AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017134


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017134 MtrunA17_Chr3g0136701 97.586 290 7 0 1 290 1 290 0.0 590
MsaG017134 MtrunA17_Chr1g0150471 54.403 318 115 7 1 290 1 316 3.82e-106 311
MsaG017134 MtrunA17_Chr4g0072871 51.205 332 101 11 4 280 20 345 4.65e-89 270
MsaG017134 MtrunA17_Chr7g0220721 63.043 92 34 0 3 94 23 114 1.31e-37 137
MsaG017134 MtrunA17_Chr7g0220721 71.429 70 9 2 231 289 326 395 4.99e-23 97.4
MsaG017134 MtrunA17_Chr5g0405151 50.602 83 33 1 5 79 4 86 1.06e-20 87.4
MsaG017134 MtrunA17_Chr5g0429341 43.333 90 51 0 5 94 4 93 2.51e-19 87.4
MsaG017134 MtrunA17_Chr1g0155811 43.299 97 45 1 6 92 5 101 4.99e-19 84.7
MsaG017134 MtrunA17_Chr2g0314671 44.792 96 44 1 6 92 5 100 1.08e-18 84.0
MsaG017134 MtrunA17_Chr4g0033641 43.750 96 45 1 6 92 5 100 1.10e-18 83.2
MsaG017134 MtrunA17_Chr2g0329511 42.708 96 46 1 6 92 5 100 1.56e-17 80.1
MsaG017134 MtrunA17_Chr4g0035651 39.175 97 49 1 6 92 5 101 5.64e-17 78.2
MsaG017134 MtrunA17_Chr3g0145061 38.144 97 50 1 6 92 5 101 6.44e-17 78.2
MsaG017134 MtrunA17_Chr7g0268691 39.326 89 53 1 6 94 20 107 3.01e-16 78.2
MsaG017134 MtrunA17_Chr3g0120711 39.216 102 61 1 5 106 4 104 3.82e-16 78.2
MsaG017134 MtrunA17_Chr3g0120711 44.872 78 40 1 202 279 327 401 3.16e-11 63.5
MsaG017134 MtrunA17_Chr2g0322941 45.122 82 45 0 5 86 43 124 3.12e-15 73.6
MsaG017134 MtrunA17_Chr3g0142221 37.895 95 48 1 6 89 5 99 8.82e-15 72.0
MsaG017134 MtrunA17_Chr7g0251781 44.444 72 40 0 6 77 5 76 1.33e-13 70.5
MsaG017134 MtrunA17_Chr2g0322951 38.318 107 46 2 189 279 37 139 1.25e-12 64.3
MsaG017134 MtrunA17_Chr1g0209071 39.189 74 45 0 4 77 3 76 1.53e-12 67.0
MsaG017134 MtrunA17_Chr1g0209861 61.818 55 17 1 224 274 370 424 3.06e-12 66.6
MsaG017134 MtrunA17_Chr8g0390971 70.455 44 13 0 232 275 358 401 4.87e-12 65.9
MsaG017134 MtrunA17_Chr5g0431351 66.667 45 15 0 230 274 172 216 7.14e-12 63.5
MsaG017134 MtrunA17_Chr3g0127941 49.231 65 32 1 219 283 617 680 7.36e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017134 AT5G24930.1 52.000 375 80 13 1 289 1 361 6.65e-95 285
MsaG017134 AT2G24790.1 54.266 293 95 11 2 277 4 274 4.73e-94 280
MsaG017134 AT5G57660.1 42.775 346 131 10 2 289 18 354 2.34e-74 232
MsaG017134 AT3G02380.1 40.351 342 141 9 3 289 13 346 4.42e-73 228
MsaG017134 AT5G15850.1 41.210 347 143 8 3 289 9 354 5.84e-70 220
MsaG017134 AT5G15840.1 39.335 361 134 14 6 289 20 372 1.29e-65 210
MsaG017134 AT2G24790.2 56.180 178 55 6 2 171 4 166 8.17e-58 185
MsaG017134 AT5G15840.2 41.935 186 82 7 6 166 20 204 2.12e-37 134
MsaG017134 AT1G49130.1 28.435 313 176 10 4 280 26 326 1.65e-22 95.5
MsaG017134 AT1G49130.2 28.435 313 176 10 4 280 19 319 1.85e-22 95.5
MsaG017134 AT4G38960.1 35.570 149 74 3 5 138 4 145 3.41e-20 86.3
MsaG017134 AT4G38960.6 35.570 149 74 3 5 138 4 145 3.41e-20 86.3
MsaG017134 AT2G21320.1 49.398 83 34 1 5 79 4 86 6.48e-20 85.1
MsaG017134 AT1G78600.2 42.017 119 56 3 6 111 5 123 4.97e-19 85.9
MsaG017134 AT1G78600.1 42.017 119 56 3 6 111 5 123 5.28e-19 85.5
MsaG017134 AT2G47890.3 42.857 91 50 1 4 94 11 99 4.59e-18 82.8
MsaG017134 AT2G47890.1 42.857 91 50 1 4 94 11 99 5.13e-18 83.2
MsaG017134 AT2G47890.1 27.273 308 161 14 3 276 53 331 1.35e-13 70.5
MsaG017134 AT2G47890.2 42.857 91 50 1 4 94 11 99 6.31e-18 81.3
MsaG017134 AT1G06040.1 43.750 96 45 1 6 92 5 100 7.36e-18 81.3
MsaG017134 AT1G06040.2 43.750 96 45 1 6 92 5 100 1.01e-17 79.3
MsaG017134 AT1G75540.1 40.404 99 47 1 6 92 5 103 1.17e-17 82.0
MsaG017134 AT2G33500.1 42.697 89 51 0 6 94 12 100 3.25e-17 81.6
MsaG017134 AT2G33500.1 52.632 57 27 0 220 276 345 401 1.09e-12 68.2
MsaG017134 AT2G33500.2 42.697 89 51 0 6 94 12 100 3.26e-17 81.6
MsaG017134 AT2G33500.2 52.632 57 27 0 220 276 344 400 1.12e-12 68.2
MsaG017134 AT2G31380.1 34.177 158 87 4 6 153 5 155 1.44e-16 77.8
MsaG017134 AT1G28050.1 41.573 89 52 0 6 94 9 97 4.62e-16 78.2
MsaG017134 AT1G28050.1 56.000 50 22 0 228 277 381 430 9.23e-11 62.4
MsaG017134 AT5G48250.1 38.043 92 55 1 5 94 4 95 1.11e-14 73.9
MsaG017134 AT3G07650.3 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
MsaG017134 AT3G07650.3 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
MsaG017134 AT3G07650.2 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
MsaG017134 AT3G07650.2 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
MsaG017134 AT3G07650.4 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
MsaG017134 AT3G07650.4 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
MsaG017134 AT3G07650.1 45.205 73 40 0 5 77 4 76 3.37e-14 72.4
MsaG017134 AT3G07650.1 35.185 108 65 2 174 279 258 362 8.73e-11 62.4
MsaG017134 AT4G10240.1 33.714 175 81 7 6 165 5 159 4.33e-14 68.9
MsaG017134 AT4G39070.1 37.755 98 51 1 5 92 4 101 1.71e-13 68.9
MsaG017134 AT1G73870.1 55.556 63 27 1 213 275 327 388 2.52e-13 70.1
MsaG017134 AT4G15250.1 40.476 84 50 0 6 89 5 88 4.70e-13 68.9
MsaG017134 AT1G68190.2 38.824 85 50 1 4 88 41 123 6.48e-13 68.9
MsaG017134 AT1G68190.1 38.824 85 50 1 4 88 12 94 7.72e-13 68.6
MsaG017134 AT1G68190.3 38.824 85 50 1 4 88 12 94 7.72e-13 68.6
MsaG017134 AT1G25440.1 36.029 136 72 5 158 286 288 415 4.88e-12 66.2
MsaG017134 AT3G21880.1 38.202 89 53 1 6 94 5 91 1.29e-11 64.7
MsaG017134 AT3G21880.3 38.202 89 53 1 6 94 9 95 1.41e-11 64.7
MsaG017134 AT3G21880.2 38.202 89 53 1 6 94 9 95 1.41e-11 64.7
MsaG017134 AT5G14370.1 63.043 46 17 0 232 277 294 339 1.94e-11 64.3
MsaG017134 AT4G25990.1 60.870 46 18 0 232 277 341 386 2.43e-11 63.9
MsaG017134 AT1G68520.1 68.182 44 14 0 232 275 357 400 2.60e-11 63.9
MsaG017134 AT5G60100.2 31.217 189 106 6 95 276 342 513 2.99e-11 63.9
MsaG017134 AT5G60100.5 31.217 189 106 6 95 276 315 486 3.42e-11 63.9
MsaG017134 AT5G60100.3 31.217 189 106 6 95 276 315 486 3.42e-11 63.9
MsaG017134 AT5G60100.1 31.217 189 106 6 95 276 315 486 3.42e-11 63.9

Find 71 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCAGTTGATTCCATTTC+TGG 0.107062 3:+106077231 MsaT017134.1:CDS
GAAACTAGGCCTAGGATTAA+AGG 0.280004 3:+106077189 MsaT017134.1:CDS
CCGCAGAGGAAGGCGGAGTC+AGG 0.296878 3:-106076357 None:intergenic
TTCCGAAGCTATTCCGTTCA+TGG 0.298068 3:+106076728 MsaT017134.1:CDS
AAGCTAGGGTTATGAGGTAT+AGG 0.350898 3:+106077103 MsaT017134.1:CDS
GACGGTGTTGTTCCCGTTCA+TGG 0.369225 3:+106076783 MsaT017134.1:CDS
GCAAACCTCCCTTTAATCCT+AGG 0.392261 3:-106077198 None:intergenic
CCGTTCATGGATCTCGATTA+CGG 0.395344 3:+106076741 MsaT017134.1:CDS
TTTGTTAGCGGCGTGGACTT+TGG 0.416071 3:-106076391 None:intergenic
GGATCGAAGTTGCCATGAAC+GGG 0.430806 3:-106076795 None:intergenic
TGGCGGCGGATCGTGAAGCT+AGG 0.432649 3:+106077088 MsaT017134.1:CDS
GATTCGAAGAACGGAGTAAC+GGG 0.439450 3:-106076567 None:intergenic
AGCTAGGGTTATGAGGTATA+GGG 0.439658 3:+106077104 MsaT017134.1:CDS
AGATATCGAACTGTGGTTAC+GGG 0.444139 3:+106077061 MsaT017134.1:CDS
GAGATATCGAACTGTGGTTA+CGG 0.445942 3:+106077060 MsaT017134.1:CDS
AACGATGCCGTATCCAGAAA+TGG 0.448783 3:-106077244 None:intergenic
GTTGATTCCATTTCTGGATA+CGG 0.451790 3:+106077237 MsaT017134.1:CDS
GATGAGTTGAGATCTGCTTT+CGG 0.461772 3:-106076687 None:intergenic
TCCGACACGGTGTTCCCGTC+AGG 0.479338 3:-106077039 None:intergenic
TTTAACAGAGTGAGAAGTGT+TGG 0.480554 3:-106076589 None:intergenic
TGGTTACGGGACGGTGGCGG+CGG 0.488159 3:+106077074 MsaT017134.1:CDS
AAACTAGGCCTAGGATTAAA+GGG 0.490422 3:+106077190 MsaT017134.1:CDS
AGTGTGGATGTCACGGTCAC+AGG 0.492186 3:-106076511 None:intergenic
ATCCATGAACGGAATAGCTT+CGG 0.492715 3:-106076730 None:intergenic
TTATGAGGTATAGGGAGAAG+AGG 0.496368 3:+106077112 MsaT017134.1:CDS
TTCTTGTAAGCTGAAACAAA+TGG 0.496560 3:-106076816 None:intergenic
TTAGGTATCGTCTTCGTCGA+TGG 0.503016 3:+106077002 MsaT017134.1:intron
GTAAAGCATATGCGGAAACT+AGG 0.510714 3:+106077175 MsaT017134.1:CDS
CTGTGGTTACGGGACGGTGG+CGG 0.513006 3:+106077071 MsaT017134.1:CDS
CGCAGAGGAAGGCGGAGTCA+GGG 0.518288 3:-106076356 None:intergenic
TCGTCTTCGTCGATGGAGGT+TGG 0.523017 3:+106077009 MsaT017134.1:CDS
AGGTTGGAGTTGTTCCTGAC+GGG 0.524585 3:+106077025 MsaT017134.1:CDS
GCTTTACACGTGACATGAGC+AGG 0.534087 3:-106076465 None:intergenic
GGATTCGAAGAACGGAGTAA+CGG 0.536432 3:-106076568 None:intergenic
GGCGTGGACTTTGGAGTCAC+AGG 0.543388 3:-106076382 None:intergenic
GTCACAGGCGCCGCAGAGGA+AGG 0.545693 3:-106076367 None:intergenic
TCGGAATCAGAGAAAAGATA+AGG 0.547155 3:-106076711 None:intergenic
TGGATCGAAGTTGCCATGAA+CGG 0.558189 3:-106076796 None:intergenic
CGCTCGTGACGTGCGGCGAG+AGG 0.558417 3:-106076540 None:intergenic
ATGAAGCTGAAGTTGCTTCG+TGG 0.559796 3:+106076652 MsaT017134.1:CDS
GCAGTAGAGAGTAGCGGTGG+CGG 0.563031 3:-106076331 None:intergenic
GAAGTGTTGGATTCGAAGAA+CGG 0.565969 3:-106076576 None:intergenic
GATGCAGAGAGAAGCTGCAT+CGG 0.576026 3:-106076487 None:intergenic
CATATGCGGAAACTAGGCCT+AGG 0.577094 3:+106077181 MsaT017134.1:CDS
GGCGGCGGATCGTGAAGCTA+GGG 0.577274 3:+106077089 MsaT017134.1:CDS
ACGAGAGGCGAGTTTGTTAG+CGG 0.583367 3:-106076403 None:intergenic
GGTATCGTCTTCGTCGATGG+AGG 0.585063 3:+106077005 MsaT017134.1:intron
ACAGGCGCCGCAGAGGAAGG+CGG 0.586506 3:-106076364 None:intergenic
GATTAGCAGTGTGGATGTCA+CGG 0.589251 3:-106076518 None:intergenic
CGCTTCTCGTAAAGCATATG+CGG 0.592961 3:+106077167 MsaT017134.1:CDS
GAGGTTGGAGTTGTTCCTGA+CGG 0.607148 3:+106077024 MsaT017134.1:CDS
GGGAACACGCTCGTGACGTG+CGG 0.610507 3:-106076547 None:intergenic
ATCGTGAAGCTAGGGTTATG+AGG 0.615678 3:+106077097 MsaT017134.1:CDS
TGGAGTCACAGGCGCCGCAG+AGG 0.616423 3:-106076371 None:intergenic
AGGCGAGTTTGTTAGCGGCG+TGG 0.630051 3:-106076398 None:intergenic
TGACTAATCTGCGACTGTGA+CGG 0.630286 3:-106076873 None:intergenic
AGTGACGCGAGGGTGACGAG+AGG 0.632213 3:-106076418 None:intergenic
GCGGCAGTAGAGAGTAGCGG+TGG 0.638763 3:-106076334 None:intergenic
AGGGCGGCAGTAGAGAGTAG+CGG 0.646899 3:-106076337 None:intergenic
CGGCGAGAGGATTAGCAGTG+TGG 0.659805 3:-106076527 None:intergenic
AGAGGAAGGCGGAGTCAGGG+CGG 0.663446 3:-106076353 None:intergenic
CCTGACTCCGCCTTCCTCTG+CGG 0.671751 3:+106076357 MsaT017134.1:CDS
ATCGAACTGTGGTTACGGGA+CGG 0.672790 3:+106077065 MsaT017134.1:CDS
TCCTGACGGGAACACCGTGT+CGG 0.682213 3:+106077038 MsaT017134.1:CDS
GAACTGTGGTTACGGGACGG+TGG 0.687113 3:+106077068 MsaT017134.1:CDS
CTTCGCAGAGAGTGACGCGA+GGG 0.694593 3:-106076428 None:intergenic
CTAGGCCTAGGATTAAAGGG+AGG 0.700310 3:+106077193 MsaT017134.1:CDS
ACTTCGCAGAGAGTGACGCG+AGG 0.710854 3:-106076429 None:intergenic
CCGTAATCGAGATCCATGAA+CGG 0.730276 3:-106076741 None:intergenic
ACAGTTCGATATCTCCGACA+CGG 0.744977 3:-106077052 None:intergenic
GTGTCGGAGATATCGAACTG+TGG 0.760977 3:+106077054 MsaT017134.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 106076300 106077278 106076300 ID=MsaG017134
Chr3 mRNA 106076300 106077278 106076300 ID=MsaT017134.1;Parent=MsaG017134
Chr3 exon 106076300 106076899 106076300 ID=MsaT017134.1.exon1;Parent=MsaT017134.1
Chr3 CDS 106076300 106076899 106076300 ID=cds.MsaT017134.1;Parent=MsaT017134.1
Chr3 exon 106077006 106077278 106077006 ID=MsaT017134.1.exon2;Parent=MsaT017134.1
Chr3 CDS 106077006 106077278 106077006 ID=cds.MsaT017134.1;Parent=MsaT017134.1
Gene Sequence

>MsaT017134.1

ATGGCTTCTAAACTCTGTGACTCATGCAAATCCGCCACCGCTACTCTCTACTGCCGCCCTGACTCCGCCTTCCTCTGCGGCGCCTGTGACTCCAAAGTCCACGCCGCTAACAAACTCGCCTCTCGTCACCCTCGCGTCACTCTCTGCGAAGTCTGCGAACAAGCACCTGCTCATGTCACGTGTAAAGCCGATGCAGCTTCTCTCTGCATCACCTGTGACCGTGACATCCACACTGCTAATCCTCTCGCCGCACGTCACGAGCGTGTTCCCGTTACTCCGTTCTTCGAATCCAACACTTCTCACTCTGTTAAATCGCTTCAAAACAATAACAACAACTACGATGCTGTTAAAGATGAAGCTGAAGTTGCTTCGTGGTTGATCTCTGATCCGAAAGCAGATCTCAACTCATCTCCTTATCTTTTCTCTGATTCCGAAGCTATTCCGTTCATGGATCTCGATTACGGTGTAATTGAACATAAAAACGACGGTGTTGTTCCCGTTCATGGCAACTTCGATCCATTTGTTTCAGCTTACAAGAACAACAACGTGCACTTGCATACAGAGTTAGAAACACCGTCACAGTCGCAGATTAGTCAGAGTGTATCGTCTTCGTCGATGGAGGTTGGAGTTGTTCCTGACGGGAACACCGTGTCGGAGATATCGAACTGTGGTTACGGGACGGTGGCGGCGGATCGTGAAGCTAGGGTTATGAGGTATAGGGAGAAGAGGAAGAATAGAAGATTCGAGAAGACGATTCGTTACGCTTCTCGTAAAGCATATGCGGAAACTAGGCCTAGGATTAAAGGGAGGTTTGCAAAACGCACTGATGCAGTTGATTCCATTTCTGGATACGGCATCGTTCCCACTTGTTGA

Protein sequence

>MsaT017134.1

MASKLCDSCKSATATLYCRPDSAFLCGACDSKVHAANKLASRHPRVTLCEVCEQAPAHVTCKADAASLCITCDRDIHTANPLAARHERVPVTPFFESNTSHSVKSLQNNNNNYDAVKDEAEVASWLISDPKADLNSSPYLFSDSEAIPFMDLDYGVIEHKNDGVVPVHGNFDPFVSAYKNNNVHLHTELETPSQSQISQSVSSSSMEVGVVPDGNTVSEISNCGYGTVAADREARVMRYREKRKNRRFEKTIRYASRKAYAETRPRIKGRFAKRTDAVDSISGYGIVPTC*