AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016866


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATGTCTTCACAAGCTTT+AGG 0.218586 3:+103030787 MsaT016866.1:CDS
ATTTCTTGTTGATGCTTTGT+AGG 0.248605 3:-103030844 None:intergenic
GATAATTCATCATTTCTTAA+AGG 0.258078 3:-103029173 None:intergenic
ACATTCGACTTGTCTACTAT+TGG 0.276222 3:-103030982 None:intergenic
CTTTCAAGCTTCTTCCTTGA+TGG 0.289279 3:-103031034 None:intergenic
TTATGTCTTCACAAGCTTTA+GGG 0.298843 3:+103030788 MsaT016866.1:CDS
TTATCATCAAATGGGTCATT+AGG 0.302998 3:+103029191 MsaT016866.1:CDS
CTCACCGATGATTCGATTTA+CGG 0.303465 3:+103030520 MsaT016866.1:CDS
GTTCTAATGTTGCATCTAAC+TGG 0.314264 3:+103029273 MsaT016866.1:CDS
CATTCGACTTGTCTACTATT+GGG 0.338002 3:-103030981 None:intergenic
AAACAAGTTCACTATCAAAA+TGG 0.341580 3:+103029134 MsaT016866.1:CDS
AACGAAGAGATTGAAATTAG+AGG 0.372610 3:+103030373 MsaT016866.1:CDS
ATCCTTAGGAGGACACAAAA+AGG 0.374040 3:+103030627 MsaT016866.1:CDS
AATGATGAATTATCATCAAA+TGG 0.381464 3:+103029182 MsaT016866.1:CDS
CGAATTATCGGTGAGATTGA+TGG 0.404763 3:-103030686 None:intergenic
CATAGTAATCATCATGCATA+TGG 0.409756 3:+103029302 MsaT016866.1:CDS
CGACCCACGAATCGAATTAT+CGG 0.427366 3:-103030698 None:intergenic
AACAATACTACTAATATTGT+TGG 0.430734 3:+103029248 MsaT016866.1:CDS
AAATACCTTGATGTTTCTGC+TGG 0.436996 3:-103029363 None:intergenic
TCACCGATGATTCGATTTAC+GGG 0.450292 3:+103030521 MsaT016866.1:CDS
CAAGCTTCTTCCTTGATGGT+AGG 0.461171 3:-103031030 None:intergenic
GACGCAAGAAAATGTATTTG+AGG 0.462564 3:-103030749 None:intergenic
ATGATGAATTATCATCAAAT+GGG 0.500216 3:+103029183 MsaT016866.1:CDS
TCGCTTATCATCACTTGTTG+AGG 0.515757 3:-103029218 None:intergenic
CGATAATTCGATTCGTGGGT+CGG 0.519440 3:+103030699 MsaT016866.1:CDS
TACTCTTCACGATCCTTAGG+AGG 0.520807 3:+103030616 MsaT016866.1:CDS
AATACTACTAATATTGTTGG+TGG 0.531727 3:+103029251 MsaT016866.1:CDS
GTGAAGAGTAAAACTCTCTC+CGG 0.531973 3:-103030603 None:intergenic
CTCACCGATAATTCGATTCG+TGG 0.537516 3:+103030694 MsaT016866.1:CDS
TCGGTGAGATTAACGAAAAG+AGG 0.543222 3:-103030505 None:intergenic
TAATTTCATCATAAGACCCT+CGG 0.547601 3:-103030908 None:intergenic
AATTAGAGGATCAAATGCAA+GGG 0.558949 3:+103030387 MsaT016866.1:CDS
TCGGTGAGATTGATGGAAAG+AGG 0.566132 3:-103030679 None:intergenic
CGAGACCAGCAGAAACATCA+AGG 0.569455 3:+103029358 MsaT016866.1:CDS
CTTATGATGAAATTAGAAGA+TGG 0.574093 3:+103030916 MsaT016866.1:CDS
TATGTCTTCACAAGCTTTAG+GGG 0.577249 3:+103030789 MsaT016866.1:CDS
ATGAAATTAGAAGATGGTGT+TGG 0.580707 3:+103030922 MsaT016866.1:CDS
TCGAATGTGGTTGGCAGACG+TGG 0.583035 3:+103030997 MsaT016866.1:CDS
GTAGACAAGTCGAATGTGGT+TGG 0.590394 3:+103030988 MsaT016866.1:CDS
AAATTAGAGGATCAAATGCA+AGG 0.596143 3:+103030386 MsaT016866.1:CDS
TGATCATCATCCTACCATCA+AGG 0.614354 3:+103031020 MsaT016866.1:CDS
CGACCCGTAAATCGAATCAT+CGG 0.620370 3:-103030524 None:intergenic
TCACCGATAATTCGATTCGT+GGG 0.623824 3:+103030695 MsaT016866.1:CDS
ATGTCTTCACAAGCTTTAGG+GGG 0.627825 3:+103030790 MsaT016866.1:CDS
CTTGCATGTAAAAGTCTCTG+AGG 0.631561 3:-103030575 None:intergenic
ATTGATCGATTGAAGACCGA+GGG 0.641525 3:+103030892 MsaT016866.1:CDS
AATAGTAGACAAGTCGAATG+TGG 0.644495 3:+103030984 MsaT016866.1:CDS
ATTAGAGGATCAAATGCAAG+GGG 0.648497 3:+103030388 MsaT016866.1:CDS
TATTTGTGTAGAAAACACGT+TGG 0.679210 3:+103030350 MsaT016866.1:intron
TATTGATCGATTGAAGACCG+AGG 0.701890 3:+103030891 MsaT016866.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 103029083 103031053 103029083 ID=MsaG016866
Chr3 mRNA 103029083 103031053 103029083 ID=MsaT016866.1;Parent=MsaG016866
Chr3 exon 103029083 103029379 103029083 ID=MsaT016866.1.exon1;Parent=MsaT016866.1
Chr3 CDS 103029083 103029379 103029083 ID=cds.MsaT016866.1;Parent=MsaT016866.1
Chr3 exon 103030361 103031053 103030361 ID=MsaT016866.1.exon2;Parent=MsaT016866.1
Chr3 CDS 103030361 103031053 103030361 ID=cds.MsaT016866.1;Parent=MsaT016866.1
Gene Sequence

>MsaT016866.1

ATGGTTTATCTTTTTGGAGTTAACATTGACTATCATCACAATCAACAACAAAAACAAGTTCACTATCAAAATGGTACGAGAAACAATGATCCTTTAAGAAATGATGAATTATCATCAAATGGGTCATTAGGATTTCCTCAACAAGTGATGATAAGCGACAACAACAACAATACTACTAATATTGTTGGTGGTTCTAATGTTGCATCTAACTGGTTTGTGCATAGTAATCATCATGCATATGGATCTCTATATTCTTATGTTAGAAACAACTCTCTCGAGACCAGCAGAAACATCAAGAAAACACGTTGGAACGAAGAGATTGAAATTAGAGGATCAAATGCAAGGGGAGAGACTTTTACATGTGCTTCTATTTCTGCTTGCAGCAACAACAACAACAAGCGAGTAAAAGTTGAACAATGTCAAATTTTAAACATCAAGACGCCTCTTTTCGTTAATCTCACCGATGATTCGATTTACGGGTCGAATATGCAAGAACGTGATTTTTTTGCGACCTCAGAGACTTTTACATGCAAGATTTGCCGGAGAGAGTTTTACTCTTCACGATCCTTAGGAGGACACAAAAAGGTTCATAAAGGTGAGAGCACACTCACAGAGCCTCTTTCCATCAATCTCACCGATAATTCGATTCGTGGGTCGGTGATGCAAGAAGATGATTTATACGCTACCTCAAATACATTTTCTTGCGTCATTTGTAAGAGAAAGTTTATGTCTTCACAAGCTTTAGGGGGACACAAAAAGGTTCATAAGGGTAAGCGTACACCTACAAAGCATCAACAAGAAATACAAGAGACGATAAATTTTTTGTCTATTGATCGATTGAAGACCGAGGGTCTTATGATGAAATTAGAAGATGGTGTTGGAGAGTCTTCTTCTATAAACGTTGATATGAAGTCAAACCCAATAGTAGACAAGTCGAATGTGGTTGGCAGACGTGGTGATCATCATCCTACCATCAAGGAAGAAGCTTGA

Protein sequence

>MsaT016866.1

MVYLFGVNIDYHHNQQQKQVHYQNGTRNNDPLRNDELSSNGSLGFPQQVMISDNNNNTTNIVGGSNVASNWFVHSNHHAYGSLYSYVRNNSLETSRNIKKTRWNEEIEIRGSNARGETFTCASISACSNNNNKRVKVEQCQILNIKTPLFVNLTDDSIYGSNMQERDFFATSETFTCKICRREFYSSRSLGGHKKVHKGESTLTEPLSINLTDNSIRGSVMQEDDLYATSNTFSCVICKRKFMSSQALGGHKKVHKGKRTPTKHQQEIQETINFLSIDRLKTEGLMMKLEDGVGESSSINVDMKSNPIVDKSNVVGRRGDHHPTIKEEA*