AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016820


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016820 MtrunA17_Chr3g0134711 94.958 238 7 3 1 235 1 236 1.24e-150 418
MsaG016820 MtrunA17_Chr1g0152661 55.102 245 83 8 1 233 1 230 4.64e-78 234
MsaG016820 MtrunA17_Chr1g0207451 48.846 260 86 14 1 230 3 245 3.18e-56 179
MsaG016820 MtrunA17_Chr4g0012791 30.693 202 72 6 30 163 134 335 3.67e-19 85.9
MsaG016820 MtrunA17_Chr7g0231181 40.152 132 53 4 84 215 240 345 4.65e-19 84.7
MsaG016820 MtrunA17_Chr4g0012691 45.652 92 48 2 76 165 212 303 4.93e-19 84.7
MsaG016820 MtrunA17_Chr7g0263121 39.823 113 53 4 52 162 15 114 1.08e-18 80.5
MsaG016820 MtrunA17_Chr1g0182161 30.769 169 90 3 58 209 1 159 1.42e-18 80.1
MsaG016820 MtrunA17_Chr7g0222831 34.375 128 70 2 52 179 12 125 7.95e-17 75.9
MsaG016820 MtrunA17_Chr1g0197281 30.415 217 96 9 40 203 89 303 9.24e-17 78.2
MsaG016820 MtrunA17_Chr1g0180311 31.683 202 81 7 52 207 81 271 1.83e-16 76.6
MsaG016820 MtrunA17_Chr5g0448611 35.915 142 66 3 48 164 94 235 2.00e-16 76.3
MsaG016820 MtrunA17_Chr5g0425411 39.130 138 73 3 38 164 71 208 2.87e-16 75.5
MsaG016820 MtrunA17_Chr1g0212561 37.984 129 68 3 34 162 2 118 7.40e-16 73.6
MsaG016820 MtrunA17_Chr1g0182181 33.529 170 85 5 47 209 6 154 2.21e-15 71.2
MsaG016820 MtrunA17_Chr7g0263131 43.038 79 38 2 84 162 34 105 4.36e-15 70.5
MsaG016820 MtrunA17_Chr2g0327901 35.714 126 49 3 37 162 2 95 2.07e-14 67.8
MsaG016820 MtrunA17_Chr4g0061301 38.514 148 49 8 85 200 349 486 2.08e-14 72.0
MsaG016820 MtrunA17_Chr2g0320311 29.907 214 96 6 41 200 64 277 6.28e-14 69.7
MsaG016820 MtrunA17_Chr4g0056091 30.435 207 94 8 15 200 124 301 1.11e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016820 AT1G27730.1 51.899 237 96 7 1 231 1 225 3.69e-62 194
MsaG016820 AT5G04340.1 49.576 236 98 10 1 222 1 229 1.59e-55 177
MsaG016820 AT3G19580.1 45.802 262 102 10 1 227 1 257 4.94e-50 165
MsaG016820 AT3G19580.2 45.802 262 102 10 1 227 1 257 4.94e-50 165
MsaG016820 AT5G43170.1 48.718 234 70 11 1 226 1 192 5.57e-46 152
MsaG016820 AT3G49930.1 49.143 175 68 5 30 200 36 193 1.02e-39 136
MsaG016820 AT5G67450.1 41.250 240 98 8 1 200 1 237 1.81e-39 137
MsaG016820 AT2G45120.1 40.237 169 78 5 44 200 143 300 2.96e-24 98.6
MsaG016820 AT3G60580.1 37.209 172 79 4 44 200 119 276 8.99e-24 97.1
MsaG016820 AT2G28200.1 33.178 214 105 7 44 220 68 280 1.08e-22 94.0
MsaG016820 AT1G49900.1 51.579 95 40 2 85 178 194 283 8.81e-22 94.0
MsaG016820 AT1G49900.1 40.000 135 72 3 83 217 749 874 6.52e-18 82.8
MsaG016820 AT5G56200.1 47.423 97 38 2 84 167 342 438 1.46e-20 90.1
MsaG016820 AT5G03510.1 33.166 199 93 5 43 203 64 260 5.41e-19 84.0
MsaG016820 AT1G02040.1 31.771 192 94 8 5 163 61 248 1.72e-18 83.2
MsaG016820 AT2G37430.1 38.095 126 67 2 37 162 2 116 7.89e-17 75.9
MsaG016820 AT4G35280.1 38.400 125 53 4 48 164 138 246 1.23e-16 77.4
MsaG016820 AT1G02030.1 34.194 155 79 4 47 200 123 255 2.80e-16 76.3
MsaG016820 AT2G17180.1 30.625 160 76 5 48 200 121 252 7.93e-16 75.1
MsaG016820 AT3G46080.1 40.171 117 46 3 49 162 12 107 1.15e-15 72.4
MsaG016820 AT3G46090.1 36.923 130 62 3 39 167 3 113 1.82e-15 72.0
MsaG016820 AT5G59820.1 37.815 119 52 3 55 173 18 114 2.78e-15 71.2
MsaG016820 AT5G04390.1 28.889 225 90 7 48 203 96 319 6.36e-15 73.6
MsaG016820 AT3G53600.1 34.127 126 71 2 37 162 2 115 8.61e-15 70.5
MsaG016820 AT2G28710.1 39.640 111 43 3 52 162 14 100 5.43e-14 67.8
MsaG016820 AT3G10470.1 38.235 102 40 4 84 162 182 283 1.76e-12 66.6
MsaG016820 AT3G46070.1 34.545 110 55 3 53 162 16 108 2.28e-12 63.5
MsaG016820 AT5G61470.1 37.179 78 39 2 86 163 230 297 9.31e-11 60.8

Find 63 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTACTGGACAGGCTTT+GGG 0.284852 3:+102566579 MsaT016820.1:CDS
TTGCGGACATAACAGCTTTC+CGG 0.302034 3:-102566452 None:intergenic
CTTCCCTACTGGACAGGCTT+TGG 0.307779 3:+102566578 MsaT016820.1:CDS
GTTTCAAGATAACTCAGATT+TGG 0.369889 3:-102566195 None:intergenic
TACTCCTTTCACACCCTTTG+AGG 0.385893 3:+102566170 MsaT016820.1:CDS
GGCAGCGTTGGTGCCGGTGC+CGG 0.399502 3:+102566627 MsaT016820.1:CDS
CTCAGATTTGGTTCCTCAAA+GGG 0.424837 3:-102566183 None:intergenic
GTTGGTGCCGGTGCCGGTGC+TGG 0.425482 3:+102566633 MsaT016820.1:CDS
CATAACAGCTTTCCGGTGAC+TGG 0.439265 3:-102566445 None:intergenic
GGCTTCTTTGCAGCAGGTAA+AGG 0.446061 3:-102566783 None:intergenic
AATTTCCAGCTTGAAAAGAC+AGG 0.452679 3:-102566805 None:intergenic
CTGATCTTCAGCGGTGGTTG+CGG 0.460016 3:-102566469 None:intergenic
TTTGAAAAGTCCGGAAAAGC+CGG 0.466243 3:-102566729 None:intergenic
ACTGGCTTTGTGTCCACCTA+GGG 0.475209 3:-102566427 None:intergenic
GATCGTTTACCTTTCGTCCA+CGG 0.485045 3:-102566219 None:intergenic
CTTACCGTTGGAAGCAGAGC+TGG 0.488264 3:-102566520 None:intergenic
GCATTTGTGACTGAGTTTAA+CGG 0.491121 3:-102566370 None:intergenic
ATGAGTCTTGTTCTTACCGT+TGG 0.503655 3:-102566532 None:intergenic
TGTCACAAATCCTTCCCTAC+TGG 0.506283 3:+102566567 MsaT016820.1:CDS
AGACAGGGCTTCTTTGCAGC+AGG 0.516606 3:-102566789 None:intergenic
GGTTCCTCAAAGGGTGTGAA+AGG 0.517530 3:-102566174 None:intergenic
ACGATCAAAGCGTTCTCGCA+TGG 0.518878 3:+102566236 MsaT016820.1:CDS
TACGAAGGCAGCGTTGGTGC+CGG 0.519961 3:+102566621 MsaT016820.1:CDS
TTGAAAAGTCCGGAAAAGCC+GGG 0.523321 3:-102566728 None:intergenic
GACTGGCTTTGTGTCCACCT+AGG 0.526559 3:-102566428 None:intergenic
CAAATCCTTCCCTACTGGAC+AGG 0.527321 3:+102566572 MsaT016820.1:CDS
CTGAGTTTAACGGTGGTTAG+CGG 0.533899 3:-102566360 None:intergenic
CCTCCCAAAGCCTGTCCAGT+AGG 0.538116 3:-102566582 None:intergenic
ACTCAGATTTGGTTCCTCAA+AGG 0.539745 3:-102566184 None:intergenic
AGGGAAGGATTTGTGACAGA+TGG 0.550231 3:-102566562 None:intergenic
TTCTCATCTTATCAAGCCCT+AGG 0.550654 3:+102566411 MsaT016820.1:CDS
GTAACTGCCTCTGAAGGAGT+TGG 0.551176 3:+102566666 MsaT016820.1:CDS
CTCCCAAAGCCTGTCCAGTA+GGG 0.562622 3:-102566581 None:intergenic
CTTGAAACACCGTGGACGAA+AGG 0.563216 3:+102566210 MsaT016820.1:CDS
TGTGAAAGGAGTAGCAGTGG+TGG 0.569120 3:-102566160 None:intergenic
TGTCACTACGAAGGCAGCGT+TGG 0.571767 3:+102566615 MsaT016820.1:CDS
GGTAGTCACGGCGGATGAAG+TGG 0.575582 3:-102566499 None:intergenic
GCTACTTCCAGCACCGGCAC+CGG 0.578492 3:-102566640 None:intergenic
TAAGATCAAAATCACGGTGG+TGG 0.583762 3:-102566701 None:intergenic
CAAAGCCTGTCCAGTAGGGA+AGG 0.583815 3:-102566577 None:intergenic
GGAAGCAGAGCTGGTAGTCA+CGG 0.585255 3:-102566511 None:intergenic
CTCATCATGCTTGCTCGCAG+CGG 0.585296 3:+102566300 MsaT016820.1:CDS
GTGGACACAAAGCCAGTCAC+CGG 0.585980 3:+102566433 MsaT016820.1:CDS
ATTTCCAGCTTGAAAAGACA+GGG 0.591447 3:-102566804 None:intergenic
TACAGCGCTACTTCCAGCAC+CGG 0.591699 3:-102566646 None:intergenic
ATTATTGATCAGTAATGAGA+AGG 0.592327 3:-102566828 None:intergenic
GTGAAAGGAGTAGCAGTGGT+GGG 0.592634 3:-102566159 None:intergenic
AGCGCTGTAACTGCCTCTGA+AGG 0.607763 3:+102566660 MsaT016820.1:CDS
CCTACTGGACAGGCTTTGGG+AGG 0.612476 3:+102566582 MsaT016820.1:CDS
TCATCTTATCAAGCCCTAGG+TGG 0.625860 3:+102566414 MsaT016820.1:CDS
TGACGATCCAACTCCTTCAG+AGG 0.633382 3:-102566673 None:intergenic
GGGTGTGAAAGGAGTAGCAG+TGG 0.640901 3:-102566163 None:intergenic
ACTACCAGCTCTGCTTCCAA+CGG 0.645257 3:+102566516 MsaT016820.1:CDS
GGAGGTTAAGATCAAAATCA+CGG 0.658091 3:-102566707 None:intergenic
AGTCACGGCGGATGAAGTGG+TGG 0.674100 3:-102566496 None:intergenic
CACAAGCGTTGTCACTACGA+AGG 0.677003 3:+102566606 MsaT016820.1:CDS
TTTGTGACTGAGTTTAACGG+TGG 0.692042 3:-102566367 None:intergenic
AGCAGAGCTGGTAGTCACGG+CGG 0.700777 3:-102566508 None:intergenic
GGTGATCTGATCTTCAGCGG+TGG 0.700953 3:-102566475 None:intergenic
GGTGGTGATCTGATCTTCAG+CGG 0.704088 3:-102566478 None:intergenic
AAAAGTCCGGAAAAGCCGGG+AGG 0.720865 3:-102566725 None:intergenic
TGAGTTATCTTGAAACACCG+TGG 0.739154 3:+102566202 MsaT016820.1:CDS
GGTTAAGATCAAAATCACGG+TGG 0.807668 3:-102566704 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 102566135 102566842 102566135 ID=MsaG016820
Chr3 mRNA 102566135 102566842 102566135 ID=MsaT016820.1;Parent=MsaG016820
Chr3 exon 102566135 102566842 102566135 ID=MsaT016820.1.exon1;Parent=MsaT016820.1
Chr3 CDS 102566135 102566842 102566135 ID=cds.MsaT016820.1;Parent=MsaT016820.1
Gene Sequence

>MsaT016820.1

ATGGCTATGGAAGCACTTAACTCACCCACCACTGCTACTCCTTTCACACCCTTTGAGGAACCAAATCTGAGTTATCTTGAAACACCGTGGACGAAAGGTAAACGATCAAAGCGTTCTCGCATGGATCAATCTTCATGCACTGAAGAAGAGTATCTCGCTCTTTGTCTCATCATGCTTGCTCGCAGCGGTAACAACAACGACAAAAAGTCTGATTCGGTGGCGACGCCGCTAACCACCGTTAAACTCAGTCACAAATGCTCAGTCTGCAACAAAGCTTTCTCATCTTATCAAGCCCTAGGTGGACACAAAGCCAGTCACCGGAAAGCTGTTATGTCCGCAACCACCGCTGAAGATCAGATCACCACCACTTCATCCGCCGTGACTACCAGCTCTGCTTCCAACGGTAAGAACAAGACTCATGAGTGTTCCATCTGTCACAAATCCTTCCCTACTGGACAGGCTTTGGGAGGACACAAGCGTTGTCACTACGAAGGCAGCGTTGGTGCCGGTGCCGGTGCTGGAAGTAGCGCTGTAACTGCCTCTGAAGGAGTTGGATCGTCACACAGCCACCACCGTGATTTTGATCTTAACCTCCCGGCTTTTCCGGACTTTTCAAAGAAGTTTTTCGTGGATGACGAGGTTTCTAGTCCTTTACCTGCTGCAAAGAAGCCCTGTCTTTTCAAGCTGGAAATTCCTTCTCATTACTGA

Protein sequence

>MsaT016820.1

MAMEALNSPTTATPFTPFEEPNLSYLETPWTKGKRSKRSRMDQSSCTEEEYLALCLIMLARSGNNNDKKSDSVATPLTTVKLSHKCSVCNKAFSSYQALGGHKASHRKAVMSATTAEDQITTTSSAVTTSSASNGKNKTHECSICHKSFPTGQALGGHKRCHYEGSVGAGAGAGSSAVTASEGVGSSHSHHRDFDLNLPAFPDFSKKFFVDDEVSSPLPAAKKPCLFKLEIPSHY*