Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017165 | XP_003603313.1 | 84.946 | 186 | 21 | 3 | 1 | 186 | 1 | 179 | 1.16e-102 | 305 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017165 | sp|Q39262|ZFP3_ARATH | 45.385 | 130 | 46 | 7 | 48 | 176 | 37 | 142 | 5.52e-22 | 92.0 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017165 | tr|G7J9W4|G7J9W4_MEDTR | 84.946 | 186 | 21 | 3 | 1 | 186 | 1 | 179 | 5.55e-103 | 305 |
Gene ID | Type | Classification |
---|---|---|
MsaG017165 | TF | C2H2 |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000306 | MsaG017165 | 0.830548 | 4.966685e-55 | 1.460039e-52 |
MsaG000359 | MsaG017165 | 0.815610 | 1.466361e-51 | 2.878785e-49 |
MsaG001169 | MsaG017165 | 0.807912 | 6.819919e-50 | 1.107529e-47 |
MsaG001724 | MsaG017165 | 0.808508 | 5.097584e-50 | 8.397535e-48 |
MsaG002350 | MsaG017165 | 0.805498 | 2.193728e-49 | 3.364391e-47 |
MsaG002604 | MsaG017165 | 0.807795 | 7.217510e-50 | 1.168797e-47 |
MsaG003102 | MsaG017165 | 0.803476 | 5.763074e-49 | 8.433929e-47 |
MsaG004101 | MsaG017165 | 0.818069 | 4.141803e-52 | 8.660274e-50 |
MsaG004146 | MsaG017165 | 0.813798 | 3.678376e-51 | 6.899323e-49 |
MsaG004648 | MsaG017165 | 0.811325 | 1.270329e-50 | 2.240742e-48 |
MsaG004656 | MsaG017165 | 0.818302 | 3.668663e-52 | 7.717183e-50 |
MsaG004913 | MsaG017165 | 0.804791 | 3.078780e-49 | 4.645035e-47 |
MsaG005092 | MsaG017165 | 0.807294 | 9.211591e-50 | 1.473876e-47 |
MsaG005880 | MsaG017165 | 0.812425 | 7.336203e-51 | 1.329561e-48 |
MsaG006177 | MsaG017165 | 0.831687 | 2.616028e-55 | 7.948903e-53 |
MsaG006837 | MsaG017165 | 0.805790 | 1.906337e-49 | 2.943777e-47 |
MsaG006898 | MsaG017165 | 0.809030 | 3.946078e-50 | 6.582965e-48 |
MsaG006886 | MsaG017165 | 0.821564 | 6.638804e-53 | 1.521828e-50 |
MsaG007072 | MsaG017165 | 0.800468 | 2.376438e-48 | 3.247256e-46 |
MsaG009060 | MsaG017165 | 0.833297 | 1.048230e-55 | 3.338078e-53 |
MsaG009764 | MsaG017165 | 0.808492 | 5.137567e-50 | 8.460221e-48 |
MsaG010328 | MsaG017165 | 0.810428 | 1.981690e-50 | 3.419820e-48 |
MsaG010372 | MsaG017165 | 0.824318 | 1.525115e-53 | 3.765852e-51 |
MsaG011285 | MsaG017165 | 0.836858 | 1.339571e-56 | 4.744529e-54 |
MsaG011913 | MsaG017165 | 0.809246 | 3.550500e-50 | 5.953616e-48 |
MsaG011952 | MsaG017165 | 0.812530 | 6.958911e-51 | 1.264498e-48 |
MsaG012778 | MsaG017165 | 0.802603 | 8.715257e-49 | 1.250065e-46 |
MsaG014950 | MsaG017165 | 0.809855 | 2.630901e-50 | 4.477131e-48 |
MsaG015143 | MsaG017165 | 0.812989 | 5.528961e-51 | 1.016190e-48 |
MsaG015446 | MsaG017165 | 0.821186 | 8.108698e-53 | 1.840302e-50 |
MsaG015807 | MsaG017165 | 0.819238 | 2.254099e-52 | 4.859065e-50 |
MsaG015809 | MsaG017165 | 0.828341 | 1.697319e-54 | 4.686350e-52 |
MsaG015851 | MsaG017165 | 0.808030 | 6.437960e-50 | 1.048462e-47 |
MsaG016163 | MsaG017165 | 0.814863 | 2.145194e-51 | 4.132529e-49 |
MsaG016421 | MsaG017165 | 0.815093 | 1.908486e-51 | 3.697834e-49 |
MsaG017105 | MsaG017165 | 0.808630 | 4.802093e-50 | 7.934064e-48 |
MsaG017165 | MsaG017183 | 0.801807 | 1.268593e-48 | 1.786788e-46 |
MsaG017165 | MsaG017188 | 0.802494 | 9.176999e-49 | 1.312993e-46 |
MsaG017165 | MsaG017690 | 0.809565 | 3.034089e-50 | 5.127432e-48 |
MsaG017165 | MsaG017839 | 0.822661 | 3.705814e-53 | 8.748237e-51 |
MsaG017165 | MsaG017907 | 0.809227 | 3.582598e-50 | 6.004718e-48 |
MsaG017165 | MsaG019092 | 0.812407 | 7.402204e-51 | 1.340937e-48 |
MsaG017165 | MsaG019132 | 0.835112 | 3.695841e-56 | 1.242124e-53 |
MsaG017165 | MsaG020353 | 0.806533 | 1.331529e-49 | 2.092667e-47 |
MsaG017165 | MsaG021516 | 0.808896 | 4.214496e-50 | 7.008174e-48 |
MsaG017165 | MsaG023415 | 0.829964 | 6.890476e-55 | 1.992127e-52 |
MsaG017165 | MsaG023570 | 0.801269 | 1.633354e-48 | 2.272535e-46 |
MsaG017165 | MsaG023885 | 0.800360 | 2.499582e-48 | 3.407191e-46 |
MsaG017165 | MsaG024293 | 0.807964 | 6.647719e-50 | 1.080897e-47 |
MsaG017165 | MsaG025492 | 0.829408 | 9.391091e-55 | 2.672084e-52 |
MsaG017165 | MsaG025732 | 0.811861 | 9.722939e-51 | 1.737764e-48 |
MsaG017165 | MsaG026579 | 0.803953 | 4.593453e-49 | 6.796998e-47 |
MsaG017165 | MsaG027159 | 0.800832 | 2.004447e-48 | 2.761521e-46 |
MsaG017165 | MsaG028761 | 0.805297 | 2.415649e-49 | 3.687593e-47 |
MsaG017165 | MsaG028935 | 0.814825 | 2.187163e-51 | 4.209382e-49 |
MsaG017165 | MsaG029117 | 0.814836 | 2.174422e-51 | 4.186033e-49 |
MsaG017165 | MsaG029694 | 0.814625 | 2.420086e-51 | 4.634146e-49 |
MsaG017165 | MsaG031201 | 0.816660 | 8.566602e-52 | 1.727315e-49 |
MsaG017165 | MsaG031652 | 0.805731 | 1.960541e-49 | 3.023350e-47 |
MsaG017165 | MsaG032133 | 0.812497 | 7.076740e-51 | 1.284850e-48 |
MsaG017165 | MsaG032228 | 0.825400 | 8.498527e-54 | 2.161388e-51 |
MsaG017165 | MsaG033688 | 0.803221 | 6.504766e-49 | 9.463524e-47 |
MsaG017165 | MsaG033700 | 0.832018 | 2.170211e-55 | 6.658015e-53 |
MsaG017165 | MsaG033815 | 0.811286 | 1.294781e-50 | 2.281714e-48 |
MsaG017165 | MsaG033922 | 0.805222 | 2.504121e-49 | 3.816036e-47 |
MsaG017165 | MsaG034285 | 0.819308 | 2.173629e-52 | 4.694240e-50 |
MsaG017165 | MsaG034673 | 0.808539 | 5.020318e-50 | 8.276606e-48 |
MsaG017165 | MsaG035513 | 0.810340 | 2.070025e-50 | 3.564572e-48 |
MsaG017165 | MsaG040381 | 0.811376 | 1.238435e-50 | 2.187188e-48 |
MsaG017165 | MsaG040474 | 0.809384 | 3.317389e-50 | 5.581371e-48 |
MsaG017165 | MsaG040517 | 0.819366 | 2.108775e-52 | 4.561198e-50 |
MsaG017165 | MsaG041224 | 0.802462 | 9.319313e-49 | 1.332352e-46 |
MsaG017165 | MsaG042791 | 0.803267 | 6.364348e-49 | 9.268971e-47 |
MsaG017165 | MsaG044048 | 0.832292 | 1.857288e-55 | 5.743120e-53 |
MsaG017165 | MsaG044623 | 0.821396 | 7.257823e-53 | 1.656299e-50 |
MsaG017165 | MsaG044880 | 0.806689 | 1.234805e-49 | 1.947807e-47 |
MsaG017165 | MsaG045060 | 0.823855 | 1.956701e-53 | 4.770795e-51 |
MsaG017165 | MsaG045107 | 0.830038 | 6.608574e-55 | 1.914691e-52 |
MsaG017165 | MsaG045200 | 0.809232 | 3.573689e-50 | 5.990538e-48 |
MsaG017165 | MsaG045997 | 0.801661 | 1.358960e-48 | 1.907700e-46 |
MsaG017165 | MsaG046105 | 0.806769 | 1.188345e-49 | 1.878004e-47 |
MsaG017165 | MsaG046113 | 0.824464 | 1.409462e-53 | 3.494046e-51 |
MsaG017165 | MsaG047073 | 0.807758 | 7.351249e-50 | 1.189363e-47 |
MsaG017165 | MsaG004537 | 0.837590 | 8.717319e-57 | 3.157223e-54 |
MsaG017165 | MsaG004304 | 0.815157 | 1.847566e-51 | 3.585610e-49 |
MsaG017165 | MsaG008983 | 0.810352 | 2.057636e-50 | 3.544262e-48 |
MsaG017165 | MsaG013007 | 0.816404 | 9.770557e-52 | 1.957222e-49 |
MsaG017165 | MsaG013379 | 0.838857 | 4.127849e-57 | 1.554893e-54 |
MsaG017165 | MsaG011352 | 0.811586 | 1.115185e-50 | 1.979707e-48 |
MsaG017165 | MsaG013700 | 0.812723 | 6.317210e-51 | 1.153388e-48 |
MsaG017165 | MsaG021945 | 0.802460 | 9.327753e-49 | 1.333510e-46 |
MsaG017165 | MsaG032120 | 0.823020 | 3.059673e-53 | 7.293419e-51 |
MsaG017165 | MsaG033077 | 0.811883 | 9.618109e-51 | 1.719954e-48 |
MsaG017165 | MsaG030190 | 0.805562 | 2.127375e-49 | 3.267514e-47 |
MsaG017165 | MsaG033058 | 0.802000 | 1.158439e-48 | 1.638768e-46 |
MsaG017165 | MsaG037513 | 0.802815 | 7.886889e-49 | 1.136704e-46 |
MsaG017165 | MsaG036287 | 0.807219 | 9.551732e-50 | 1.525592e-47 |
MsaG017165 | MsaG035918 | 0.808874 | 4.259620e-50 | 7.079534e-48 |
MsaG017165 | MsaG035299 | 0.800389 | 2.465942e-48 | 3.363543e-46 |
MsaG017165 | MsaG045457 | 0.801234 | 1.660769e-48 | 2.308813e-46 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017165 | MtrunA17_Chr3g0137111 | 84.946 | 186 | 21 | 3 | 1 | 186 | 1 | 179 | 1.07e-106 | 305 |
MsaG017165 | MtrunA17_Chr1g0149811 | 51.872 | 187 | 70 | 9 | 5 | 185 | 24 | 196 | 7.97e-48 | 156 |
MsaG017165 | MtrunA17_Chr2g0333741 | 42.857 | 175 | 76 | 10 | 12 | 183 | 20 | 173 | 1.07e-26 | 101 |
MsaG017165 | MtrunA17_Chr1g0161901 | 44.068 | 118 | 42 | 6 | 54 | 165 | 69 | 168 | 1.86e-19 | 82.8 |
MsaG017165 | MtrunA17_Chr5g0434121 | 52.874 | 87 | 21 | 3 | 76 | 161 | 86 | 153 | 1.03e-18 | 80.5 |
MsaG017165 | MtrunA17_Chr4g0001081 | 43.810 | 105 | 29 | 3 | 75 | 174 | 65 | 144 | 1.14e-18 | 80.1 |
MsaG017165 | MtrunA17_Chr8g0376021 | 48.352 | 91 | 27 | 4 | 76 | 165 | 61 | 132 | 1.20e-18 | 80.1 |
MsaG017165 | MtrunA17_Chr3g0110091 | 48.913 | 92 | 26 | 4 | 77 | 167 | 86 | 157 | 1.15e-17 | 77.8 |
MsaG017165 | MtrunA17_Chr1g0207691 | 40.206 | 97 | 36 | 3 | 71 | 166 | 57 | 132 | 3.88e-15 | 70.1 |
MsaG017165 | MtrunA17_Chr4g0072571 | 73.684 | 38 | 10 | 0 | 75 | 112 | 43 | 80 | 1.87e-14 | 67.8 |
MsaG017165 | MtrunA17_Chr3g0081121 | 42.857 | 98 | 47 | 5 | 69 | 166 | 90 | 178 | 4.21e-14 | 68.9 |
MsaG017165 | MtrunA17_Chr7g0250871 | 42.529 | 87 | 39 | 3 | 57 | 143 | 33 | 108 | 1.45e-11 | 60.1 |
MsaG017165 | MtrunA17_Chr3g0079671 | 35.398 | 113 | 52 | 4 | 74 | 176 | 95 | 196 | 1.53e-11 | 61.6 |
MsaG017165 | MtrunA17_Chr3g0081131 | 36.364 | 110 | 50 | 4 | 71 | 176 | 92 | 185 | 2.40e-11 | 61.2 |
MsaG017165 | MtrunA17_Chr7g0222661 | 33.613 | 119 | 59 | 6 | 71 | 184 | 80 | 183 | 3.74e-11 | 60.5 |
MsaG017165 | MtrunA17_Chr7g0254221 | 61.538 | 39 | 15 | 0 | 77 | 115 | 33 | 71 | 4.49e-11 | 58.5 |
MsaG017165 | MtrunA17_Chr5g0396031 | 38.889 | 90 | 43 | 3 | 77 | 166 | 46 | 123 | 7.12e-11 | 60.1 |
MsaG017165 | MtrunA17_Chr7g0251581 | 69.697 | 33 | 10 | 0 | 77 | 109 | 39 | 71 | 8.55e-11 | 58.2 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017165 | AT5G25160.1 | 45.385 | 130 | 46 | 7 | 48 | 176 | 37 | 142 | 5.61e-23 | 92.0 |
MsaG017165 | AT1G80730.1 | 40.876 | 137 | 72 | 4 | 37 | 166 | 20 | 154 | 1.52e-22 | 90.9 |
MsaG017165 | AT5G10970.1 | 37.333 | 150 | 72 | 5 | 18 | 166 | 48 | 176 | 1.01e-20 | 87.0 |
MsaG017165 | AT1G66140.1 | 46.739 | 92 | 37 | 3 | 77 | 161 | 83 | 169 | 2.28e-16 | 75.1 |
MsaG017165 | AT1G24625.1 | 76.316 | 38 | 9 | 0 | 74 | 111 | 54 | 91 | 1.33e-15 | 72.0 |
MsaG017165 | AT5G57520.1 | 65.909 | 44 | 15 | 0 | 75 | 118 | 48 | 91 | 6.39e-15 | 68.9 |
MsaG017165 | AT5G14010.1 | 73.529 | 34 | 9 | 0 | 77 | 110 | 36 | 69 | 8.83e-13 | 63.5 |
MsaG017165 | AT1G67030.1 | 44.944 | 89 | 44 | 3 | 44 | 132 | 7 | 90 | 2.20e-11 | 60.5 |
MsaG017165 | AT3G58070.1 | 52.459 | 61 | 24 | 1 | 56 | 116 | 73 | 128 | 4.37e-11 | 60.5 |
Find 30 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TATGAGACCATTTGCAATTA+AGG | 0.217801 | 3:+106411192 | MsaT017165.1:CDS |
GGAATTGAAAGCAATTATTA+TGG | 0.251312 | 3:+106411265 | MsaT017165.1:CDS |
TCACAACCGTTATGCAAATA+TGG | 0.314680 | 3:+106411132 | MsaT017165.1:CDS |
CCTAACGATGATTGTGATCT+TGG | 0.347148 | 3:+106410842 | MsaT017165.1:CDS |
ATTGCAGAATCCACATGAAT+TGG | 0.369569 | 3:+106410751 | MsaT017165.1:CDS |
AGCAATTATTATGGATATCA+TGG | 0.375634 | 3:+106411274 | MsaT017165.1:CDS |
AGAATTATCTATGTCTAAAC+AGG | 0.387114 | 3:-106410891 | None:intergenic |
TTGGATGAAAATTTATGAAA+AGG | 0.404264 | 3:-106411235 | None:intergenic |
GGAGAAACATAAAACCTTTG+AGG | 0.452533 | 3:+106410772 | MsaT017165.1:CDS |
AACCTTTGAGGAAGAAGAAA+AGG | 0.455284 | 3:+106410784 | MsaT017165.1:CDS |
AGAACGATGTTCTCAGCAAC+AGG | 0.459446 | 3:+106411076 | MsaT017165.1:CDS |
TTCTTCAGTTCACAAGCACT+TGG | 0.470251 | 3:+106411001 | MsaT017165.1:CDS |
CTAACGATGATTGTGATCTT+GGG | 0.483992 | 3:+106410843 | MsaT017165.1:CDS |
GCTTTCAATTCCAAAGCCAT+TGG | 0.496119 | 3:-106411254 | None:intergenic |
TCTTCAGTTCACAAGCACTT+GGG | 0.508835 | 3:+106411002 | MsaT017165.1:CDS |
CTTCAGTTCACAAGCACTTG+GGG | 0.514360 | 3:+106411003 | MsaT017165.1:CDS |
GAGTGTGCCTTAATTGCAAA+TGG | 0.520506 | 3:-106411199 | None:intergenic |
TGCAATTAAGGCACACTCGA+TGG | 0.523345 | 3:+106411204 | MsaT017165.1:CDS |
CCAAGATCACAATCATCGTT+AGG | 0.527605 | 3:-106410842 | None:intergenic |
GGAAGAAGAAAAGGAGGCAA+AGG | 0.531006 | 3:+106410793 | MsaT017165.1:CDS |
TTATGTTTCTCCAATTCATG+TGG | 0.532950 | 3:-106410761 | None:intergenic |
ATGATATTTATGAAGAAATG+AGG | 0.554191 | 3:-106411107 | None:intergenic |
GAGATGCCATATTTGCATAA+CGG | 0.557005 | 3:-106411138 | None:intergenic |
TCATCAGAAAACTTCACTTG+TGG | 0.611490 | 3:+106410923 | MsaT017165.1:CDS |
AAAATGCTCACAAGAGAGAG+AGG | 0.643637 | 3:+106411032 | MsaT017165.1:CDS |
TTCAGTTCACAAGCACTTGG+GGG | 0.644584 | 3:+106411004 | MsaT017165.1:CDS |
CTTTGAGGAAGAAGAAAAGG+AGG | 0.655746 | 3:+106410787 | MsaT017165.1:CDS |
ATATTTATGAAGAAATGAGG+TGG | 0.662714 | 3:-106411104 | None:intergenic |
TTACTATTAGCACAGAAGAG+TGG | 0.684083 | 3:-106411166 | None:intergenic |
ACGATGTTCTCAGCAACAGG+AGG | 0.689396 | 3:+106411079 | MsaT017165.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr3 | gene | 106410740 | 106411300 | 106410740 | ID=MsaG017165 |
Chr3 | mRNA | 106410740 | 106411300 | 106410740 | ID=MsaT017165.1;Parent=MsaG017165 |
Chr3 | exon | 106410740 | 106411300 | 106410740 | ID=MsaT017165.1.exon1;Parent=MsaT017165.1 |
Chr3 | CDS | 106410740 | 106411300 | 106410740 | ID=cds.MsaT017165.1;Parent=MsaT017165.1 |
Gene Sequence |
Protein sequence |