AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017353


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017353 MtrunA17_Chr3g0139301 88.806 268 21 4 1 267 1 260 1.35e-170 473
MsaG017353 MtrunA17_Chr1g0147491 58.608 273 87 10 21 272 51 318 1.07e-79 244
MsaG017353 MtrunA17_Chr3g0139571 82.432 74 13 0 127 200 1 74 8.14e-31 110
MsaG017353 MtrunA17_Chr1g0190761 49.306 144 66 4 98 238 177 316 1.60e-23 99.0
MsaG017353 MtrunA17_Chr7g0274141 50.725 138 61 3 78 215 150 280 1.48e-22 95.9
MsaG017353 MtrunA17_Chr1g0155551 32.203 118 80 0 100 217 2 119 3.63e-13 65.9
MsaG017353 MtrunA17_Chr4g0035414 35.484 93 60 0 115 207 17 109 3.16e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017353 AT5G24800.1 49.730 185 81 5 73 251 62 240 1.49e-41 144
MsaG017353 AT5G28770.2 48.905 137 64 3 102 238 129 259 7.71e-21 90.5
MsaG017353 AT5G28770.1 48.905 137 64 3 102 238 122 252 7.83e-21 90.5
MsaG017353 AT5G28770.3 53.000 100 45 1 102 201 129 226 1.10e-16 78.2
MsaG017353 AT4G02640.3 47.917 96 47 1 126 221 221 313 1.22e-13 70.9
MsaG017353 AT4G02640.2 47.917 96 47 1 126 221 221 313 1.25e-13 70.9
MsaG017353 AT4G02640.4 47.917 96 47 1 126 221 215 307 1.34e-13 70.9
MsaG017353 AT4G02640.1 47.917 96 47 1 126 221 215 307 1.35e-13 70.9
MsaG017353 AT3G54620.1 46.429 84 45 0 126 209 229 312 2.69e-11 63.9

Find 63 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTCAGCCATCTATTTA+TGG 0.124761 3:+108318642 MsaT017353.1:CDS
TTTGGTTTATTAGCTGAAAC+TGG 0.195300 3:-108318775 None:intergenic
TTTGACAGAAATACCTTCTA+TGG 0.244115 3:-108318909 None:intergenic
GCGTGCTGGTCGAATCTTGC+TGG 0.252896 3:+108319664 MsaT017353.1:CDS
ATATTATTCTCTTTGTTGTT+TGG 0.273240 3:-108318793 None:intergenic
AGTTTACCGGTAGGAGAGTT+TGG 0.295352 3:-108315529 None:intergenic
TCTTGCTGGGTTTGGAAACT+TGG 0.304648 3:+108319678 MsaT017353.1:CDS
AACACTATACAAACAGTTTA+CGG 0.327856 3:+108319278 MsaT017353.1:CDS
AGCACTGATGGTGGAATGTT+TGG 0.379902 3:-108318613 None:intergenic
TGGTCGAATCTTGCTGGGTT+TGG 0.387926 3:+108319670 MsaT017353.1:CDS
AATGTTTGGGAGTGAAGTTT+TGG 0.394976 3:-108318599 None:intergenic
ATTTCCCTGCAGGACATTGA+TGG 0.400011 3:-108319610 None:intergenic
ATGTGGCATGCGGCGAATAC+TGG 0.408973 3:-108319577 None:intergenic
TGTCGATTTACATCATTGTA+AGG 0.409759 3:-108319706 None:intergenic
TGAATCTCGATTACGAGTTT+CGG 0.410788 3:+108315450 MsaT017353.1:CDS
TTGATATGAAACGCCATAGA+AGG 0.425277 3:+108318896 MsaT017353.1:CDS
GGTGAAGTTAGCTGAGGATA+TGG 0.427027 3:+108319464 MsaT017353.1:intron
TTTGTCTGAACTTGAGTCTC+AGG 0.431765 3:+108319065 MsaT017353.1:CDS
CGTGCTGGTCGAATCTTGCT+GGG 0.432249 3:+108319665 MsaT017353.1:CDS
AACCGCAACACCACCGTATG+AGG 0.448822 3:-108319640 None:intergenic
TCCGACGAGGATAATGAATC+AGG 0.453955 3:+108318847 MsaT017353.1:CDS
GTATCCAAGTCTTGCCGATA+CGG 0.463283 3:+108315472 MsaT017353.1:CDS
CAGCCATCTATTTATGGCAC+TGG 0.467277 3:+108318648 MsaT017353.1:CDS
CTTGCTGGGTTTGGAAACTT+GGG 0.468603 3:+108319679 MsaT017353.1:CDS
GCCAACCATCAATGTCCTGC+AGG 0.476677 3:+108319605 MsaT017353.1:CDS
ATACCAGTGCCATAAATAGA+TGG 0.477311 3:-108318651 None:intergenic
TGGTGTTGCGGTTGGCGTGC+TGG 0.478909 3:+108319650 MsaT017353.1:CDS
ACAAAGAGAATAATATCAAA+GGG 0.493368 3:+108318800 MsaT017353.1:CDS
CCCTGCAGGACATTGATGGT+TGG 0.501779 3:-108319606 None:intergenic
CGCTTTCCAAACTCTCCTAC+CGG 0.524112 3:+108315523 MsaT017353.1:CDS
GCCTCCGTATCGGCAAGACT+TGG 0.526307 3:-108315476 None:intergenic
TTGGTTTATTAGCTGAAACT+GGG 0.535446 3:-108318774 None:intergenic
AGGCACTATTATTTCCCTGC+AGG 0.536074 3:-108319620 None:intergenic
GCACTGATGGTGGAATGTTT+GGG 0.539183 3:-108318612 None:intergenic
TGTAGAAGCTTTGAGAGCTA+AGG 0.540256 3:+108319356 MsaT017353.1:CDS
AACAAAGAGAATAATATCAA+AGG 0.546304 3:+108318799 MsaT017353.1:CDS
GGAAATAATAGTGCCTCATA+CGG 0.548614 3:+108319627 MsaT017353.1:CDS
GATACGGAGGCATTCATGAC+CGG 0.550151 3:+108315488 MsaT017353.1:CDS
ACCTGATTCATTATCCTCGT+CGG 0.562154 3:-108318848 None:intergenic
AGAATCAATAGTAGCACTGA+TGG 0.570342 3:-108318625 None:intergenic
GCGAGTCAGCCAAGAGGTCA+AGG 0.571020 3:+108319025 MsaT017353.1:CDS
AAACTGATTGTTTAGTGATG+TGG 0.571542 3:-108319505 None:intergenic
CCGGCGAGCGATGGCAACAA+CGG 0.577221 3:+108315406 None:intergenic
ATCAATAGTAGCACTGATGG+TGG 0.581341 3:-108318622 None:intergenic
GATTCATTATCCTCGTCGGA+TGG 0.582930 3:-108318844 None:intergenic
GAAAGCGGCGGAGCAAACTC+CGG 0.584176 3:-108315507 None:intergenic
GGTCATGAATGCCTCCGTAT+CGG 0.589824 3:-108315486 None:intergenic
TCATACGGTGGTGTTGCGGT+TGG 0.590129 3:+108319642 MsaT017353.1:CDS
AATATCAAAGGGACATCAAG+TGG 0.598499 3:+108318811 MsaT017353.1:CDS
TACTATTCAACAATTTCATG+AGG 0.600013 3:+108319302 MsaT017353.1:CDS
TGCCTCATACGGTGGTGTTG+CGG 0.602335 3:+108319638 MsaT017353.1:CDS
AATAATAGTGCCTCATACGG+TGG 0.620063 3:+108319630 MsaT017353.1:CDS
TCCAAGTCTTGCCGATACGG+AGG 0.642233 3:+108315475 MsaT017353.1:CDS
CCAACCATCAATGTCCTGCA+GGG 0.644784 3:+108319606 MsaT017353.1:CDS
TAGGAGAGTTTGGAAAGCGG+CGG 0.671217 3:-108315519 None:intergenic
ATAATAGGTGAAGTTAGCTG+AGG 0.671237 3:+108319458 MsaT017353.1:intron
AGTCAGCCAAGAGGTCAAGG+TGG 0.684615 3:+108319028 MsaT017353.1:CDS
CGGTAGGAGAGTTTGGAAAG+CGG 0.687279 3:-108315522 None:intergenic
CTGGTAGTCATGTTGAGTTG+TGG 0.702154 3:-108319558 None:intergenic
TTCATCTGATCCATCCGACG+AGG 0.710432 3:+108318834 MsaT017353.1:CDS
CTAATTGCGAGTCAGCCAAG+AGG 0.711883 3:+108319019 MsaT017353.1:CDS
TTGGCGAAACATGTGGCATG+CGG 0.727060 3:-108319587 None:intergenic
TTGATGGTTGGCGAAACATG+TGG 0.736785 3:-108319594 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 108315416 108319716 108315416 ID=MsaG017353
Chr3 mRNA 108315416 108319716 108315416 ID=MsaT017353.1;Parent=MsaG017353
Chr3 exon 108315416 108315544 108315416 ID=MsaT017353.1.exon1;Parent=MsaT017353.1
Chr3 CDS 108315416 108315544 108315416 ID=cds.MsaT017353.1;Parent=MsaT017353.1
Chr3 exon 108318555 108318669 108318555 ID=MsaT017353.1.exon2;Parent=MsaT017353.1
Chr3 CDS 108318555 108318669 108318555 ID=cds.MsaT017353.1;Parent=MsaT017353.1
Chr3 exon 108318767 108318917 108318767 ID=MsaT017353.1.exon3;Parent=MsaT017353.1
Chr3 CDS 108318767 108318917 108318767 ID=cds.MsaT017353.1;Parent=MsaT017353.1
Chr3 exon 108319011 108319086 108319011 ID=MsaT017353.1.exon4;Parent=MsaT017353.1
Chr3 CDS 108319011 108319086 108319011 ID=cds.MsaT017353.1;Parent=MsaT017353.1
Chr3 exon 108319252 108319377 108319252 ID=MsaT017353.1.exon5;Parent=MsaT017353.1
Chr3 CDS 108319252 108319377 108319252 ID=cds.MsaT017353.1;Parent=MsaT017353.1
Chr3 exon 108319465 108319716 108319465 ID=MsaT017353.1.exon6;Parent=MsaT017353.1
Chr3 CDS 108319465 108319716 108319465 ID=cds.MsaT017353.1;Parent=MsaT017353.1
Gene Sequence

>MsaT017353.1

ATGGCAACAACGGAATCTGATCTTGATGAGTTTTTGAATCTCGATTACGAGTTTCGGTATCCAAGTCTTGCCGATACGGAGGCATTCATGACCGGAGTTTGCTCCGCCGCTTTCCAAACTCTCCTACCGGATGTTGTGAGTTCATTTTCAACTTGTGGTGGACTAACAGACTCCCAAAACTTCACTCCCAAACATTCCACCATCAGTGCTACTATTGATTCTCAGCCATCTATTTATGGCACTGTTGGAAGCCCAGTTTCAGCTAATAAACCAAACAACAAAGAGAATAATATCAAAGGGACATCAAGTGGTTCATCTGATCCATCCGACGAGGATAATGAATCAGGTCATTGTGAACAAATTACAAATAAACTTGATATGAAACGCCATAGAAGAAGGGTTTCTAATTGCGAGTCAGCCAAGAGGTCAAGGTGGAGAAAACAAGCACATTTGTCTGAACTTGAGTCTCAGGTTGAAAAACTGCAACTGGAAAACGCAACACTATACAAACAGTTTACGGATACTATTCAACAATTTCATGAGGCTGATACAAATAACCGAGTGCTAAAATCAGATGTAGAAGCTTTGAGAGCTAAGGTGAAGTTAGCTGAGGATATGGTCACTAGAAGTTCTTTCACCACATCACTAAACAATCAGTTTCTTCATAATCAACGTCAAATGAGCACAACACCACAACTCAACATGACTACCAGTATTCGCCGCATGCCACATGTTTCGCCAACCATCAATGTCCTGCAGGGAAATAATAGTGCCTCATACGGTGGTGTTGCGGTTGGCGTGCTGGTCGAATCTTGCTGGGTTTGGAAACTTGGGCGTGCCTTACAATGA

Protein sequence

>MsaT017353.1

MATTESDLDEFLNLDYEFRYPSLADTEAFMTGVCSAAFQTLLPDVVSSFSTCGGLTDSQNFTPKHSTISATIDSQPSIYGTVGSPVSANKPNNKENNIKGTSSGSSDPSDEDNESGHCEQITNKLDMKRHRRRVSNCESAKRSRWRKQAHLSELESQVEKLQLENATLYKQFTDTIQQFHEADTNNRVLKSDVEALRAKVKLAEDMVTRSSFTTSLNNQFLHNQRQMSTTPQLNMTTSIRRMPHVSPTINVLQGNNSASYGGVAVGVLVESCWVWKLGRALQ*