Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG016866 | PNX89510.1 | 40.132 | 152 | 66 | 3 | 133 | 259 | 4 | 155 | 2.73e-18 | 95.9 |
MsaG016866 | PNX89510.1 | 41.057 | 246 | 92 | 6 | 133 | 329 | 86 | 327 | 1.72e-34 | 139 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG016866 | tr|A0A2K3MFF0|A0A2K3MFF0_TRIPR | 40.132 | 152 | 66 | 3 | 133 | 259 | 4 | 155 | 1.31e-18 | 95.9 |
MsaG016866 | tr|A0A2K3MFF0|A0A2K3MFF0_TRIPR | 41.057 | 246 | 92 | 6 | 133 | 329 | 86 | 327 | 8.23e-35 | 139 |
Gene ID | Type | Classification |
---|---|---|
MsaG016866 | TF | C2H2 |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000135 | MsaG016866 | 0.833861 | 7.591880e-56 | 2.457889e-53 |
MsaG001730 | MsaG016866 | 0.845976 | 5.455732e-59 | 2.581817e-56 |
MsaG003422 | MsaG016866 | 0.864877 | 1.769762e-64 | 1.663821e-61 |
MsaG004500 | MsaG016866 | 0.807029 | 1.047526e-49 | 1.665595e-47 |
MsaG004621 | MsaG016866 | 0.833783 | 7.941425e-56 | 2.565274e-53 |
MsaG005100 | MsaG016866 | 0.815594 | 1.478613e-51 | 2.901591e-49 |
MsaG005496 | MsaG016866 | 0.821485 | 6.922257e-53 | 1.583482e-50 |
MsaG005497 | MsaG016866 | 0.836865 | 1.334078e-56 | 4.726026e-54 |
MsaG005722 | MsaG016866 | 0.824927 | 1.097653e-53 | 2.755748e-51 |
MsaG006555 | MsaG016866 | 0.830724 | 4.499770e-55 | 1.329468e-52 |
MsaG006657 | MsaG016866 | 0.806036 | 1.692736e-49 | 2.629165e-47 |
MsaG006765 | MsaG016866 | 0.810701 | 1.731562e-50 | 3.007907e-48 |
MsaG007442 | MsaG016866 | 0.825570 | 7.746247e-54 | 1.979414e-51 |
MsaG007630 | MsaG016866 | 0.820059 | 1.467506e-52 | 3.232697e-50 |
MsaG009389 | MsaG016866 | 0.814826 | 2.186133e-51 | 4.207524e-49 |
MsaG010706 | MsaG016866 | 0.824734 | 1.218336e-53 | 3.042479e-51 |
MsaG010853 | MsaG016866 | 0.814478 | 2.608613e-51 | 4.976679e-49 |
MsaG011244 | MsaG016866 | 0.815564 | 1.501431e-51 | 2.944092e-49 |
MsaG011217 | MsaG016866 | 0.842740 | 4.004012e-58 | 1.705034e-55 |
MsaG011873 | MsaG016866 | 0.827362 | 2.911455e-54 | 7.820563e-52 |
MsaG014075 | MsaG016866 | 0.815574 | 1.493267e-51 | 2.928874e-49 |
MsaG015445 | MsaG016866 | 0.803040 | 7.088330e-49 | 1.026938e-46 |
MsaG015485 | MsaG016866 | 0.836044 | 2.152918e-56 | 7.440555e-54 |
MsaG015829 | MsaG016866 | 0.824543 | 1.350786e-53 | 3.355831e-51 |
MsaG015832 | MsaG016866 | 0.829436 | 9.247786e-55 | 2.633490e-52 |
MsaG016215 | MsaG016866 | 0.812263 | 7.956098e-51 | 1.436066e-48 |
MsaG016273 | MsaG016866 | 0.817155 | 6.640969e-52 | 1.356308e-49 |
MsaG016397 | MsaG016866 | 0.823682 | 2.146451e-53 | 5.209317e-51 |
MsaG016517 | MsaG016866 | 0.849958 | 4.402447e-60 | 2.382669e-57 |
MsaG016818 | MsaG016866 | 0.811757 | 1.024287e-50 | 1.825949e-48 |
MsaG016866 | MsaG017219 | 0.828159 | 1.877203e-54 | 5.156378e-52 |
MsaG016866 | MsaG017495 | 0.867275 | 3.107439e-65 | 3.217834e-62 |
MsaG016866 | MsaG017700 | 0.816768 | 8.105154e-52 | 1.638881e-49 |
MsaG016866 | MsaG018598 | 0.831810 | 2.440917e-55 | 7.443485e-53 |
MsaG016866 | MsaG019237 | 0.841742 | 7.333596e-58 | 3.024626e-55 |
MsaG016866 | MsaG019944 | 0.808345 | 5.519632e-50 | 9.057185e-48 |
MsaG016866 | MsaG020215 | 0.840036 | 2.045746e-57 | 7.995457e-55 |
MsaG016866 | MsaG020492 | 0.818704 | 2.978468e-52 | 6.331042e-50 |
MsaG016866 | MsaG021720 | 0.806760 | 1.193524e-49 | 1.885798e-47 |
MsaG016866 | MsaG023047 | 0.805291 | 2.422448e-49 | 3.697445e-47 |
MsaG016866 | MsaG024295 | 0.829378 | 9.548849e-55 | 2.714688e-52 |
MsaG016866 | MsaG025740 | 0.841617 | 7.913185e-58 | 3.250756e-55 |
MsaG016866 | MsaG026692 | 0.806147 | 1.604817e-49 | 2.499121e-47 |
MsaG016866 | MsaG027376 | 0.829229 | 1.037367e-54 | 2.936351e-52 |
MsaG016866 | MsaG027919 | 0.800560 | 2.277104e-48 | 3.117949e-46 |
MsaG016866 | MsaG027988 | 0.835477 | 2.992241e-56 | 1.016731e-53 |
MsaG016866 | MsaG028679 | 0.803931 | 4.641942e-49 | 6.865231e-47 |
MsaG016866 | MsaG029063 | 0.804249 | 3.989995e-49 | 5.944698e-47 |
MsaG016866 | MsaG030039 | 0.823498 | 2.369480e-53 | 5.721645e-51 |
MsaG016866 | MsaG032373 | 0.807649 | 7.751125e-50 | 1.250771e-47 |
MsaG016866 | MsaG032915 | 0.804011 | 4.467841e-49 | 6.620177e-47 |
MsaG016866 | MsaG032947 | 0.807053 | 1.035386e-49 | 1.647220e-47 |
MsaG016866 | MsaG039178 | 0.802712 | 8.279152e-49 | 1.190410e-46 |
MsaG016866 | MsaG039487 | 0.812153 | 8.403521e-51 | 1.512692e-48 |
MsaG016866 | MsaG040269 | 0.827269 | 3.063689e-54 | 8.208160e-52 |
MsaG016866 | MsaG044107 | 0.817824 | 4.699748e-52 | 9.765360e-50 |
MsaG016866 | MsaG044436 | 0.800126 | 2.788011e-48 | 3.780184e-46 |
MsaG016866 | MsaG045331 | 0.810875 | 1.588651e-50 | 2.771388e-48 |
MsaG016866 | MsaG046376 | 0.801056 | 1.805494e-48 | 2.499917e-46 |
MsaG016866 | MsaG046497 | 0.827013 | 3.525401e-54 | 9.377482e-52 |
MsaG016866 | MsaG046660 | 0.818673 | 3.025612e-52 | 6.426143e-50 |
MsaG016866 | MsaG046730 | 0.825584 | 7.688833e-54 | 1.965459e-51 |
MsaG016866 | MsaG046972 | 0.856588 | 5.645129e-62 | 3.864053e-59 |
MsaG016866 | MsaG047069 | 0.832136 | 2.029095e-55 | 6.246592e-53 |
MsaG016866 | MsaG002316 | 0.836886 | 1.317205e-56 | 4.669304e-54 |
MsaG016866 | MsaG001966 | 0.816256 | 1.054040e-51 | 2.103499e-49 |
MsaG016866 | MsaG002000 | 0.818507 | 3.298386e-52 | 6.975372e-50 |
MsaG016866 | MsaG001807 | 0.823625 | 2.213062e-53 | 5.362675e-51 |
MsaG016866 | MsaG000634 | 0.817700 | 5.013042e-52 | 1.038232e-49 |
MsaG016866 | MsaG001987 | 0.847163 | 2.595765e-59 | 1.278110e-56 |
MsaG016866 | MsaG005167 | 0.806307 | 1.485265e-49 | 2.321850e-47 |
MsaG016866 | MsaG001893 | 0.864141 | 2.997963e-64 | 2.736997e-61 |
MsaG016866 | MsaG017934 | 0.802796 | 7.954955e-49 | 1.146023e-46 |
MsaG016866 | MsaG023445 | 0.837146 | 1.131283e-56 | 4.042059e-54 |
MsaG016866 | MsaG028734 | 0.823448 | 2.433204e-53 | 5.867558e-51 |
MsaG016866 | MsaG030607 | 0.824614 | 1.299893e-53 | 3.235536e-51 |
MsaG016866 | MsaG033319 | 0.840510 | 1.540754e-57 | 6.111698e-55 |
MsaG016866 | MsaG031847 | 0.812618 | 6.659647e-51 | 1.212727e-48 |
MsaG016866 | MsaG039696 | 0.855885 | 9.052158e-62 | 6.038458e-59 |
MsaG016866 | MsaG039788 | 0.818829 | 2.789899e-52 | 5.949707e-50 |
MsaG016866 | MsaG035600 | 0.811219 | 1.339026e-50 | 2.355774e-48 |
MsaG016866 | MsaG036651 | 0.832286 | 1.863904e-55 | 5.762637e-53 |
MsaG016866 | MsaG037725 | 0.817741 | 4.907247e-52 | 1.017410e-49 |
MsaG016866 | MsaG038853 | 0.812372 | 7.532109e-51 | 1.363273e-48 |
MsaG016866 | MsaG037508 | 0.800020 | 2.928273e-48 | 3.961099e-46 |
MsaG016866 | MsaG042585 | 0.831204 | 3.436185e-55 | 1.029329e-52 |
MsaG016866 | MsaG043047 | 0.802456 | 9.342050e-49 | 1.335457e-46 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 50 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TTTATGTCTTCACAAGCTTT+AGG | 0.218586 | 3:+103030787 | MsaT016866.1:CDS |
ATTTCTTGTTGATGCTTTGT+AGG | 0.248605 | 3:-103030844 | None:intergenic |
GATAATTCATCATTTCTTAA+AGG | 0.258078 | 3:-103029173 | None:intergenic |
ACATTCGACTTGTCTACTAT+TGG | 0.276222 | 3:-103030982 | None:intergenic |
CTTTCAAGCTTCTTCCTTGA+TGG | 0.289279 | 3:-103031034 | None:intergenic |
TTATGTCTTCACAAGCTTTA+GGG | 0.298843 | 3:+103030788 | MsaT016866.1:CDS |
TTATCATCAAATGGGTCATT+AGG | 0.302998 | 3:+103029191 | MsaT016866.1:CDS |
CTCACCGATGATTCGATTTA+CGG | 0.303465 | 3:+103030520 | MsaT016866.1:CDS |
GTTCTAATGTTGCATCTAAC+TGG | 0.314264 | 3:+103029273 | MsaT016866.1:CDS |
CATTCGACTTGTCTACTATT+GGG | 0.338002 | 3:-103030981 | None:intergenic |
AAACAAGTTCACTATCAAAA+TGG | 0.341580 | 3:+103029134 | MsaT016866.1:CDS |
AACGAAGAGATTGAAATTAG+AGG | 0.372610 | 3:+103030373 | MsaT016866.1:CDS |
ATCCTTAGGAGGACACAAAA+AGG | 0.374040 | 3:+103030627 | MsaT016866.1:CDS |
AATGATGAATTATCATCAAA+TGG | 0.381464 | 3:+103029182 | MsaT016866.1:CDS |
CGAATTATCGGTGAGATTGA+TGG | 0.404763 | 3:-103030686 | None:intergenic |
CATAGTAATCATCATGCATA+TGG | 0.409756 | 3:+103029302 | MsaT016866.1:CDS |
CGACCCACGAATCGAATTAT+CGG | 0.427366 | 3:-103030698 | None:intergenic |
AACAATACTACTAATATTGT+TGG | 0.430734 | 3:+103029248 | MsaT016866.1:CDS |
AAATACCTTGATGTTTCTGC+TGG | 0.436996 | 3:-103029363 | None:intergenic |
TCACCGATGATTCGATTTAC+GGG | 0.450292 | 3:+103030521 | MsaT016866.1:CDS |
CAAGCTTCTTCCTTGATGGT+AGG | 0.461171 | 3:-103031030 | None:intergenic |
GACGCAAGAAAATGTATTTG+AGG | 0.462564 | 3:-103030749 | None:intergenic |
ATGATGAATTATCATCAAAT+GGG | 0.500216 | 3:+103029183 | MsaT016866.1:CDS |
TCGCTTATCATCACTTGTTG+AGG | 0.515757 | 3:-103029218 | None:intergenic |
CGATAATTCGATTCGTGGGT+CGG | 0.519440 | 3:+103030699 | MsaT016866.1:CDS |
TACTCTTCACGATCCTTAGG+AGG | 0.520807 | 3:+103030616 | MsaT016866.1:CDS |
AATACTACTAATATTGTTGG+TGG | 0.531727 | 3:+103029251 | MsaT016866.1:CDS |
GTGAAGAGTAAAACTCTCTC+CGG | 0.531973 | 3:-103030603 | None:intergenic |
CTCACCGATAATTCGATTCG+TGG | 0.537516 | 3:+103030694 | MsaT016866.1:CDS |
TCGGTGAGATTAACGAAAAG+AGG | 0.543222 | 3:-103030505 | None:intergenic |
TAATTTCATCATAAGACCCT+CGG | 0.547601 | 3:-103030908 | None:intergenic |
AATTAGAGGATCAAATGCAA+GGG | 0.558949 | 3:+103030387 | MsaT016866.1:CDS |
TCGGTGAGATTGATGGAAAG+AGG | 0.566132 | 3:-103030679 | None:intergenic |
CGAGACCAGCAGAAACATCA+AGG | 0.569455 | 3:+103029358 | MsaT016866.1:CDS |
CTTATGATGAAATTAGAAGA+TGG | 0.574093 | 3:+103030916 | MsaT016866.1:CDS |
TATGTCTTCACAAGCTTTAG+GGG | 0.577249 | 3:+103030789 | MsaT016866.1:CDS |
ATGAAATTAGAAGATGGTGT+TGG | 0.580707 | 3:+103030922 | MsaT016866.1:CDS |
TCGAATGTGGTTGGCAGACG+TGG | 0.583035 | 3:+103030997 | MsaT016866.1:CDS |
GTAGACAAGTCGAATGTGGT+TGG | 0.590394 | 3:+103030988 | MsaT016866.1:CDS |
AAATTAGAGGATCAAATGCA+AGG | 0.596143 | 3:+103030386 | MsaT016866.1:CDS |
TGATCATCATCCTACCATCA+AGG | 0.614354 | 3:+103031020 | MsaT016866.1:CDS |
CGACCCGTAAATCGAATCAT+CGG | 0.620370 | 3:-103030524 | None:intergenic |
TCACCGATAATTCGATTCGT+GGG | 0.623824 | 3:+103030695 | MsaT016866.1:CDS |
ATGTCTTCACAAGCTTTAGG+GGG | 0.627825 | 3:+103030790 | MsaT016866.1:CDS |
CTTGCATGTAAAAGTCTCTG+AGG | 0.631561 | 3:-103030575 | None:intergenic |
ATTGATCGATTGAAGACCGA+GGG | 0.641525 | 3:+103030892 | MsaT016866.1:CDS |
AATAGTAGACAAGTCGAATG+TGG | 0.644495 | 3:+103030984 | MsaT016866.1:CDS |
ATTAGAGGATCAAATGCAAG+GGG | 0.648497 | 3:+103030388 | MsaT016866.1:CDS |
TATTTGTGTAGAAAACACGT+TGG | 0.679210 | 3:+103030350 | MsaT016866.1:intron |
TATTGATCGATTGAAGACCG+AGG | 0.701890 | 3:+103030891 | MsaT016866.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr3 | gene | 103029083 | 103031053 | 103029083 | ID=MsaG016866 |
Chr3 | mRNA | 103029083 | 103031053 | 103029083 | ID=MsaT016866.1;Parent=MsaG016866 |
Chr3 | exon | 103029083 | 103029379 | 103029083 | ID=MsaT016866.1.exon1;Parent=MsaT016866.1 |
Chr3 | CDS | 103029083 | 103029379 | 103029083 | ID=cds.MsaT016866.1;Parent=MsaT016866.1 |
Chr3 | exon | 103030361 | 103031053 | 103030361 | ID=MsaT016866.1.exon2;Parent=MsaT016866.1 |
Chr3 | CDS | 103030361 | 103031053 | 103030361 | ID=cds.MsaT016866.1;Parent=MsaT016866.1 |
Gene Sequence |
Protein sequence |