Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017068 | XP_003603174.1 | 98.826 | 511 | 4 | 2 | 1 | 511 | 1 | 509 | 0.0 | 1050 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017068 | sp|Q6TGC6|CBK1_PNECA | 46.288 | 458 | 234 | 4 | 23 | 475 | 53 | 503 | 1.06e-144 | 428 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017068 | tr|G7J8B8|G7J8B8_MEDTR | 98.826 | 511 | 4 | 2 | 1 | 511 | 1 | 509 | 0.0 | 1050 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000033 | MsaG017068 | 0.835122 | 3.674732e-56 | 1.235395e-53 |
| MsaG000125 | MsaG017068 | 0.823527 | 2.332595e-53 | 5.637072e-51 |
| MsaG000412 | MsaG017068 | 0.836208 | 1.957255e-56 | 6.797933e-54 |
| MsaG000717 | MsaG017068 | 0.907379 | 1.287571e-80 | 1.028929e-76 |
| MsaG000788 | MsaG017068 | 0.839898 | 2.221197e-57 | 8.643785e-55 |
| MsaG001363 | MsaG017068 | 0.830352 | 5.545115e-55 | 1.621025e-52 |
| MsaG001468 | MsaG017068 | 0.860015 | 5.446181e-63 | 4.236660e-60 |
| MsaG001551 | MsaG017068 | 0.828183 | 1.852935e-54 | 5.093227e-52 |
| MsaG001730 | MsaG017068 | 0.896403 | 8.631218e-76 | 3.592732e-72 |
| MsaG001939 | MsaG017068 | 0.850856 | 2.471698e-60 | 1.379717e-57 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017068 | MtrunA17_Chr3g0135951 | 98.826 | 511 | 4 | 2 | 1 | 511 | 1 | 509 | 0.0 | 1050 |
| MsaG017068 | MtrunA17_Chr2g0299781 | 77.660 | 470 | 104 | 1 | 12 | 481 | 29 | 497 | 0.0 | 773 |
| MsaG017068 | MtrunA17_Chr1g0183651 | 68.640 | 456 | 133 | 2 | 20 | 475 | 44 | 489 | 0.0 | 661 |
| MsaG017068 | MtrunA17_Chr1g0187581 | 68.202 | 456 | 136 | 3 | 20 | 475 | 41 | 487 | 0.0 | 648 |
| MsaG017068 | MtrunA17_Chr7g0261491 | 64.570 | 477 | 156 | 2 | 20 | 492 | 44 | 511 | 0.0 | 647 |
| MsaG017068 | MtrunA17_Chr1g0167351 | 65.678 | 472 | 148 | 5 | 15 | 483 | 29 | 489 | 0.0 | 628 |
| MsaG017068 | MtrunA17_Chr1g0153321 | 64.392 | 469 | 158 | 3 | 6 | 474 | 18 | 477 | 0.0 | 627 |
| MsaG017068 | MtrunA17_Chr1g0167311 | 63.077 | 455 | 157 | 5 | 20 | 473 | 39 | 483 | 0.0 | 584 |
| MsaG017068 | MtrunA17_Chr6g0478601 | 41.926 | 353 | 183 | 8 | 81 | 431 | 96 | 428 | 4.10e-83 | 267 |
| MsaG017068 | MtrunA17_Chr5g0428981 | 40.286 | 350 | 183 | 9 | 86 | 432 | 753 | 1079 | 1.78e-74 | 255 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017068 | AT4G33080.2 | 78.865 | 511 | 102 | 4 | 1 | 510 | 1 | 506 | 0.0 | 838 |
| MsaG017068 | AT4G33080.1 | 76.775 | 521 | 107 | 4 | 1 | 510 | 1 | 518 | 0.0 | 830 |
| MsaG017068 | AT2G19400.1 | 74.255 | 470 | 118 | 2 | 14 | 481 | 23 | 491 | 0.0 | 733 |
| MsaG017068 | AT4G14350.2 | 68.220 | 472 | 136 | 5 | 6 | 475 | 30 | 489 | 0.0 | 655 |
| MsaG017068 | AT4G14350.1 | 68.220 | 472 | 136 | 5 | 6 | 475 | 30 | 489 | 0.0 | 655 |
| MsaG017068 | AT4G14350.4 | 68.220 | 472 | 136 | 5 | 6 | 475 | 30 | 489 | 0.0 | 655 |
| MsaG017068 | AT4G14350.3 | 68.220 | 472 | 136 | 5 | 6 | 475 | 30 | 489 | 0.0 | 655 |
| MsaG017068 | AT3G23310.1 | 67.516 | 471 | 144 | 5 | 3 | 473 | 29 | 490 | 0.0 | 654 |
| MsaG017068 | AT2G20470.3 | 66.447 | 456 | 142 | 3 | 20 | 475 | 48 | 492 | 0.0 | 639 |
| MsaG017068 | AT1G03920.1 | 66.595 | 464 | 144 | 4 | 12 | 475 | 54 | 506 | 0.0 | 639 |
Find 128 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGAACAGCCGATGAAAC+AGG | 0.429673 | 3:+105431004 | MsaT017068.1:CDS |
| AAACTTTATGAAGTTTGATG+AGG | 0.571370 | 3:+105428611 | MsaT017068.1:CDS |
| AAATCTGAAATGCTCAGGAG+AGG | 0.649300 | 3:+105425783 | MsaT017068.1:CDS |
| AAATGGAGGAGGAGAAGAAG+AGG | 0.500875 | 3:+105423733 | MsaT017068.1:CDS |
| AACACAGCTATCGATATCCA+TGG | 0.554546 | 3:-105426561 | None:intergenic |
| AACTTCGATGCTGTTAAAGA+AGG | 0.527196 | 3:+105430545 | MsaT017068.1:CDS |
| AAGTTTCACAATGCAATGAC+TGG | 0.563826 | 3:-105426091 | None:intergenic |
| AATGCAATGACTGGCGACTT+CGG | 0.495853 | 3:-105426082 | None:intergenic |
| AATGCTGAAGCTGCTCACGC+GGG | 0.549920 | 3:-105426616 | None:intergenic |
| AATTACAGGTTAGACTGTGT+CGG | 0.542157 | 3:+105425718 | MsaT017068.1:intron |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 105423707 | 105431069 | 105423707 | ID=MsaG017068 |
| Chr3 | mRNA | 105423707 | 105431069 | 105423707 | ID=MsaT017068.1;Parent=MsaG017068 |
| Chr3 | exon | 105423707 | 105423858 | 105423707 | ID=MsaT017068.1.exon1;Parent=MsaT017068.1 |
| Chr3 | CDS | 105423707 | 105423858 | 105423707 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105425384 | 105425558 | 105425384 | ID=MsaT017068.1.exon2;Parent=MsaT017068.1 |
| Chr3 | CDS | 105425384 | 105425558 | 105425384 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105425726 | 105425809 | 105425726 | ID=MsaT017068.1.exon3;Parent=MsaT017068.1 |
| Chr3 | CDS | 105425726 | 105425809 | 105425726 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105426048 | 105426289 | 105426048 | ID=MsaT017068.1.exon4;Parent=MsaT017068.1 |
| Chr3 | CDS | 105426048 | 105426289 | 105426048 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105426418 | 105426661 | 105426418 | ID=MsaT017068.1.exon5;Parent=MsaT017068.1 |
| Chr3 | CDS | 105426418 | 105426661 | 105426418 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105427421 | 105427500 | 105427421 | ID=MsaT017068.1.exon6;Parent=MsaT017068.1 |
| Chr3 | CDS | 105427421 | 105427500 | 105427421 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105427764 | 105427848 | 105427764 | ID=MsaT017068.1.exon7;Parent=MsaT017068.1 |
| Chr3 | CDS | 105427764 | 105427848 | 105427764 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105428064 | 105428195 | 105428064 | ID=MsaT017068.1.exon8;Parent=MsaT017068.1 |
| Chr3 | CDS | 105428064 | 105428195 | 105428064 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105428519 | 105428632 | 105428519 | ID=MsaT017068.1.exon9;Parent=MsaT017068.1 |
| Chr3 | CDS | 105428519 | 105428632 | 105428519 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105429298 | 105429345 | 105429298 | ID=MsaT017068.1.exon10;Parent=MsaT017068.1 |
| Chr3 | CDS | 105429298 | 105429345 | 105429298 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105430497 | 105430584 | 105430497 | ID=MsaT017068.1.exon11;Parent=MsaT017068.1 |
| Chr3 | CDS | 105430497 | 105430584 | 105430497 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Chr3 | exon | 105430978 | 105431069 | 105430978 | ID=MsaT017068.1.exon12;Parent=MsaT017068.1 |
| Chr3 | CDS | 105430978 | 105431069 | 105430978 | ID=cds.MsaT017068.1;Parent=MsaT017068.1 |
| Gene Sequence |
>MsaT017068.1 ATGGACGATCTTCAGGAGGAGGGAGAAAATGGAGGAGGAGAAGAAGAGGTGCTTGGTTCAAGCTTGACTATGGAGAAAGTAGCAGCTGCTAAGAAATTTATTGAGAATCATTACAGGGCACAGATGAAAAATATTCAAGATCGGAAAGAGAGGCGTTGGGTATTGGAAAGAAAATTAGCTACTTCGGATGTGCCAACTGAAGAACGGCTCAACCTCATCAAAGATTTAGAGAGGAAGGAAACTGAATATATGCGATTGAAAAGACATAAGATTTGTGTTAATGATTTTGATCTCTTGACCATCATTGGAAGGGGGGCTTTTGGGGAGGTTAGACTGTGTCGGGAAAAGAAATCTGGAAATATCTATGCCATGAAAAAGCTGAAGAAATCTGAAATGCTCAGGAGAGGCCAGGTAGAACATGTTAGAGCTGAGAGGAACTTACTGGCCGAAGTCGCCAGTCATTGCATTGTGAAACTTTACTACTCATTTCAAGATACTGAATACTTGTATTTGATTATGGAGTATCTGCCTGGGGGTGACATCATGACTCTGCTTATGAGGGAAGACACGTTAAGTGAGAACGTGGCTAGATTTTACATGGCACAGAGTGTTCTGGCCATAGAGTCTATTCATAAGCACAATTACATTCACAGAGATATTAAACCCGATAACTTACTTCTGGATAAAAATGGTCATATGAAGCTCTCTGATTTTGGCCTCTGTAAGCCTATCGATTGCATTACCTTGCCTACACTGCATGAAAATCAGACCATGGATGATGAAACTTTGGCAGAGCCCATGGATATCGATAGCTGTGTTCCTGATGCGGACAATCGGAAAAGCTGGAGAAGCCCGCGTGAGCAGCTTCAGCATTGGCAGATGAATAGGAGGAAGCTGGCATTTTCAACAGTAGGGACGCCAGATTACATTGCTCCTGAAGTACTTTTGAAAAAAGGATATGGGATGGAATGTGATTGGTGGTCACTGGGAGCGATCATGTATGAAATGCTGGTCGGTTACCCTCCATTTTACTCTGATGATCCAATAACCACATGCAGAAAGATTGTTCATTGGAGAAACCATTTAAGATTTCCAGAAGATCCCCATGTAACACATGAGGCCAAGGATCTAATCTACAGATTGCTGTGTGATGTTGATCACAGGTTAGGTACCCGAGGGGCACAAGAAATCAAGGCTCATCCATGGTTCAAGGGTGTCGAGTGGGATAAACTATATGAAACGGAGGCAGCATTTAAACCACAAGTCAATGGGGAACTGGATACCCAAAACTTTATGAAGTTTGATGAGGTAGAACCACCAGATGCGGCAAGAGCTGGATCCGGATCTTCAAGAAAGATGCTTCTAACTCCCAAAGATCTAAACTTTGTTGGTTACACTTACAAGAACTTCGATGCTGTTAAAGAAGGTCTTCGTCAATCTTTGAGTGAGTCCATGCAAGATTATGCTTCCAAACGAACAGCCGATGAAACAGGCTTGCAAATGCTTGCGTCGTCTGGAGATCCTATGCTACCATAA |
| Protein sequence |
>MsaT017068.1 MDDLQEEGENGGGEEEVLGSSLTMEKVAAAKKFIENHYRAQMKNIQDRKERRWVLERKLATSDVPTEERLNLIKDLERKETEYMRLKRHKICVNDFDLLTIIGRGAFGEVRLCREKKSGNIYAMKKLKKSEMLRRGQVEHVRAERNLLAEVASHCIVKLYYSFQDTEYLYLIMEYLPGGDIMTLLMREDTLSENVARFYMAQSVLAIESIHKHNYIHRDIKPDNLLLDKNGHMKLSDFGLCKPIDCITLPTLHENQTMDDETLAEPMDIDSCVPDADNRKSWRSPREQLQHWQMNRRKLAFSTVGTPDYIAPEVLLKKGYGMECDWWSLGAIMYEMLVGYPPFYSDDPITTCRKIVHWRNHLRFPEDPHVTHEAKDLIYRLLCDVDHRLGTRGAQEIKAHPWFKGVEWDKLYETEAAFKPQVNGELDTQNFMKFDEVEPPDAARAGSGSSRKMLLTPKDLNFVGYTYKNFDAVKEGLRQSLSESMQDYASKRTADETGLQMLASSGDPMLP* |
