AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017078


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017078 MtrunA17_Chr3g0136081 81.579 532 45 7 1 492 1 519 0.0 843
MsaG017078 MtrunA17_Chr3g0105711 51.434 558 180 15 2 491 7 541 4.09e-159 462
MsaG017078 MtrunA17_Chr5g0406901 50.725 69 32 1 387 455 199 265 2.69e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017078 AT5G16100.2 32.721 272 128 11 252 476 177 440 3.29e-33 131

Find 104 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTTTCTGTGGGGCCATC+TGG 0.198703 3:+105501204 None:intergenic
GGCAATACATTGGAATTAAA+TGG 0.208227 3:+105502657 None:intergenic
TTCCAACTCCATGAAGATTT+TGG 0.209996 3:+105502778 None:intergenic
AATCTTATTCAAAATAATTC+AGG 0.275363 3:-105501684 MsaT017078.1:intron
ACCACAATGTTTAATCATTT+CGG 0.280881 3:+105500990 None:intergenic
CACCATCAGATACCTGTTAA+TGG 0.281140 3:-105502006 MsaT017078.1:CDS
TTTGAAAAGCTTGAGATTCA+TGG 0.294484 3:-105502057 MsaT017078.1:CDS
TTGGATTGATGATATTAATA+TGG 0.313839 3:-105501709 MsaT017078.1:CDS
CTTGGTGGTAGTAATAATAA+TGG 0.330532 3:-105502687 MsaT017078.1:CDS
ACCATCAGATACCTGTTAAT+GGG 0.352749 3:-105502005 MsaT017078.1:CDS
ACTGATGGGGCCCCATTAAC+AGG 0.356308 3:+105501994 None:intergenic
GGGGTCCATGCCGAGTTTGT+TGG 0.357115 3:+105502802 None:intergenic
TGATCTGCTGCTTCATCAAT+AGG 0.368145 3:+105501286 None:intergenic
TACTATGGACTTGGAATACT+TGG 0.370161 3:-105502705 MsaT017078.1:CDS
CAAGAGCAAATGGGTGATAA+TGG 0.372132 3:-105501500 MsaT017078.1:CDS
CCATCCCAAGAAAATTACTA+TGG 0.379676 3:-105502720 MsaT017078.1:CDS
AGATCTTCATCTTCCATGCT+TGG 0.390757 3:+105502945 None:intergenic
CTCTTTGCACTGGATTGGAT+TGG 0.394071 3:-105501728 MsaT017078.1:CDS
GGGTCCATGCCGAGTTTGTT+GGG 0.400094 3:+105502803 None:intergenic
AAAACGGGAATCTGAATTGC+AGG 0.401372 3:+105502282 None:intergenic
GTTTAATCATTTCGGTTTGA+AGG 0.403646 3:+105500998 None:intergenic
TCAAATTACTAGCAAATCAA+TGG 0.407732 3:-105501071 MsaT017078.1:CDS
TCTTATGTTTACAGATGAAT+TGG 0.410262 3:-105501528 MsaT017078.1:intron
TCGGTTTGGTTCAACAAAAC+GGG 0.413527 3:+105502267 None:intergenic
TTGATTAAGTGCCCGCAGTT+CGG 0.413996 3:+105502248 None:intergenic
TTCGGTTTGGTTCAACAAAA+CGG 0.419751 3:+105502266 None:intergenic
GATTCTAAATTGTAAGCAAA+TGG 0.423137 3:+105502606 None:intergenic
GTAATTTGATCTGCTTCAAA+AGG 0.424097 3:+105501085 None:intergenic
TGAGACTCTTTGCACTGGAT+TGG 0.424222 3:-105501733 MsaT017078.1:CDS
CAAGAAAATTACTATGGACT+TGG 0.426462 3:-105502714 MsaT017078.1:CDS
GATAAATCTGCTAGTGTTGT+TGG 0.428775 3:+105502867 None:intergenic
ACCTTTCTGTGGGGCCATCT+GGG 0.433450 3:+105501205 None:intergenic
GGACCCCACCAAAATCTTCA+TGG 0.435855 3:-105502786 MsaT017078.1:CDS
CCATGATATTCAACAAGGTC+AGG 0.445114 3:-105502523 MsaT017078.1:CDS
ATGAACTACCATCCACTTCA+AGG 0.454887 3:+105502337 None:intergenic
TCATATTGATCTTGAACCAT+TGG 0.458294 3:+105502396 None:intergenic
AAGAAAACTTGTCGTAAAGC+AGG 0.469657 3:-105501232 MsaT017078.1:CDS
CCTGACCTTGTTGAATATCA+TGG 0.476020 3:+105502523 None:intergenic
CACCAAAATCTTCATGGAGT+TGG 0.477847 3:-105502780 MsaT017078.1:CDS
TTATTCATATTGAAGAAACT+TGG 0.478548 3:+105502891 None:intergenic
TAAGTGCCCGCAGTTCGGTT+TGG 0.482116 3:+105502253 None:intergenic
ACTTGTTCTGAGAGGTTCCT+AGG 0.487950 3:+105501473 None:intergenic
AGGCAAGGGCAAGGACTGAA+GGG 0.491007 3:-105501032 MsaT017078.1:CDS
AGCAAATCAATGGGAAGGAC+AGG 0.496313 3:-105501061 MsaT017078.1:CDS
ATTGGTGGAGCAAGAGCAAA+TGG 0.500485 3:-105501510 MsaT017078.1:CDS
CAAATTACTAGCAAATCAAT+GGG 0.500638 3:-105501070 MsaT017078.1:CDS
TATCGCTGCTGCGGCTAAAG+TGG 0.502806 3:+105500925 None:intergenic
TATGTTTACAGATGAATTGG+TGG 0.515772 3:-105501525 MsaT017078.1:intron
AACTGCGGGCACTTAATCAA+TGG 0.515958 3:-105502245 MsaT017078.1:CDS
GAGGCAAGGGCAAGGACTGA+AGG 0.520542 3:-105501033 MsaT017078.1:CDS
TATTCATATTGAAGAAACTT+GGG 0.521262 3:+105502892 None:intergenic
TCAATCATTTGATAAACTGA+TGG 0.522363 3:+105501979 None:intergenic
GTCTCATAAAATTCAGACAT+TGG 0.526543 3:+105501750 None:intergenic
CAATCATTTGATAAACTGAT+GGG 0.528489 3:+105501980 None:intergenic
TTACCACTCTGATTCTGTTG+TGG 0.528506 3:+105502636 None:intergenic
GCTTGAGATTCATGGAAGGT+TGG 0.532570 3:-105502049 MsaT017078.1:CDS
ATTGAAGAAACTTGGGTCAA+CGG 0.535851 3:+105502899 None:intergenic
TGGAATAAATAGCCTTGAAG+TGG 0.539422 3:-105502349 MsaT017078.1:CDS
GTTGAACCAAACCGAACTGC+GGG 0.542214 3:-105502259 MsaT017078.1:CDS
ATAAATCTGCTAGTGTTGTT+GGG 0.542463 3:+105502868 None:intergenic
TATGGACTTGGAATACTTGG+TGG 0.542573 3:-105502702 MsaT017078.1:CDS
CTGCGGCTAAAGTGGCAAGA+AGG 0.552292 3:+105500933 None:intergenic
AGTTTGTTGGGACAGTGCAG+TGG 0.563322 3:+105502815 None:intergenic
ACCCAGATGGCCCCACAGAA+AGG 0.563782 3:-105501206 MsaT017078.1:intron
CCCCATTAACAGGTATCTGA+TGG 0.567348 3:+105502004 None:intergenic
GTAAAGCAGGGTTACCCAGA+TGG 0.568095 3:-105501219 MsaT017078.1:CDS
TGTGTGGAACGTCATTTAGC+AGG 0.571153 3:-105501792 MsaT017078.1:CDS
GTCCATGTTGTTGTACATCA+TGG 0.572620 3:+105502454 None:intergenic
AGAAAACTTGTCGTAAAGCA+GGG 0.586540 3:-105501231 MsaT017078.1:CDS
CGGCTAAAGTGGCAAGAAGG+CGG 0.587716 3:+105500936 None:intergenic
ATAAAATATGAATCCAAGCA+TGG 0.591295 3:-105502958 None:intergenic
ATTCTGTTGTGGCAATACAT+TGG 0.591773 3:+105502647 None:intergenic
CACCATGATGTACAACAACA+TGG 0.591982 3:-105502456 MsaT017078.1:CDS
AGGACAGGATACAGAGGCAA+GGG 0.593438 3:-105501046 MsaT017078.1:CDS
CTGTCCCAACAAACTCGGCA+TGG 0.594201 3:-105502807 MsaT017078.1:CDS
GTTTGTTGGGACAGTGCAGT+GGG 0.598692 3:+105502816 None:intergenic
GATATTCAACAAGGTCAGGA+TGG 0.598749 3:-105502519 MsaT017078.1:CDS
ATAAATAGCCTTGAAGTGGA+TGG 0.603975 3:-105502345 MsaT017078.1:CDS
CTGCACTGTCCCAACAAACT+CGG 0.604462 3:-105502812 MsaT017078.1:CDS
GAAATGATTAAACATTGTGG+TGG 0.607415 3:-105500988 MsaT017078.1:CDS
TGAATATCATGGTAAATGAA+CGG 0.610177 3:+105502534 None:intergenic
ACCGAAATGATTAAACATTG+TGG 0.612061 3:-105500991 MsaT017078.1:CDS
CCATCAGATACCTGTTAATG+GGG 0.617286 3:-105502004 MsaT017078.1:CDS
AAAAGCTTGAGATTCATGGA+AGG 0.617730 3:-105502053 MsaT017078.1:CDS
GGCAAGGGCAAGGACTGAAG+GGG 0.618518 3:-105501031 MsaT017078.1:CDS
TTACTAGCAAATCAATGGGA+AGG 0.619972 3:-105501066 MsaT017078.1:CDS
TGTTGAACCAAACCGAACTG+CGG 0.627319 3:-105502260 MsaT017078.1:CDS
CAACAACATGGACATGGACA+TGG 0.630152 3:-105502444 MsaT017078.1:CDS
CATCATATTCAACAACTGCA+TGG 0.630346 3:-105502570 MsaT017078.1:CDS
AGAGAAATCATACACACTGA+TGG 0.630751 3:-105502177 MsaT017078.1:intron
AAATACGGGGTTACTGTGGG+TGG 0.632071 3:+105502222 None:intergenic
GCGATGTCATACAACGCGGT+TGG 0.634750 3:+105500958 None:intergenic
AGGATACAGAGGCAAGGGCA+AGG 0.638191 3:-105501041 MsaT017078.1:CDS
AAGGACAGGATACAGAGGCA+AGG 0.639471 3:-105501047 MsaT017078.1:CDS
ATTTACCATGATATTCAACA+AGG 0.642604 3:-105502528 MsaT017078.1:CDS
ATATTATTACTTGTTCTGAG+AGG 0.643167 3:+105501465 None:intergenic
GATGTACAACAACATGGACA+TGG 0.659086 3:-105502450 MsaT017078.1:CDS
AATCATTTGATAAACTGATG+GGG 0.687280 3:+105501981 None:intergenic
TTGGTGGAGCAAGAGCAAAT+GGG 0.694143 3:-105501509 MsaT017078.1:CDS
ATGGGAAGGACAGGATACAG+AGG 0.708986 3:-105501052 MsaT017078.1:CDS
TGGGTGATAATGGAACGCCT+AGG 0.709329 3:-105501490 MsaT017078.1:CDS
CATATTCAACAACTGCATGG+TGG 0.712817 3:-105502567 MsaT017078.1:CDS
TTGCCACAACAGAATCAGAG+TGG 0.727563 3:-105502639 MsaT017078.1:CDS
GGCGGCGATGTCATACAACG+CGG 0.812023 3:+105500954 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 105500939 105502973 105500939 ID=MsaG017078
Chr3 mRNA 105500939 105502973 105500939 ID=MsaT017078.1;Parent=MsaG017078
Chr3 exon 105500939 105501115 105500939 ID=MsaT017078.1.exon6;Parent=MsaT017078.1
Chr3 CDS 105500939 105501115 105500939 ID=cds.MsaT017078.1;Parent=MsaT017078.1
Chr3 exon 105501207 105501362 105501207 ID=MsaT017078.1.exon5;Parent=MsaT017078.1
Chr3 CDS 105501207 105501362 105501207 ID=cds.MsaT017078.1;Parent=MsaT017078.1
Chr3 exon 105501475 105501536 105501475 ID=MsaT017078.1.exon4;Parent=MsaT017078.1
Chr3 CDS 105501475 105501536 105501475 ID=cds.MsaT017078.1;Parent=MsaT017078.1
Chr3 exon 105501685 105501863 105501685 ID=MsaT017078.1.exon3;Parent=MsaT017078.1
Chr3 CDS 105501685 105501863 105501685 ID=cds.MsaT017078.1;Parent=MsaT017078.1
Chr3 exon 105501979 105502087 105501979 ID=MsaT017078.1.exon2;Parent=MsaT017078.1
Chr3 CDS 105501979 105502087 105501979 ID=cds.MsaT017078.1;Parent=MsaT017078.1
Chr3 exon 105502178 105502973 105502178 ID=MsaT017078.1.exon1;Parent=MsaT017078.1
Chr3 CDS 105502178 105502973 105502178 ID=cds.MsaT017078.1;Parent=MsaT017078.1
Gene Sequence

>MsaT017078.1

ATGAATCCAAGCATGGAAGATGAAGATCTCATGTTTGACTGCTTCGAAAATTCCGTTGACCCAAGTTTCTTCAATATGAATAACCCAACAACACTAGCAGATTTATCCATTGATAAAAATAACAACAATGTGGGTCCCACTGCACTGTCCCAACAAACTCGGCATGGACCCCACCAAAATCTTCATGGAGTTGGAATAGTTAGTAATTTTAATGATCTTTTTTCCACTCCACCATCCCAAGAAAATTACTATGGACTTGGAATACTTGGTGGTAGTAATAATAATGGAAATGATCCATTTAATTCCAATGTATTGCCACAACAGAATCAGAGTGGTAATAATGATCCATTTGCTTACAATTTAGAATCACCAGAAAATGATCATCATATTCAACAACTGCATGGTGGTAGTAATGATCCGTTCATTTACCATGATATTCAACAAGGTCAGGATGGTAATATTGATTCATTTTCGGATGTTATGTTACAACAAAATCACCATGATGTACAACAACATGGACATGGACATGGTAGTACAAATGAAACATTTTCTGTTCCAATGGTTCAAGATCAATATGATCAAAACAATAATAATTTTTCATTTGGAATAAATAGCCTTGAAGTGGATGGTAGTTCATCACGAATGTATGAGAATCAACAACAAAGTATACCTGCAATTCAGATTCCCGTTTTGTTGAACCAAACCGAACTGCGGGCACTTAATCAATGGCCACCCACAGTAACCCCGTATTTTTGCAGTTGTTGTCAAGTTCTCAGAGAAATCATACACACTGATGGTATGCAATTTGAAAAGCTTGAGATTCATGGAAGGTTGGCGTTGATTACACATGCAATTCACCATCAGATACCTGTTAATGGGGCCCCATCAGTTTATCAAATGATTGATTTTTGTGGGAAAAGCCTTGGTGAAATAAAGAATTTTTTGGCACAGTACTGTGTGGAACGTCATTTAGCAGGATACATTCTTATGCAAGATCCAATGTCTGAATTTTATGAGACTCTTTGCACTGGATTGGATTGGATTGATGATATTAATATGGAGAATCTTATTCAAAATAATTCAGATGAATTGGTGGAGCAAGAGCAAATGGGTGATAATGGAACGCCTAGGAACCTCTCAGAACAAAGGAAAAAATCTGCAAAACTGAAATTGAGTGATTTATGTGATTACTTTCATCTACCTATTGATGAAGCAGCAGATCATGAGAAAGTGAAGTTATGTCCAACAGTTCTCAAGAAAACTTGTCGTAAAGCAGGGTTACCCAGATGGCCCCACAGAAAGGTGAAAAGCCTTTTGAAGCAGATCAAATTACTAGCAAATCAATGGGAAGGACAGGATACAGAGGCAAGGGCAAGGACTGAAGGGGAAATTAAAAGCCTTCAAACCGAAATGATTAAACATTGTGGTGGACATATACCAACCGCGTTGTATGACATCGCCGCCTTCTTGCCACTTTAG

Protein sequence

>MsaT017078.1

MNPSMEDEDLMFDCFENSVDPSFFNMNNPTTLADLSIDKNNNNVGPTALSQQTRHGPHQNLHGVGIVSNFNDLFSTPPSQENYYGLGILGGSNNNGNDPFNSNVLPQQNQSGNNDPFAYNLESPENDHHIQQLHGGSNDPFIYHDIQQGQDGNIDSFSDVMLQQNHHDVQQHGHGHGSTNETFSVPMVQDQYDQNNNNFSFGINSLEVDGSSSRMYENQQQSIPAIQIPVLLNQTELRALNQWPPTVTPYFCSCCQVLREIIHTDGMQFEKLEIHGRLALITHAIHHQIPVNGAPSVYQMIDFCGKSLGEIKNFLAQYCVERHLAGYILMQDPMSEFYETLCTGLDWIDDINMENLIQNNSDELVEQEQMGDNGTPRNLSEQRKKSAKLKLSDLCDYFHLPIDEAADHEKVKLCPTVLKKTCRKAGLPRWPHRKVKSLLKQIKLLANQWEGQDTEARARTEGEIKSLQTEMIKHCGGHIPTALYDIAAFLPL*