-49	1.148351e-46
MsaG005003	MsaG016764	0.808608	4.854455e-50	8.016241e-48
MsaG005138	MsaG016764	0.818035	4.214981e-52	8.805509e-50
MsaG005368	MsaG016764	0.814508	2.569130e-51	4.905055e-49
MsaG005379	MsaG016764	0.823081	2.961443e-53	7.071026e-51
MsaG006576	MsaG016764	0.801714	1.325506e-48	1.862956e-46
MsaG006612	MsaG016764	0.826946	3.658570e-54	9.713615e-52
MsaG008682	MsaG016764	0.805107	2.645941e-49	4.021395e-47
MsaG009005	MsaG016764	0.816921	7.488651e-52	1.520259e-49
MsaG009453	MsaG016764	0.809467	3.183684e-50	5.367537e-48
MsaG010594	MsaG016764	0.800299	2.571649e-48	3.500472e-46
MsaG010943	MsaG016764	0.814441	2.657109e-51	5.064724e-49
MsaG010999	MsaG016764	0.800722	2.110801e-48	2.900889e-46
MsaG012454	MsaG016764	0.815076	1.924878e-51	3.728038e-49
MsaG012610	MsaG016764	0.804938	2.869074e-49	4.343449e-47
MsaG016220	MsaG016764	0.813468	4.345361e-51	8.082808e-49
MsaG016516	MsaG016764	0.823885	1.925265e-53	4.698000e-51
MsaG016764	MsaG017072	0.814971	2.030205e-51	3.921800e-49
MsaG016764	MsaG018066	0.827675	2.451038e-54	6.641861e-52
MsaG016764	MsaG018069	0.822694	3.642512e-53	8.606322e-51
MsaG016764	MsaG018484	0.806292	1.496616e-49	2.338753e-47
MsaG016764	MsaG019533	0.806553	1.319129e-49	2.074109e-47
MsaG016764	MsaG019589	0.802404	9.575186e-49	1.367175e-46
MsaG016764	MsaG019716	0.811628	1.092370e-50	1.941158e-48
MsaG016764	MsaG019865	0.803163	6.686897e-49	9.715459e-47
MsaG016764	MsaG020202	0.804974	2.820789e-49	4.273891e-47
MsaG016764	MsaG020712	0.813539	4.192851e-51	7.813005e-49
MsaG016764	MsaG021046	0.810052	2.386999e-50	4.081413e-48
MsaG016764	MsaG022548	0.802411	9.546354e-49	1.363245e-46
MsaG016764	MsaG023475	0.800617	2.216733e-48	3.039290e-46
MsaG016764	MsaG023967	0.818130	4.012164e-52	8.402469e-50
MsaG016764	MsaG025416	0.802098	1.106173e-48	1.568323e-46
MsaG016764	MsaG026823	0.802775	8.034836e-49	1.156965e-46
MsaG016764	MsaG027107	0.800016	2.933758e-48	3.968190e-46
MsaG016764	MsaG027573	0.804711	3.198586e-49	4.816773e-47
MsaG016764	MsaG027595	0.801643	1.370612e-48	1.923271e-46
MsaG016764	MsaG027991	0.804337	3.825118e-49	5.710780e-47
MsaG016764	MsaG029822	0.805734	1.958517e-49	3.020397e-47
MsaG016764	MsaG030246	0.817751	4.881363e-52	1.012311e-49
MsaG016764	MsaG035456	0.805317	2.392892e-49	3.654578e-47
MsaG016764	MsaG040566	0.810867	1.594783e-50	2.781565e-48
MsaG016764	MsaG042575	0.812895	5.794727e-51	1.062544e-48
MsaG016764	MsaG044627	0.824331	1.514663e-53	3.741343e-51
MsaG016764	MsaG045061	0.815678	1.416520e-51	2.785676e-49
MsaG016764	MsaG045517	0.809668	2.884403e-50	4.886363e-48
MsaG016764	MsaG045601	0.802045	1.134155e-48	1.606068e-46
MsaG016764	MsaG045724	0.816960	7.340012e-52	1.491561e-49
MsaG016764	MsaG046289	0.824953	1.082196e-53	2.718824e-51
MsaG016764	MsaG047030	0.800143	2.765482e-48	3.751102e-46
MsaG016764	MsaG004516	0.804695	3.223114e-49	4.851903e-47
MsaG016764	MsaG002237	0.809260	3.525758e-50	5.914100e-48
MsaG016764	MsaG009147	0.802554	8.919855e-49	1.277975e-46
MsaG016764	MsaG008719	0.802345	9.847469e-49	1.404141e-46
MsaG016764	MsaG010534	0.800121	2.794028e-48	3.787931e-46
MsaG016764	MsaG016849	0.811123	1.404373e-50	2.464906e-48
MsaG016764	MsaG018908	0.818109	4.056665e-52	8.491111e-50
MsaG016764	MsaG018951	0.818706	2.975162e-52	6.324293e-50
MsaG016764	MsaG025429	0.806234	1.538732e-49	2.401265e-47
MsaG016764	MsaG030575	0.804326	3.844998e-49	5.738971e-47
MsaG016764	MsaG031628	0.803396	5.988445e-49	8.747470e-47
MsaG016764	MsaG035285	0.815661	1.429048e-51	2.809103e-49
MsaG016764	MsaG035348	0.806301	1.489708e-49	2.328453e-47
MsaG016764	MsaG041375	0.805379	2.322748e-49	3.552539e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016764	MtrunA17_Chr3g0132471	94.190	327	11	2	1	325	1	321	0.0	635
MsaG016764	MtrunA17_Chr5g0426851	56.098	328	106	10	1	321	1	297	3.44e-107	315
MsaG016764	MtrunA17_Chr7g0273081	51.250	320	131	6	1	319	14	309	1.81e-96	288
MsaG016764	MtrunA17_Chr1g0191571	50.755	331	140	6	1	319	14	333	1.49e-93	281
MsaG016764	MtrunA17_Chr8g0366551	57.674	215	74	3	111	319	113	316	2.54e-80	247
MsaG016764	MtrunA17_Chr8g0345871	52.427	206	84	3	111	316	170	361	2.11e-58	192
MsaG016764	MtrunA17_Chr0c01g0489461	46.930	228	96	6	103	316	176	392	1.27e-56	188
MsaG016764	MtrunA17_Chr2g0287761	41.146	192	102	4	122	311	1	183	9.55e-40	138
MsaG016764	MtrunA17_Chr1g0158941	36.437	247	127	7	85	311	54	290	4.24e-36	132
MsaG016764	MtrunA17_Chr3g0126381	27.778	198	105	5	151	311	1	197	1.14e-15	74.3
MsaG016764	MtrunA17_Chr3g0126361	57.143	56	20	1	102	153	77	132	1.35e-12	64.7
MsaG016764	MtrunA17_Chr1g0211081	26.066	211	122	7	125	314	319	516	1.39e-12	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG016764	AT1G72210.1	49.560	341	131	9	1	322	1	319	4.22e-91	275
MsaG016764	AT1G22490.1	46.084	332	137	9	1	319	1	303	1.13e-85	260
MsaG016764	AT1G72210.2	48.718	312	119	10	1	293	1	290	9.17e-76	235
MsaG016764	AT1G22490.2	44.194	310	131	9	1	297	1	281	1.37e-70	223
MsaG016764	AT4G01460.1	41.784	213	118	1	110	322	103	309	2.98e-53	177
MsaG016764	AT4G01460.2	41.784	213	118	1	110	322	103	309	2.98e-53	177
MsaG016764	AT5G65320.1	47.059	204	100	3	118	319	98	295	4.66e-52	174
MsaG016764	AT3G24140.2	43.612	227	111	4	101	316	176	396	6.24e-52	176
MsaG016764	AT3G24140.1	43.612	227	111	4	101	316	176	396	7.76e-52	176
MsaG016764	AT2G46810.2	46.040	202	92	3	113	313	112	297	1.04e-50	170
MsaG016764	AT2G46810.3	46.040	202	92	3	113	313	172	357	2.57e-50	171
MsaG016764	AT2G46810.1	46.040	202	92	3	113	313	184	369	3.01e-50	171
MsaG016764	AT5G46690.1	43.802	242	87	7	111	319	77	302	3.42e-49	167
MsaG016764	AT5G65320.2	47.568	185	90	2	110	293	90	268	1.72e-47	161
MsaG016764	AT3G61950.2	45.098	204	94	3	111	313	116	302	2.12e-47	162
MsaG016764	AT3G61950.1	45.098	204	94	3	111	313	167	353	4.10e-47	162
MsaG016764	AT2G46810.4	40.870	230	91	4	113	313	112	325	3.44e-45	157
MsaG016764	AT3G61950.6	46.471	170	74	2	111	280	116	268	5.58e-43	150
MsaG016764	AT3G61950.3	46.471	170	74	2	111	280	167	319	1.06e-42	151
MsaG016764	AT5G46690.2	43.056	216	74	7	111	293	77	276	1.41e-38	138
MsaG016764	AT3G06120.1	36.683	199	108	5	122	311	1	190	5.01e-32	119
MsaG016764	AT5G53210.1	66.154	65	22	0	120	184	100	164	5.50e-23	98.2
MsaG016764	AT5G53210.2	66.154	65	22	0	120	184	100	164	6.02e-23	97.8
MsaG016764	AT3G61950.4	69.767	43	13	0	111	153	167	209	9.23e-14	69.7
MsaG016764	AT3G61950.5	69.767	43	13	0	111	153	167	209	9.23e-14	69.7

Find 68 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGCCCATTGTTTGTGTTCT+TGG	0.102767	3:+101847370	None:intergenic
AGAACAACATTTACAGTCTA+TGG	0.205160	3:-101847538	MsaT016764.1:CDS
TGATCAACGGTGCAGATTTC+AGG	0.214308	3:+101848744	None:intergenic
GGTGACCAAGCTTCTATTAT+TGG	0.242168	3:-101847588	MsaT016764.1:CDS
TTGTTGGGTTACAAAATCTT+AGG	0.275677	3:-101847260	MsaT016764.1:CDS
AACAAAATCTCCATGCAAAC+TGG	0.290252	3:-101848818	MsaT016764.1:CDS
CTGGGATTCTTGTTCTGTTA+AGG	0.317030	3:-101848799	MsaT016764.1:CDS
TGTTACCACGGTCGATGATA+TGG	0.322275	3:-101847217	MsaT016764.1:CDS
CTGCTGTGAATCAACTATTA+AGG	0.328683	3:-101846998	MsaT016764.1:CDS
TTTAAGCCTATGTTCTCGTT+TGG	0.389954	3:+101847492	None:intergenic
TGCCTTCTTCTTATGTTCAA+AGG	0.395209	3:-101848530	MsaT016764.1:CDS
GTTGATAAGCCAGTTCTTGT+TGG	0.407779	3:+101846970	None:intergenic
GAGAACATAGGCTTAAATAA+TGG	0.421439	3:-101847486	MsaT016764.1:CDS
AACTTATGAAGATTGTTGTT+GGG	0.423130	3:-101847275	MsaT016764.1:CDS
GCCTTCTTCTTATGTTCAAA+GGG	0.428871	3:-101848529	MsaT016764.1:intron
CAACTTATGAAGATTGTTGT+TGG	0.446451	3:-101847276	MsaT016764.1:CDS
TTCTGTTAAGGATCATCAAT+GGG	0.447040	3:-101848787	MsaT016764.1:CDS
AAGGATGGTCCAACAAGAAC+TGG	0.453481	3:-101846979	MsaT016764.1:CDS
TGCAGTTGCTGTTGCTTCCA+CGG	0.462288	3:+101848692	None:intergenic
GTTCATTGTTGATGATTCCT+AGG	0.463558	3:+101848841	None:intergenic
GAACAACATTTACAGTCTAT+GGG	0.463577	3:-101847537	MsaT016764.1:CDS
GTTCTGTTAAGGATCATCAA+TGG	0.470917	3:-101848788	MsaT016764.1:CDS
TGATTGGAGCAGGTAGAGGA+GGG	0.476683	3:-101847053	MsaT016764.1:intron
ACAATCTTCATAAGTTGTCC+TGG	0.483622	3:+101847282	None:intergenic
TTGATTGGAGCAGGTAGAGG+AGG	0.489389	3:-101847054	MsaT016764.1:intron
CTCCTCCAATAATAGAAGCT+TGG	0.501188	3:+101847583	None:intergenic
TCTTTGTAACTATATGTGAA+TGG	0.514401	3:+101848930	None:intergenic
AACAAGAATCCCAGTTTGCA+TGG	0.514438	3:+101848808	None:intergenic
AAAGTGAAGAATTCAGCAAA+AGG	0.515194	3:+101847459	None:intergenic
GGTAACATTAAGATGAAGAA+TGG	0.520032	3:+101847233	None:intergenic
GTTGCTTCCACGGTGGAAGA+AGG	0.520106	3:+101848702	None:intergenic
GACCAAGCTTCTATTATTGG+AGG	0.520738	3:-101847585	MsaT016764.1:CDS
AAGAGGAGATTGAAAATCAA+AGG	0.536216	3:-101848626	MsaT016764.1:CDS
GAACAAGAGAATGTTCTCCT+AGG	0.539799	3:-101848858	MsaT016764.1:CDS
TTACCAAGAACACAAACAAT+GGG	0.539923	3:-101847373	MsaT016764.1:CDS
ACCCTTTGAACATAAGAAGA+AGG	0.558671	3:+101848528	None:intergenic
TCTTTACTCAGTTAGTGTCA+AGG	0.560473	3:-101847193	MsaT016764.1:intron
GATTGGAGCAGGTAGAGGAG+GGG	0.565920	3:-101847052	MsaT016764.1:intron
ATTACCAAGAACACAAACAA+TGG	0.566449	3:-101847374	MsaT016764.1:CDS
AATAATGGCTCTTGAAACTG+TGG	0.572614	3:-101848970	None:intergenic
ATGTGAATGGATCTTGTTGT+GGG	0.578863	3:+101848943	None:intergenic
ACAAAATCTCCATGCAAACT+GGG	0.583052	3:-101848817	MsaT016764.1:CDS
TGTGAATCAACTATTAAGGA+TGG	0.590311	3:-101846994	MsaT016764.1:CDS
CACCGTTGATCAAACTATCA+CGG	0.598610	3:-101848733	MsaT016764.1:CDS
AGGAGCAATAAACTTTGTGA+AGG	0.602345	3:-101847565	MsaT016764.1:CDS
CAAGAGTGCAAAGAACAAAG+AGG	0.609385	3:-101848643	MsaT016764.1:CDS
AAACAACCAAACGAGAACAT+AGG	0.609692	3:-101847498	MsaT016764.1:CDS
AACGGTGCAGATTTCAGGAG+AGG	0.610812	3:+101848749	None:intergenic
GGGGAGTCAGCTGAATAGTG+TGG	0.613845	3:-101847033	MsaT016764.1:CDS
TCCACGGTGGAAGAAGGAGG+AGG	0.617851	3:+101848708	None:intergenic
TCTTCATCTTAATGTTACCA+CGG	0.620808	3:-101847229	MsaT016764.1:CDS
CAACAACAACAACGTCACAA+TGG	0.620930	3:-101847406	MsaT016764.1:CDS
AACAACATTTACAGTCTATG+GGG	0.630582	3:-101847536	MsaT016764.1:CDS
CAACAGCAACTGCAACGTGT+AGG	0.632313	3:-101848683	MsaT016764.1:CDS
GCTTCCACGGTGGAAGAAGG+AGG	0.636333	3:+101848705	None:intergenic
AGCCGTGATAGTTTGATCAA+CGG	0.636568	3:+101848731	None:intergenic
GGCAGACATTGAAGTTACAA+TGG	0.641899	3:-101847346	MsaT016764.1:CDS
GTGAAGAATTCAGCAAAAGG+TGG	0.646381	3:+101847462	None:intergenic
AGAACACAAACAATGGGCTG+TGG	0.648246	3:-101847367	MsaT016764.1:CDS
TATGTGAATGGATCTTGTTG+TGG	0.651367	3:+101848942	None:intergenic
ACAACATTTACAGTCTATGG+GGG	0.663491	3:-101847535	MsaT016764.1:CDS
GAATAGTGTGGATGAAATTG+CGG	0.673702	3:-101847021	MsaT016764.1:CDS
TGACTATGATCATCTTCATG+CGG	0.681857	3:-101848883	MsaT016764.1:CDS
GTTGCACTTGTTGTGTACTG+AGG	0.689048	3:+101847435	None:intergenic
TCCTCCTCCTTCTTCCACCG+TGG	0.691097	3:-101848709	MsaT016764.1:CDS
AAGAACCATATCATCGACCG+TGG	0.707791	3:+101847212	None:intergenic
CAACATTTACAGTCTATGGG+GGG	0.730568	3:-101847534	MsaT016764.1:CDS
AGTTGCTGTTGCTTCCACGG+TGG	0.802421	3:+101848695	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	101846962	101848988	101846962	ID=MsaG016764
Chr3	mRNA	101846962	101848988	101846962	ID=MsaT016764.1;Parent=MsaG016764
Chr3	exon	101846962	101847063	101846962	ID=MsaT016764.1.exon3;Parent=MsaT016764.1
Chr3	CDS	101846962	101847063	101846962	ID=cds.MsaT016764.1;Parent=MsaT016764.1
Chr3	exon	101847194	101847610	101847194	ID=MsaT016764.1.exon2;Parent=MsaT016764.1
Chr3	CDS	101847194	101847610	101847194	ID=cds.MsaT016764.1;Parent=MsaT016764.1
Chr3	exon	101848530	101848988	101848530	ID=MsaT016764.1.exon1;Parent=MsaT016764.1
Chr3	CDS	101848530	101848988	101848530	ID=cds.MsaT016764.1;Parent=MsaT016764.1

Gene Sequence

>MsaT016764.1

ATGGCTCTTGAAACTGTGGTTTTCCCACAACAAGATCCATTCACATATAGTTACAAAGACAATTACTTCAACTCTTTGAACAATGACTATGATCATCTTCATGCGGAAGAACAAGAGAATGTTCTCCTAGGAATCATCAACAATGAACAACAAAATCTCCATGCAAACTGGGATTCTTGTTCTGTTAAGGATCATCAATGGGAATATTCACACTCTTCCTCTCCTGAAATCTGCACCGTTGATCAAACTATCACGGCTCCTCCTCCTTCTTCCACCGTGGAAGCAACAGCAACTGCAACGTGTAGGAGAAAGCGACGTCGTATCAAGAGTGCAAAGAACAAAGAGGAGATTGAAAATCAAAGGATGACTCATATTACTGTTGAACGCAATCGAAGAAAACAAATGAATGAATATTTGAATGTGCTTCGATCTTTGATGCCTTCTTCTTATGTTCAAAGGGGTGACCAAGCTTCTATTATTGGAGGAGCAATAAACTTTGTGAAGGAGTTAGAACAACATTTACAGTCTATGGGGGGCCAAAAGAAAACGAAACAACCAAACGAGAACATAGGCTTAAATAATGGACCACCTTTTGCTGAATTCTTCACTTTTCCTCAGTACACAACAAGTGCAACTCAGAACAACAACAACAACGTCACAATGGAACAACACAATTACCAAGAACACAAACAATGGGCTGTGGCAGACATTGAAGTTACAATGGTAGATAGTCATGCCAACATGAAAATTCTATCCAAGAAAAAACCAGGACAACTTATGAAGATTGTTGTTGGGTTACAAAATCTTAGGCTTACCATTCTTCATCTTAATGTTACCACGGTCGATGATATGGTTCTTTACTCAGTTAGTGTCAAGGTAGAGGAGGGGAGTCAGCTGAATAGTGTGGATGAAATTGCGGCTGCTGTGAATCAACTATTAAGGATGGTCCAACAAGAACTGGCTTATCAACTAAATTGA

Protein sequence

>MsaT016764.1

MALETVVFPQQDPFTYSYKDNYFNSLNNDYDHLHAEEQENVLLGIINNEQQNLHANWDSCSVKDHQWEYSHSSSPEICTVDQTITAPPPSSTVEATATATCRRKRRRIKSAKNKEEIENQRMTHITVERNRRKQMNEYLNVLRSLMPSSYVQRGDQASIIGGAINFVKELEQHLQSMGGQKKTKQPNENIGLNNGPPFAEFFTFPQYTTSATQNNNNNVTMEQHNYQEHKQWAVADIEVTMVDSHANMKILSKKKPGQLMKIVVGLQNLRLTILHLNVTTVDDMVLYSVSVKVEEGSQLNSVDEIAAAVNQLLRMVQQELAYQLN*