AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017453

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017453 MtrunA17_Chr3g0140431 99.666 598 2 0 88 685 1 598 0.0 1258
MsaG017453 MtrunA17_Chr1g0146301 79.360 688 132 5 2 685 3 684 0.0 1126
MsaG017453 MtrunA17_Chr7g0214531 78.130 599 121 2 92 684 4 598 0.0 1018
MsaG017453 MtrunA17_Chr8g0340491 77.961 608 123 3 85 685 88 691 0.0 986
MsaG017453 MtrunA17_Chr1g0213321 74.590 610 144 4 82 684 70 675 0.0 977
MsaG017453 MtrunA17_Chr7g0253211 74.958 595 140 2 95 684 294 884 0.0 966
MsaG017453 MtrunA17_Chr4g0071031 39.927 273 153 5 125 394 9 273 1.15e-59 207
MsaG017453 MtrunA17_Chr2g0329981 38.828 273 156 5 125 394 9 273 2.28e-58 204
MsaG017453 MtrunA17_Chr2g0299661 38.828 273 156 5 125 394 9 273 1.37e-57 202
MsaG017453 MtrunA17_Chr1g0166821 38.434 281 160 6 117 394 4 274 1.40e-56 199
MsaG017453 MtrunA17_Chr8g0342821 38.951 267 151 6 125 388 9 266 1.89e-56 199
MsaG017453 MtrunA17_Chr7g0261501 38.828 273 156 5 125 394 9 273 1.90e-56 199
MsaG017453 MtrunA17_Chr1g0187571 38.710 279 158 6 119 394 6 274 1.52e-55 196
MsaG017453 MtrunA17_Chr1g0183631 37.276 279 162 6 119 394 5 273 3.92e-54 192
MsaG017453 MtrunA17_Chr1g0167261 38.163 283 160 6 117 394 4 276 1.57e-53 187
MsaG017453 MtrunA17_Chr8g0390211 37.993 279 160 6 119 394 5 273 3.56e-53 189
MsaG017453 MtrunA17_Chr1g0146151 37.102 283 164 7 115 394 2 273 4.57e-53 188
MsaG017453 MtrunA17_Chr6g0486611 36.630 273 162 5 125 394 9 273 1.04e-52 187
MsaG017453 MtrunA17_Chr3g0140071 36.201 279 165 6 119 394 5 273 2.14e-52 187
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017453 AT2G25760.1 76.046 693 138 7 1 685 1 673 0.0 1098
MsaG017453 AT2G25760.2 75.431 696 140 8 1 685 1 676 0.0 1088
MsaG017453 AT3G13670.1 70.360 695 183 6 1 685 22 703 0.0 1020
MsaG017453 AT3G03940.1 73.770 610 149 3 82 684 95 700 0.0 969
MsaG017453 AT5G18190.2 73.927 606 147 3 86 684 89 690 0.0 966
MsaG017453 AT5G18190.1 73.927 606 147 3 86 684 89 690 0.0 966
MsaG017453 AT3G03930.1 69.388 245 74 1 441 684 43 287 2.43e-121 363
MsaG017453 AT4G26100.1 39.194 273 155 5 125 394 9 273 1.63e-58 204
MsaG017453 AT2G19470.1 39.560 273 154 5 125 394 9 273 6.94e-58 202
MsaG017453 AT3G23340.1 39.427 279 156 6 119 394 5 273 6.95e-58 202
MsaG017453 AT1G03930.1 38.790 281 159 6 117 394 3 273 8.81e-58 203
MsaG017453 AT1G72710.1 39.194 273 154 6 125 394 9 272 9.27e-57 200
MsaG017453 AT5G44100.1 37.367 281 163 6 117 394 3 273 1.26e-56 200
MsaG017453 AT5G44100.2 37.367 281 163 6 117 394 3 273 1.26e-56 200
MsaG017453 AT5G57015.1 38.462 273 157 5 125 394 9 273 1.28e-56 199
MsaG017453 AT4G14340.2 39.068 279 157 6 119 394 11 279 1.69e-56 196
MsaG017453 AT4G14340.1 39.068 279 157 6 119 394 11 279 3.04e-56 198
MsaG017453 AT4G28540.1 37.993 279 160 6 119 394 9 277 1.40e-55 197
MsaG017453 AT5G43320.1 39.068 279 157 6 119 394 5 273 1.75e-55 197
MsaG017453 AT5G43320.2 39.068 279 157 6 119 394 5 273 1.11e-54 192
MsaG017453 AT5G43320.3 39.068 279 157 6 119 394 5 273 1.11e-54 192
MsaG017453 AT4G28860.2 37.993 279 160 6 119 394 5 273 1.86e-54 191
MsaG017453 AT1G04440.1 37.993 279 160 6 119 394 5 273 2.02e-54 194
MsaG017453 AT4G28860.1 37.993 279 160 6 119 394 5 273 5.90e-54 191
MsaG017453 AT4G28880.3 38.351 279 159 6 119 394 5 273 8.88e-54 191
MsaG017453 AT4G28880.2 38.351 279 159 6 119 394 5 273 8.88e-54 191
MsaG017453 AT4G28880.1 38.351 279 159 6 119 394 5 273 8.88e-54 191
MsaG017453 AT4G28880.4 38.351 279 159 6 119 394 5 273 8.88e-54 191
MsaG017453 AT3G23340.2 38.697 261 149 5 137 394 7 259 7.02e-51 183
MsaG017453 AT4G08800.1 34.066 273 137 6 125 394 9 241 1.97e-42 155

Find 192 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAGCACCCTCGGTATTT+AGG 0.209029 3:-109389068 None:intergenic
CCCATTCGGACCTTCTTCTT+TGG 0.219272 3:-109389248 None:intergenic
GACCCACAATTCCATCAAAA+AGG 0.232071 3:-109389026 None:intergenic
CTGAGCACCCTCGGTATTTA+GGG 0.247642 3:-109389067 None:intergenic
CTTTCCCAACACATTGTAAA+AGG 0.251961 3:+109389434 MsaT017453.1:CDS
TGGCTTCTTGTGCTAGTCTT+TGG 0.253271 3:+109389486 MsaT017453.1:CDS
AAGCTTGGGAAGGGAGGATT+TGG 0.257494 3:+109386398 MsaT017453.1:CDS
TGTTTCACAGGAATGGATTA+TGG 0.259630 3:+109389702 MsaT017453.1:intron
ATCAACGCCCAGATGTATTT+AGG 0.259852 3:+109388527 MsaT017453.1:CDS
CTTCTGCCTCCGATTCCTTC+TGG 0.270294 3:-109385902 None:intergenic
AAACGAGTCACTGACTTTAT+AGG 0.282911 3:-109389959 None:intergenic
GGCTTCTTGTGCTAGTCTTT+GGG 0.284395 3:+109389487 MsaT017453.1:CDS
ATATGCTTGGCTCTAGTCTA+TGG 0.295213 3:+109387079 MsaT017453.1:CDS
TGGCCACTGCTGGCACTAGA+TGG 0.300589 3:+109390034 MsaT017453.1:CDS
TATAGGATTGTTGCATGTAT+TGG 0.306085 3:-109389942 None:intergenic
GCTCAAAACCTTTGTTTCAT+TGG 0.309003 3:-109389314 None:intergenic
TAAGAAGAAGGCAGTGGTTA+GGG 0.314293 3:+109386023 MsaT017453.1:CDS
AGGGGCCTAATGTCAGGATT+TGG 0.316161 3:-109389048 None:intergenic
GCTCTAGTCTATGGGATGTT+TGG 0.316967 3:+109387088 MsaT017453.1:CDS
TTCGGCTCCCGGAGCTTTAT+CGG 0.329884 3:-109386094 None:intergenic
ATAGGATTGTTGCATGTATT+GGG 0.335579 3:-109389941 None:intergenic
TTTCTGGTTGATCTTGGATT+AGG 0.337171 3:+109388381 MsaT017453.1:CDS
ACCAACCTTGTGAAGAAAAC+TGG 0.342759 3:-109389564 None:intergenic
TAGTCTTTGGGCACTCATTA+TGG 0.347322 3:+109389499 MsaT017453.1:CDS
GTGAATAACGACGACGAAAC+AGG 0.354236 3:+109385967 MsaT017453.1:CDS
ACACTAATCCACTGTGTTGC+TGG 0.363197 3:-109389275 None:intergenic
GTTACCTTGGCAAGGATATC+AGG 0.363733 3:+109388745 MsaT017453.1:CDS
TTCCTATCTAATCTATATAA+TGG 0.369860 3:-109386377 None:intergenic
CTCCCTTCTTCTTCGGCTCC+CGG 0.370094 3:-109386105 None:intergenic
AGAACAAACCTAAATACATC+TGG 0.381852 3:-109388535 None:intergenic
TTGTTACAGGGAGAAAATAA+AGG 0.383335 3:+109388865 MsaT017453.1:intron
GCAAGGTGACTACTACATTA+TGG 0.384482 3:+109386968 MsaT017453.1:CDS
CTCATTATGGATGCTGGCAC+TGG 0.386456 3:+109389512 MsaT017453.1:CDS
AACAGCAAAGGGTGCAATTA+CGG 0.391275 3:+109386771 MsaT017453.1:CDS
TATAGATTAGATAGGAAGCT+TGG 0.395411 3:+109386383 MsaT017453.1:CDS
TATATCGCATCTATTAGTTA+TGG 0.395955 3:+109390479 MsaT017453.1:CDS
ATAAGAAGAAGGCAGTGGTT+AGG 0.397165 3:+109386022 MsaT017453.1:CDS
AACTTCTTGCTTGGACCCTC+GGG 0.405644 3:+109388336 MsaT017453.1:CDS
CGCCGCCGGTCGCCGGCGGG+AGG 0.407045 3:+109385851 MsaT017453.1:CDS
ACGCGCCGCCGGTCGCCGGC+GGG 0.411110 3:+109385848 MsaT017453.1:CDS
GTGACTCGTTTCCATTTAAG+TGG 0.412212 3:+109389971 MsaT017453.1:CDS
AGTTGTACTCCCTTCTTCTT+CGG 0.412608 3:-109386112 None:intergenic
AAGTTGTTTCTGGTTGATCT+TGG 0.425523 3:+109388375 MsaT017453.1:CDS
CTCCATTATATAGATTAGAT+AGG 0.426261 3:+109386375 MsaT017453.1:CDS
ATATCGCATCTATTAGTTAT+GGG 0.426501 3:+109390480 MsaT017453.1:CDS
GGAATCAAAATCACCTTCTC+AGG 0.426563 3:-109386141 None:intergenic
ATTGTTATGTCCCGTGGTGC+TGG 0.431009 3:+109390060 MsaT017453.1:CDS
CGACGCGTAGGAGCTGGTTC+TGG 0.431637 3:+109386437 MsaT017453.1:CDS
AAACTTCTTGCTTGGACCCT+CGG 0.431706 3:+109388335 MsaT017453.1:CDS
GATAGGAAGCTTGGGAAGGG+AGG 0.432226 3:+109386392 MsaT017453.1:CDS
TTCTTCGTGGTCGGTTACCT+TGG 0.433456 3:+109388732 MsaT017453.1:CDS
CAAAAGCAACACAAAGCTTC+GGG 0.436504 3:-109388922 None:intergenic
AACGCGCCGCCGGTCGCCGG+CGG 0.437647 3:+109385847 MsaT017453.1:CDS
CCTTCTGGTCTGTGCGGCGG+TGG 0.439587 3:-109385887 None:intergenic
GTCACTGCTATGGCCACTGC+TGG 0.440107 3:+109390024 MsaT017453.1:CDS
TGGGTCCAAATCCTGACATT+AGG 0.442025 3:+109389043 MsaT017453.1:CDS
AGCGTTTCTTGAGTTTCATC+AGG 0.442256 3:-109390320 None:intergenic
ACGCTTGTCTTCCTTCTTCG+TGG 0.445559 3:+109388719 MsaT017453.1:CDS
TGTTGTGAAGCTTATATGTC+AGG 0.445999 3:+109389175 MsaT017453.1:intron
ACAAAAGCAACACAAAGCTT+CGG 0.446009 3:-109388923 None:intergenic
AAAATAAAAGCAGCTTGATC+AGG 0.446910 3:-109390275 None:intergenic
AAGCCTGAAAACTTCTTGCT+TGG 0.452042 3:+109388327 MsaT017453.1:CDS
GTATTTAGGGGCCTAATGTC+AGG 0.452090 3:-109389054 None:intergenic
CGTTGGCGTGATAGTTCAAC+TGG 0.453748 3:+109388487 MsaT017453.1:CDS
CTGTGCGGCGGTGGCAGCGA+CGG 0.454161 3:-109385878 None:intergenic
TTTACCTTAACATGAGTACT+AGG 0.455799 3:-109390359 None:intergenic
TATTACATCAGTGCCATAGC+TGG 0.456706 3:+109389742 MsaT017453.1:CDS
GTTGGCCGACGCGTAGGAGC+TGG 0.466210 3:+109386431 MsaT017453.1:CDS
AAAATCACCTTCTCAGGAAG+TGG 0.470398 3:-109386135 None:intergenic
CTCCTCCCGCCGGCGACCGG+CGG 0.471128 3:-109385853 None:intergenic
GCTCTCCTCCCGCCGGCGAC+CGG 0.471790 3:-109385856 None:intergenic
TGGGCACTCATTATGGATGC+TGG 0.472875 3:+109389506 MsaT017453.1:CDS
AGCTAGTAACGGGTCCTCAT+TGG 0.474983 3:+109389765 MsaT017453.1:CDS
ATCTAGGATGTCTACTGAAA+TGG 0.475531 3:+109387225 MsaT017453.1:intron
CGAGGTCGGGTACGCAACTC+AGG 0.476552 3:-109385805 None:intergenic
GATAACGCGCCGCCGGTCGC+CGG 0.478774 3:+109385844 MsaT017453.1:CDS
TCATTGGTTGTAATGTCAAA+AGG 0.480800 3:+109389781 MsaT017453.1:CDS
AGCTTGGGAAGGGAGGATTT+GGG 0.483807 3:+109386399 MsaT017453.1:CDS
CTACATATTCTACAAACTGC+CGG 0.484853 3:-109388957 None:intergenic
AACTAATAGATGCGATATAG+AGG 0.485124 3:-109390475 None:intergenic
ATTGTAAAAGGAAATGAAGA+TGG 0.488040 3:+109389446 MsaT017453.1:CDS
GGAAGACAAGCGTGTAAGCA+AGG 0.490386 3:-109388709 None:intergenic
ATAGATTAGATAGGAAGCTT+GGG 0.491550 3:+109386384 MsaT017453.1:CDS
CCGCACAGACCAGAAGGAAT+CGG 0.493586 3:+109385893 MsaT017453.1:CDS
CTCTGTAAATCCAGCACCAC+GGG 0.494784 3:-109390070 None:intergenic
TTCATCATCATCCTCTTCCA+TGG 0.494964 3:-109389222 None:intergenic
GTGTTGCTGGCTGACCCATT+CGG 0.496471 3:-109389262 None:intergenic
TTAGATAGGAAGCTTGGGAA+GGG 0.503149 3:+109386389 MsaT017453.1:CDS
CGTAGTGCCGATAAAGCTCC+GGG 0.503731 3:+109386087 MsaT017453.1:CDS
GCTCCGGGAGCCGAAGAAGA+AGG 0.504739 3:+109386102 MsaT017453.1:CDS
CCGTAGTGCCGATAAAGCTC+CGG 0.505206 3:+109386086 MsaT017453.1:CDS
GGAAGGAGAGAATAAGAAGA+AGG 0.510183 3:+109386011 MsaT017453.1:CDS
CCATAGCTGGAGCTAGTAAC+GGG 0.513636 3:+109389755 MsaT017453.1:CDS
ATTAGATAGGAAGCTTGGGA+AGG 0.514540 3:+109386388 MsaT017453.1:CDS
TTGTCTTCCTTCTTCGTGGT+CGG 0.524778 3:+109388723 MsaT017453.1:CDS
GCTATGGATGAGTACGATAG+TGG 0.525051 3:+109386060 MsaT017453.1:CDS
CTTCGATGAATACCTTCACT+AGG 0.528484 3:-109390212 None:intergenic
TTCTCATCAGGGGTTCCCGA+GGG 0.529311 3:-109388351 None:intergenic
GGATTTGGGCAAGTCTATGT+TGG 0.531558 3:+109386413 MsaT017453.1:CDS
GAATACCTTCACTAGGGTAC+AGG 0.532543 3:-109390205 None:intergenic
CCAAAGAAGAAGGTCCGAAT+GGG 0.533192 3:+109389248 MsaT017453.1:CDS
TAATTACAGATTCAAGTTGG+TGG 0.534253 3:+109386350 MsaT017453.1:intron
AGGACGATTGACCATGGAAG+AGG 0.534909 3:+109389211 MsaT017453.1:CDS
ATTACGGACCTCCACATGAA+TGG 0.537264 3:+109386787 MsaT017453.1:CDS
TCTTAATTACAGATTCAAGT+TGG 0.538540 3:+109386347 MsaT017453.1:intron
ACAAACTGCCGGAAAGGTGC+AGG 0.538874 3:-109388946 None:intergenic
TATGCTTGGCTCTAGTCTAT+GGG 0.539645 3:+109387080 MsaT017453.1:CDS
AGCTGGTTCTGGTGCCGTTG+AGG 0.543472 3:+109386448 MsaT017453.1:CDS
GGTTTCTAGAGAGGAAGCTA+TGG 0.545782 3:+109386044 MsaT017453.1:CDS
GAGACGGTTTATGCGAGGTC+GGG 0.546267 3:-109385818 None:intergenic
CGGACCTCCACATGAATGGC+AGG 0.546511 3:+109386791 MsaT017453.1:CDS
TGGTGCTGGATTTACAGAGC+AGG 0.546908 3:+109390074 MsaT017453.1:CDS
GAGAGATTGAAAGAAGAGGA+AGG 0.548043 3:+109385994 MsaT017453.1:CDS
GGCCACTGCTGGCACTAGAT+GGG 0.549197 3:+109390035 MsaT017453.1:CDS
AGAGAATAAGAAGAAGGCAG+TGG 0.549582 3:+109386017 MsaT017453.1:CDS
AAGGTAACCGACCACGAAGA+AGG 0.552994 3:-109388730 None:intergenic
GTGAAGGTATTCATCGAAGG+TGG 0.556433 3:+109390216 MsaT017453.1:CDS
TTTCTCATCAGGGGTTCCCG+AGG 0.557461 3:-109388352 None:intergenic
CTCCGGGAGCCGAAGAAGAA+GGG 0.558776 3:+109386103 MsaT017453.1:CDS
TGAGCACCCTCGGTATTTAG+GGG 0.558929 3:-109389066 None:intergenic
TGATGAACAACCAAAGAAGA+AGG 0.560213 3:+109389238 MsaT017453.1:CDS
AGTGGTTAGGGTTTCTAGAG+AGG 0.560234 3:+109386035 MsaT017453.1:CDS
AGGAATGGATTATGGAACAG+TGG 0.562856 3:+109389710 MsaT017453.1:intron
CGTGGTCGGTTACCTTGGCA+AGG 0.564377 3:+109388737 MsaT017453.1:CDS
AAATACCGAGGGTGCTCAGA+AGG 0.565005 3:+109389071 MsaT017453.1:CDS
CACGAGTACATTACAAGGGA+CGG 0.566096 3:+109386946 MsaT017453.1:CDS
AGTAGAGAGATTGAAAGAAG+AGG 0.566314 3:+109385990 MsaT017453.1:CDS
GCTAGTGTGCATGCACATCT+TGG 0.567152 3:+109388653 MsaT017453.1:CDS
AAGTAGCAGGAGAGATGACT+TGG 0.570146 3:+109388682 MsaT017453.1:CDS
TAGAGAAGATGCATTGTAGA+GGG 0.572443 3:+109387281 MsaT017453.1:CDS
CCACCGCCGCACAGACCAGA+AGG 0.573426 3:+109385887 MsaT017453.1:CDS
ACCAAAGAAGAAGGTCCGAA+TGG 0.576715 3:+109389247 MsaT017453.1:CDS
TATTCTACAAACTGCCGGAA+AGG 0.578047 3:-109388952 None:intergenic
GTTTCTTGAGTTTCATCAGG+TGG 0.578812 3:-109390317 None:intergenic
ACACAAAGCTTCGGGTGAAG+TGG 0.580070 3:-109388914 None:intergenic
ACACAGGTCATGGATATGCT+TGG 0.581027 3:+109387066 MsaT017453.1:intron
GCCATAGCTGGAGCTAGTAA+CGG 0.586802 3:+109389754 MsaT017453.1:CDS
GAACAAACCTAAATACATCT+GGG 0.586854 3:-109388534 None:intergenic
TGTTCCACGAGTACATTACA+AGG 0.586861 3:+109386941 MsaT017453.1:CDS
GGAATGGATTATGGAACAGT+GGG 0.588580 3:+109389711 MsaT017453.1:intron
GTCTATGTTGGCCGACGCGT+AGG 0.589738 3:+109386425 MsaT017453.1:CDS
TACAACTCCACTTCCTGAGA+AGG 0.591492 3:+109386128 MsaT017453.1:CDS
GTGTTGGGAAAGCCGCTCAT+CGG 0.591532 3:-109389423 None:intergenic
ATCTTGGTAGAACAAGTAGC+AGG 0.592624 3:+109388669 MsaT017453.1:CDS
TTAGAGAAGATGCATTGTAG+AGG 0.593386 3:+109387280 MsaT017453.1:CDS
TGAGACGGTTTATGCGAGGT+CGG 0.596794 3:-109385819 None:intergenic
AGCACATTAGGTGGTAGTCA+TGG 0.600563 3:+109386918 MsaT017453.1:intron
TCTCACTGATAACGCGCCGC+CGG 0.600651 3:+109385837 MsaT017453.1:CDS
AGCGACGGCTCTCCTCCCGC+CGG 0.600658 3:-109385863 None:intergenic
ATAACCTGATATCCTTGCCA+AGG 0.602758 3:-109388749 None:intergenic
GTTGATCATAATCAACATGA+AGG 0.605161 3:-109388510 None:intergenic
GATTTCCTGTACCCTAGTGA+AGG 0.606757 3:+109390200 MsaT017453.1:CDS
CACAGACCAGAAGGAATCGG+AGG 0.609955 3:+109385896 MsaT017453.1:CDS
TGAAGGTATTCATCGAAGGT+GGG 0.610628 3:+109390217 MsaT017453.1:CDS
CTCGTAGACCAATGAAACAA+AGG 0.611155 3:+109389306 MsaT017453.1:CDS
TTGTAAACCTGCCATTCATG+TGG 0.611605 3:-109386798 None:intergenic
TAGGATGTCTACTGAAATGG+TGG 0.612266 3:+109387228 MsaT017453.1:intron
TTGAGCATAGAAACAGCAAA+GGG 0.612579 3:+109386760 MsaT017453.1:CDS
CCCGTTACTAGCTCCAGCTA+TGG 0.614186 3:-109389755 None:intergenic
TTTGAGCATAGAAACAGCAA+AGG 0.615974 3:+109386759 MsaT017453.1:CDS
AAGTACCTTCTGAGCACCCT+CGG 0.619914 3:-109389076 None:intergenic
AAAGAGAGGACGATTGACCA+TGG 0.622958 3:+109389205 MsaT017453.1:CDS
ATTCCTTCTGGTCTGTGCGG+CGG 0.623393 3:-109385890 None:intergenic
TTCGATGAATACCTTCACTA+GGG 0.624580 3:-109390211 None:intergenic
CGTCCCTTGTAATGTACTCG+TGG 0.624983 3:-109386945 None:intergenic
GGTGGCATGTATTGCAATCG+AGG 0.627258 3:+109387246 MsaT017453.1:CDS
CCGGAGCTTTATCGGCACTA+CGG 0.627559 3:-109386086 None:intergenic
TAAACCTGCCATTCATGTGG+AGG 0.628206 3:-109386795 None:intergenic
GTACATTACAAGGGACGGCA+AGG 0.629460 3:+109386951 MsaT017453.1:CDS
GTGAAGCTTATATGTCAGGT+TGG 0.629643 3:+109389179 MsaT017453.1:intron
ATTACAATGTGGCCGATGAG+CGG 0.631315 3:+109389411 MsaT017453.1:CDS
TGCCCATCTAGTGCCAGCAG+TGG 0.632198 3:-109390037 None:intergenic
GCGGCGCGTTATCAGTGAGA+CGG 0.633560 3:-109385834 None:intergenic
TGTTGTTGCAGATATGTGCA+TGG 0.634497 3:+109388297 MsaT017453.1:intron
GAAGACAAGCGTGTAAGCAA+GGG 0.638778 3:-109388708 None:intergenic
TTGCAGCAGTTGCAGTAATG+CGG 0.640610 3:-109390250 None:intergenic
CTAGTGAAGGTATTCATCGA+AGG 0.644295 3:+109390213 MsaT017453.1:CDS
TGGGCAATTGTTATGTCCCG+TGG 0.648130 3:+109390054 MsaT017453.1:CDS
TACTAGGAAAATCAGACGTG+CGG 0.651938 3:-109390343 None:intergenic
TGGTTTATTTATTAGCAGTG+TGG 0.653080 3:+109389466 MsaT017453.1:CDS
AAACACCGTACATACCTCAA+CGG 0.667251 3:-109386462 None:intergenic
TGTCAGGTTGGACAAAAGAG+AGG 0.668670 3:+109389191 MsaT017453.1:CDS
ATGGATGAGTACGATAGTGG+TGG 0.670453 3:+109386063 MsaT017453.1:CDS
CAGAACCAGCTCCTACGCGT+CGG 0.671406 3:-109386436 None:intergenic
GCTCTGTAAATCCAGCACCA+CGG 0.673158 3:-109390071 None:intergenic
CATTAGGCCCCTAAATACCG+AGG 0.680909 3:+109389059 MsaT017453.1:CDS
TCAGTGAGACGGTTTATGCG+AGG 0.681265 3:-109385823 None:intergenic
ATTAGGCCCCTAAATACCGA+GGG 0.697940 3:+109389060 MsaT017453.1:CDS
TGGGTCAGCCAGCAACACAG+TGG 0.714696 3:+109389267 MsaT017453.1:CDS
GCGTAGGTATCATTACAATG+TGG 0.721199 3:+109389400 MsaT017453.1:intron
GTTCCACGAGTACATTACAA+GGG 0.736863 3:+109386942 MsaT017453.1:CDS
CCGATTCCTTCTGGTCTGTG+CGG 0.742167 3:-109385893 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 109385802 109390517 109385802 ID=MsaG017453
Chr3 mRNA 109385802 109390517 109385802 ID=MsaT017453.1;Parent=MsaG017453
Chr3 exon 109385802 109386149 109385802 ID=MsaT017453.1.exon1;Parent=MsaT017453.1
Chr3 CDS 109385802 109386149 109385802 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109386359 109386469 109386359 ID=MsaT017453.1.exon2;Parent=MsaT017453.1
Chr3 CDS 109386359 109386469 109386359 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109386747 109386820 109386747 ID=MsaT017453.1.exon3;Parent=MsaT017453.1
Chr3 CDS 109386747 109386820 109386747 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109386920 109386989 109386920 ID=MsaT017453.1.exon4;Parent=MsaT017453.1
Chr3 CDS 109386920 109386989 109386920 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109387072 109387127 109387072 ID=MsaT017453.1.exon5;Parent=MsaT017453.1
Chr3 CDS 109387072 109387127 109387072 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109387231 109387302 109387231 ID=MsaT017453.1.exon6;Parent=MsaT017453.1
Chr3 CDS 109387231 109387302 109387231 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109388308 109388402 109388308 ID=MsaT017453.1.exon7;Parent=MsaT017453.1
Chr3 CDS 109388308 109388402 109388308 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109388482 109388548 109388482 ID=MsaT017453.1.exon8;Parent=MsaT017453.1
Chr3 CDS 109388482 109388548 109388482 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109388637 109388766 109388637 ID=MsaT017453.1.exon9;Parent=MsaT017453.1
Chr3 CDS 109388637 109388766 109388637 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109388874 109389092 109388874 ID=MsaT017453.1.exon10;Parent=MsaT017453.1
Chr3 CDS 109388874 109389092 109388874 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109389185 109389327 109389185 ID=MsaT017453.1.exon11;Parent=MsaT017453.1
Chr3 CDS 109389185 109389327 109389185 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109389406 109389580 109389406 ID=MsaT017453.1.exon12;Parent=MsaT017453.1
Chr3 CDS 109389406 109389580 109389406 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109389712 109389802 109389712 ID=MsaT017453.1.exon13;Parent=MsaT017453.1
Chr3 CDS 109389712 109389802 109389712 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109389938 109390095 109389938 ID=MsaT017453.1.exon14;Parent=MsaT017453.1
Chr3 CDS 109389938 109390095 109389938 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109390188 109390376 109390188 ID=MsaT017453.1.exon15;Parent=MsaT017453.1
Chr3 CDS 109390188 109390376 109390188 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Chr3 exon 109390458 109390517 109390458 ID=MsaT017453.1.exon16;Parent=MsaT017453.1
Chr3 CDS 109390458 109390517 109390458 ID=cds.MsaT017453.1;Parent=MsaT017453.1
Gene Sequence

>MsaT017453.1

ATGCCTGAGTTGCGTACCCGACCTCGCATAAACCGTCTCACTGATAACGCGCCGCCGGTCGCCGGCGGGAGGAGAGCCGTCGCTGCCACCGCCGCACAGACCAGAAGGAATCGGAGGCAGAAGAAGAAGAAGAACACGATCGTTGCCGAGAAAATCGAAAACATTGTGAATAACGACGACGAAACAGGAGTAGAGAGATTGAAAGAAGAGGAAGGAGAGAATAAGAAGAAGGCAGTGGTTAGGGTTTCTAGAGAGGAAGCTATGGATGAGTACGATAGTGGTGGCCGTAGTGCCGATAAAGCTCCGGGAGCCGAAGAAGAAGGGAGTACAACTCCACTTCCTGAGAAGATTCAAGTTGGTGGTTCTCCATTATATAGATTAGATAGGAAGCTTGGGAAGGGAGGATTTGGGCAAGTCTATGTTGGCCGACGCGTAGGAGCTGGTTCTGGTGCCGTTGAGGTAGCATTAAAATTTGAGCATAGAAACAGCAAAGGGTGCAATTACGGACCTCCACATGAATGGCAGGTTTACAACACATTAGGTGGTAGTCATGGTGTTCCACGAGTACATTACAAGGGACGGCAAGGTGACTACTACATTATGGTCATGGATATGCTTGGCTCTAGTCTATGGGATGTTTGGAATAACAACTCACACACGATGTCTACTGAAATGGTGGCATGTATTGCAATCGAGGCTATCTCTATATTAGAGAAGATGCATTGTAGAGGATATGTGCATGGTGATGTAAAGCCTGAAAACTTCTTGCTTGGACCCTCGGGAACCCCTGATGAGAAAAAGTTGTTTCTGGTTGATCTTGGATTAGCAACTCGTTGGCGTGATAGTTCAACTGGCCTTCATGTTGATTATGATCAACGCCCAGATGTATTTAGGGGAACAGTTCGTTATGCTAGTGTGCATGCACATCTTGGTAGAACAAGTAGCAGGAGAGATGACTTGGAATCCCTTGCTTACACGCTTGTCTTCCTTCTTCGTGGTCGGTTACCTTGGCAAGGATATCAGGGAGAAAATAAAGGATTTCTTGTCTGCAAGAAAAAGATGGCCACTTCACCCGAAGCTTTGTGTTGCTTTTGTCCTGCACCTTTCCGGCAGTTTGTAGAATATGTAGTAAATTTGAAGTTTGATGAAGAACCAAATTATGCAAAATACATATCCCTTTTTGATGGAATTGTGGGTCCAAATCCTGACATTAGGCCCCTAAATACCGAGGGTGCTCAGAAGCTTATATGTCAGGTTGGACAAAAGAGAGGACGATTGACCATGGAAGAGGATGATGATGAACAACCAAAGAAGAAGGTCCGAATGGGTCAGCCAGCAACACAGTGGATTAGTGTTTACAATGCTCGTAGACCAATGAAACAAAGGTATCATTACAATGTGGCCGATGAGCGGCTTTCCCAACACATTGTAAAAGGAAATGAAGATGGTTTATTTATTAGCAGTGTGGCTTCTTGTGCTAGTCTTTGGGCACTCATTATGGATGCTGGCACTGGTTTCACATCACAAGTTTACGAACTCTCTTCCAGTTTTCTTCACAAGGAATGGATTATGGAACAGTGGGATAAGAATTATTACATCAGTGCCATAGCTGGAGCTAGTAACGGGTCCTCATTGGTTGTAATGTCAAAAGGGACCCAATACATGCAACAATCCTATAAAGTCAGTGACTCGTTTCCATTTAAGTGGATCAACAAAAAATGGAGGGAAGGATTTTTTGTCACTGCTATGGCCACTGCTGGCACTAGATGGGCAATTGTTATGTCCCGTGGTGCTGGATTTACAGAGCAGGTTGTGGAACTTGATTTCCTGTACCCTAGTGAAGGTATTCATCGAAGGTGGGAAAACAATTACCGCATTACTGCAACTGCTGCAACGCCTGATCAAGCTGCTTTTATTTTAAGTGTCCCAAGAAGAAAACCACCTGATGAAACTCAAGAAACGCTCCGCACGTCTGATTTTCCTAGTACTCATGTTAAGGAAAAATGGGCAAAGAACCTCTATATCGCATCTATTAGTTATGGGAGAACAGTTTCATAA
Protein sequence

>MsaT017453.1

MPELRTRPRINRLTDNAPPVAGGRRAVAATAAQTRRNRRQKKKKNTIVAEKIENIVNNDDETGVERLKEEEGENKKKAVVRVSREEAMDEYDSGGRSADKAPGAEEEGSTTPLPEKIQVGGSPLYRLDRKLGKGGFGQVYVGRRVGAGSGAVEVALKFEHRNSKGCNYGPPHEWQVYNTLGGSHGVPRVHYKGRQGDYYIMVMDMLGSSLWDVWNNNSHTMSTEMVACIAIEAISILEKMHCRGYVHGDVKPENFLLGPSGTPDEKKLFLVDLGLATRWRDSSTGLHVDYDQRPDVFRGTVRYASVHAHLGRTSSRRDDLESLAYTLVFLLRGRLPWQGYQGENKGFLVCKKKMATSPEALCCFCPAPFRQFVEYVVNLKFDEEPNYAKYISLFDGIVGPNPDIRPLNTEGAQKLICQVGQKRGRLTMEEDDDEQPKKKVRMGQPATQWISVYNARRPMKQRYHYNVADERLSQHIVKGNEDGLFISSVASCASLWALIMDAGTGFTSQVYELSSSFLHKEWIMEQWDKNYYISAIAGASNGSSLVVMSKGTQYMQQSYKVSDSFPFKWINKKWREGFFVTAMATAGTRWAIVMSRGAGFTEQVVELDFLYPSEGIHRRWENNYRITATAATPDQAAFILSVPRRKPPDETQETLRTSDFPSTHVKEKWAKNLYIASISYGRTVS*