AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017173


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017173 MtrunA17_Chr3g0137241 96.084 383 3 2 1 383 1 371 0.0 758
MsaG017173 MtrunA17_Chr1g0149651 66.970 439 86 8 1 383 1 436 0.0 557
MsaG017173 MtrunA17_Chr2g0305101 66.006 353 79 6 31 382 42 354 7.66e-159 452
MsaG017173 MtrunA17_Chr4g0065241 80.709 254 41 3 119 370 71 318 1.68e-148 425
MsaG017173 MtrunA17_Chr5g0398181 67.033 273 78 3 119 385 28 294 1.23e-128 373
MsaG017173 MtrunA17_Chr1g0192791 35.249 261 122 8 119 367 20 245 9.16e-31 119
MsaG017173 MtrunA17_Chr2g0289201 28.102 274 166 4 119 391 118 361 1.01e-28 115
MsaG017173 MtrunA17_Chr1g0151961 32.669 251 139 7 119 367 120 342 1.41e-25 107
MsaG017173 MtrunA17_Chr5g0413201 32.669 251 138 6 119 367 73 294 2.04e-25 105
MsaG017173 MtrunA17_Chr5g0439391 29.032 279 152 7 119 391 47 285 2.45e-24 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017173 AT5G25220.2 64.989 437 81 12 1 383 1 419 9.29e-180 508
MsaG017173 AT5G25220.1 64.908 436 81 12 1 382 1 418 5.68e-179 506
MsaG017173 AT5G11060.1 67.233 412 75 12 1 382 1 382 7.46e-173 489
MsaG017173 AT4G32040.2 62.895 380 126 8 6 383 13 379 2.13e-155 444
MsaG017173 AT4G32040.1 62.895 380 126 8 6 383 13 379 2.94e-155 444
MsaG017173 AT1G62990.1 62.759 290 99 4 96 382 7 290 2.14e-126 367
MsaG017173 AT1G70510.1 33.077 260 137 8 112 367 63 289 2.04e-29 117
MsaG017173 AT1G23380.1 32.616 279 143 9 119 389 86 327 9.57e-29 115
MsaG017173 AT1G70510.2 31.923 260 144 7 112 367 63 293 6.82e-28 112
MsaG017173 AT1G23380.2 32.384 281 143 10 119 389 86 329 6.74e-27 110
MsaG017173 AT4G08150.1 33.205 259 130 8 119 367 135 360 9.79e-26 108
MsaG017173 AT1G62360.1 34.400 250 135 6 119 367 122 343 3.16e-25 106

Find 124 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAATAGTATGGGAGGATT+TGG 0.320582 3:+106513827 MsaT017173.1:CDS
TGCCAGCTGAGCGTCGATCC+TGG 0.345057 3:-106511478 None:intergenic
CGATGACTTCGCTGTGGTTT+CGG 0.353018 3:-106511278 None:intergenic
TGTACCTGTTAGACTTTGTA+AGG 0.355039 3:-106512454 None:intergenic
GTACTCTGCGTTAGGACAAC+AGG 0.363398 3:+106511524 MsaT017173.1:CDS
GGATGGTTCAGATAATAGTA+TGG 0.368115 3:+106513817 MsaT017173.1:CDS
CTTTCCAAACTGCACCTAAC+TGG 0.380352 3:+106511115 MsaT017173.1:CDS
AATTGAAACAGATCAATAAC+TGG 0.381851 3:+106514814 MsaT017173.1:CDS
GTTGTTGTTGTTCTGTGAAA+TGG 0.389827 3:-106511065 None:intergenic
TGCCTTTCAGGAAGAGGATA+AGG 0.392331 3:+106514764 MsaT017173.1:intron
CAGTCCTTACAAAGTCTAAC+AGG 0.396414 3:+106512450 MsaT017173.1:CDS
GAGCGTCGATCCTGGGCAAC+TGG 0.397329 3:-106511470 None:intergenic
TAAACAACTCGACCACTTCA+TGG 0.399427 3:+106511569 MsaT017173.1:CDS
GAGCCATTGACCGGAGGATT+GGG 0.403988 3:-106511238 None:intergenic
GCAAGGTTGGTGCAAGAAAC+CGG 0.410624 3:+106514786 MsaT017173.1:CDS
TGGTGTCGAGGTTGCGAAGA+AGG 0.418558 3:-106511149 None:intergenic
AGACGAGCTGGAAAACTTCC+TGG 0.420513 3:+106514206 MsaT017173.1:CDS
AGTGATGTTAATCTGTTTGA+TGG 0.421723 3:+106513788 MsaT017173.1:CDS
AGGGCGTTGTTGAGCCAGTT+AGG 0.423606 3:-106511129 None:intergenic
TGAGCCAGTTAGGTGCAGTT+TGG 0.424789 3:-106511119 None:intergenic
GGAAGCAGTAATGGCTTGTT+GGG 0.426655 3:+106512419 MsaT017173.1:CDS
GTTCCCAATCCTCCGGTCAA+TGG 0.427348 3:+106511235 MsaT017173.1:CDS
AATGGTGGAGTGGGTTGTCT+TGG 0.431285 3:-106511047 None:intergenic
TGGAAGCAGTAATGGCTTGT+TGG 0.435199 3:+106512418 MsaT017173.1:CDS
GAAACGCAAAGGTGAAATAA+TGG 0.435340 3:+106511380 MsaT017173.1:CDS
ATCCTGGGCAACTGGTCAAC+CGG 0.438877 3:-106511462 None:intergenic
TCAATCAAAGGAAGAGGAAC+TGG 0.439251 3:+106514841 MsaT017173.1:CDS
GAAGTTGGTACTGTTGTTGT+TGG 0.439599 3:-106511193 None:intergenic
GCCCAGGATCGACGCTCAGC+TGG 0.442737 3:+106511476 MsaT017173.1:CDS
TAATCTGTTTGATGGAGGTT+TGG 0.446200 3:+106513796 MsaT017173.1:CDS
AGAGCCATTGACCGGAGGAT+TGG 0.446472 3:-106511239 None:intergenic
GCCAGCTGAGCGTCGATCCT+GGG 0.452139 3:-106511477 None:intergenic
GGTACTGTTGTTGTTGGTTG+TGG 0.453246 3:-106511187 None:intergenic
ATTAAAGCATGAGTTGAAAC+AGG 0.455099 3:+106513904 MsaT017173.1:CDS
ATAGAAGCTGTTCATACAAC+GGG 0.456842 3:-106511407 None:intergenic
CTTCGCTGTGGTTTCGGTGG+AGG 0.457682 3:-106511272 None:intergenic
TTGTCCTAACGCAGAGTACT+TGG 0.459203 3:-106511520 None:intergenic
AACCGGCGGTGTCGAGGTTG+AGG 0.460908 3:-106511215 None:intergenic
CAGCGGAGGGAGTGATGAAC+TGG 0.464161 3:+106511349 MsaT017173.1:CDS
CGCCGGTTGACCAGTTGCCC+AGG 0.465203 3:+106511460 MsaT017173.1:CDS
CAACCTCAGTACTTAAAGAT+TGG 0.466078 3:+106514234 MsaT017173.1:CDS
TTCAACGCATTAGAAGTTGA+AGG 0.469895 3:-106514873 None:intergenic
GTTGTTGTTGGTTGTGGCGG+TGG 0.469972 3:-106511181 None:intergenic
CCATGCAATGGAAGCAGTAA+TGG 0.471444 3:+106512410 MsaT017173.1:CDS
GTTGCTCCTGTACCTTCCCC+TGG 0.471752 3:-106513734 None:intergenic
CTGTTTGATGGAGGTTTGGA+TGG 0.472583 3:+106513800 MsaT017173.1:CDS
CTGTGGTTTCGGTGGAGGAC+AGG 0.484561 3:-106511267 None:intergenic
CATGGACTCGGACATGTTGT+TGG 0.486135 3:-106512392 None:intergenic
AGAGTTCTTACAGTAGGATA+AGG 0.489180 3:-106514278 None:intergenic
CGTCACCGGCGCTGGTATGA+AGG 0.491895 3:+106511305 MsaT017173.1:CDS
GTTGCCAAGTACTCTGCGTT+AGG 0.491986 3:+106511516 MsaT017173.1:CDS
GTTTCACCAGGGGAAGGTAC+AGG 0.498577 3:+106513728 MsaT017173.1:CDS
AGTACTGAGGTTGTGTCACC+AGG 0.501554 3:-106514224 None:intergenic
CCATTACTGCTTCCATTGCA+TGG 0.504180 3:-106512410 None:intergenic
AGGACGGGAGAGCCATTGAC+CGG 0.509843 3:-106511247 None:intergenic
GTTCTGTGAAATGGTGGAGT+GGG 0.511127 3:-106511056 None:intergenic
AAGCGGTGAGGCAACAGCGG+AGG 0.511964 3:+106511335 MsaT017173.1:CDS
GCAAATTTAAGATTATGAAA+AGG 0.513629 3:-106514935 None:intergenic
GTTTCGGTGGAGGACAGGAC+GGG 0.513862 3:-106511262 None:intergenic
CTGCTTCCATTGCATGGACT+CGG 0.514678 3:-106512404 None:intergenic
TTGACCGGAGGATTGGGAAC+CGG 0.515320 3:-106511232 None:intergenic
TGTTGCCTCACCGCTTTCCT+CGG 0.515416 3:-106511328 None:intergenic
TCATTCTCTGTTAGAACAAG+AGG 0.524064 3:-106513851 None:intergenic
GGTGTCGAGGTTGCGAAGAA+GGG 0.525959 3:-106511148 None:intergenic
GAAAATTGTGGACATAAGAG+AGG 0.527462 3:+106514169 MsaT017173.1:CDS
GGTGTCGAGGTTGAGGAAGT+TGG 0.532035 3:-106511208 None:intergenic
ATTGGGAACCGGCGGTGTCG+AGG 0.533014 3:-106511221 None:intergenic
ACTTATCTCTAATTCAAGAT+CGG 0.536434 3:-106514970 None:intergenic
GATGGTTCAGATAATAGTAT+GGG 0.538925 3:+106513818 MsaT017173.1:CDS
CGTTGAAGAGCAAGCGCAAG+AGG 0.539470 3:+106514889 MsaT017173.1:CDS
CTCGGCCTTCATACCAGCGC+CGG 0.539817 3:-106511310 None:intergenic
GGTTTCGGTGGAGGACAGGA+CGG 0.540180 3:-106511263 None:intergenic
GGAAGAGGATAAGGCAAGGT+TGG 0.540633 3:+106514773 MsaT017173.1:intron
GTTGTTGTTCTGTGAAATGG+TGG 0.541053 3:-106511062 None:intergenic
GAGTGGTGAGGATCGGAGGT+TGG 0.542790 3:-106511093 None:intergenic
CCTCAGTACTTAAAGATTGG+TGG 0.548243 3:+106514237 MsaT017173.1:CDS
GAAAGAGTGGTGAGGATCGG+AGG 0.551627 3:-106511097 None:intergenic
ATCGACGACGTCACCGGCGC+TGG 0.555704 3:+106511297 MsaT017173.1:CDS
AGCGGTGAGGCAACAGCGGA+GGG 0.559972 3:+106511336 MsaT017173.1:CDS
CGGCGCTGGTATGAAGGCCG+AGG 0.563738 3:+106511311 MsaT017173.1:CDS
AGAGAATGAGAGGTCCCTCA+TGG 0.567340 3:+106513865 MsaT017173.1:CDS
ATAACTGGTTTATCAATCAA+AGG 0.567448 3:+106514829 MsaT017173.1:CDS
TGTTCTGTGAAATGGTGGAG+TGG 0.567991 3:-106511057 None:intergenic
CAAGGTTGGTGCAAGAAACC+GGG 0.570646 3:+106514787 MsaT017173.1:CDS
ACCGCCGGTTCCCAATCCTC+CGG 0.570735 3:+106511228 MsaT017173.1:CDS
GAATTAGAGATAAGTAATGC+AGG 0.571655 3:+106514978 MsaT017173.1:CDS
GAAGTCATCGACGACGTCAC+CGG 0.571705 3:+106511291 MsaT017173.1:CDS
TTCCTCAACCTCGACACCGC+CGG 0.572536 3:+106511213 MsaT017173.1:CDS
TGACTTCGCTGTGGTTTCGG+TGG 0.576108 3:-106511275 None:intergenic
GGTTTATCAATCAAAGGAAG+AGG 0.579093 3:+106514835 MsaT017173.1:CDS
TTGGAAAGAGTGGTGAGGAT+CGG 0.583594 3:-106511100 None:intergenic
CCACCAATCTTTAAGTACTG+AGG 0.588799 3:-106514237 None:intergenic
AAAGGTAAAATACCATGAAG+TGG 0.598924 3:-106511581 None:intergenic
TAGGTGCAGTTTGGAAAGAG+TGG 0.600373 3:-106511110 None:intergenic
TGGCGTTGACGTTGGTGTCG+AGG 0.601937 3:-106511161 None:intergenic
GGGTTATAAAGAGAAAATTG+TGG 0.610746 3:+106514157 MsaT017173.1:intron
TTGTTCTAACAGAGAATGAG+AGG 0.611125 3:+106513855 MsaT017173.1:CDS
TGTGGCGGTGGCGTTGACGT+TGG 0.611369 3:-106511169 None:intergenic
GGTTCAGATAATAGTATGGG+AGG 0.618510 3:+106513821 MsaT017173.1:CDS
GATGTTAATCTGTTTGATGG+AGG 0.621633 3:+106513791 MsaT017173.1:CDS
TGTGTAACAGGAGTTTCACC+AGG 0.621894 3:+106513716 MsaT017173.1:intron
TGGCAGAAAGCGAAACGCAA+AGG 0.622833 3:+106511369 MsaT017173.1:CDS
GATCTGTTTCAATTGCAACC+CGG 0.623157 3:-106514805 None:intergenic
ACCGGAGGATTGGGAACCGG+CGG 0.625286 3:-106511229 None:intergenic
GGAAAGCGGTGAGGCAACAG+CGG 0.626255 3:+106511332 MsaT017173.1:CDS
GGGCAACTGGTCAACCGGCG+TGG 0.628223 3:-106511457 None:intergenic
ATGTCTTACACGTTCCATGA+GGG 0.635061 3:-106513879 None:intergenic
ACAGGAGTTTCACCAGGGGA+AGG 0.636992 3:+106513722 MsaT017173.1:intron
ACATGTCCGAGTCCATGCAA+TGG 0.642083 3:+106512398 MsaT017173.1:CDS
ACTGTTGTTGTTGGTTGTGG+CGG 0.642777 3:-106511184 None:intergenic
CATGTCTTACACGTTCCATG+AGG 0.645094 3:-106513880 None:intergenic
GATAGAAGCTGTTCATACAA+CGG 0.647941 3:-106511408 None:intergenic
TACTCTGCGTTAGGACAACA+GGG 0.650241 3:+106511525 MsaT017173.1:CDS
GGTATGAAGGCCGAGGAAAG+CGG 0.652275 3:+106511318 MsaT017173.1:CDS
ACGGGAGAGCCATTGACCGG+AGG 0.652694 3:-106511244 None:intergenic
GTGTTTGAGAATCGCCACGC+CGG 0.654940 3:+106511443 MsaT017173.1:CDS
TTCAGGAAGAGGATAAGGCA+AGG 0.655439 3:+106514769 MsaT017173.1:intron
AATGCAGGTGATAAAAGTGA+CGG 0.675503 3:+106514993 MsaT017173.1:CDS
GGTGGCAATCACATTCAAAG+TGG 0.680210 3:+106514255 MsaT017173.1:CDS
GCAGTTTGGAAAGAGTGGTG+AGG 0.695329 3:-106511105 None:intergenic
CGTCGTCGATGACTTCGCTG+TGG 0.697602 3:-106511284 None:intergenic
GTGTAACAGGAGTTTCACCA+GGG 0.706920 3:+106513717 MsaT017173.1:intron
GAAGGCCGAGGAAAGCGGTG+AGG 0.708487 3:+106511323 MsaT017173.1:CDS
TGTAACAGGAGTTTCACCAG+GGG 0.775474 3:+106513718 MsaT017173.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 106511033 106515028 106511033 ID=MsaG017173
Chr3 mRNA 106511033 106515028 106511033 ID=MsaT017173.1;Parent=MsaG017173
Chr3 exon 106511033 106511590 106511033 ID=MsaT017173.1.exon1;Parent=MsaT017173.1
Chr3 CDS 106511033 106511590 106511033 ID=cds.MsaT017173.1;Parent=MsaT017173.1
Chr3 exon 106512351 106512471 106512351 ID=MsaT017173.1.exon2;Parent=MsaT017173.1
Chr3 CDS 106512351 106512471 106512351 ID=cds.MsaT017173.1;Parent=MsaT017173.1
Chr3 exon 106513726 106513925 106513726 ID=MsaT017173.1.exon3;Parent=MsaT017173.1
Chr3 CDS 106513726 106513925 106513726 ID=cds.MsaT017173.1;Parent=MsaT017173.1
Chr3 exon 106514158 106514289 106514158 ID=MsaT017173.1.exon4;Parent=MsaT017173.1
Chr3 CDS 106514158 106514289 106514158 ID=cds.MsaT017173.1;Parent=MsaT017173.1
Chr3 exon 106514774 106515028 106514774 ID=MsaT017173.1.exon5;Parent=MsaT017173.1
Chr3 CDS 106514774 106515028 106514774 ID=cds.MsaT017173.1;Parent=MsaT017173.1
Gene Sequence

>MsaT017173.1

ATGGCGTATCACAACCAAGACAACCCACTCCACCATTTCACAGAACAACAACAACAACAACCAACCTCCGATCCTCACCACTCTTTCCAAACTGCACCTAACTGGCTCAACAACGCCCTTCTTCGCAACCTCGACACCAACGTCAACGCCACCGCCACAACCAACAACAACAGTACCAACTTCCTCAACCTCGACACCGCCGGTTCCCAATCCTCCGGTCAATGGCTCTCCCGTCCTGTCCTCCACCGAAACCACAGCGAAGTCATCGACGACGTCACCGGCGCTGGTATGAAGGCCGAGGAAAGCGGTGAGGCAACAGCGGAGGGAGTGATGAACTGGCAGAAAGCGAAACGCAAAGGTGAAATAATGGCGCACCCGTTGTATGAACAGCTTCTATCAGCACACGTGTCGTGTTTGAGAATCGCCACGCCGGTTGACCAGTTGCCCAGGATCGACGCTCAGCTGGCAGAGTCACAGAATGTTGTTGCCAAGTACTCTGCGTTAGGACAACAGGGCATGCTTGATAACGATGATAATAAACAACTCGACCACTTCATGTCGCATTATGTTTTGTTGCTTTGTTCGTTCAAAGAACAGCTCCAACAACATGTCCGAGTCCATGCAATGGAAGCAGTAATGGCTTGTTGGGAGATCGAACAGTCCTTACAAAGTCTAACAGGAGTTTCACCAGGGGAAGGTACAGGAGCAACAATGTCTGAAGATGAAGATGAGCAAGTAGACAGTGATGTTAATCTGTTTGATGGAGGTTTGGATGGTTCAGATAATAGTATGGGAGGATTTGGTCCTCTTGTTCTAACAGAGAATGAGAGGTCCCTCATGGAACGTGTAAGACATGAATTAAAGCATGAGTTGAAACAGGGTTATAAAGAGAAAATTGTGGACATAAGAGAGGAAATTTTACGCAAGAGACGAGCTGGAAAACTTCCTGGTGACACAACCTCAGTACTTAAAGATTGGTGGCAATCACATTCAAAGTGGCCTTATCCTACTGAAGAGGATAAGGCAAGGTTGGTGCAAGAAACCGGGTTGCAATTGAAACAGATCAATAACTGGTTTATCAATCAAAGGAAGAGGAACTGGCACAGCAATCCTTCAACTTCTAATGCGTTGAAGAGCAAGCGCAAGAGGTATTTCTATATTAAAAAAATAGTCCTTTTCATAATCTTAAATTTGCATGAACTCTGCACCGATCTTGAATTAGAGATAAGTAATGCAGGTGATAAAAGTGACGGTCGTTACATGTAA

Protein sequence

>MsaT017173.1

MAYHNQDNPLHHFTEQQQQQPTSDPHHSFQTAPNWLNNALLRNLDTNVNATATTNNNSTNFLNLDTAGSQSSGQWLSRPVLHRNHSEVIDDVTGAGMKAEESGEATAEGVMNWQKAKRKGEIMAHPLYEQLLSAHVSCLRIATPVDQLPRIDAQLAESQNVVAKYSALGQQGMLDNDDNKQLDHFMSHYVLLLCSFKEQLQQHVRVHAMEAVMACWEIEQSLQSLTGVSPGEGTGATMSEDEDEQVDSDVNLFDGGLDGSDNSMGGFGPLVLTENERSLMERVRHELKHELKQGYKEKIVDIREEILRKRRAGKLPGDTTSVLKDWWQSHSKWPYPTEEDKARLVQETGLQLKQINNWFINQRKRNWHSNPSTSNALKSKRKRYFYIKKIVLFIILNLHELCTDLELEISNAGDKSDGRYM*