Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017446 | XP_039690983.1 | 60.435 | 230 | 50 | 6 | 9 | 199 | 59 | 286 | 6.51e-75 | 241 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017446 | tr|A0A072U7R3|A0A072U7R3_MEDTR | 60.435 | 230 | 50 | 6 | 9 | 199 | 23 | 250 | 3.81e-75 | 239 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000045 | MsaG017446 | 0.815839 | 1.304330e-51 | 2.575532e-49 |
| MsaG000083 | MsaG017446 | 0.834066 | 6.749602e-56 | 2.198535e-53 |
| MsaG000113 | MsaG017446 | 0.836608 | 1.549959e-56 | 5.448444e-54 |
| MsaG000117 | MsaG017446 | 0.824645 | 1.278607e-53 | 3.185135e-51 |
| MsaG000128 | MsaG017446 | 0.826547 | 4.550422e-54 | 1.194788e-51 |
| MsaG000151 | MsaG017446 | 0.843923 | 1.942520e-58 | 8.591350e-56 |
| MsaG000153 | MsaG017446 | 0.831652 | 2.669149e-55 | 8.101813e-53 |
| MsaG000163 | MsaG017446 | 0.840689 | 1.383931e-57 | 5.520506e-55 |
| MsaG000179 | MsaG017446 | 0.883541 | 8.799847e-71 | 1.878358e-67 |
| MsaG000207 | MsaG017446 | 0.868530 | 1.233547e-65 | 1.344881e-62 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017446 | MtrunA17_Chr1g0203051 | 51.316 | 228 | 72 | 4 | 14 | 202 | 20 | 247 | 1.13e-63 | 211 |
| MsaG017446 | MtrunA17_Chr8g0389811 | 39.007 | 141 | 52 | 4 | 47 | 156 | 58 | 195 | 2.02e-26 | 103 |
| MsaG017446 | MtrunA17_Chr2g0331371 | 32.000 | 125 | 57 | 3 | 54 | 150 | 36 | 160 | 2.04e-16 | 76.3 |
| MsaG017446 | MtrunA17_Chr1g0171741 | 29.442 | 197 | 104 | 6 | 9 | 175 | 71 | 262 | 3.89e-15 | 73.6 |
| MsaG017446 | MtrunA17_Chr2g0287651 | 25.703 | 249 | 137 | 7 | 4 | 207 | 3 | 248 | 2.67e-13 | 70.1 |
| MsaG017446 | MtrunA17_Chr7g0228051 | 51.471 | 68 | 30 | 1 | 18 | 82 | 18 | 85 | 1.48e-12 | 62.4 |
| MsaG017446 | MtrunA17_Chr8g0340801 | 50.847 | 59 | 18 | 1 | 156 | 203 | 1 | 59 | 2.88e-12 | 62.0 |
Find 56 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTCAAGTACACGCTGAT+TGG | 0.456960 | 3:-109337408 | MsaT017446.1:CDS |
| AACCAGCATAAAATCTACCA+CGG | 0.675988 | 3:+109336528 | None:intergenic |
| AACTAGTAGGTGTGGTTGCA+TGG | 0.492607 | 3:-109337434 | MsaT017446.1:CDS |
| AAGTTCAAACGGTGTATGTT+CGG | 0.449433 | 3:-109336673 | MsaT017446.1:CDS |
| AATACCAAATGAAAATGATG+AGG | 0.636392 | 3:-109337276 | MsaT017446.1:CDS |
| AATGTCTTGTGATGGAACGA+TGG | 0.702239 | 3:-109336047 | MsaT017446.1:CDS |
| AATTTGTCCCGAAGTTCAAA+CGG | 0.526146 | 3:-109336684 | MsaT017446.1:intron |
| ACAACGAGCAATTCAAGAAC+AGG | 0.501621 | 3:-109337166 | MsaT017446.1:CDS |
| ACATGTACAGTTCGTTGTAT+GGG | 0.550841 | 3:-109337733 | MsaT017446.1:CDS |
| ACCTTCTCTATGACAAACAA+AGG | 0.615884 | 3:+109337501 | None:intergenic |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 109336036 | 109337988 | 109336036 | ID=MsaG017446 |
| Chr3 | mRNA | 109336036 | 109337988 | 109336036 | ID=MsaT017446.1;Parent=MsaG017446 |
| Chr3 | exon | 109336036 | 109336097 | 109336036 | ID=MsaT017446.1.exon7;Parent=MsaT017446.1 |
| Chr3 | CDS | 109336036 | 109336097 | 109336036 | ID=cds.MsaT017446.1;Parent=MsaT017446.1 |
| Chr3 | exon | 109336512 | 109336692 | 109336512 | ID=MsaT017446.1.exon6;Parent=MsaT017446.1 |
| Chr3 | CDS | 109336512 | 109336692 | 109336512 | ID=cds.MsaT017446.1;Parent=MsaT017446.1 |
| Chr3 | exon | 109337157 | 109337229 | 109337157 | ID=MsaT017446.1.exon5;Parent=MsaT017446.1 |
| Chr3 | CDS | 109337157 | 109337229 | 109337157 | ID=cds.MsaT017446.1;Parent=MsaT017446.1 |
| Chr3 | exon | 109337259 | 109337458 | 109337259 | ID=MsaT017446.1.exon4;Parent=MsaT017446.1 |
| Chr3 | CDS | 109337259 | 109337458 | 109337259 | ID=cds.MsaT017446.1;Parent=MsaT017446.1 |
| Chr3 | exon | 109337489 | 109337545 | 109337489 | ID=MsaT017446.1.exon3;Parent=MsaT017446.1 |
| Chr3 | CDS | 109337489 | 109337545 | 109337489 | ID=cds.MsaT017446.1;Parent=MsaT017446.1 |
| Chr3 | exon | 109337586 | 109337816 | 109337586 | ID=MsaT017446.1.exon2;Parent=MsaT017446.1 |
| Chr3 | CDS | 109337586 | 109337816 | 109337586 | ID=cds.MsaT017446.1;Parent=MsaT017446.1 |
| Chr3 | exon | 109337935 | 109337988 | 109337935 | ID=MsaT017446.1.exon1;Parent=MsaT017446.1 |
| Chr3 | CDS | 109337935 | 109337988 | 109337935 | ID=cds.MsaT017446.1;Parent=MsaT017446.1 |
| Gene Sequence |
>MsaT017446.1 ATGTGTTTACATAATCGGGGATGCAACCTTAATGCGGAAGGTTCAGAGGTAGAGGACATGGACAATAGCTCTTGTTCGGAGAGTGATGTATGTTCCTCGGATGAAGATGTCGAAGACATGTACAGTTCGTTGTATGGGTGCAACTTTGACGATGAAACGGCGGTTGATTGCATGGCTGACCTTGACAGAATTAATTTTAAGGAAATAGGGTCGGATGAAATCATGCGGTACCATTTTCAGGATCGTTTAGTTGCATTCAAGTTTTACAATTTATGCGGTTGGTACGATGGCGATGTTGTTCAACAAACCTTTGTTTGTCATAGAGAAGGTTACAGGTTGGAAGCAACTAGTAGGTGTGGTTGCATGGCAAAAATTCAAGTACACGCTGATTGGTATGGTCGATGGTACATAAAATCAATTGACGATATTCACAATCACACCTTTTTGGATGGGAAGTATCAGGGAATGCTACCTGCACATAGGAAGATGAGCGATTATGACAAATACCAAATGAAAATGATGAGGAAAGTTGGCATTCCTACTGGTGGATTTAAAAATCTTGGATATACAAGAAGAGACATGTACAACGAGCAATTCAAGAACAGGAGAGGAAGATTCAAACGGTGTATGTTCGGTGATTTTGATATTGAAGAGTTTGAAACTATGTGGGATTTGTTGGTTGCAGAGTTTGGGTTGGAAAATAATAAGTGGATTCGTGAGCTGTATGAGAAGCGGAGCAATGGTCAACTGCCCATATCCGTGGTAGATTTTATGCTGGTTTCAGAACAACCTCAAGTGCATTTGAACATTGTTGTCTCGCTGCGAATGTCTTGTGATGGAACGATGGACCAAACGTAA |
| Protein sequence |
>MsaT017446.1 MCLHNRGCNLNAEGSEVEDMDNSSCSESDVCSSDEDVEDMYSSLYGCNFDDETAVDCMADLDRINFKEIGSDEIMRYHFQDRLVAFKFYNLCGWYDGDVVQQTFVCHREGYRLEATSRCGCMAKIQVHADWYGRWYIKSIDDIHNHTFLDGKYQGMLPAHRKMSDYDKYQMKMMRKVGIPTGGFKNLGYTRRDMYNEQFKNRRGRFKRCMFGDFDIEEFETMWDLLVAEFGLENNKWIRELYEKRSNGQLPISVVDFMLVSEQPQVHLNIVVSLRMSCDGTMDQT* |
