AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016818


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016818 MtrunA17_Chr3g0134741 89.720 321 27 1 1 315 1 321 0.0 599
MsaG016818 MtrunA17_Chr1g0152651 48.501 367 102 11 1 298 1 349 6.18e-86 262
MsaG016818 MtrunA17_Chr5g0393721 40.356 337 152 14 1 313 1 312 6.19e-56 184
MsaG016818 MtrunA17_Chr1g0207301 51.445 173 63 4 147 308 211 373 3.66e-46 160
MsaG016818 MtrunA17_Chr1g0207311 78.161 87 19 0 222 308 195 281 2.33e-43 150
MsaG016818 MtrunA17_Chr5g0435021 58.571 70 29 0 222 291 217 286 1.58e-22 95.5
MsaG016818 MtrunA17_Chr4g0074161 52.632 76 36 0 222 297 205 280 2.56e-18 84.7
MsaG016818 MtrunA17_Chr8g0384141 60.606 66 25 1 222 287 114 178 1.14e-17 81.3
MsaG016818 MtrunA17_Chr1g0190731 46.341 82 44 0 222 303 192 273 3.68e-16 78.2
MsaG016818 MtrunA17_Chr3g0134001 53.968 63 29 0 223 285 171 233 7.54e-16 76.3
MsaG016818 MtrunA17_Chr7g0243041 54.545 55 25 0 230 284 33 87 7.97e-15 70.9
MsaG016818 MtrunA17_Chr1g0153241 34.167 120 73 2 181 300 49 162 9.22e-14 70.1
MsaG016818 MtrunA17_Chr1g0193321 45.455 66 36 0 221 286 460 525 3.33e-13 70.1
MsaG016818 MtrunA17_Chr4g0014771 53.030 66 30 1 222 286 117 182 9.41e-13 65.9
MsaG016818 MtrunA17_Chr8g0363921 31.847 157 88 5 139 286 69 215 2.98e-12 65.9
MsaG016818 MtrunA17_Chr1g0182791 43.939 66 37 0 221 286 315 380 4.44e-12 66.6
MsaG016818 MtrunA17_Chr4g0032951 39.823 113 66 2 175 286 308 419 4.65e-12 66.2
MsaG016818 MtrunA17_Chr3g0095391 30.822 146 89 2 154 292 233 373 5.01e-12 66.2
MsaG016818 MtrunA17_Chr6g0484271 44.318 88 44 2 204 286 132 219 6.80e-12 64.7
MsaG016818 MtrunA17_Chr7g0234761 30.526 190 116 5 98 286 207 381 9.44e-12 65.5
MsaG016818 MtrunA17_Chr1g0174271 54.545 66 29 1 222 286 297 362 1.04e-11 65.1
MsaG016818 MtrunA17_Chr5g0402031 42.188 64 37 0 236 299 118 181 1.47e-11 64.3
MsaG016818 MtrunA17_Chr8g0339091 53.125 64 29 1 224 286 292 355 1.48e-11 65.1
MsaG016818 MtrunA17_Chr1g0152131 51.562 64 30 1 224 286 261 324 2.18e-11 64.3
MsaG016818 MtrunA17_Chr7g0270871 46.269 67 34 1 220 286 201 265 2.27e-11 63.9
MsaG016818 MtrunA17_Chr5g0415291 44.565 92 42 3 201 286 88 176 2.74e-11 62.8
MsaG016818 MtrunA17_Chr4g0035101 51.562 64 30 1 224 286 144 207 4.25e-11 63.2
MsaG016818 MtrunA17_Chr7g0231461 50.000 60 28 1 226 285 302 359 5.90e-11 63.2
MsaG016818 MtrunA17_Chr3g0131771 53.125 64 29 1 224 286 148 211 6.08e-11 62.4
MsaG016818 MtrunA17_Chr3g0144641 51.562 64 30 1 224 286 192 255 7.08e-11 62.8
MsaG016818 MtrunA17_Chr2g0329041 51.562 64 30 1 222 284 331 394 7.18e-11 62.8
MsaG016818 MtrunA17_Chr5g0421111 51.562 64 30 1 224 286 366 429 7.28e-11 62.8
MsaG016818 MtrunA17_Chr8g0365941 51.562 64 30 1 224 286 306 369 9.21e-11 62.4
MsaG016818 MtrunA17_Chr7g0262691 36.559 93 54 1 191 283 225 312 9.73e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016818 AT2G14760.1 50.230 217 74 6 109 307 121 321 2.91e-56 185
MsaG016818 AT4G33880.1 59.119 159 62 1 150 305 190 348 1.16e-54 182
MsaG016818 AT1G27740.1 61.486 148 40 3 162 308 121 252 3.92e-53 175
MsaG016818 AT2G14760.3 47.391 230 74 7 109 307 121 334 1.22e-52 176
MsaG016818 AT5G43175.1 57.931 145 52 3 165 308 81 217 9.80e-46 155
MsaG016818 AT5G37800.1 61.039 77 29 1 216 291 193 269 1.31e-23 99.0
MsaG016818 AT1G66470.1 52.809 89 42 0 203 291 175 263 2.34e-23 97.8
MsaG016818 AT2G24260.2 51.613 93 43 1 207 297 17 109 1.20e-21 92.4
MsaG016818 AT4G30980.1 47.573 103 47 2 189 290 101 197 3.53e-21 92.0
MsaG016818 AT4G30980.2 47.573 103 47 2 189 290 149 245 4.09e-21 92.8
MsaG016818 AT2G24260.1 51.613 93 43 1 207 297 120 212 7.04e-21 92.0
MsaG016818 AT5G58010.1 46.875 96 47 2 222 313 98 193 2.39e-19 87.0
MsaG016818 AT3G21330.1 52.381 63 30 0 223 285 269 331 2.72e-15 76.3
MsaG016818 AT5G50915.3 34.637 179 77 7 170 314 57 229 8.20e-15 73.6
MsaG016818 AT5G50915.4 34.637 179 77 7 170 314 57 229 8.20e-15 73.6
MsaG016818 AT5G50915.1 34.637 179 77 7 170 314 57 229 8.49e-15 73.9
MsaG016818 AT5G50915.2 34.637 179 77 7 170 314 57 229 8.49e-15 73.9
MsaG016818 AT5G01305.1 49.180 61 31 0 222 282 36 96 1.35e-14 70.9
MsaG016818 AT4G00120.1 50.000 54 27 0 232 285 121 174 3.36e-14 70.5
MsaG016818 AT1G25330.1 50.617 81 39 1 224 303 105 185 4.87e-13 67.8
MsaG016818 AT1G09530.3 37.895 95 53 1 201 295 321 409 6.76e-13 69.3
MsaG016818 AT1G09530.2 37.895 95 53 1 201 295 321 409 6.76e-13 69.3
MsaG016818 AT1G09530.5 37.895 95 53 1 201 295 321 409 6.76e-13 69.3
MsaG016818 AT1G09530.1 37.895 95 53 1 201 295 321 409 6.76e-13 69.3
MsaG016818 AT1G09530.4 37.895 95 53 1 201 295 321 409 6.76e-13 69.3
MsaG016818 AT1G09530.6 37.895 95 53 1 201 295 321 409 6.76e-13 69.3
MsaG016818 AT4G00050.2 39.423 104 51 3 183 286 77 168 1.06e-12 67.8
MsaG016818 AT1G51140.1 53.846 65 29 1 222 285 303 367 1.14e-12 68.6
MsaG016818 AT2G20180.7 32.031 128 69 3 196 314 185 303 1.25e-12 68.6
MsaG016818 AT2G20180.1 32.031 128 69 3 196 314 185 303 1.25e-12 68.6
MsaG016818 AT2G20180.5 32.031 128 69 3 196 314 185 303 1.25e-12 68.6
MsaG016818 AT2G20180.4 32.031 128 69 3 196 314 256 374 1.58e-12 68.2
MsaG016818 AT2G20180.6 32.031 128 69 3 196 314 256 374 1.58e-12 68.2
MsaG016818 AT2G20180.2 32.031 128 69 3 196 314 256 374 1.58e-12 68.2
MsaG016818 AT2G20180.3 32.031 128 69 3 196 314 256 374 1.58e-12 68.2
MsaG016818 AT1G18400.2 54.688 64 28 1 224 286 146 209 1.76e-12 66.6
MsaG016818 AT4G00050.3 39.423 104 51 3 183 286 179 270 2.32e-12 67.4
MsaG016818 AT1G18400.1 54.688 64 28 1 224 286 146 209 2.41e-12 66.2
MsaG016818 AT4G00050.1 39.423 104 51 3 183 286 179 270 2.42e-12 67.4
MsaG016818 AT4G09180.1 48.529 68 34 1 222 288 183 250 4.37e-12 65.9
MsaG016818 AT2G46970.2 30.645 124 80 2 195 313 165 287 4.40e-12 66.6
MsaG016818 AT2G46970.3 30.645 124 80 2 195 313 174 296 4.79e-12 66.6
MsaG016818 AT2G46970.1 30.645 124 80 2 195 313 196 318 4.95e-12 66.6
MsaG016818 AT1G35460.1 50.000 66 32 1 222 286 180 245 8.73e-12 64.7
MsaG016818 AT1G73830.2 51.562 64 30 1 224 286 148 211 1.29e-11 64.3
MsaG016818 AT4G28790.1 45.946 74 39 1 227 300 275 347 1.32e-11 65.5
MsaG016818 AT1G73830.1 51.562 64 30 1 224 286 148 211 1.33e-11 64.3
MsaG016818 AT4G28815.2 38.776 98 55 2 206 298 47 144 1.37e-11 63.9
MsaG016818 AT4G28815.1 38.776 98 55 2 206 298 123 220 1.89e-11 64.3
MsaG016818 AT2G18300.2 36.434 129 73 3 159 286 129 249 2.07e-11 64.3
MsaG016818 AT5G67110.1 40.404 99 45 3 219 315 81 167 2.20e-11 62.8
MsaG016818 AT2G18300.1 36.434 129 73 3 159 286 129 249 2.23e-11 64.3
MsaG016818 AT1G68920.3 32.237 152 101 2 136 286 216 366 2.30e-11 64.7
MsaG016818 AT2G18300.3 36.434 129 73 3 159 286 129 249 2.48e-11 64.3
MsaG016818 AT3G07340.1 53.125 64 29 1 224 286 259 322 3.02e-11 64.3
MsaG016818 AT4G36930.1 35.294 85 55 0 230 314 198 282 3.17e-11 63.9
MsaG016818 AT3G07340.2 53.125 64 29 1 224 286 259 322 3.41e-11 64.3
MsaG016818 AT1G26260.3 53.125 64 29 1 224 286 173 236 4.15e-11 63.5
MsaG016818 AT2G42280.1 52.308 65 30 1 222 285 278 342 4.19e-11 63.5
MsaG016818 AT1G26260.4 53.125 64 29 1 224 286 224 287 4.40e-11 63.5
MsaG016818 AT1G26260.1 53.125 64 29 1 224 286 224 287 4.40e-11 63.5
MsaG016818 AT1G26260.6 53.125 64 29 1 224 286 224 287 4.40e-11 63.5
MsaG016818 AT1G26260.2 53.125 64 29 1 224 286 224 287 4.40e-11 63.5
MsaG016818 AT5G48560.1 53.125 64 29 1 224 286 302 365 4.42e-11 63.9
MsaG016818 AT5G48560.2 53.125 64 29 1 224 286 302 365 4.89e-11 63.9
MsaG016818 AT1G26260.5 53.125 64 29 1 224 286 202 265 4.97e-11 63.5
MsaG016818 AT1G10120.2 51.562 64 30 1 224 286 207 270 5.17e-11 63.5
MsaG016818 AT1G10120.1 51.562 64 30 1 224 286 207 270 5.17e-11 63.5
MsaG016818 AT1G59640.1 50.000 64 31 1 224 286 137 200 5.25e-11 62.4
MsaG016818 AT2G20180.8 37.079 89 51 1 196 284 256 339 6.67e-11 62.8
MsaG016818 AT4G34530.1 51.562 64 30 1 224 286 173 236 8.21e-11 62.8

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACCTTCTTGCTCTTCT+TGG 0.125181 3:+102536884 None:intergenic
GCTTGATGTGCCAGAATTTC+AGG 0.228928 3:-102537201 MsaT016818.1:CDS
GCTGCAGAAATTTCACATAT+TGG 0.264015 3:+102536236 None:intergenic
CGACTCATATTACCTTGATT+TGG 0.285078 3:+102536212 None:intergenic
TCTACATGCACCTGAAATTC+TGG 0.295024 3:+102537191 None:intergenic
TAAATTACCTTTGTCCCATT+TGG 0.321779 3:+102536382 None:intergenic
TCAAGTCCATTATAAGCAAT+TGG 0.332783 3:+102536048 None:intergenic
TATGCCCCAATTGCTTATAA+TGG 0.346115 3:-102536054 MsaT016818.1:CDS
CTTCTGCTTGGAGAAAATAA+TGG 0.350452 3:-102537464 MsaT016818.1:CDS
CAAGTCCATTATAAGCAATT+GGG 0.382500 3:+102536049 None:intergenic
CTACAAAATCTTGTACCAAA+TGG 0.385534 3:-102536397 MsaT016818.1:CDS
CATTCCCATTCGCCATCATC+AGG 0.393250 3:+102537554 None:intergenic
ACCCTTGCATAGAGGCTTTG+AGG 0.393741 3:+102536678 None:intergenic
TACAAAATCTTGTACCAAAT+GGG 0.416760 3:-102536396 MsaT016818.1:CDS
GGAGAAAATAATGGGAACTT+TGG 0.422902 3:-102537455 MsaT016818.1:CDS
GAAGCCAATGCAAGTGTAGA+TGG 0.429354 3:-102536840 MsaT016818.1:CDS
TTCTGCTTGGAGAAAATAAT+GGG 0.435352 3:-102537463 MsaT016818.1:CDS
AACCTAAGTTGAAGCTGAAA+AGG 0.448825 3:-102537226 MsaT016818.1:CDS
ACATTGGATTCAATCTCAAC+CGG 0.458816 3:-102536026 MsaT016818.1:CDS
TTGCTCCCCTTCTGAAGCTG+AGG 0.486357 3:-102537417 MsaT016818.1:CDS
GATCAAACCTCGCTTCTGCT+TGG 0.495958 3:-102537476 MsaT016818.1:CDS
GCTTATAATGGACTTGACAT+TGG 0.507048 3:-102536042 MsaT016818.1:CDS
ATTTCTGCAGCTCCAAATCA+AGG 0.513225 3:-102536224 MsaT016818.1:intron
TATCCCTGATGATGGCGAAT+GGG 0.522217 3:-102537558 MsaT016818.1:CDS
ATGGTGGTGGAGAAATCTTC+CGG 0.539717 3:+102536007 None:intergenic
ATCCTCAAAGCCTCTATGCA+AGG 0.540493 3:-102536680 MsaT016818.1:CDS
ATGTTTCCCTCAGCTTCAGA+AGG 0.542520 3:+102537410 None:intergenic
CTTGGAGCTATCCCTGATGA+TGG 0.545545 3:-102537566 MsaT016818.1:CDS
TGCTCCCCTTCTGAAGCTGA+GGG 0.548801 3:-102537416 MsaT016818.1:CDS
GAAAGTAATCATTATACTCA+AGG 0.550308 3:-102537335 MsaT016818.1:CDS
AAGATAAGATAAACTCATGT+GGG 0.556515 3:-102537169 MsaT016818.1:CDS
ACTGTGTCTCTCAACTCCAA+TGG 0.558276 3:-102536735 MsaT016818.1:CDS
ATGTCCATCTACACTTGCAT+TGG 0.565311 3:+102536836 None:intergenic
TGATAATTACCCTTGCATAG+AGG 0.574539 3:+102536670 None:intergenic
TGTTTCCCTCAGCTTCAGAA+GGG 0.575825 3:+102537411 None:intergenic
CTATCCCTGATGATGGCGAA+TGG 0.588808 3:-102537559 MsaT016818.1:CDS
GAAGATAAGATAAACTCATG+TGG 0.590178 3:-102537170 MsaT016818.1:CDS
ATTGTGGTGGAGAGTCATCA+TGG 0.590276 3:+102537504 None:intergenic
TCCTCAAAGCCTCTATGCAA+GGG 0.593714 3:-102536679 MsaT016818.1:intron
TTCAGAAGGGGAGCAAAACA+TGG 0.599755 3:+102537424 None:intergenic
TCTTGTACCAAATGGGACAA+AGG 0.600952 3:-102536389 MsaT016818.1:intron
CTCTGAGGTATAACCACTAG+AGG 0.615332 3:+102536812 None:intergenic
AAGTCCATTATAAGCAATTG+GGG 0.617408 3:+102536050 None:intergenic
GTTTCCCTCAGCTTCAGAAG+GGG 0.626059 3:+102537412 None:intergenic
GGAAAGAAAAGAGCTAGTAG+AGG 0.626774 3:-102536714 MsaT016818.1:CDS
CTCTAGTGGTTATACCTCAG+AGG 0.631424 3:-102536811 MsaT016818.1:CDS
TTCTAAGCTCTGATGATCTG+TGG 0.632585 3:-102536080 MsaT016818.1:CDS
GAGGTTTGATCAAGTAATTG+TGG 0.634212 3:+102537488 None:intergenic
AGAGTCCAAGAAGAGCAAGA+AGG 0.635660 3:-102536889 MsaT016818.1:CDS
GTTTGATCAAGTAATTGTGG+TGG 0.639569 3:+102537491 None:intergenic
ATTCCCATTCGCCATCATCA+GGG 0.639610 3:+102537555 None:intergenic
GTAGATGGACATACCTCTAG+TGG 0.644629 3:-102536825 MsaT016818.1:CDS
CTTCAGTTGCAAATATTCTG+CGG 0.648235 3:+102537528 None:intergenic
GGTTGATAGAAATGAAAACA+AGG 0.650583 3:-102536868 MsaT016818.1:CDS
AGAGGAGCAAAACATGTGTG+AGG 0.671006 3:-102536793 MsaT016818.1:CDS
ATGTGTGAGGAAAACAGTGG+AGG 0.712518 3:-102536780 MsaT016818.1:CDS
AACATGTGTGAGGAAAACAG+TGG 0.786485 3:-102536783 MsaT016818.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 102536005 102537597 102536005 ID=MsaG016818
Chr3 mRNA 102536005 102537597 102536005 ID=MsaT016818.1;Parent=MsaG016818
Chr3 exon 102536005 102536103 102536005 ID=MsaT016818.1.exon5;Parent=MsaT016818.1
Chr3 CDS 102536005 102536103 102536005 ID=cds.MsaT016818.1;Parent=MsaT016818.1
Chr3 exon 102536225 102536290 102536225 ID=MsaT016818.1.exon4;Parent=MsaT016818.1
Chr3 CDS 102536225 102536290 102536225 ID=cds.MsaT016818.1;Parent=MsaT016818.1
Chr3 exon 102536390 102536455 102536390 ID=MsaT016818.1.exon3;Parent=MsaT016818.1
Chr3 CDS 102536390 102536455 102536390 ID=cds.MsaT016818.1;Parent=MsaT016818.1
Chr3 exon 102536680 102536934 102536680 ID=MsaT016818.1.exon2;Parent=MsaT016818.1
Chr3 CDS 102536680 102536934 102536680 ID=cds.MsaT016818.1;Parent=MsaT016818.1
Chr3 exon 102537136 102537597 102537136 ID=MsaT016818.1.exon1;Parent=MsaT016818.1
Chr3 CDS 102537136 102537597 102537136 ID=cds.MsaT016818.1;Parent=MsaT016818.1
Gene Sequence

>MsaT016818.1

ATGGAAACACTTGGAGCTATCCCTGATGATGGCGAATGGGAATGCTTCCGCAGAATATTTGCAACTGAAGACCATGATGACTCTCCACCACAATTACTTGATCAAACCTCGCTTCTGCTTGGAGAAAATAATGGGAACTTTGGAGTACAATCCATGTTTTGCTCCCCTTCTGAAGCTGAGGGAAACATGAATACTAATGTGTTTTATTCTTTTGATTCTCACATCAACTATAATTCACTAGAAAGTAATCATTATACTCAAGGTCACACAAACTACTATTTTGACTGTTCTGATCATGTGGTAGCTAATAATAGTGACAGAACAGAGAATTTTGATCACAATCAAGTCCAACCTAAGTTGAAGCTGAAAAGGATGCTTGATGTGCCAGAATTTCAGGTGCATGTAGAAGATAAGATAAACTCATGTGGGAACCCTAAGAAAAAACCTCGTGCTGCAAAAGATGTGCAAAGATGCATGAAAAATTCAGAGTCCAAGAAGAGCAAGAAGGTTGATAGAAATGAAAACAAGGCAGAAGAAGCCAATGCAAGTGTAGATGGACATACCTCTAGTGGTTATACCTCAGAGGAGCAAAACATGTGTGAGGAAAACAGTGGAGGAGCTTCTGCAGCATCTAAATCAACTGTGTCTCTCAACTCCAATGGAAAGAAAAGAGCTAGTAGAGGATCAGCAACAGATCCTCAAAGCCTCTATGCAAGGAAAAGAAGGGAGAAAATAAACGAGAGACTGAGAACTCTACAAAATCTTGTACCAAATGGGACAAAGGTTGATATAAGCACAATGCTTGAAGATGCAATCCAATATGTGAAATTTCTGCAGCTCCAAATCAAGCTTCTAAGCTCTGATGATCTGTGGATGTATGCCCCAATTGCTTATAATGGACTTGACATTGGATTCAATCTCAACCGGAAGATTTCTCCACCACCATGA

Protein sequence

>MsaT016818.1

METLGAIPDDGEWECFRRIFATEDHDDSPPQLLDQTSLLLGENNGNFGVQSMFCSPSEAEGNMNTNVFYSFDSHINYNSLESNHYTQGHTNYYFDCSDHVVANNSDRTENFDHNQVQPKLKLKRMLDVPEFQVHVEDKINSCGNPKKKPRAAKDVQRCMKNSESKKSKKVDRNENKAEEANASVDGHTSSGYTSEEQNMCEENSGGASAASKSTVSLNSNGKKRASRGSATDPQSLYARKRREKINERLRTLQNLVPNGTKVDISTMLEDAIQYVKFLQLQIKLLSSDDLWMYAPIAYNGLDIGFNLNRKISPPP*