AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017127

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017127 MtrunA17_Chr1g0205561 89.683 126 13 0 1 126 364 489 6.46e-78 238
MsaG017127 MtrunA17_Chr5g0394381 47.581 124 46 2 1 124 657 761 2.44e-31 117
MsaG017127 MtrunA17_Chr2g0311511 43.802 121 63 3 2 122 610 725 8.41e-30 112
MsaG017127 MtrunA17_Chr5g0399561 42.149 121 66 2 2 122 678 794 8.36e-29 109
MsaG017127 MtrunA17_Chr2g0311541 44.628 121 62 2 2 122 664 779 1.17e-28 109
MsaG017127 MtrunA17_Chr2g0311521 44.628 121 62 2 2 122 907 1022 1.80e-28 108
MsaG017127 MtrunA17_Chr2g0311551 43.802 121 63 2 2 122 901 1016 3.32e-28 107
MsaG017127 MtrunA17_Chr5g0399591 41.322 121 67 2 2 122 675 791 1.36e-27 106
MsaG017127 MtrunA17_Chr8g0338501 42.975 121 65 1 2 122 719 835 8.46e-26 100
MsaG017127 MtrunA17_Chr8g0338471 42.149 121 66 1 2 122 688 804 1.38e-25 100
MsaG017127 MtrunA17_Chr8g0338531 38.333 120 70 1 2 121 623 738 2.40e-20 85.5
MsaG017127 MtrunA17_Chr4g0063701 33.058 121 73 3 2 122 693 805 1.04e-19 83.6
MsaG017127 MtrunA17_Chr4g0063691 39.167 120 67 3 4 122 706 820 4.10e-19 82.0
MsaG017127 MtrunA17_Chr3g0137741 34.711 121 73 1 2 122 658 772 1.14e-17 77.8
MsaG017127 MtrunA17_Chr6g0467301 37.903 124 66 4 2 122 661 776 1.28e-17 77.8
MsaG017127 MtrunA17_Chr2g0314611 35.246 122 72 2 2 122 651 766 1.55e-17 77.4
MsaG017127 MtrunA17_Chr6g0467281 36.719 128 68 4 2 124 656 775 1.56e-17 77.4
MsaG017127 MtrunA17_Chr8g0366981 34.400 125 71 4 1 123 466 581 1.97e-17 77.0
MsaG017127 MtrunA17_Chr8g0366971 35.200 125 70 4 1 123 240 355 6.16e-17 75.5
MsaG017127 MtrunA17_Chr7g0274661 33.333 111 68 1 1 111 539 643 9.23e-17 75.1
MsaG017127 MtrunA17_Chr2g0318171 35.772 123 70 3 2 122 647 762 1.45e-16 74.7
MsaG017127 MtrunA17_Chr8g0366931 33.600 125 72 4 1 123 190 305 1.90e-16 73.9
MsaG017127 MtrunA17_Chr5g0433941 35.246 122 78 1 1 122 533 653 2.80e-16 73.9
MsaG017127 MtrunA17_Chr8g0348661 28.571 126 86 1 1 122 648 773 9.76e-16 72.4
MsaG017127 MtrunA17_Chr1g0158751 32.800 125 74 4 1 122 261 378 1.17e-15 72.0
MsaG017127 MtrunA17_Chr4g0063251 37.795 127 69 4 2 122 274 396 1.41e-15 71.6
MsaG017127 MtrunA17_Chr1g0161631 32.800 125 76 3 2 121 664 785 1.72e-15 71.6
MsaG017127 MtrunA17_Chr7g0239371 32.500 120 71 3 1 118 504 615 2.31e-15 71.2
MsaG017127 MtrunA17_Chr1g0202331 29.032 124 80 2 2 123 700 817 4.66e-15 70.5
MsaG017127 MtrunA17_Chr6g0484051 35.200 125 70 5 1 122 516 632 5.87e-15 70.1
MsaG017127 MtrunA17_Chr2g0278851 33.043 115 72 1 1 115 438 547 7.48e-15 69.7
MsaG017127 MtrunA17_Chr1g0149381 31.405 121 77 1 2 122 667 781 7.53e-15 69.7
MsaG017127 MtrunA17_Chr2g0278871 30.435 115 76 1 1 115 461 571 9.75e-15 69.3
MsaG017127 MtrunA17_Chr2g0333091 33.058 121 75 1 2 122 658 772 9.86e-15 69.3
MsaG017127 MtrunA17_Chr1g0158571 33.600 125 73 5 1 122 378 495 1.29e-14 68.9
MsaG017127 MtrunA17_Chr3g0102451 36.036 111 64 1 1 111 492 595 1.42e-14 68.9
MsaG017127 MtrunA17_Chr2g0323301 34.074 135 58 5 2 123 661 777 1.43e-14 68.9
MsaG017127 MtrunA17_Chr1g0200911 31.304 115 75 1 1 115 471 581 1.50e-14 68.9
MsaG017127 MtrunA17_Chr2g0306811 33.333 123 80 1 2 122 276 398 1.72e-14 68.6
MsaG017127 MtrunA17_Chr2g0278831 30.435 115 76 1 1 115 460 570 1.78e-14 68.6
MsaG017127 MtrunA17_Chr2g0297921 34.959 123 72 3 2 123 310 425 2.68e-14 68.2
MsaG017127 MtrunA17_Chr1g0159611 31.405 121 76 1 2 122 409 522 2.69e-14 68.2
MsaG017127 MtrunA17_Chr8g0380681 34.146 123 73 3 2 121 1157 1274 2.85e-14 68.2
MsaG017127 MtrunA17_Chr8g0380681 32.800 125 75 2 2 122 487 606 3.87e-12 62.0
MsaG017127 MtrunA17_Chr2g0297951 32.576 132 63 3 2 123 300 415 3.12e-14 67.8
MsaG017127 MtrunA17_Chr7g0239381 30.833 120 73 3 1 118 508 619 3.24e-14 67.8
MsaG017127 MtrunA17_Chr5g0413291 29.565 115 76 1 1 115 469 578 3.24e-14 67.8
MsaG017127 MtrunA17_Chr7g0274651 33.333 123 74 4 1 123 535 649 3.41e-14 67.8
MsaG017127 MtrunA17_Chr3g0106131 34.188 117 67 3 2 111 675 788 3.82e-14 67.8
MsaG017127 MtrunA17_Chr2g0323341 31.579 133 64 4 2 123 574 690 3.84e-14 67.8
MsaG017127 MtrunA17_Chr4g0053161 31.667 120 71 3 2 115 698 812 4.52e-14 67.4
MsaG017127 MtrunA17_Chr2g0314841 33.613 119 69 4 2 118 828 938 4.78e-14 67.4
MsaG017127 MtrunA17_Chr2g0314821 33.613 119 69 4 2 118 831 941 4.78e-14 67.4
MsaG017127 MtrunA17_Chr8g0367071 33.333 123 74 2 1 123 520 634 4.97e-14 67.4
MsaG017127 MtrunA17_Chr1g0158631 31.200 125 76 4 1 122 549 666 5.14e-14 67.4
MsaG017127 MtrunA17_Chr3g0123891 30.435 115 76 1 1 115 453 563 6.13e-14 67.0
MsaG017127 MtrunA17_Chr1g0158661 32.000 125 75 4 1 122 535 652 8.33e-14 66.6
MsaG017127 MtrunA17_Chr7g0220741 31.304 115 74 1 1 115 469 578 8.97e-14 66.6
MsaG017127 MtrunA17_Chr4g0023071 31.967 122 78 2 2 123 659 775 1.34e-13 66.2
MsaG017127 MtrunA17_Chr8g0366991 30.645 124 75 4 2 123 636 750 1.47e-13 66.2
MsaG017127 MtrunA17_Chr4g0049551 33.607 122 73 2 2 123 526 639 1.59e-13 65.9
MsaG017127 MtrunA17_Chr1g0158041 32.787 122 66 5 1 117 556 666 1.80e-13 65.9
MsaG017127 MtrunA17_Chr0c04g0492031 31.034 116 74 2 2 111 684 799 1.87e-13 65.9
MsaG017127 MtrunA17_Chr3g0134291 31.034 116 74 2 2 111 684 799 1.87e-13 65.9
MsaG017127 MtrunA17_Chr3g0106141 33.333 117 68 3 2 111 678 791 2.14e-13 65.5
MsaG017127 MtrunA17_Chr0c04g0492001 31.034 116 74 2 2 111 684 799 2.15e-13 65.5
MsaG017127 MtrunA17_Chr3g0106181 34.188 117 67 3 2 111 222 335 2.19e-13 65.1
MsaG017127 MtrunA17_Chr8g0358781 31.933 119 74 2 1 119 508 619 2.93e-13 65.1
MsaG017127 MtrunA17_Chr8g0385051 31.579 114 73 2 1 114 440 548 3.56e-13 65.1
MsaG017127 MtrunA17_Chr2g0278861 30.435 115 76 1 1 115 456 566 5.58e-13 64.3
MsaG017127 MtrunA17_Chr2g0277731 33.594 128 73 3 2 122 483 605 8.20e-13 63.9
MsaG017127 MtrunA17_Chr4g0022301 29.412 136 79 7 1 128 8 134 8.36e-13 61.2
MsaG017127 MtrunA17_Chr5g0446651 33.871 124 70 4 1 121 525 639 1.01e-12 63.5
MsaG017127 MtrunA17_Chr3g0130431 29.661 118 77 2 2 119 512 623 1.61e-12 63.2
MsaG017127 MtrunA17_Chr7g0253471 31.496 127 72 6 1 122 50 166 1.99e-12 61.2
MsaG017127 MtrunA17_Chr1g0157881 31.200 125 76 6 1 122 631 748 2.02e-12 62.8
MsaG017127 MtrunA17_Chr7g0251051 30.709 127 74 6 1 122 481 598 2.22e-12 62.8
MsaG017127 MtrunA17_Chr7g0250741 31.538 130 69 7 1 122 702 819 2.24e-12 62.8
MsaG017127 MtrunA17_Chr7g0250931 31.746 126 74 6 1 122 716 833 2.31e-12 62.8
MsaG017127 MtrunA17_Chr5g0413171 29.104 134 80 5 1 125 253 380 3.07e-12 62.0
MsaG017127 MtrunA17_Chr7g0237341 32.456 114 70 3 2 111 675 785 3.62e-12 62.0
MsaG017127 MtrunA17_Chr7g0237331 28.448 116 72 2 2 111 676 786 3.69e-12 62.0
MsaG017127 MtrunA17_Chr8g0358821 28.814 118 77 2 1 118 853 963 3.74e-12 62.0
MsaG017127 MtrunA17_Chr0c03g0490031 28.696 115 77 1 1 115 464 573 4.48e-12 61.6
MsaG017127 MtrunA17_Chr4g0049601 32.000 125 72 4 1 123 523 636 4.59e-12 61.6
MsaG017127 MtrunA17_Chr1g0158761 34.109 129 66 7 1 122 500 616 5.37e-12 61.6
MsaG017127 MtrunA17_Chr1g0158191 30.469 128 72 7 1 122 556 672 5.53e-12 61.6
MsaG017127 MtrunA17_Chr6g0456231 29.565 115 76 1 1 115 467 576 6.00e-12 61.6
MsaG017127 MtrunA17_Chr8g0382101 28.696 115 78 1 1 115 450 560 6.21e-12 61.2
MsaG017127 MtrunA17_Chr4g0049581 30.400 125 76 4 1 123 515 630 8.63e-12 60.8
MsaG017127 MtrunA17_Chr3g0078761 31.148 122 76 2 1 121 512 626 8.65e-12 60.8
MsaG017127 MtrunA17_Chr1g0157931 31.200 125 76 6 1 122 438 555 1.07e-11 60.8
MsaG017127 MtrunA17_Chr7g0251071 31.496 127 74 6 1 122 521 639 1.08e-11 60.8
MsaG017127 MtrunA17_Chr1g0158391 30.769 117 72 3 1 115 423 532 1.21e-11 60.5
MsaG017127 MtrunA17_Chr2g0284101 26.613 124 82 2 1 122 465 581 1.27e-11 60.5
MsaG017127 MtrunA17_Chr7g0251101 30.400 125 77 4 1 122 527 644 1.64e-11 60.1
MsaG017127 MtrunA17_Chr1g0157801 34.127 126 68 6 1 122 464 578 1.70e-11 60.1
MsaG017127 MtrunA17_Chr5g0409041 27.559 127 79 3 1 124 469 585 1.78e-11 60.1
MsaG017127 MtrunA17_Chr1g0157781 34.127 126 68 6 1 122 469 583 2.27e-11 59.7
MsaG017127 MtrunA17_Chr7g0251031 32.283 127 73 6 1 122 94 212 2.46e-11 58.9
MsaG017127 MtrunA17_Chr2g0315081 31.304 115 69 3 2 114 821 927 2.82e-11 59.7
MsaG017127 MtrunA17_Chr1g0158151 29.688 128 73 6 1 122 551 667 2.95e-11 59.3
MsaG017127 MtrunA17_Chr2g0314961 30.400 125 74 4 2 124 303 416 3.12e-11 59.3
MsaG017127 MtrunA17_Chr1g0157951 29.688 128 73 7 1 122 346 462 3.62e-11 59.3
MsaG017127 MtrunA17_Chr5g0414561 29.167 120 75 3 1 118 511 622 4.86e-11 58.9
MsaG017127 MtrunA17_Chr1g0158481 29.600 125 77 6 1 122 91 207 5.06e-11 58.2
MsaG017127 MtrunA17_Chr2g0315151 29.268 123 77 3 1 121 832 946 6.17e-11 58.5
MsaG017127 MtrunA17_Chr8g0389251 30.769 117 71 4 1 115 569 677 6.87e-11 58.5
MsaG017127 MtrunA17_Chr1g0158771 29.600 125 77 5 1 122 455 571 7.82e-11 58.2
MsaG017127 MtrunA17_Chr2g0314901 29.600 125 75 4 2 124 836 949 8.83e-11 58.2
MsaG017127 MtrunA17_Chr4g0033571 31.538 130 82 2 1 129 687 810 9.46e-11 58.2
MsaG017127 MtrunA17_Chr4g0022331 28.676 136 80 7 1 128 700 826 9.48e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017127 AT4G32300.1 35.484 124 69 3 2 122 659 774 3.38e-17 77.0
MsaG017127 AT5G03140.1 34.959 123 79 1 1 123 537 658 9.31e-17 75.5
MsaG017127 AT1G34300.1 35.484 124 68 3 2 121 650 765 1.22e-16 75.5
MsaG017127 AT1G60800.2 29.565 115 76 1 1 115 401 510 2.41e-15 71.6
MsaG017127 AT1G60800.1 29.565 115 76 1 1 115 468 577 2.55e-15 71.6
MsaG017127 AT1G71830.1 31.304 115 75 1 1 115 469 579 3.68e-15 70.9
MsaG017127 AT1G34210.1 31.304 115 75 1 1 115 472 582 3.86e-15 70.9
MsaG017127 AT1G34210.2 31.304 115 75 1 1 115 472 582 3.86e-15 70.9
MsaG017127 AT4G33430.2 32.174 115 74 1 1 115 503 613 6.69e-15 70.5
MsaG017127 AT4G33430.1 32.174 115 74 1 1 115 456 566 6.69e-15 70.1
MsaG017127 AT3G25560.1 33.043 115 72 1 1 115 475 584 7.05e-15 70.1
MsaG017127 AT3G25560.2 33.043 115 72 1 1 115 476 585 7.06e-15 70.1
MsaG017127 AT2G19130.1 32.308 130 68 4 2 123 660 777 7.06e-15 70.1
MsaG017127 AT3G25560.3 33.043 115 72 1 1 115 475 584 7.07e-15 70.1
MsaG017127 AT3G53380.1 34.426 122 80 0 1 122 540 661 1.55e-14 69.3
MsaG017127 AT1G29730.1 30.508 118 77 1 1 118 805 917 3.37e-14 68.2
MsaG017127 AT1G29730.2 30.508 118 77 1 1 118 837 949 3.38e-14 68.2
MsaG017127 AT1G70250.1 28.000 125 84 3 1 122 624 745 4.06e-14 68.2
MsaG017127 AT5G24080.3 29.268 123 80 2 2 123 570 686 4.36e-14 68.2
MsaG017127 AT5G24080.1 29.268 123 80 2 2 123 570 686 4.36e-14 68.2
MsaG017127 AT5G24080.2 29.268 123 80 2 2 123 697 813 4.42e-14 68.2
MsaG017127 AT5G65240.1 32.479 117 71 3 1 115 451 561 8.54e-14 67.0
MsaG017127 AT5G65240.2 32.479 117 71 3 1 115 451 561 9.29e-14 67.0
MsaG017127 AT2G13800.4 29.565 115 77 1 1 115 325 435 1.22e-13 66.6
MsaG017127 AT2G13800.2 29.565 115 77 1 1 115 325 435 1.22e-13 66.6
MsaG017127 AT4G00340.2 35.246 122 63 5 2 114 626 740 1.58e-13 66.2
MsaG017127 AT3G59740.1 30.702 114 73 1 1 114 502 609 1.70e-13 66.2
MsaG017127 AT4G00340.1 35.246 122 63 5 2 114 647 761 1.72e-13 66.2
MsaG017127 AT4G02420.1 30.702 114 74 1 2 115 516 624 1.79e-13 66.2
MsaG017127 AT1G29740.2 31.532 111 71 1 1 111 841 946 2.39e-13 65.9
MsaG017127 AT1G29740.1 31.532 111 71 1 1 111 841 946 2.40e-13 65.9
MsaG017127 AT2G13800.3 29.565 115 77 1 1 115 442 552 2.43e-13 65.9
MsaG017127 AT2G13800.1 29.565 115 77 1 1 115 442 552 2.43e-13 65.9
MsaG017127 AT2G13790.1 29.565 115 77 1 1 115 461 571 2.44e-13 65.9
MsaG017127 AT1G11050.1 35.115 131 71 4 2 125 467 590 2.79e-13 65.5
MsaG017127 AT1G31420.2 27.642 123 82 2 2 124 470 585 5.05e-13 65.1
MsaG017127 AT1G31420.1 27.642 123 82 2 2 124 471 586 5.36e-13 64.7
MsaG017127 AT1G31420.3 27.642 123 82 2 2 124 471 586 5.36e-13 64.7
MsaG017127 AT5G55830.1 31.967 122 78 1 1 122 533 649 5.67e-13 64.7
MsaG017127 AT1G53430.2 31.200 125 75 4 1 123 794 909 6.54e-13 64.7
MsaG017127 AT1G53430.1 31.200 125 75 4 1 123 835 950 6.56e-13 64.7
MsaG017127 AT1G66980.4 32.308 130 72 7 1 122 952 1073 6.98e-13 64.7
MsaG017127 AT1G66980.1 32.308 130 72 7 1 122 969 1090 6.99e-13 64.7
MsaG017127 AT1G66980.3 32.308 130 72 7 1 122 996 1117 7.00e-13 64.7
MsaG017127 AT1G66980.2 32.308 130 72 7 1 122 998 1119 7.00e-13 64.7
MsaG017127 AT4G28350.1 31.579 114 66 3 2 113 517 620 1.12e-12 63.9
MsaG017127 AT3G53810.1 32.540 126 73 4 1 123 512 628 1.28e-12 63.9
MsaG017127 AT1G53440.1 30.400 125 76 4 1 123 833 948 1.58e-12 63.5
MsaG017127 AT5G65600.1 34.921 126 74 5 2 123 516 637 2.03e-12 63.2
MsaG017127 AT3G55550.1 31.250 128 75 4 2 124 513 632 2.70e-12 62.8
MsaG017127 AT5G10290.1 29.915 117 74 3 1 115 457 567 2.82e-12 62.8
MsaG017127 AT5G10290.2 29.915 117 74 3 1 115 457 567 2.82e-12 62.8
MsaG017127 AT4G02410.1 31.579 114 73 1 2 115 521 629 2.91e-12 62.8
MsaG017127 AT2G32800.1 33.071 127 73 4 1 123 309 427 2.95e-12 62.8
MsaG017127 AT3G14840.2 29.457 129 81 3 2 128 839 959 3.57e-12 62.4
MsaG017127 AT2G23950.1 29.565 115 76 1 1 115 462 571 4.21e-12 62.4
MsaG017127 AT1G29720.1 30.579 121 74 4 1 119 837 949 5.10e-12 62.0
MsaG017127 AT1G67520.4 31.298 131 79 5 2 126 620 745 5.82e-12 62.0
MsaG017127 AT1G67520.6 31.298 131 79 5 2 126 670 795 5.85e-12 62.0
MsaG017127 AT1G67520.3 31.298 131 79 5 2 126 663 788 5.96e-12 62.0
MsaG017127 AT5G38260.1 30.534 131 72 7 1 122 485 605 7.46e-12 61.6
MsaG017127 AT1G67000.1 27.273 132 84 5 1 122 721 850 8.27e-12 61.6
MsaG017127 AT1G67520.8 31.061 132 80 5 1 126 454 580 9.27e-12 61.2
MsaG017127 AT1G67520.7 31.061 132 80 5 1 126 454 580 9.27e-12 61.2
MsaG017127 AT1G67520.5 31.061 132 80 5 1 126 425 551 9.76e-12 61.2
MsaG017127 AT4G18250.1 34.375 128 74 6 1 122 521 644 9.87e-12 61.2
MsaG017127 AT1G07650.4 29.310 116 72 3 1 114 632 739 1.05e-11 61.2
MsaG017127 AT1G07650.3 29.310 116 72 3 1 114 819 926 1.07e-11 61.2
MsaG017127 AT1G07650.1 29.310 116 72 3 1 114 845 952 1.07e-11 61.2
MsaG017127 AT1G07650.2 29.310 116 72 3 1 114 851 958 1.08e-11 61.2
MsaG017127 AT2G16750.2 27.966 118 79 2 2 119 441 552 1.51e-11 60.8
MsaG017127 AT2G16750.1 27.966 118 79 2 2 119 441 552 1.51e-11 60.8
MsaG017127 AT4G11480.4 28.571 126 82 4 2 124 488 608 1.70e-11 60.5
MsaG017127 AT4G11480.3 28.571 126 82 4 2 124 491 611 1.70e-11 60.5
MsaG017127 AT4G11480.1 28.571 126 82 4 2 124 496 616 1.70e-11 60.5
MsaG017127 AT5G38280.1 29.839 124 80 5 2 122 497 616 3.23e-11 59.7
MsaG017127 AT5G38280.2 29.839 124 80 5 2 122 497 616 3.24e-11 59.7
MsaG017127 AT4G35030.9 32.727 110 68 2 2 111 132 235 4.65e-11 58.9
MsaG017127 AT5G63940.1 29.661 118 77 2 2 119 529 640 5.08e-11 59.3
MsaG017127 AT5G39020.1 28.800 125 80 4 1 122 660 778 8.28e-11 58.5
MsaG017127 AT3G13690.2 31.034 116 70 3 2 115 577 684 9.06e-11 58.5
MsaG017127 AT3G13690.1 31.034 116 70 3 2 115 577 684 9.06e-11 58.5
MsaG017127 AT3G13690.3 31.034 116 70 3 2 115 577 684 9.06e-11 58.5

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATAGATGGCCTAATAAT+TGG 0.179313 3:-106025922 None:intergenic
TTATAGATTGGGTTCTATAT+TGG 0.218702 3:+106025788 MsaT017127.1:CDS
TATAGATTGGGTTCTATATT+GGG 0.279264 3:+106025789 MsaT017127.1:CDS
GATGGCCTAATAATTGGATT+AGG 0.304889 3:-106025916 None:intergenic
TGTGCCCTAATCCAATTATT+AGG 0.308361 3:+106025911 MsaT017127.1:CDS
TGCAACACCTTTGCAATAGA+TGG 0.340385 3:-106025934 None:intergenic
ATGACATGATACTTATAGAT+TGG 0.369608 3:+106025776 MsaT017127.1:CDS
AGTGTTCCACCATTGTTTGA+TGG 0.394571 3:+106025982 MsaT017127.1:CDS
AAGTTGTTAATGATTTCATT+AGG 0.396201 3:+106025854 MsaT017127.1:CDS
TAGAGGGACAATGGGATGTT+TGG 0.414762 3:+106025618 None:intergenic
ATGGCCTAATAATTGGATTA+GGG 0.442397 3:-106025915 None:intergenic
TAGGTTTGAGAGAATGACTA+TGG 0.451333 3:+106025873 MsaT017127.1:CDS
TGACATGATACTTATAGATT+GGG 0.452945 3:+106025777 MsaT017127.1:CDS
GGTTCTATATTGGGCAAAAG+AGG 0.465154 3:+106025798 MsaT017127.1:CDS
GAATTGCATCAAGTTGAAGA+TGG 0.493224 3:+106025739 MsaT017127.1:CDS
TGCACCTATCACTGCAAAAG+TGG 0.498118 3:+106025660 MsaT017127.1:CDS
AAAGTGGATATCTACAGCTT+TGG 0.498218 3:+106025676 MsaT017127.1:CDS
TTTGAGAGAATGACTATGGT+TGG 0.507037 3:+106025877 MsaT017127.1:CDS
GTTCTATATTGGGCAAAAGA+GGG 0.512150 3:+106025799 MsaT017127.1:CDS
CATTAACAACTTCAACATCA+TGG 0.537170 3:-106025843 None:intergenic
TGCAAAGGTGTTGCAGATGT+CGG 0.550893 3:+106025942 MsaT017127.1:CDS
CAAGTTGAAGATGGAACAGA+AGG 0.575586 3:+106025748 MsaT017127.1:CDS
TATTAGGCCATCTATTGCAA+AGG 0.594426 3:+106025927 MsaT017127.1:CDS
TTCTATATTGGGCAAAAGAG+GGG 0.600711 3:+106025800 MsaT017127.1:CDS
TGGGATGTTTGGTACCAGAA+TGG 0.601392 3:+106025629 MsaT017127.1:CDS
AAGGTGTTGCAGATGTCGGA+AGG 0.614948 3:+106025946 MsaT017127.1:CDS
GTTGAAGATGGAACAGAAGG+TGG 0.634859 3:+106025751 MsaT017127.1:CDS
GAATGACTATGGTTGGACTG+TGG 0.741878 3:+106025884 MsaT017127.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 106025628 106026017 106025628 ID=MsaG017127
Chr3 mRNA 106025628 106026017 106025628 ID=MsaT017127.1;Parent=MsaG017127
Chr3 exon 106025628 106026017 106025628 ID=MsaT017127.1.exon1;Parent=MsaT017127.1
Chr3 CDS 106025628 106026017 106025628 ID=cds.MsaT017127.1;Parent=MsaT017127.1
Gene Sequence

>MsaT017127.1

ATGGGATGTTTGGTACCAGAATGGCTCAAAAATGCACCTATCACTGCAAAAGTGGATATCTACAGCTTTGGTGTCATGTTGCTTGAAATCTTATTTTGTAGAAGGCACATTGAATTGCATCAAGTTGAAGATGGAACAGAAGGTGGTGATGACATGATACTTATAGATTGGGTTCTATATTGGGCAAAAGAGGGGAAACTAAGAGACATTCTTAGCCATGATGTTGAAGTTGTTAATGATTTCATTAGGTTTGAGAGAATGACTATGGTTGGACTGTGGTGCTTGTGCCCTAATCCAATTATTAGGCCATCTATTGCAAAGGTGTTGCAGATGTCGGAAGGAGATTTTGAAGTTAGTGTTCCACCATTGTTTGATGGAAAAATTTTGTAA
Protein sequence

>MsaT017127.1

MGCLVPEWLKNAPITAKVDIYSFGVMLLEILFCRRHIELHQVEDGTEGGDDMILIDWVLYWAKEGKLRDILSHDVEVVNDFIRFERMTMVGLWCLCPNPIIRPSIAKVLQMSEGDFEVSVPPLFDGKIL*