AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017057


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017057 MtrunA17_Chr3g0135831 96.063 381 12 1 1 381 1 378 0.0 753
MsaG017057 MtrunA17_Chr7g0260331 54.924 264 112 2 8 264 8 271 6.12e-94 285
MsaG017057 MtrunA17_Chr1g0204741 62.963 216 73 4 36 246 27 240 9.73e-92 278
MsaG017057 MtrunA17_Chr5g0410881 55.357 224 84 4 36 247 21 240 1.99e-80 255
MsaG017057 MtrunA17_Chr4g0071051 40.431 371 177 11 24 376 76 420 6.44e-77 243
MsaG017057 MtrunA17_Chr4g0039891 54.808 208 86 2 48 247 11 218 3.23e-73 236
MsaG017057 MtrunA17_Chr1g0185131 44.224 303 143 8 36 331 19 302 3.68e-73 231
MsaG017057 MtrunA17_Chr6g0485571 45.274 201 106 2 49 249 11 207 6.18e-53 179
MsaG017057 MtrunA17_Chr8g0379701 40.517 232 130 2 48 275 11 238 4.90e-49 170
MsaG017057 MtrunA17_Chr8g0392001 45.876 194 98 2 49 235 22 215 5.43e-49 169
MsaG017057 MtrunA17_Chr8g0378061 43.284 201 104 5 50 247 23 216 5.54e-46 164
MsaG017057 MtrunA17_Chr2g0330821 40.933 193 110 1 43 235 73 261 5.71e-46 161
MsaG017057 MtrunA17_Chr6g0467041 42.365 203 107 5 49 248 22 217 6.72e-46 160
MsaG017057 MtrunA17_Chr2g0284481 41.243 177 101 1 48 221 14 190 2.00e-43 155
MsaG017057 MtrunA17_Chr5g0397881 62.766 94 35 0 47 140 22 115 5.56e-38 140
MsaG017057 MtrunA17_Chr7g0257331 45.062 162 76 3 40 188 9 170 1.76e-37 135
MsaG017057 MtrunA17_Chr3g0134031 56.757 111 43 1 43 153 18 123 8.26e-37 137
MsaG017057 MtrunA17_Chr5g0402291 55.882 102 45 0 46 147 6 107 8.93e-37 135
MsaG017057 MtrunA17_Chr6g0472421 58.511 94 39 0 47 140 31 124 9.08e-35 130
MsaG017057 MtrunA17_Chr1g0203601 54.206 107 49 0 47 153 16 122 1.28e-34 129
MsaG017057 MtrunA17_Chr1g0179311 38.537 205 120 4 109 310 14 215 4.45e-34 129
MsaG017057 MtrunA17_Chr8g0375041 52.727 110 50 1 44 153 4 111 5.25e-34 126
MsaG017057 MtrunA17_Chr5g0441531 60.638 94 37 0 47 140 23 116 6.83e-34 126
MsaG017057 MtrunA17_Chr4g0047421 36.957 184 101 5 28 204 17 192 4.78e-29 112
MsaG017057 MtrunA17_Chr1g0179321 63.514 74 27 0 35 108 35 108 3.02e-26 101
MsaG017057 MtrunA17_Chr4g0023771 51.429 70 33 1 40 108 6 75 6.52e-18 79.0
MsaG017057 MtrunA17_Chr2g0330841 32.051 156 97 4 96 247 26 176 1.71e-16 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017057 AT2G26150.1 51.862 349 124 7 36 379 31 340 2.57e-121 355
MsaG017057 AT2G26150.4 51.862 349 124 7 36 379 31 340 2.57e-121 355
MsaG017057 AT3G22830.1 44.258 357 158 8 36 359 48 396 5.94e-89 274
MsaG017057 AT3G51910.1 55.357 224 79 3 39 256 19 227 7.04e-77 239
MsaG017057 AT4G17750.1 51.570 223 91 2 47 252 50 272 3.11e-76 244
MsaG017057 AT1G32330.1 44.816 299 146 7 15 298 3 297 2.56e-75 241
MsaG017057 AT1G32330.2 44.816 299 146 7 15 298 3 297 3.19e-75 241
MsaG017057 AT5G16820.1 50.655 229 97 3 27 244 10 233 1.37e-74 239
MsaG017057 AT5G16820.2 50.655 229 97 3 27 244 10 233 1.37e-74 239
MsaG017057 AT3G02990.1 53.589 209 86 3 48 247 22 228 2.05e-73 236
MsaG017057 AT3G63350.1 43.492 315 118 7 25 331 4 266 1.80e-70 223
MsaG017057 AT5G43840.1 47.863 234 105 3 36 269 6 222 2.60e-69 219
MsaG017057 AT2G26150.3 44.404 277 108 8 109 379 49 285 5.76e-68 216
MsaG017057 AT2G26150.2 44.404 277 108 8 109 379 49 285 5.76e-68 216
MsaG017057 AT5G03720.2 45.815 227 111 4 17 240 24 241 1.09e-60 201
MsaG017057 AT5G03720.1 45.815 227 111 4 17 240 24 241 1.09e-60 201
MsaG017057 AT1G67970.1 47.872 188 97 1 49 235 19 206 5.74e-56 188
MsaG017057 AT5G54070.1 42.857 217 109 3 42 257 63 265 1.45e-55 186
MsaG017057 AT4G13980.1 40.201 199 114 2 50 247 24 218 3.98e-48 170
MsaG017057 AT3G24520.1 45.411 207 86 5 49 251 17 200 1.56e-47 165
MsaG017057 AT5G45710.3 38.725 204 118 2 48 251 12 208 2.92e-37 138
MsaG017057 AT5G45710.1 38.725 204 118 2 48 251 12 208 2.92e-37 138
MsaG017057 AT4G36990.1 52.632 114 48 1 47 160 12 119 1.53e-36 134
MsaG017057 AT1G46264.1 38.191 199 70 3 39 187 24 219 2.83e-36 135
MsaG017057 AT2G41690.1 48.889 135 52 2 6 140 14 131 5.48e-36 132
MsaG017057 AT4G18880.1 44.000 175 97 1 48 221 14 188 1.19e-35 135
MsaG017057 AT5G62020.1 55.882 102 45 0 47 148 21 122 1.79e-35 132
MsaG017057 AT4G11660.1 58.511 94 39 0 47 140 57 150 2.10e-34 131
MsaG017057 AT4G18870.1 34.711 121 75 1 49 165 146 266 1.31e-20 91.3
MsaG017057 AT4G18870.1 43.678 87 48 1 50 136 14 99 6.88e-18 83.6
MsaG017057 AT1G77570.1 36.667 90 54 2 50 136 27 116 6.34e-13 66.2

Find 81 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGCTTGTATTGAATT+AGG 0.198844 3:+105274781 MsaT017057.1:CDS
GAGAATTTGCAAGATGATTC+TGG 0.234265 3:+105275084 MsaT017057.1:CDS
CTCGTAATAGCTTTGTTGTT+TGG 0.240497 3:+105274256 MsaT017057.1:CDS
TGGTAGAAAATAATGATAAT+TGG 0.250597 3:+105275400 MsaT017057.1:CDS
AAGTGGTAATGAGAGTAATT+TGG 0.259305 3:+105275269 MsaT017057.1:CDS
TTCAACAACATCAAAGGTTT+TGG 0.271463 3:-105274196 None:intergenic
TGCAATCCTTCCATTGGTTT+TGG 0.278910 3:-105274147 None:intergenic
CCACTGATTCCATTGTTTCA+TGG 0.288131 3:+105274226 MsaT017057.1:CDS
TCGTAATAGCTTTGTTGTTT+GGG 0.292309 3:+105274257 MsaT017057.1:CDS
AACTCTTCATCTTCCTCTTC+GGG 0.307939 3:+105274111 MsaT017057.1:CDS
CCAATCACAACTTCATCTTC+TGG 0.312679 3:-105275342 None:intergenic
TGTATTGAATTAGGGGAGTA+TGG 0.320828 3:+105274790 MsaT017057.1:CDS
TAACTCTTCATCTTCCTCTT+CGG 0.322245 3:+105274110 MsaT017057.1:CDS
GCTCACTGCTAGTTCGAGTT+TGG 0.362423 3:+105275062 MsaT017057.1:CDS
TTAGCATATGTCAGTGTTGT+TGG 0.370710 3:+105274075 MsaT017057.1:CDS
TGATGTGCCCGTGGAGGATT+TGG 0.372993 3:+105275380 MsaT017057.1:CDS
GCAGAGCCTTGTTGATCAAA+TGG 0.381911 3:+105275434 MsaT017057.1:CDS
TGAATCAAGCAGTGAAGTTA+AGG 0.388539 3:+105275221 MsaT017057.1:CDS
AATGGAAGGATTGCACGAAA+TGG 0.400329 3:+105274155 MsaT017057.1:CDS
AGTGGTAATGAGAGTAATTT+GGG 0.400460 3:+105275270 MsaT017057.1:CDS
AAGATGATTCTGGAGCTATA+AGG 0.413178 3:+105275094 MsaT017057.1:CDS
GTGGTGCTTGTATTGAATTA+GGG 0.418246 3:+105274782 MsaT017057.1:CDS
TGGCTGAGATTGTGAAGTTA+AGG 0.421096 3:+105274860 MsaT017057.1:CDS
CAGAGCCTTGTTGATCAAAT+GGG 0.427250 3:+105275435 MsaT017057.1:CDS
AGATGATTCTGGAGCTATAA+GGG 0.444673 3:+105275095 MsaT017057.1:CDS
TTAGGGGAGTATGGTCTTGA+AGG 0.455498 3:+105274799 MsaT017057.1:CDS
GAAGGGAATTAGAGTGAAAG+AGG 0.460183 3:+105274044 MsaT017057.1:CDS
GTGAGATTGAGAGGTTGAGA+AGG 0.465030 3:+105274821 MsaT017057.1:CDS
GATTCCCACCAACCAACTCT+TGG 0.469023 3:-105275320 None:intergenic
ATGGAAGGATTGCACGAAAT+GGG 0.473051 3:+105274156 MsaT017057.1:CDS
CAAGCTATGCAGCAAGAGAC+TGG 0.480304 3:+105274760 MsaT017057.1:CDS
TAATCAACCACAGACTCAAT+TGG 0.480632 3:-105275123 None:intergenic
ATTTCGTGCAATCCTTCCAT+TGG 0.481224 3:-105274153 None:intergenic
GCGTTGAAATGAAGCGAAAA+CGG 0.487154 3:+105275037 MsaT017057.1:CDS
TGAGATTGAGAGGTTGAGAA+GGG 0.495911 3:+105274822 MsaT017057.1:CDS
AAGGGAGCAATTATCTGCTA+TGG 0.496135 3:+105274900 MsaT017057.1:CDS
GGCAGCAGCAGCATAATTCA+AGG 0.500409 3:+105274881 MsaT017057.1:CDS
CGATGGGAGTTTGCGAACGA+AGG 0.501252 3:+105274673 MsaT017057.1:CDS
TTGAGAGGTTGAGAAGGGAC+AGG 0.504469 3:+105274827 MsaT017057.1:CDS
TTCGCAAACTCCCATCGATC+CGG 0.510214 3:-105274667 None:intergenic
AATTATCTGCTATGGAAGCT+AGG 0.522811 3:+105274908 MsaT017057.1:CDS
CCAGAAGATGAAGTTGTGAT+TGG 0.531597 3:+105275342 MsaT017057.1:CDS
GACTACTATTGAGTCTGAGA+TGG 0.532873 3:+105275173 MsaT017057.1:CDS
CTGAACCAAGAGTTGGTTGG+TGG 0.536158 3:+105275315 MsaT017057.1:CDS
AAAAGTTGATCCGGATCGAT+GGG 0.538609 3:+105274657 MsaT017057.1:CDS
GAAAAGTTGATCCGGATCGA+TGG 0.539674 3:+105274656 MsaT017057.1:CDS
GATGGGAGTTTGCGAACGAA+GGG 0.539714 3:+105274674 MsaT017057.1:CDS
GGATTTACTGAACCAAGAGT+TGG 0.543897 3:+105275308 MsaT017057.1:CDS
TTTGGGTGATGCTGTGTGGG+AGG 0.547697 3:+105275287 MsaT017057.1:CDS
TAATTTGGGTGATGCTGTGT+GGG 0.557732 3:+105275284 MsaT017057.1:CDS
TGGATCTTCAACAACATCAA+AGG 0.559140 3:-105274202 None:intergenic
AAATTACTCTCATTACCACT+TGG 0.571022 3:-105275267 None:intergenic
AAATCTTACACCAAAACCAA+TGG 0.575232 3:+105274137 MsaT017057.1:CDS
GTCTTGAAGGTGAGATTGAG+AGG 0.580331 3:+105274812 MsaT017057.1:CDS
CTCTTGTCACGAGCAGCAAG+AGG 0.588881 3:+105275146 MsaT017057.1:CDS
CCATGAAACAATGGAATCAG+TGG 0.596228 3:-105274226 None:intergenic
GAAACAATGGAATCAGTGGA+TGG 0.602123 3:-105274222 None:intergenic
CAAAACCCATTTGATCAACA+AGG 0.605749 3:-105275440 None:intergenic
GCATCAACTAACTCAATGAA+GGG 0.610881 3:+105274027 None:intergenic
TGGTGCTTGTATTGAATTAG+GGG 0.613155 3:+105274783 MsaT017057.1:CDS
TGAGAGGTTGAGAAGGGACA+GGG 0.615785 3:+105274828 MsaT017057.1:CDS
TGAACCAAGAGTTGGTTGGT+GGG 0.615910 3:+105275316 MsaT017057.1:CDS
GATTCCATTGTTTCATGGAG+CGG 0.618876 3:+105274231 MsaT017057.1:CDS
AAATAATGATAATTGGACTG+TGG 0.619002 3:+105275407 MsaT017057.1:CDS
AACCACAGACTCAATTGGCA+CGG 0.620284 3:-105275118 None:intergenic
TGTAAGATTTGAACCCGAAG+AGG 0.623342 3:-105274124 None:intergenic
GCAGCAGCAGCATAATTCAA+GGG 0.624420 3:+105274882 MsaT017057.1:CDS
GGCCGTGCCAATTGAGTCTG+TGG 0.628717 3:+105275116 MsaT017057.1:CDS
TTCTCAAATTGATGTGCCCG+TGG 0.628790 3:+105275371 MsaT017057.1:CDS
GTAACGAGGTAAAATAGCAG+TGG 0.630092 3:-105274295 None:intergenic
AGCTCCGCTCCATGAAACAA+TGG 0.631104 3:-105274235 None:intergenic
CTTACACCAAAACCAATGGA+AGG 0.637289 3:+105274141 MsaT017057.1:CDS
TCAAATTGATGTGCCCGTGG+AGG 0.639975 3:+105275374 MsaT017057.1:CDS
TTACTGAACCAAGAGTTGGT+TGG 0.648299 3:+105275312 MsaT017057.1:CDS
TCTTGTCACGAGCAGCAAGA+GGG 0.651954 3:+105275147 MsaT017057.1:CDS
GCACAGAATAGAGAGTTGAA+AGG 0.656703 3:+105275015 MsaT017057.1:CDS
GTAATTTGGGTGATGCTGTG+TGG 0.674970 3:+105275283 MsaT017057.1:CDS
TTGAAATGAAGCGAAAACGG+AGG 0.685238 3:+105275040 MsaT017057.1:CDS
GCATTGAGTTCAGTTCCAAG+TGG 0.687922 3:+105275252 MsaT017057.1:CDS
TTACTGTGTTTGAAGTAACG+AGG 0.690403 3:-105274309 None:intergenic
GCTATGCAGCAAGAGACTGG+TGG 0.743962 3:+105274763 MsaT017057.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 105274042 105275470 105274042 ID=MsaG017057
Chr3 mRNA 105274042 105275470 105274042 ID=MsaT017057.1;Parent=MsaG017057
Chr3 exon 105274042 105274365 105274042 ID=MsaT017057.1.exon1;Parent=MsaT017057.1
Chr3 CDS 105274042 105274365 105274042 ID=cds.MsaT017057.1;Parent=MsaT017057.1
Chr3 exon 105274649 105275470 105274649 ID=MsaT017057.1.exon2;Parent=MsaT017057.1
Chr3 CDS 105274649 105275470 105274649 ID=cds.MsaT017057.1;Parent=MsaT017057.1
Gene Sequence

>MsaT017057.1

ATGAAGGGAATTAGAGTGAAAGAGGAAGAAACATTAGCATATGTCAGTGTTGTTGGTTCAACATCATGTAACTCTTCATCTTCCTCTTCGGGTTCAAATCTTACACCAAAACCAATGGAAGGATTGCACGAAATGGGTCCACCTCCATTTCTCACCAAAACCTTTGATGTTGTTGAAGATCCATCCACTGATTCCATTGTTTCATGGAGCGGAGCTCGTAATAGCTTTGTTGTTTGGGATCTTCACAAATTCTCCACTGCTATTTTACCTCGTTACTTCAAACACAGTAATTTCTCTAGCTTCGTTCGACAACTCAATACCTATGGTTTTAGAAAAGTTGATCCGGATCGATGGGAGTTTGCGAACGAAGGGTTTTTAGCTGGGCAGAGGAACTTGTTGAAAACAATCAAGAGAAGAAGAAATGTGACACAATCACAAGCTATGCAGCAAGAGACTGGTGGTGCTTGTATTGAATTAGGGGAGTATGGTCTTGAAGGTGAGATTGAGAGGTTGAGAAGGGACAGGGCTGTTTTAATGGCTGAGATTGTGAAGTTAAGGCAGCAGCAGCATAATTCAAGGGAGCAATTATCTGCTATGGAAGCTAGGTTGTTGATTACTGAGAAGAAACATCAACAGATGATGAATTTTCTTGCAAAAGCACTTAGCAATCAATCTTTTATACAACAATTGGCACAGAATAGAGAGTTGAAAGGCGTTGAAATGAAGCGAAAACGGAGGCTCACTGCTAGTTCGAGTTTGGAGAATTTGCAAGATGATTCTGGAGCTATAAGGGCCGTGCCAATTGAGTCTGTGGTTGATTACTCTTGTCACGAGCAGCAAGAGGGTTTGACTACTATTGAGTCTGAGATGGAGACACTGTTAAGTGCTTATGATAATGAATCAAGCAGTGAAGTTAAGGACTACAGTGCATTGAGTTCAGTTCCAAGTGGTAATGAGAGTAATTTGGGTGATGCTGTGTGGGAGGATTTACTGAACCAAGAGTTGGTTGGTGGGAATCCAGAAGATGAAGTTGTGATTGGTGATTTTTCTCAAATTGATGTGCCCGTGGAGGATTTGGTAGAAAATAATGATAATTGGACTGTGGATTTGCAGAGCCTTGTTGATCAAATGGGTTTTGAACCTTAG

Protein sequence

>MsaT017057.1

MKGIRVKEEETLAYVSVVGSTSCNSSSSSSGSNLTPKPMEGLHEMGPPPFLTKTFDVVEDPSTDSIVSWSGARNSFVVWDLHKFSTAILPRYFKHSNFSSFVRQLNTYGFRKVDPDRWEFANEGFLAGQRNLLKTIKRRRNVTQSQAMQQETGGACIELGEYGLEGEIERLRRDRAVLMAEIVKLRQQQHNSREQLSAMEARLLITEKKHQQMMNFLAKALSNQSFIQQLAQNRELKGVEMKRKRRLTASSSLENLQDDSGAIRAVPIESVVDYSCHEQQEGLTTIESEMETLLSAYDNESSSEVKDYSALSSVPSGNESNLGDAVWEDLLNQELVGGNPEDEVVIGDFSQIDVPVEDLVENNDNWTVDLQSLVDQMGFEP*