Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017021 | XP_024633844.1 | 88.102 | 353 | 23 | 4 | 1 | 341 | 1 | 346 | 0.0 | 608 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017021 | sp|Q9M2Y9|RAX3_ARATH | 55.607 | 214 | 71 | 5 | 1 | 200 | 1 | 204 | 4.69e-72 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017021 | tr|A0A072V235|A0A072V235_MEDTR | 88.102 | 353 | 23 | 4 | 1 | 341 | 1 | 346 | 0.0 | 608 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017021 | MtrunA17_Chr3g0135311 | 88.102 | 353 | 23 | 4 | 1 | 341 | 1 | 346 | 0.0 | 608 |
| MsaG017021 | MtrunA17_Chr1g0152011 | 53.757 | 346 | 110 | 14 | 1 | 312 | 1 | 330 | 2.23e-107 | 317 |
| MsaG017021 | MtrunA17_Chr5g0400821 | 65.193 | 181 | 38 | 5 | 1 | 168 | 1 | 169 | 3.07e-73 | 229 |
| MsaG017021 | MtrunA17_Chr4g0054391 | 66.316 | 190 | 38 | 6 | 1 | 177 | 1 | 177 | 3.70e-66 | 210 |
| MsaG017021 | MtrunA17_Chr4g0028311 | 78.151 | 119 | 16 | 1 | 1 | 109 | 1 | 119 | 8.75e-64 | 205 |
| MsaG017021 | MtrunA17_Chr4g0072751 | 82.883 | 111 | 9 | 1 | 1 | 101 | 1 | 111 | 1.50e-62 | 203 |
| MsaG017021 | MtrunA17_Chr7g0264641 | 65.248 | 141 | 38 | 2 | 1 | 130 | 1 | 141 | 6.02e-61 | 196 |
| MsaG017021 | MtrunA17_Chr2g0327351 | 78.378 | 111 | 14 | 1 | 1 | 101 | 1 | 111 | 3.78e-60 | 196 |
| MsaG017021 | MtrunA17_Chr1g0151941 | 72.881 | 118 | 22 | 1 | 1 | 108 | 1 | 118 | 8.94e-59 | 190 |
| MsaG017021 | MtrunA17_Chr6g0487491 | 52.747 | 182 | 52 | 4 | 1 | 164 | 1 | 166 | 2.72e-55 | 181 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017021 | AT3G49690.1 | 55.607 | 214 | 71 | 5 | 1 | 200 | 1 | 204 | 4.76e-73 | 229 |
| MsaG017021 | AT5G65790.1 | 78.788 | 132 | 17 | 2 | 1 | 122 | 1 | 131 | 2.07e-69 | 221 |
| MsaG017021 | AT5G57620.1 | 56.459 | 209 | 63 | 5 | 1 | 185 | 1 | 205 | 1.94e-68 | 218 |
| MsaG017021 | AT4G37780.1 | 51.869 | 214 | 73 | 5 | 1 | 184 | 1 | 214 | 1.45e-66 | 212 |
| MsaG017021 | AT2G36890.1 | 76.471 | 119 | 18 | 1 | 1 | 109 | 1 | 119 | 3.47e-61 | 198 |
| MsaG017021 | AT2G36890.2 | 76.471 | 119 | 18 | 1 | 1 | 109 | 42 | 160 | 5.49e-61 | 199 |
| MsaG017021 | AT5G23000.1 | 72.881 | 118 | 22 | 1 | 1 | 108 | 1 | 118 | 3.37e-58 | 191 |
| MsaG017021 | AT5G56110.1 | 66.102 | 118 | 31 | 1 | 1 | 109 | 1 | 118 | 1.23e-51 | 174 |
| MsaG017021 | AT5G16770.4 | 54.839 | 155 | 50 | 4 | 1 | 135 | 1 | 155 | 4.79e-50 | 170 |
| MsaG017021 | AT5G16770.3 | 54.839 | 155 | 50 | 4 | 1 | 135 | 1 | 155 | 4.79e-50 | 170 |
Find 69 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCACCATGCTTGATGTT+AGG | 0.346125 | 3:+104943370 | None:intergenic |
| AAATCCTACATCCAACAACA+TGG | 0.631031 | 3:-104943615 | MsaT017021.1:CDS |
| AAATTGGAAGCAACAAAAGG+AGG | 0.661204 | 3:+104942426 | None:intergenic |
| AAATTTCTAATCAAGCTTGG+AGG | 0.556795 | 3:-104942819 | MsaT017021.1:CDS |
| AACAATAATCAAGATGGGAA+GGG | 0.604958 | 3:-104943703 | None:intergenic |
| AACCACCATGCTTGATGTTA+GGG | 0.482233 | 3:+104943371 | None:intergenic |
| AACTAATCAATATTGTGTTG+AGG | 0.572203 | 3:-104942643 | MsaT017021.1:CDS |
| AAGTATAGTTATAGCTCCCT+AGG | 0.464728 | 3:+104942357 | None:intergenic |
| AATGATGTTGTCTTCTTCTT+CGG | 0.364573 | 3:+104943345 | None:intergenic |
| AATGTTAATCATGAGAGCAA+TGG | 0.599051 | 3:-104942981 | MsaT017021.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 104942365 | 104943712 | 104942365 | ID=MsaG017021 |
| Chr3 | mRNA | 104942365 | 104943712 | 104942365 | ID=MsaT017021.1;Parent=MsaG017021 |
| Chr3 | exon | 104942365 | 104943163 | 104942365 | ID=MsaT017021.1.exon3;Parent=MsaT017021.1 |
| Chr3 | CDS | 104942365 | 104943163 | 104942365 | ID=cds.MsaT017021.1;Parent=MsaT017021.1 |
| Chr3 | exon | 104943316 | 104943445 | 104943316 | ID=MsaT017021.1.exon2;Parent=MsaT017021.1 |
| Chr3 | CDS | 104943316 | 104943445 | 104943316 | ID=cds.MsaT017021.1;Parent=MsaT017021.1 |
| Chr3 | exon | 104943607 | 104943712 | 104943607 | ID=MsaT017021.1.exon1;Parent=MsaT017021.1 |
| Chr3 | CDS | 104943607 | 104943712 | 104943607 | ID=cds.MsaT017021.1;Parent=MsaT017021.1 |
| Gene Sequence |
>MsaT017021.1 ATGGGAAGGGCACCTTGCTGTGACAAAGCAAACGTGAAGAAAGGACCTTGGTCACCAGAAGAAGATGACAAGCTCAAATCCTACATCCAACAACATGGAACCGGTGGTCTTAAGCGATGTGGCAAGAGTTGTCGGCTGAGGTGGCTAAACTATCTTCGCCCTAACATCAAGCATGGTGGTTTCACCGAAGAAGAAGACAACATCATTTGCAGCCTTTATGTTACTATTGGAAGCAGATGGTCGGTGATTGCGGGCCAATTGCCGGGAAGAACCGATAATGATATAAAGAACTATTGGAACACAAAACTGAAAAAGAAGCTTTTAGGGAAGCATCGTAGAGAACAACAATTACAAGCACGTAGTAAATTATTAGCAAGTGCTAATCGTACTGTTGTTAATGTTAATCATGAGAGCAATGGAAGAAGGAGTGAGAATTCTTCATCTAATTTGGTTTCTGATCAACATATTTTACAGCCATATTGTTGGCCACAAAATATTCATCCATCGTTGACCTACACAAACCAAAGTTCTAGTTTCAACGATCAAGATTCTATTAGAAAATTTCTAATCAAGCTTGGAGGAAGATTCTCTGATGAAGATGATTATTATCAATTACATGATGGTGGTGTTCCTTCAAGACAACAAGTTCATGAAGAACAACAAGTCCATAATCTTTCTAGTTGCATCAACTCTATTAACAACAACCAAGTCTTGCAATATTTCTCACAAACTCAAACTAATCAATATTGTGTTGAGGGAGTAGCAGTACCAGAACTGGTACAAGTACAAGGAAATTTCACTGAAACGACAACATCTAGTAGTAATAATAATTATTATCCACAAAAGTTAAGTGGGTTGGAATTCATATATGGAGAGGACATTGATGGTACAATTTGTGGGCAAAGTAGTAATTGGGGTGAGACTGATCAGAGCAGTAGTAGTATGATATATCCTCCTTTTGTTGCTTCCAATTTCCAAGAAGAAAAGCCACAAGAATGTGTATTTCAAGAGTTGAGTTACCCTAGGGAGCTATAA |
| Protein sequence |
>MsaT017021.1 MGRAPCCDKANVKKGPWSPEEDDKLKSYIQQHGTGGLKRCGKSCRLRWLNYLRPNIKHGGFTEEEDNIICSLYVTIGSRWSVIAGQLPGRTDNDIKNYWNTKLKKKLLGKHRREQQLQARSKLLASANRTVVNVNHESNGRRSENSSSNLVSDQHILQPYCWPQNIHPSLTYTNQSSSFNDQDSIRKFLIKLGGRFSDEDDYYQLHDGGVPSRQQVHEEQQVHNLSSCINSINNNQVLQYFSQTQTNQYCVEGVAVPELVQVQGNFTETTTSSSNNNYYPQKLSGLEFIYGEDIDGTICGQSSNWGETDQSSSSMIYPPFVASNFQEEKPQECVFQELSYPREL* |
