AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016882


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016882 MtrunA17_Chr3g0088371 47.674 172 73 6 128 292 23 184 2.98e-35 125
MsaG016882 MtrunA17_Chr1g0172971 82.692 52 9 0 1 52 66 117 1.90e-21 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCGATGAGTTAACTAATT+TGG 0.156761 3:-103266560 MsaT016882.1:CDS
TTAAGTCGCTTTGACCTAAT+TGG 0.232052 3:+103265431 None:intergenic
GAACACCGTTGAGAAATATT+TGG 0.241743 3:+103265601 None:intergenic
CGCTTTGACCTAATTGGATT+TGG 0.250918 3:+103265437 None:intergenic
AATCAGATTTCGACTACAAT+TGG 0.318540 3:-103266519 MsaT016882.1:CDS
CGTATGCATTGGTCTTGATT+TGG 0.322975 3:-103266422 MsaT016882.1:CDS
TTTGCTTCTTCCGAGCTATA+AGG 0.345757 3:+103265372 None:intergenic
TTCTCATACTCAACAAGAAC+TGG 0.395633 3:+103267817 None:intergenic
AAGAAAGACCAAATCCAATT+AGG 0.397771 3:-103265445 MsaT016882.1:CDS
TTTGGTCTTTCTTTGAACTT+TGG 0.398856 3:+103265455 None:intergenic
GTATGCATTGGTCTTGATTT+GGG 0.402784 3:-103266421 MsaT016882.1:CDS
GGAGAGTTTCTTATTTCTAA+AGG 0.407205 3:-103265560 MsaT016882.1:CDS
CATGTCGTATGTTTGAGAAC+CGG 0.411671 3:-103265873 MsaT016882.1:CDS
AGCTGGAATATATTTGAAGT+TGG 0.433136 3:-103267758 MsaT016882.1:CDS
CTCAACGGTGTTCTTGAGGC+GGG 0.436166 3:-103265591 MsaT016882.1:CDS
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 3:-103267775 MsaT016882.1:CDS
CGTTATGTTTGTAACAAAGA+AGG 0.449439 3:-103267892 MsaT016882.1:CDS
ATGTCGTATGTTTGAGAACC+GGG 0.461344 3:-103265872 MsaT016882.1:CDS
ACAAAATAAGAAATCGGATA+AGG 0.469178 3:-103267926 MsaT016882.1:CDS
AGGCCTACTTGGACTTTAAG+TGG 0.473126 3:-103266466 MsaT016882.1:CDS
GGGTTGTCACAGTCGACATT+GGG 0.480993 3:-103265915 MsaT016882.1:CDS
TTACAACCAACCCTTTCCTC+TGG 0.485330 3:+103267781 None:intergenic
CTGTCTGTGAACCATCTGTT+GGG 0.487793 3:-103265484 MsaT016882.1:CDS
CAATTCACAAAATAAGAAAT+CGG 0.490263 3:-103267932 MsaT016882.1:CDS
ATCAGATTTCGACTACAATT+GGG 0.491708 3:-103266518 MsaT016882.1:CDS
GCTGTCTGTGAACCATCTGT+TGG 0.504047 3:-103265485 MsaT016882.1:CDS
CTCATTCAAATCGTATGCAT+TGG 0.507227 3:-103266433 MsaT016882.1:CDS
ATCATAATCGACTTGATGAA+AGG 0.507955 3:-103265936 MsaT016882.1:CDS
TAACAAAGAAGGTTTCAAGA+AGG 0.525236 3:-103267881 MsaT016882.1:CDS
TCATAATCGACTTGATGAAA+GGG 0.526860 3:-103265935 MsaT016882.1:CDS
GACAGAAATTCCTTATAGCT+CGG 0.533906 3:-103265382 MsaT016882.1:CDS
CATTGGTCTTGATTTGGGTG+AGG 0.536683 3:-103266416 MsaT016882.1:CDS
AGGGTTGTCACAGTCGACAT+TGG 0.538587 3:-103265916 MsaT016882.1:CDS
AACAAAGAAGGTTTCAAGAA+GGG 0.545537 3:-103267880 MsaT016882.1:CDS
ATTTCTCAACGGTGTTCTTG+AGG 0.559987 3:-103265595 MsaT016882.1:CDS
TCAACGGTGTTCTTGAGGCG+GGG 0.572822 3:-103265590 MsaT016882.1:CDS
TTTGGTGCAGTCCCAACAGA+TGG 0.581566 3:+103265473 None:intergenic
GAAGCAAAAGAAAGATGATG+AGG 0.585686 3:-103265357 MsaT016882.1:CDS
TCTCAACGGTGTTCTTGAGG+CGG 0.585918 3:-103265592 MsaT016882.1:CDS
TGAAGACTTAGAAGGCGTAA+CGG 0.598405 3:+103265517 None:intergenic
AAGCCACTTAAAGTCCAAGT+AGG 0.617841 3:+103266463 None:intergenic
AGAACTGGACTGTCACATGT+TGG 0.620791 3:+103267832 None:intergenic
CATCGTGTTTGCAGATGCGG+AGG 0.621311 3:-103265627 MsaT016882.1:intron
GGCATCCAAATATTTCTCAA+CGG 0.643931 3:-103265606 MsaT016882.1:CDS
CATGTCGACATAAGAAACCC+CGG 0.663279 3:+103265854 None:intergenic
TGTCGTATGTTTGAGAACCG+GGG 0.673760 3:-103265871 MsaT016882.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 103265343 103267971 103265343 ID=MsaG016882
Chr3 mRNA 103265343 103267971 103265343 ID=MsaT016882.1;Parent=MsaG016882
Chr3 exon 103265343 103265635 103265343 ID=MsaT016882.1.exon4;Parent=MsaT016882.1
Chr3 CDS 103265343 103265635 103265343 ID=cds.MsaT016882.1;Parent=MsaT016882.1
Chr3 exon 103265830 103265966 103265830 ID=MsaT016882.1.exon3;Parent=MsaT016882.1
Chr3 CDS 103265830 103265966 103265830 ID=cds.MsaT016882.1;Parent=MsaT016882.1
Chr3 exon 103266379 103266588 103266379 ID=MsaT016882.1.exon2;Parent=MsaT016882.1
Chr3 CDS 103266379 103266588 103266379 ID=cds.MsaT016882.1;Parent=MsaT016882.1
Chr3 exon 103267727 103267971 103267727 ID=MsaT016882.1.exon1;Parent=MsaT016882.1
Chr3 CDS 103267727 103267971 103267727 ID=cds.MsaT016882.1;Parent=MsaT016882.1
Gene Sequence

>MsaT016882.1

ATGTTGAAGTCCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGTTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGATATATAATGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCGAATGATGAAAAAGCATTTTAATGGAAGGCCTACTTGGACTTTAAGTGGCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTGATTTGGGTGAGGTCACATTTTACTGGTGGTTTGAAAGCGACACGATTAAGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTGGGAGCAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGGTCTTAGATGCGGAGGCATCCAAATATTTCTCAACGGTGTTCTTGAGGCGGGGAAAAAGCGGAGAGTTTCTTATTTCTAAAGGTATTCATACAGATCGATCATCCGTTACGCCTTCTAAGTCTTCAAAAGCAGTTGCTGTCTGTGAACCATCTGTTGGGACTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAGGTCAAAGCGACTTAAAAGTGATTATGAAAAAGCTAGAGCAAGACAGAAATTCCTTATAGCTCGGAAGAAGCAAAAGAAAGATGATGAGGCTTTAACAAATTAA

Protein sequence

>MsaT016882.1

MLKSKNNSQNKKSDKVHYVRYVCNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKIYNAFFDELTNLVSNVEDESDFDYNWDRMMKKHFNGRPTWTLSGLFKLIQIVCIGLDLGEVTFYWWFESDTIKFEDHNRLDERVVTVDIGSKSFTCSCRMFENRGFLCRHVFKILEVLDAEASKYFSTVFLRRGKSGEFLISKGIHTDRSSVTPSKSSKAVAVCEPSVGTAPKFKERPNPIRSKRLKSDYEKARARQKFLIARKKQKKDDEALTN*