AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG016884


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016884 MtrunA17_Chr3g0134031 95.174 373 13 2 1 368 1 373 0.0 728
MsaG016884 MtrunA17_Chr5g0441531 46.450 338 86 12 1 331 1 250 2.40e-82 251
MsaG016884 MtrunA17_Chr5g0397881 45.652 230 107 6 1 224 1 218 4.02e-53 179
MsaG016884 MtrunA17_Chr6g0472421 44.643 224 89 6 15 223 24 227 1.09e-49 170
MsaG016884 MtrunA17_Chr1g0203601 40.930 215 94 3 16 225 10 196 8.46e-46 159
MsaG016884 MtrunA17_Chr5g0402291 42.986 221 85 6 17 225 2 193 1.98e-45 157
MsaG016884 MtrunA17_Chr7g0257331 38.034 234 91 6 9 233 2 190 5.17e-42 147
MsaG016884 MtrunA17_Chr6g0467041 69.892 93 24 1 23 111 21 113 1.29e-40 145
MsaG016884 MtrunA17_Chr8g0375041 38.426 216 83 5 17 225 2 174 2.69e-39 140
MsaG016884 MtrunA17_Chr5g0410881 68.421 95 26 1 21 111 29 123 8.32e-39 144
MsaG016884 MtrunA17_Chr7g0260331 65.306 98 30 1 18 111 43 140 8.78e-38 139
MsaG016884 MtrunA17_Chr8g0378061 68.132 91 25 1 25 111 23 113 4.87e-36 137
MsaG016884 MtrunA17_Chr1g0204741 62.136 103 35 1 18 116 34 136 6.08e-36 133
MsaG016884 MtrunA17_Chr8g0379701 60.606 99 34 2 17 111 6 103 6.47e-34 129
MsaG016884 MtrunA17_Chr4g0039891 40.415 193 63 5 24 212 12 156 1.94e-33 129
MsaG016884 MtrunA17_Chr1g0185131 60.194 103 36 2 22 119 30 132 9.23e-33 125
MsaG016884 MtrunA17_Chr4g0071051 55.455 110 44 2 18 122 95 204 4.07e-32 125
MsaG016884 MtrunA17_Chr6g0485571 57.895 95 36 1 21 111 8 102 2.82e-31 121
MsaG016884 MtrunA17_Chr3g0135831 53.153 111 43 2 18 119 40 150 5.09e-31 121
MsaG016884 MtrunA17_Chr2g0330821 61.702 94 32 1 22 111 77 170 1.55e-30 119
MsaG016884 MtrunA17_Chr2g0284481 57.000 100 38 2 17 112 9 107 1.83e-30 119
MsaG016884 MtrunA17_Chr4g0047421 55.000 100 37 2 21 112 30 129 1.50e-29 113
MsaG016884 MtrunA17_Chr8g0392001 58.511 94 34 2 23 111 21 114 1.44e-27 111
MsaG016884 MtrunA17_Chr1g0179321 65.574 61 21 0 24 84 49 109 2.18e-22 90.9
MsaG016884 MtrunA17_Chr4g0023771 52.632 76 35 1 17 91 8 83 1.68e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG016884 AT1G46264.1 54.430 395 104 15 1 367 1 347 2.38e-114 337
MsaG016884 AT4G11660.1 47.664 214 105 3 16 223 51 263 4.13e-54 183
MsaG016884 AT5G62020.1 43.460 237 108 5 17 249 16 230 4.15e-53 178
MsaG016884 AT4G36990.1 43.925 214 89 3 17 226 7 193 2.99e-48 165
MsaG016884 AT1G32330.2 41.667 204 54 4 21 212 34 184 3.41e-40 148
MsaG016884 AT1G32330.1 41.667 204 54 4 21 212 34 184 3.76e-40 148
MsaG016884 AT4G17750.1 66.667 99 29 1 20 114 48 146 1.47e-39 147
MsaG016884 AT2G41690.1 40.000 210 89 6 22 224 38 217 2.06e-39 140
MsaG016884 AT3G22830.1 63.000 100 33 1 18 113 55 154 1.57e-36 137
MsaG016884 AT5G03720.2 63.636 99 32 1 24 118 55 153 6.85e-35 132
MsaG016884 AT5G03720.1 63.636 99 32 1 24 118 55 153 6.85e-35 132
MsaG016884 AT3G02990.1 57.658 111 39 3 8 111 5 114 7.27e-35 134
MsaG016884 AT5G43840.1 61.386 101 35 1 22 118 17 117 2.92e-34 128
MsaG016884 AT5G16820.1 64.286 98 30 2 20 113 24 120 3.36e-34 132
MsaG016884 AT5G16820.2 64.286 98 30 2 20 113 24 120 3.36e-34 132
MsaG016884 AT2G26150.1 61.702 94 32 1 22 111 42 135 6.26e-33 126
MsaG016884 AT2G26150.4 61.702 94 32 1 22 111 42 135 6.26e-33 126
MsaG016884 AT3G24520.1 58.163 98 37 1 21 114 14 111 1.67e-32 124
MsaG016884 AT4G13980.1 62.637 91 30 1 25 111 24 114 3.07e-32 126
MsaG016884 AT3G51910.1 57.732 97 37 1 18 110 23 119 1.10e-31 120
MsaG016884 AT3G63350.1 56.701 97 38 1 18 110 22 118 3.54e-30 117
MsaG016884 AT5G54070.1 56.044 91 36 1 24 110 71 161 4.93e-29 115
MsaG016884 AT4G18880.1 60.606 99 34 2 17 111 9 106 3.78e-26 108
MsaG016884 AT1G67970.1 53.608 97 40 2 23 114 18 114 1.76e-25 106
MsaG016884 AT5G45710.3 55.435 92 37 1 24 111 13 104 6.77e-25 104
MsaG016884 AT5G45710.1 55.435 92 37 1 24 111 13 104 6.77e-25 104
MsaG016884 AT4G18870.1 39.362 94 50 2 18 104 137 230 5.75e-16 77.8
MsaG016884 AT4G18870.1 37.931 87 43 4 25 104 14 96 4.87e-12 66.2

Find 66 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTTGTTTCCAAACTATT+TGG 0.202751 3:-103289173 None:intergenic
AAACAAGGCTCGTTTGGTTT+TGG 0.245774 3:+103289190 MsaT016884.1:CDS
GGATTATTAGATCCACATTC+AGG 0.261808 3:-103289152 None:intergenic
TGAAGACGAGTCATATGTTT+AGG 0.266459 3:-103288978 None:intergenic
CTAACTCTAGTGTTTGAAAA+TGG 0.272288 3:-103288570 None:intergenic
AAGCCAATGAACCAGCTTTC+GGG 0.281382 3:+103288939 MsaT016884.1:CDS
ATCAACAAGTTGGTATGTTT+TGG 0.282286 3:-103288070 None:intergenic
TTAACTTCATCATTTGTTAA+TGG 0.296353 3:+103288654 MsaT016884.1:CDS
TTAACAAATGATGAAGTTAA+TGG 0.309297 3:-103288651 None:intergenic
TTGTTGTGTTTGAAGTAGTT+AGG 0.319993 3:-103288183 None:intergenic
ATCTAATAATCCAAATAGTT+TGG 0.322798 3:+103289163 MsaT016884.1:CDS
AGGAGGTCTCTAGCAAATTC+TGG 0.338598 3:-103288159 None:intergenic
ATGACTCCACCTTCGTTGTT+TGG 0.343318 3:+103288130 MsaT016884.1:CDS
TAGTTGTTTGTTGCTGTTGT+TGG 0.344004 3:-103288684 None:intergenic
AAAGCCAATGAACCAGCTTT+CGG 0.347409 3:+103288938 MsaT016884.1:CDS
TGTTGTGTTTGAAGTAGTTA+GGG 0.361431 3:-103288182 None:intergenic
CACAGATCACATCGTCTCTT+GGG 0.368339 3:+103288101 MsaT016884.1:CDS
GGAAACAAACAAGGCTCGTT+TGG 0.378669 3:+103289184 MsaT016884.1:CDS
GAGCTTCTTGATGTGTTTGT+TGG 0.386154 3:-103289041 None:intergenic
GAAGACGAGTCATATGTTTA+GGG 0.405370 3:-103288977 None:intergenic
TAAGAGTTACTTGGAGCTAC+TGG 0.420379 3:-103288840 None:intergenic
ACATTACCACCACCATTCAT+AGG 0.425778 3:-103288903 None:intergenic
TAGCCCGAAAGCTGGTTCAT+TGG 0.429834 3:-103288942 None:intergenic
CCACAGATCACATCGTCTCT+TGG 0.431871 3:+103288100 MsaT016884.1:CDS
TTGTTCATCAGAGTATTCAT+TGG 0.454805 3:-103288604 None:intergenic
CCACCACCATTCATAGGAGT+TGG 0.459985 3:-103288897 None:intergenic
CTGATGAACAAGTCAACAAC+TGG 0.462821 3:+103288616 MsaT016884.1:CDS
AGCTCAATAACAATGGTTGA+AGG 0.469701 3:+103289059 MsaT016884.1:CDS
ACATCACAGCCACAACAAAC+AGG 0.483395 3:+103288495 MsaT016884.1:CDS
GATGAAGAGTAAGAGTTACT+TGG 0.490480 3:-103288849 None:intergenic
TTAGCAAACTCCCATCTGTC+AGG 0.497806 3:-103288417 None:intergenic
GAAAATGGAAAGAAACTTGG+TGG 0.498648 3:-103288555 None:intergenic
GATTATTAGATCCACATTCA+GGG 0.505417 3:-103289151 None:intergenic
GTGGAACATTGTGATGCTGC+TGG 0.507886 3:-103288536 None:intergenic
TGTGTTTGAAGTAGTTAGGG+AGG 0.509850 3:-103288179 None:intergenic
TCATCAGAGTATTCATTGGA+TGG 0.511281 3:-103288600 None:intergenic
GAAAGATTGTACCTGACAGA+TGG 0.519136 3:+103288406 MsaT016884.1:intron
GTTTGAAGTAGTTAGGGAGG+AGG 0.523740 3:-103288176 None:intergenic
TCAAATCCAACTCCTATGAA+TGG 0.530340 3:+103288891 MsaT016884.1:CDS
AATTGTAATAGCCCGAAAGC+TGG 0.534218 3:-103288950 None:intergenic
TTTGAAAATGGAAAGAAACT+TGG 0.548152 3:-103288558 None:intergenic
TGGTGGTGGTAATGTTTCAA+TGG 0.548976 3:+103288911 MsaT016884.1:CDS
ACTTCATCATTTGTTAATGG+AGG 0.552014 3:+103288657 MsaT016884.1:CDS
CCACAAAATCAATCCCATCA+TGG 0.552202 3:+103289002 MsaT016884.1:CDS
AGTTCACATTCATACCATGA+TGG 0.552494 3:-103289016 None:intergenic
ATGTTGATTCCTGTTTGTTG+TGG 0.553850 3:-103288504 None:intergenic
AAAAGAGTGCACCCTGAATG+TGG 0.555827 3:+103289140 MsaT016884.1:CDS
ACAAATGATGAAGTTAATGG+TGG 0.561209 3:-103288648 None:intergenic
GTTCACATTCATACCATGAT+GGG 0.572426 3:-103289015 None:intergenic
CCAACTCCTATGAATGGTGG+TGG 0.572594 3:+103288897 MsaT016884.1:CDS
AAAGATTGTACCTGACAGAT+GGG 0.574158 3:+103288407 MsaT016884.1:intron
GCTCAATAACAATGGTTGAA+GGG 0.590591 3:+103289060 MsaT016884.1:CDS
GGAGTTGGATTTGAAGCTTG+TGG 0.599678 3:-103288882 None:intergenic
AAAACTAACTAACAAGTGGA+AGG 0.604739 3:-103289254 None:intergenic
TGGTTGGATCATCAACAAGT+TGG 0.622309 3:-103288080 None:intergenic
ACAGATCACATCGTCTCTTG+GGG 0.629510 3:+103288102 MsaT016884.1:CDS
ATCAAGAAGCTCAATAACAA+TGG 0.631659 3:+103289052 MsaT016884.1:CDS
GAGACGATGTGATCTGTGGT+TGG 0.633776 3:-103288096 None:intergenic
AAATAGTTTGGAAACAAACA+AGG 0.638741 3:+103289175 MsaT016884.1:CDS
AGGACGCCAAACAACGAAGG+TGG 0.642419 3:-103288136 None:intergenic
AATCCAACTCCTATGAATGG+TGG 0.654630 3:+103288894 MsaT016884.1:CDS
ACTAACTAACAAGTGGAAGG+AGG 0.655564 3:-103289251 None:intergenic
CTTCTACTAGACAACTGTGA+AGG 0.669455 3:+103287997 MsaT016884.1:CDS
AGGTCTCTAGCAAATTCTGG+AGG 0.693279 3:-103288156 None:intergenic
TGGAGGACGCCAAACAACGA+AGG 0.695849 3:-103288139 None:intergenic
CCAAGAGACGATGTGATCTG+TGG 0.707530 3:-103288100 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 103287991 103289268 103287991 ID=MsaG016884
Chr3 mRNA 103287991 103289268 103287991 ID=MsaT016884.1;Parent=MsaG016884
Chr3 exon 103287991 103288239 103287991 ID=MsaT016884.1.exon1;Parent=MsaT016884.1
Chr3 CDS 103287991 103288239 103287991 ID=cds.MsaT016884.1;Parent=MsaT016884.1
Chr3 exon 103288411 103289268 103288411 ID=MsaT016884.1.exon2;Parent=MsaT016884.1
Chr3 CDS 103288411 103289268 103288411 ID=cds.MsaT016884.1;Parent=MsaT016884.1
Gene Sequence

>MsaT016884.1

ATGGCTCTTCTACTAGACAACTGTGAAGGCATTTTACTCTCACTAGACTCACACAAATCAGTCCCAGCTCCATTCCTCACCAAAACATACCAACTTGTTGATGATCCAACCACAGATCACATCGTCTCTTGGGGTGAAGATGACTCCACCTTCGTTGTTTGGCGTCCTCCAGAATTTGCTAGAGACCTCCTCCCTAACTACTTCAAACACAACAACTTCTCTAGCTTCGTTCGACAACTCAACACCTATATTGTACCTGACAGATGGGAGTTTGCTAATGAATATTTCAAAAAAGGAGAAAAGCATTTGCTTTGTGAGATTCATAGAAGAAAAACATCACAGCCACAACAAACAGGAATCAACATGAATCATCACCAGCAGCATCACAATGTTCCACCAAGTTTCTTTCCATTTTCAAACACTAGAGTTAGCATTTCTCCATCCAATGAATACTCTGATGAACAAGTCAACAACTGGTGTGACTCTCCACCATTAACTTCATCATTTGTTAATGGAGGAACACCAACAACAGCAACAAACAACTATAACAGTTCTGTTACTGCTCTTTCTGAAGACAATGAAAGACTTAGAAGAAGTAACAACATGTTAATGTCTGAGCTAGCTCACATGAAGAAGCTTTACAATGATATTATCTACTTTGTTCAGAATCATGTTAAACCAGTAGCTCCAAGTAACTCTTACTCTTCATCTTTTCTACTTCCACAAGCTTCAAATCCAACTCCTATGAATGGTGGTGGTAATGTTTCAATGGTGCAAAAGCCAATGAACCAGCTTTCGGGCTATTACAATTCTAACCCTAAACATATGACTCGTCTTCAACCACAAAATCAATCCCATCATGGTATGAATGTGAACTCTCCAACAAACACATCAAGAAGCTCAATAACAATGGTTGAAGGGCTTAACAGTAATAACTGCAGCAAGACTAAGCTTTTTGGAGTATCACTTCAGTCCAAGAAAAGAGTGCACCCTGAATGTGGATCTAATAATCCAAATAGTTTGGAAACAAACAAGGCTCGTTTGGTTTTGGAAAAAGATCATGATTTTGATTTGGGTTTGAATCTCATGCCTCCTTCCACTTGTTAG

Protein sequence

>MsaT016884.1

MALLLDNCEGILLSLDSHKSVPAPFLTKTYQLVDDPTTDHIVSWGEDDSTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYIVPDRWEFANEYFKKGEKHLLCEIHRRKTSQPQQTGINMNHHQQHHNVPPSFFPFSNTRVSISPSNEYSDEQVNNWCDSPPLTSSFVNGGTPTTATNNYNSSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYSSSFLLPQASNPTPMNGGGNVSMVQKPMNQLSGYYNSNPKHMTRLQPQNQSHHGMNVNSPTNTSRSSITMVEGLNSNNCSKTKLFGVSLQSKKRVHPECGSNNPNSLETNKARLVLEKDHDFDLGLNLMPPSTC*