AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017165


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017165 MtrunA17_Chr3g0137111 84.946 186 21 3 1 186 1 179 1.07e-106 305
MsaG017165 MtrunA17_Chr1g0149811 51.872 187 70 9 5 185 24 196 7.97e-48 156
MsaG017165 MtrunA17_Chr2g0333741 42.857 175 76 10 12 183 20 173 1.07e-26 101
MsaG017165 MtrunA17_Chr1g0161901 44.068 118 42 6 54 165 69 168 1.86e-19 82.8
MsaG017165 MtrunA17_Chr5g0434121 52.874 87 21 3 76 161 86 153 1.03e-18 80.5
MsaG017165 MtrunA17_Chr4g0001081 43.810 105 29 3 75 174 65 144 1.14e-18 80.1
MsaG017165 MtrunA17_Chr8g0376021 48.352 91 27 4 76 165 61 132 1.20e-18 80.1
MsaG017165 MtrunA17_Chr3g0110091 48.913 92 26 4 77 167 86 157 1.15e-17 77.8
MsaG017165 MtrunA17_Chr1g0207691 40.206 97 36 3 71 166 57 132 3.88e-15 70.1
MsaG017165 MtrunA17_Chr4g0072571 73.684 38 10 0 75 112 43 80 1.87e-14 67.8
MsaG017165 MtrunA17_Chr3g0081121 42.857 98 47 5 69 166 90 178 4.21e-14 68.9
MsaG017165 MtrunA17_Chr7g0250871 42.529 87 39 3 57 143 33 108 1.45e-11 60.1
MsaG017165 MtrunA17_Chr3g0079671 35.398 113 52 4 74 176 95 196 1.53e-11 61.6
MsaG017165 MtrunA17_Chr3g0081131 36.364 110 50 4 71 176 92 185 2.40e-11 61.2
MsaG017165 MtrunA17_Chr7g0222661 33.613 119 59 6 71 184 80 183 3.74e-11 60.5
MsaG017165 MtrunA17_Chr7g0254221 61.538 39 15 0 77 115 33 71 4.49e-11 58.5
MsaG017165 MtrunA17_Chr5g0396031 38.889 90 43 3 77 166 46 123 7.12e-11 60.1
MsaG017165 MtrunA17_Chr7g0251581 69.697 33 10 0 77 109 39 71 8.55e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017165 AT5G25160.1 45.385 130 46 7 48 176 37 142 5.61e-23 92.0
MsaG017165 AT1G80730.1 40.876 137 72 4 37 166 20 154 1.52e-22 90.9
MsaG017165 AT5G10970.1 37.333 150 72 5 18 166 48 176 1.01e-20 87.0
MsaG017165 AT1G66140.1 46.739 92 37 3 77 161 83 169 2.28e-16 75.1
MsaG017165 AT1G24625.1 76.316 38 9 0 74 111 54 91 1.33e-15 72.0
MsaG017165 AT5G57520.1 65.909 44 15 0 75 118 48 91 6.39e-15 68.9
MsaG017165 AT5G14010.1 73.529 34 9 0 77 110 36 69 8.83e-13 63.5
MsaG017165 AT1G67030.1 44.944 89 44 3 44 132 7 90 2.20e-11 60.5
MsaG017165 AT3G58070.1 52.459 61 24 1 56 116 73 128 4.37e-11 60.5

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGAGACCATTTGCAATTA+AGG 0.217801 3:+106411192 MsaT017165.1:CDS
GGAATTGAAAGCAATTATTA+TGG 0.251312 3:+106411265 MsaT017165.1:CDS
TCACAACCGTTATGCAAATA+TGG 0.314680 3:+106411132 MsaT017165.1:CDS
CCTAACGATGATTGTGATCT+TGG 0.347148 3:+106410842 MsaT017165.1:CDS
ATTGCAGAATCCACATGAAT+TGG 0.369569 3:+106410751 MsaT017165.1:CDS
AGCAATTATTATGGATATCA+TGG 0.375634 3:+106411274 MsaT017165.1:CDS
AGAATTATCTATGTCTAAAC+AGG 0.387114 3:-106410891 None:intergenic
TTGGATGAAAATTTATGAAA+AGG 0.404264 3:-106411235 None:intergenic
GGAGAAACATAAAACCTTTG+AGG 0.452533 3:+106410772 MsaT017165.1:CDS
AACCTTTGAGGAAGAAGAAA+AGG 0.455284 3:+106410784 MsaT017165.1:CDS
AGAACGATGTTCTCAGCAAC+AGG 0.459446 3:+106411076 MsaT017165.1:CDS
TTCTTCAGTTCACAAGCACT+TGG 0.470251 3:+106411001 MsaT017165.1:CDS
CTAACGATGATTGTGATCTT+GGG 0.483992 3:+106410843 MsaT017165.1:CDS
GCTTTCAATTCCAAAGCCAT+TGG 0.496119 3:-106411254 None:intergenic
TCTTCAGTTCACAAGCACTT+GGG 0.508835 3:+106411002 MsaT017165.1:CDS
CTTCAGTTCACAAGCACTTG+GGG 0.514360 3:+106411003 MsaT017165.1:CDS
GAGTGTGCCTTAATTGCAAA+TGG 0.520506 3:-106411199 None:intergenic
TGCAATTAAGGCACACTCGA+TGG 0.523345 3:+106411204 MsaT017165.1:CDS
CCAAGATCACAATCATCGTT+AGG 0.527605 3:-106410842 None:intergenic
GGAAGAAGAAAAGGAGGCAA+AGG 0.531006 3:+106410793 MsaT017165.1:CDS
TTATGTTTCTCCAATTCATG+TGG 0.532950 3:-106410761 None:intergenic
ATGATATTTATGAAGAAATG+AGG 0.554191 3:-106411107 None:intergenic
GAGATGCCATATTTGCATAA+CGG 0.557005 3:-106411138 None:intergenic
TCATCAGAAAACTTCACTTG+TGG 0.611490 3:+106410923 MsaT017165.1:CDS
AAAATGCTCACAAGAGAGAG+AGG 0.643637 3:+106411032 MsaT017165.1:CDS
TTCAGTTCACAAGCACTTGG+GGG 0.644584 3:+106411004 MsaT017165.1:CDS
CTTTGAGGAAGAAGAAAAGG+AGG 0.655746 3:+106410787 MsaT017165.1:CDS
ATATTTATGAAGAAATGAGG+TGG 0.662714 3:-106411104 None:intergenic
TTACTATTAGCACAGAAGAG+TGG 0.684083 3:-106411166 None:intergenic
ACGATGTTCTCAGCAACAGG+AGG 0.689396 3:+106411079 MsaT017165.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 106410740 106411300 106410740 ID=MsaG017165
Chr3 mRNA 106410740 106411300 106410740 ID=MsaT017165.1;Parent=MsaG017165
Chr3 exon 106410740 106411300 106410740 ID=MsaT017165.1.exon1;Parent=MsaT017165.1
Chr3 CDS 106410740 106411300 106410740 ID=cds.MsaT017165.1;Parent=MsaT017165.1
Gene Sequence

>MsaT017165.1

ATGGAAATATCATTGCAGAATCCACATGAATTGGAGAAACATAAAACCTTTGAGGAAGAAGAAAAGGAGGCAAAGGTAGACACTTCATTTGATCTGAATGTTCCTAACGATGATTGTGATCTTGGGTGCAGTTTAAAGTCAAATTTAGTTACCTGTTTAGACATAGATAATTCTTCTAAAACATCATCAGAAAACTTCACTTGTGGCTCTGAATCAACTTCAGAGCCACGATTTTTCACTTGCAACTACTGCAAGAGAAAATTCTTCAGTTCACAAGCACTTGGGGGACATCAAAATGCTCACAAGAGAGAGAGGTCAATTGCAAAAAGAGGACGGAGAACGATGTTCTCAGCAACAGGAGGAACCACCTCATTTCTTCATAAATATCATCTTCACAACCGTTATGCAAATATGGCATCTCTACTACCACTCTTCTGTGCTAATAGTAATAGTATGAGACCATTTGCAATTAAGGCACACTCGATGGTTCAGAAGCCTTTTCATAAATTTTCATCCAATGGCTTTGGAATTGAAAGCAATTATTATGGATATCATGGATGA

Protein sequence

>MsaT017165.1

MEISLQNPHELEKHKTFEEEEKEAKVDTSFDLNVPNDDCDLGCSLKSNLVTCLDIDNSSKTSSENFTCGSESTSEPRFFTCNYCKRKFFSSQALGGHQNAHKRERSIAKRGRRTMFSATGGTTSFLHKYHLHNRYANMASLLPLFCANSNSMRPFAIKAHSMVQKPFHKFSSNGFGIESNYYGYHG*