Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017545 | XP_013462127.2 | 98.913 | 92 | 1 | 0 | 1 | 92 | 1 | 92 | 1.39e-60 | 189 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017545 | sp|F4JVB8|RADL1_ARATH | 47.778 | 90 | 44 | 2 | 3 | 92 | 14 | 100 | 7.73e-25 | 92.4 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017545 | tr|A0A396IZP4|A0A396IZP4_MEDTR | 98.913 | 92 | 1 | 0 | 1 | 92 | 1 | 92 | 6.62e-61 | 189 |
Gene ID | Type | Classification |
---|---|---|
MsaG017545 | TF | MYB-related |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000216 | MsaG017545 | 0.802198 | 1.055197e-48 | 1.499478e-46 |
MsaG000350 | MsaG017545 | 0.809262 | 3.522560e-50 | 5.908962e-48 |
MsaG000510 | MsaG017545 | 0.803440 | 5.862685e-49 | 8.572535e-47 |
MsaG000719 | MsaG017545 | 0.800787 | 2.047459e-48 | 2.817984e-46 |
MsaG005130 | MsaG017545 | 0.833157 | 1.135406e-55 | 3.601115e-53 |
MsaG005288 | MsaG017545 | 0.802436 | 9.431937e-49 | 1.347667e-46 |
MsaG007497 | MsaG017545 | 0.827390 | 2.867735e-54 | 7.709181e-52 |
MsaG007607 | MsaG017545 | 0.812717 | 6.336590e-51 | 1.156756e-48 |
MsaG009256 | MsaG017545 | 0.802552 | 8.931143e-49 | 1.279523e-46 |
MsaG011133 | MsaG017545 | 0.814956 | 2.046089e-51 | 3.950912e-49 |
MsaG012393 | MsaG017545 | 0.857933 | 2.272134e-62 | 1.634319e-59 |
MsaG013265 | MsaG017545 | 0.815979 | 1.214365e-51 | 2.406458e-49 |
MsaG014548 | MsaG017545 | 0.834199 | 6.253869e-56 | 2.045221e-53 |
MsaG014720 | MsaG017545 | 0.849131 | 7.469897e-60 | 3.930402e-57 |
MsaG015347 | MsaG017545 | 0.855392 | 1.258937e-61 | 8.249422e-59 |
MsaG017362 | MsaG017545 | 0.828855 | 1.276805e-54 | 3.576324e-52 |
MsaG017484 | MsaG017545 | 0.841630 | 7.847818e-58 | 3.225266e-55 |
MsaG017545 | MsaG017879 | 0.878310 | 6.531013e-69 | 1.090282e-65 |
MsaG017545 | MsaG019532 | 0.810750 | 1.689997e-50 | 2.939247e-48 |
MsaG017545 | MsaG019623 | 0.821996 | 5.279942e-53 | 1.224331e-50 |
MsaG017545 | MsaG020297 | 0.855183 | 1.447112e-61 | 9.410688e-59 |
MsaG017545 | MsaG020513 | 0.811556 | 1.132025e-50 | 2.008124e-48 |
MsaG017545 | MsaG022018 | 0.800913 | 1.930463e-48 | 2.664364e-46 |
MsaG017545 | MsaG022793 | 0.823436 | 2.448897e-53 | 5.903533e-51 |
MsaG017545 | MsaG022907 | 0.818896 | 2.695352e-52 | 5.758061e-50 |
MsaG017545 | MsaG023069 | 0.806240 | 1.534649e-49 | 2.395209e-47 |
MsaG017545 | MsaG023671 | 0.828089 | 1.951144e-54 | 5.348980e-52 |
MsaG017545 | MsaG023978 | 0.824292 | 1.546876e-53 | 3.816726e-51 |
MsaG017545 | MsaG024380 | 0.801414 | 1.526494e-48 | 2.130888e-46 |
MsaG017545 | MsaG027474 | 0.884358 | 4.409149e-71 | 9.788213e-68 |
MsaG017545 | MsaG027566 | 0.803775 | 5.000370e-49 | 7.368507e-47 |
MsaG017545 | MsaG030454 | 0.857152 | 3.858290e-62 | 2.695666e-59 |
MsaG017545 | MsaG030518 | 0.800603 | 2.231319e-48 | 3.058312e-46 |
MsaG017545 | MsaG031752 | 0.809396 | 3.296693e-50 | 5.548341e-48 |
MsaG017545 | MsaG032230 | 0.809872 | 2.608700e-50 | 4.441091e-48 |
MsaG017545 | MsaG032378 | 0.846298 | 4.462673e-59 | 2.134649e-56 |
MsaG017545 | MsaG032814 | 0.806257 | 1.521528e-49 | 2.375753e-47 |
MsaG017545 | MsaG033171 | 0.859812 | 6.266706e-63 | 4.837390e-60 |
MsaG017545 | MsaG033856 | 0.819132 | 2.382855e-52 | 5.122390e-50 |
MsaG017545 | MsaG033860 | 0.809088 | 3.836418e-50 | 6.408949e-48 |
MsaG017545 | MsaG036699 | 0.808558 | 4.974803e-50 | 8.205141e-48 |
MsaG017545 | MsaG038460 | 0.867852 | 2.034077e-65 | 2.156681e-62 |
MsaG017545 | MsaG039743 | 0.814136 | 3.100520e-51 | 5.864640e-49 |
MsaG017545 | MsaG040153 | 0.801148 | 1.728652e-48 | 2.398589e-46 |
MsaG017545 | MsaG041798 | 0.859493 | 7.805612e-63 | 5.953025e-60 |
MsaG017545 | MsaG003580 | 0.803071 | 6.985377e-49 | 1.012747e-46 |
MsaG017545 | MsaG004309 | 0.866511 | 5.426448e-65 | 5.447050e-62 |
MsaG017545 | MsaG009684 | 0.821473 | 6.965495e-53 | 1.592849e-50 |
MsaG017545 | MsaG007989 | 0.866612 | 5.041279e-65 | 5.082420e-62 |
MsaG017545 | MsaG031318 | 0.806200 | 1.563863e-49 | 2.438550e-47 |
MsaG017545 | MsaG031852 | 0.848616 | 1.036747e-59 | 5.359986e-57 |
MsaG017545 | MsaG032515 | 0.818043 | 4.197450e-52 | 8.770700e-50 |
MsaG017545 | MsaG034453 | 0.850302 | 3.531667e-60 | 1.933788e-57 |
MsaG017545 | MsaG036456 | 0.845500 | 7.332568e-59 | 3.415618e-56 |
MsaG017545 | MsaG037375 | 0.809230 | 3.578221e-50 | 5.997691e-48 |
MsaG017545 | MsaG046971 | 0.862815 | 7.688817e-64 | 6.662390e-61 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017545 | MtrunA17_Chr3g0141641 | 98.913 | 92 | 1 | 0 | 1 | 92 | 1 | 92 | 1.28e-64 | 189 |
MsaG017545 | MtrunA17_Chr1g0155181 | 50.000 | 84 | 39 | 1 | 2 | 85 | 6 | 86 | 6.48e-27 | 94.4 |
MsaG017545 | MtrunA17_Chr3g0141601 | 43.333 | 90 | 46 | 1 | 3 | 92 | 7 | 91 | 8.68e-23 | 84.0 |
MsaG017545 | MtrunA17_Chr7g0255841 | 44.828 | 87 | 39 | 1 | 6 | 92 | 15 | 92 | 5.48e-22 | 82.0 |
MsaG017545 | MtrunA17_Chr3g0144631 | 53.846 | 65 | 30 | 0 | 3 | 67 | 16 | 80 | 6.89e-22 | 81.3 |
MsaG017545 | MtrunA17_Chr8g0373131 | 52.632 | 76 | 31 | 2 | 1 | 76 | 3 | 73 | 2.81e-21 | 79.7 |
MsaG017545 | MtrunA17_Chr8g0373111 | 54.412 | 68 | 30 | 1 | 2 | 69 | 4 | 70 | 5.00e-21 | 79.0 |
MsaG017545 | MtrunA17_Chr8g0373121 | 54.412 | 68 | 30 | 1 | 2 | 69 | 4 | 70 | 5.00e-21 | 79.0 |
MsaG017545 | MtrunA17_Chr8g0373101 | 59.016 | 61 | 24 | 1 | 3 | 63 | 5 | 64 | 1.04e-20 | 78.2 |
MsaG017545 | MtrunA17_Chr5g0404101 | 43.750 | 80 | 41 | 1 | 1 | 76 | 17 | 96 | 1.05e-20 | 79.0 |
MsaG017545 | MtrunA17_Chr6g0449111 | 46.875 | 64 | 34 | 0 | 2 | 65 | 9 | 72 | 5.05e-18 | 71.6 |
MsaG017545 | MtrunA17_Chr5g0404301 | 53.226 | 62 | 28 | 1 | 2 | 63 | 3 | 63 | 5.57e-18 | 70.9 |
MsaG017545 | MtrunA17_Chr3g0081631 | 39.130 | 92 | 46 | 2 | 2 | 92 | 9 | 91 | 3.54e-17 | 69.7 |
MsaG017545 | MtrunA17_Chr1g0162081 | 40.299 | 67 | 40 | 0 | 3 | 69 | 12 | 78 | 2.23e-14 | 66.2 |
MsaG017545 | MtrunA17_Chr5g0436501 | 40.299 | 67 | 40 | 0 | 3 | 69 | 27 | 93 | 8.18e-14 | 64.3 |
MsaG017545 | MtrunA17_Chr8g0365011 | 42.857 | 63 | 36 | 0 | 3 | 65 | 8 | 70 | 2.93e-13 | 63.2 |
MsaG017545 | MtrunA17_Chr8g0364741 | 39.683 | 63 | 38 | 0 | 3 | 65 | 10 | 72 | 6.33e-13 | 59.7 |
MsaG017545 | MtrunA17_Chr5g0429521 | 44.444 | 63 | 35 | 0 | 3 | 65 | 29 | 91 | 1.98e-12 | 60.8 |
MsaG017545 | MtrunA17_Chr7g0241761 | 46.032 | 63 | 34 | 0 | 3 | 65 | 35 | 97 | 2.65e-12 | 60.5 |
MsaG017545 | MtrunA17_Chr3g0104071 | 44.444 | 63 | 35 | 0 | 3 | 65 | 43 | 105 | 4.94e-12 | 60.1 |
MsaG017545 | MtrunA17_Chr1g0210471 | 38.028 | 71 | 42 | 2 | 3 | 72 | 24 | 93 | 1.70e-11 | 57.8 |
MsaG017545 | MtrunA17_Chr1g0194071 | 53.571 | 56 | 25 | 1 | 2 | 56 | 10 | 65 | 3.49e-11 | 54.3 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG017545 | AT4G39250.1 | 47.778 | 90 | 44 | 2 | 3 | 92 | 14 | 100 | 7.86e-26 | 92.4 |
MsaG017545 | AT1G75250.2 | 48.889 | 90 | 41 | 2 | 3 | 91 | 12 | 97 | 3.57e-25 | 90.5 |
MsaG017545 | AT1G75250.1 | 57.812 | 64 | 27 | 0 | 3 | 66 | 12 | 75 | 5.97e-23 | 85.9 |
MsaG017545 | AT1G19510.1 | 59.016 | 61 | 25 | 0 | 6 | 66 | 15 | 75 | 1.26e-21 | 81.6 |
MsaG017545 | AT2G21650.1 | 43.333 | 90 | 49 | 2 | 3 | 92 | 14 | 101 | 4.07e-21 | 80.5 |
MsaG017545 | AT5G05790.2 | 48.485 | 66 | 34 | 0 | 2 | 67 | 30 | 95 | 1.11e-16 | 72.8 |
MsaG017545 | AT5G05790.1 | 48.485 | 66 | 34 | 0 | 2 | 67 | 30 | 95 | 1.11e-16 | 72.8 |
MsaG017545 | AT2G18328.1 | 51.667 | 60 | 29 | 0 | 7 | 66 | 15 | 74 | 5.88e-16 | 66.6 |
MsaG017545 | AT3G11280.2 | 41.176 | 68 | 40 | 0 | 3 | 70 | 33 | 100 | 3.87e-15 | 68.6 |
MsaG017545 | AT3G11280.1 | 41.176 | 68 | 40 | 0 | 3 | 70 | 33 | 100 | 3.87e-15 | 68.6 |
MsaG017545 | AT5G08520.1 | 40.244 | 82 | 49 | 0 | 3 | 84 | 12 | 93 | 6.32e-15 | 68.2 |
MsaG017545 | AT2G38090.1 | 46.032 | 63 | 34 | 0 | 3 | 65 | 29 | 91 | 1.03e-13 | 64.7 |
MsaG017545 | AT4G36570.1 | 61.364 | 44 | 17 | 0 | 3 | 46 | 12 | 55 | 1.21e-13 | 60.1 |
MsaG017545 | AT5G58900.1 | 46.032 | 63 | 34 | 0 | 3 | 65 | 34 | 96 | 1.32e-12 | 61.6 |
MsaG017545 | AT1G49010.1 | 38.667 | 75 | 43 | 1 | 3 | 74 | 8 | 82 | 1.86e-12 | 61.6 |
MsaG017545 | AT5G01200.1 | 39.506 | 81 | 48 | 1 | 3 | 82 | 30 | 110 | 8.08e-12 | 59.3 |
MsaG017545 | AT5G04760.1 | 39.683 | 63 | 37 | 1 | 3 | 65 | 6 | 67 | 8.74e-11 | 56.2 |
Find 24 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
ATCAACAATGGAAGGCAATT+TGG | 0.250643 | 3:-110535963 | MsaT017545.1:CDS |
TGCCACCGATTTGGATCTTC+TGG | 0.260812 | 3:+110536122 | None:intergenic |
AAATCCCACTGCCAACTTAC+AGG | 0.308746 | 3:-110536004 | MsaT017545.1:CDS |
GCCACATGTTGCCACCGATT+TGG | 0.310871 | 3:+110536113 | None:intergenic |
TTCGAATGAGATCTTCCTTT+AGG | 0.339675 | 3:+110536039 | None:intergenic |
GAGGATGCACTTGCAGTATA+TGG | 0.363746 | 3:-110536146 | MsaT017545.1:CDS |
GACAAATTAGCAGCATGGGT+TGG | 0.508959 | 3:-110536193 | None:intergenic |
AATCCCACTGCCAACTTACA+GGG | 0.517510 | 3:-110536003 | MsaT017545.1:CDS |
CCAACTTACAGGGGAGGTGC+TGG | 0.534339 | 3:-110535993 | MsaT017545.1:CDS |
CTCCCCTGTAAGTTGGCAGT+GGG | 0.537457 | 3:+110536000 | None:intergenic |
CCAGCACCTCCCCTGTAAGT+TGG | 0.540006 | 3:+110535993 | None:intergenic |
CAACATGTGGCTAATGCTGT+TGG | 0.541304 | 3:-110536101 | MsaT017545.1:CDS |
GGCAATTTGGCAATGAGCAA+AGG | 0.543201 | 3:-110535950 | MsaT017545.1:intron |
CCTCCCCTGTAAGTTGGCAG+TGG | 0.556127 | 3:+110535999 | None:intergenic |
ATGGTCCAGAAGATCCAAAT+CGG | 0.560223 | 3:-110536127 | MsaT017545.1:CDS |
CATGTGGCTAATGCTGTTGG+TGG | 0.579036 | 3:-110536098 | MsaT017545.1:CDS |
TCCAAATCGGTGGCAACATG+TGG | 0.582584 | 3:-110536114 | MsaT017545.1:CDS |
AATGCATTACGAGATCCTAA+AGG | 0.590523 | 3:-110536054 | MsaT017545.1:CDS |
GCTGGTATCAATATCAACAA+TGG | 0.592424 | 3:-110535975 | MsaT017545.1:CDS |
GTATCAATATCAACAATGGA+AGG | 0.611037 | 3:-110535971 | MsaT017545.1:CDS |
TGGTGGAAAATCCATACAAG+AGG | 0.660309 | 3:-110536081 | MsaT017545.1:CDS |
ATCCCACTGCCAACTTACAG+GGG | 0.669431 | 3:-110536002 | MsaT017545.1:CDS |
CCACTGCCAACTTACAGGGG+AGG | 0.671704 | 3:-110535999 | MsaT017545.1:CDS |
GTCCAGAAGATCCAAATCGG+TGG | 0.678303 | 3:-110536124 | MsaT017545.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr3 | gene | 110534819 | 110536201 | 110534819 | ID=MsaG017545 |
Chr3 | mRNA | 110534819 | 110536201 | 110534819 | ID=MsaT017545.1;Parent=MsaG017545 |
Chr3 | exon | 110534819 | 110534846 | 110534819 | ID=MsaT017545.1.exon2;Parent=MsaT017545.1 |
Chr3 | CDS | 110534819 | 110534846 | 110534819 | ID=cds.MsaT017545.1;Parent=MsaT017545.1 |
Chr3 | exon | 110535951 | 110536201 | 110535951 | ID=MsaT017545.1.exon1;Parent=MsaT017545.1 |
Chr3 | CDS | 110535951 | 110536201 | 110535951 | ID=cds.MsaT017545.1;Parent=MsaT017545.1 |
Gene Sequence |
Protein sequence |