AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017228


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017228 MtrunA17_Chr3g0137971 92.470 332 17 2 1 326 1 330 0.0 567
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017228 AT5G11270.1 48.659 261 127 1 66 319 91 351 7.51e-68 216

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCTCCGGGCGTAGTTTC+AGG 0.263805 3:+107072660 None:intergenic
GGTGATACTGCTGATGGTTT+AGG 0.275225 3:+107072681 None:intergenic
TTCTCGAAAGAGATTGAAAA+AGG 0.338696 3:-107072544 MsaT017228.1:CDS
GATCATGCTCATGCAGAATC+TGG 0.343580 3:-107072615 MsaT017228.1:CDS
TTCCTCTGACTGTGACTAAA+CGG 0.395790 3:+107074227 None:intergenic
TTCGCTGCAAAGAAGAGGAT+TGG 0.420945 3:+107074203 None:intergenic
GCTGATGGTTTAGGTTCATC+AGG 0.425177 3:+107072690 None:intergenic
TTCATGACATGCAACAGAAT+TGG 0.427701 3:-107072569 MsaT017228.1:CDS
CGCGGAGAACAGTTATTAAG+TGG 0.452000 3:-107071215 MsaT017228.1:CDS
TCGCTGCAAAGAAGAGGATT+GGG 0.456309 3:+107074204 None:intergenic
TTCTGTTGCATGTCATGAAT+AGG 0.457484 3:+107072573 None:intergenic
TTGGCTAGAGCATTGAAAAC+TGG 0.460688 3:-107073650 MsaT017228.1:CDS
ATAGGTAAGTTAGACTTCTT+CGG 0.466152 3:+107072591 None:intergenic
TCCTCTGACTGTGACTAAAC+GGG 0.466238 3:+107074228 None:intergenic
CGTGAGTTGGAAGGTGTGTT+GGG 0.479759 3:-107073797 MsaT017228.1:CDS
TGTTTGAAAAGCAACTCAAA+TGG 0.492775 3:+107073920 None:intergenic
ATTCGCTGAAGCTTAGAACT+TGG 0.492933 3:-107073685 MsaT017228.1:CDS
TCGTTAGCAGACTGCTGGTA+AGG 0.494872 3:+107071147 None:intergenic
TTGTTTCGTTAGCAGACTGC+TGG 0.504326 3:+107071142 None:intergenic
AAAGGCTTACACTTAGTTTG+CGG 0.505407 3:+107073624 None:intergenic
TGCTTTGTTAGAACGTGAGT+TGG 0.513640 3:-107073810 MsaT017228.1:CDS
CTCAGCATCAGAAGACTTGG+TGG 0.517452 3:+107072723 None:intergenic
AGCTCTATCAAGACAGAGCT+CGG 0.525713 3:+107072773 None:intergenic
AGAGCGAGAAGGAAGAGTGA+AGG 0.528288 3:+107074165 None:intergenic
ACGTGAGTTGGAAGGTGTGT+TGG 0.531369 3:-107073798 MsaT017228.1:CDS
AGAGTGAAGGAGAAGAGAAG+AGG 0.540225 3:+107074178 None:intergenic
AGACAAACGTGCCGAGGAGG+GGG 0.542683 3:-107071187 MsaT017228.1:CDS
GTTTCAGGTGATACTGCTGA+TGG 0.555775 3:+107072675 None:intergenic
AGGCTCAGCATCAGAAGACT+TGG 0.557597 3:+107072720 None:intergenic
CGGTGACAATTGAAGAGTAG+AGG 0.560168 3:+107074137 None:intergenic
TGGTAAGGAAGACGATGCTC+TGG 0.560826 3:+107071162 None:intergenic
AAATTGACTCATGTTACAGA+GGG 0.567201 3:-107073959 MsaT017228.1:CDS
AACTTGGCAATTGAATACAT+TGG 0.575518 3:-107073669 MsaT017228.1:CDS
AGCATCAGAAGACTTGGTGG+AGG 0.576518 3:+107072726 None:intergenic
TAGGTTCATCAGGAATCGAT+AGG 0.584575 3:+107072700 None:intergenic
GTATCACCTGAAACTACGCC+CGG 0.589871 3:-107072666 MsaT017228.1:CDS
GAAGACAAACGTGCCGAGGA+GGG 0.595141 3:-107071189 MsaT017228.1:CDS
AAAGGCTCAACTTGACACGC+TGG 0.611700 3:-107072526 MsaT017228.1:CDS
TTGTTAGAACGTGAGTTGGA+AGG 0.619119 3:-107073806 MsaT017228.1:CDS
TGAAGACAAACGTGCCGAGG+AGG 0.619168 3:-107071190 MsaT017228.1:CDS
AAGACAAACGTGCCGAGGAG+GGG 0.620622 3:-107071188 MsaT017228.1:CDS
TGCATGTTTCAAACATTCCG+CGG 0.630435 3:-107071233 MsaT017228.1:CDS
ACCCGTTTAGTCACAGTCAG+AGG 0.634786 3:-107074229 MsaT017228.1:CDS
AAAATTGACTCATGTTACAG+AGG 0.638213 3:-107073960 MsaT017228.1:CDS
GCATCAGAAGACTTGGTGGA+GGG 0.649310 3:+107072727 None:intergenic
GTTTGAAGACAAACGTGCCG+AGG 0.651860 3:-107071193 MsaT017228.1:CDS
TGATAAACACTGATTCGCGA+CGG 0.673675 3:+107074255 None:intergenic
TGTTACAGAGGGGAATGACG+AGG 0.686418 3:-107073948 MsaT017228.1:CDS
TACAGAGGGGAATGACGAGG+AGG 0.700089 3:-107073945 MsaT017228.1:CDS
ACAGATATTAGCGAAACACA+AGG 0.710555 3:-107073749 MsaT017228.1:CDS
CACTTAATAACTGTTCTCCG+CGG 0.713242 3:+107071216 None:intergenic
GAGTTTACATGAGATCAAAG+CGG 0.731573 3:-107072500 MsaT017228.1:CDS
AATTGACTCATGTTACAGAG+GGG 0.739510 3:-107073958 MsaT017228.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 107071137 107074281 107071137 ID=MsaG017228
Chr3 mRNA 107071137 107074281 107071137 ID=MsaT017228.1;Parent=MsaG017228
Chr3 exon 107071137 107071277 107071137 ID=MsaT017228.1.exon4;Parent=MsaT017228.1
Chr3 CDS 107071137 107071277 107071137 ID=cds.MsaT017228.1;Parent=MsaT017228.1
Chr3 exon 107072494 107072811 107072494 ID=MsaT017228.1.exon3;Parent=MsaT017228.1
Chr3 CDS 107072494 107072811 107072494 ID=cds.MsaT017228.1;Parent=MsaT017228.1
Chr3 exon 107073634 107073984 107073634 ID=MsaT017228.1.exon2;Parent=MsaT017228.1
Chr3 CDS 107073634 107073984 107073634 ID=cds.MsaT017228.1;Parent=MsaT017228.1
Chr3 exon 107074111 107074281 107074111 ID=MsaT017228.1.exon1;Parent=MsaT017228.1
Chr3 CDS 107074111 107074281 107074111 ID=cds.MsaT017228.1;Parent=MsaT017228.1
Gene Sequence

>MsaT017228.1

ATGGCCGTCGCGAATCAGTGTTTATCATATACCCGTTTAGTCACAGTCAGAGGAACCCAATCCTCTTCTTTGCAGCGAAAACCTCTTCTCTTCTCCTTCACTCTTCCTTCTCGCTCTCAAAACCTCTACTCTTCAATTGTCACCGCCTCTTCCAAAAAGAAAAACAATAAGAAAAAATTGACTCATGTTACAGAGGGGAATGACGAGGAGGATCCATTTGAGTTGCTTTTCAAACAACTCGAAGAAGATCTCAAAAACGATGATGATTTGTCAAAAGAAGATAGCGATGACGACGACGACGACGAGATAAGTGACGAAGATTTTGCTTTGTTAGAACGTGAGTTGGAAGGTGTGTTGGGTGAATTTGATGCTGAATTGTTGAACACAGATATTAGCGAAACACAAGGTGATAATGATGTAGAAAAAAGTAGTGGCGATGGAAATGATAATTCGCTGAAGCTTAGAACTTGGCAATTGAATACATTGGCTAGAGCATTGAAAACTGGACGCCGCAAACTAAGTATAAAAGCTCTTGCTGCCGAGCTCTGTCTTGATAGAGCTCTTGTTCTTGATTTGCTTCGTAACCCTCCACCAAGTCTTCTGATGCTGAGCCTATCGATTCCTGATGAACCTAAACCATCAGCAGTATCACCTGAAACTACGCCCGGAGATTCTTTTTATAAAGAAACAAGTGCAGATCATGCTCATGCAGAATCTGGACCGAAGAAGTCTAACTTACCTATTCATGACATGCAACAGAATTGGTCTTCTCGAAAGAGATTGAAAAAGGCTCAACTTGACACGCTGGAGAGAGTTTACATGAGATCAAAGCGGCCCACTAATGCAATGATTAGTAATATTGTGCATGTTTCAAACATTCCGCGGAGAACAGTTATTAAGTGGTTTGAAGACAAACGTGCCGAGGAGGGGGTTCCAGAGCATCGTCTTCCTTACCAGCAGTCTGCTAACGAAACAAGTTGA

Protein sequence

>MsaT017228.1

MAVANQCLSYTRLVTVRGTQSSSLQRKPLLFSFTLPSRSQNLYSSIVTASSKKKNNKKKLTHVTEGNDEEDPFELLFKQLEEDLKNDDDLSKEDSDDDDDDEISDEDFALLERELEGVLGEFDAELLNTDISETQGDNDVEKSSGDGNDNSLKLRTWQLNTLARALKTGRRKLSIKALAAELCLDRALVLDLLRNPPPSLLMLSLSIPDEPKPSAVSPETTPGDSFYKETSADHAHAESGPKKSNLPIHDMQQNWSSRKRLKKAQLDTLERVYMRSKRPTNAMISNIVHVSNIPRRTVIKWFEDKRAEEGVPEHRLPYQQSANETS*