Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017433 | XP_003613438.2 | 97.436 | 39 | 1 | 0 | 42 | 80 | 46 | 84 | 1.73e-15 | 80.9 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017433 | sp|O04539|SNL4_ARATH | 81.579 | 38 | 7 | 0 | 42 | 79 | 17 | 54 | 2.17e-13 | 66.6 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017433 | tr|G7K6W4|G7K6W4_MEDTR | 97.436 | 39 | 1 | 0 | 42 | 80 | 46 | 84 | 8.25e-16 | 80.9 |
Gene ID | Type | Classification |
---|---|---|
MsaG017433 | TR | Others |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG017433 | MsaG017434 | 0.825075 | 1.013151e-53 | 2.553889e-51 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017433 | MtrunA17_Chr5g0415031 | 97.436 | 39 | 1 | 0 | 42 | 80 | 46 | 84 | 1.59e-19 | 80.9 |
MsaG017433 | MtrunA17_Chr8g0364231 | 71.795 | 39 | 11 | 0 | 42 | 80 | 45 | 83 | 5.80e-14 | 65.1 |
MsaG017433 | MtrunA17_Chr1g0206771 | 56.364 | 55 | 21 | 1 | 28 | 79 | 2 | 56 | 2.82e-12 | 58.9 |
MsaG017433 | MtrunA17_Chr6g0452001 | 71.053 | 38 | 11 | 0 | 42 | 79 | 66 | 103 | 4.20e-12 | 59.7 |
MsaG017433 | MtrunA17_Chr1g0206781 | 61.538 | 39 | 15 | 0 | 42 | 80 | 14 | 52 | 1.39e-11 | 55.8 |
MsaG017433 | MtrunA17_Chr1g0205811 | 64.103 | 39 | 14 | 0 | 42 | 80 | 17 | 55 | 4.30e-11 | 54.3 |
MsaG017433 | MtrunA17_Chr6g0452021 | 65.789 | 38 | 13 | 0 | 42 | 79 | 46 | 83 | 5.67e-11 | 56.6 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017433 | AT1G59890.2 | 83.784 | 37 | 6 | 0 | 43 | 79 | 49 | 85 | 2.07e-14 | 66.6 |
MsaG017433 | AT1G70060.2 | 81.579 | 38 | 7 | 0 | 42 | 79 | 17 | 54 | 2.20e-14 | 66.6 |
MsaG017433 | AT1G70060.1 | 81.579 | 38 | 7 | 0 | 42 | 79 | 17 | 54 | 2.20e-14 | 66.6 |
MsaG017433 | AT1G59890.1 | 83.784 | 37 | 6 | 0 | 43 | 79 | 49 | 85 | 2.49e-14 | 66.6 |
MsaG017433 | AT1G59890.3 | 83.784 | 37 | 6 | 0 | 43 | 79 | 49 | 85 | 2.62e-14 | 66.2 |
MsaG017433 | AT1G59890.4 | 83.784 | 37 | 6 | 0 | 43 | 79 | 49 | 85 | 3.37e-14 | 66.2 |
MsaG017433 | AT1G10450.3 | 52.174 | 69 | 30 | 2 | 15 | 80 | 57 | 125 | 4.35e-14 | 65.9 |
MsaG017433 | AT1G10450.2 | 52.174 | 69 | 30 | 2 | 15 | 80 | 6 | 74 | 4.98e-14 | 65.5 |
MsaG017433 | AT1G10450.1 | 52.174 | 69 | 30 | 2 | 15 | 80 | 6 | 74 | 4.98e-14 | 65.5 |
MsaG017433 | AT1G24190.3 | 73.684 | 38 | 10 | 0 | 42 | 79 | 17 | 54 | 2.12e-12 | 60.8 |
MsaG017433 | AT1G24190.1 | 73.684 | 38 | 10 | 0 | 42 | 79 | 17 | 54 | 2.25e-12 | 60.8 |
MsaG017433 | AT1G24190.2 | 73.684 | 38 | 10 | 0 | 42 | 79 | 17 | 54 | 2.41e-12 | 60.8 |
MsaG017433 | AT3G01320.2 | 68.421 | 38 | 12 | 0 | 42 | 79 | 60 | 97 | 4.33e-12 | 60.1 |
MsaG017433 | AT3G01320.1 | 68.421 | 38 | 12 | 0 | 42 | 79 | 60 | 97 | 4.37e-12 | 60.1 |
MsaG017433 | AT5G15020.2 | 53.061 | 49 | 23 | 0 | 31 | 79 | 44 | 92 | 3.05e-11 | 57.8 |
MsaG017433 | AT5G15020.1 | 53.061 | 49 | 23 | 0 | 31 | 79 | 44 | 92 | 3.05e-11 | 57.8 |
MsaG017433 | AT5G15025.1 | 68.421 | 38 | 12 | 0 | 42 | 79 | 50 | 87 | 3.36e-11 | 55.1 |
Find 10 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
GAATGTGCCTTCTTATTTGA+AGG | 0.371628 | 3:-109226843 | MsaT017433.1:intron |
TTAATCAAACCTGAGATTGT+TGG | 0.433476 | 3:+109226243 | None:intergenic |
GCTGATGGAGCTGTGGACTT+TGG | 0.439632 | 3:-109226871 | None:intergenic |
ATTAACGTGCCAACAATCTC+AGG | 0.477289 | 3:-109226252 | MsaT017433.1:intron |
AACAATACCTTCAAATAAGA+AGG | 0.490879 | 3:+109226836 | None:intergenic |
AGACAAGAAGGAAAAGTATG+AGG | 0.539440 | 3:-109226019 | MsaT017433.1:CDS |
AGCATATCTCAAGGCGATCA+AGG | 0.551355 | 3:-109226052 | MsaT017433.1:CDS |
TTCAGGGATTGATTCTAATG+TGG | 0.588863 | 3:-109226313 | MsaT017433.1:intron |
TGATGTTGTAGCATATCTCA+AGG | 0.606029 | 3:-109226061 | MsaT017433.1:intron |
ATTTGTAGAGTTGATACTGA+TGG | 0.633224 | 3:-109225870 | MsaT017433.1:intron |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr3 | gene | 109225857 | 109226889 | 109225857 | ID=MsaG017433 |
Chr3 | mRNA | 109225857 | 109226889 | 109225857 | ID=MsaT017433.1;Parent=MsaG017433 |
Chr3 | exon | 109225857 | 109225884 | 109225857 | ID=MsaT017433.1.exon4;Parent=MsaT017433.1 |
Chr3 | CDS | 109225857 | 109225884 | 109225857 | ID=cds.MsaT017433.1;Parent=MsaT017433.1 |
Chr3 | exon | 109225982 | 109226072 | 109225982 | ID=MsaT017433.1.exon3;Parent=MsaT017433.1 |
Chr3 | CDS | 109225982 | 109226072 | 109225982 | ID=cds.MsaT017433.1;Parent=MsaT017433.1 |
Chr3 | exon | 109226253 | 109226330 | 109226253 | ID=MsaT017433.1.exon2;Parent=MsaT017433.1 |
Chr3 | CDS | 109226253 | 109226330 | 109226253 | ID=cds.MsaT017433.1;Parent=MsaT017433.1 |
Chr3 | exon | 109226844 | 109226889 | 109226844 | ID=MsaT017433.1.exon1;Parent=MsaT017433.1 |
Chr3 | CDS | 109226844 | 109226889 | 109226844 | ID=cds.MsaT017433.1;Parent=MsaT017433.1 |
Gene Sequence |
Protein sequence |