AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017626


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017626 MtrunA17_Chr3g0142581 91.908 346 14 2 4 342 30 368 0.0 618
MsaG017626 MtrunA17_Chr1g0177351 43.169 366 170 11 2 342 67 419 3.03e-77 244
MsaG017626 MtrunA17_Chr4g0025881 41.925 322 156 6 45 342 100 414 8.97e-73 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017626 AT1G47870.1 48.366 306 112 9 46 342 123 391 1.69e-80 251
MsaG017626 AT1G47870.2 48.366 306 112 9 46 342 122 390 1.75e-80 251
MsaG017626 AT5G22220.2 41.543 337 149 7 45 342 92 419 3.04e-71 229
MsaG017626 AT5G22220.3 41.543 337 149 7 45 342 92 419 3.76e-71 229
MsaG017626 AT2G36010.3 39.193 347 173 8 26 342 112 450 1.29e-64 212
MsaG017626 AT2G36010.1 39.420 345 173 8 26 342 112 448 1.50e-64 212
MsaG017626 AT2G36010.2 36.152 343 168 9 26 342 162 479 1.79e-51 178

Find 86 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCAAACTGATAAATTTCT+TGG 0.195269 3:+111362748 None:intergenic
TACCATTATGCTTTCCTCTT+TGG 0.206217 3:+111363066 None:intergenic
ACAAATTACTCCTACGGATT+TGG 0.244715 3:-111360495 MsaT017626.1:CDS
TCGCTTGACTCAGAGGCTTT+TGG 0.259553 3:-111360524 MsaT017626.1:CDS
TAAAACTGCTGAGATCTTAA+AGG 0.260105 3:-111362693 MsaT017626.1:intron
ATATTTACTTCCACCGTATA+TGG 0.272766 3:+111362449 None:intergenic
TGCAGGTTGATGGTGATTAT+TGG 0.282905 3:-111360372 MsaT017626.1:intron
TCTTACCCTTCCATGCTTCC+AGG 0.295518 3:-111361135 MsaT017626.1:intron
CACTCAAATGAGCAGGAATT+AGG 0.313374 3:-111360902 MsaT017626.1:intron
ACAAATGTTCTTGAAGGAAT+AGG 0.317166 3:-111362496 MsaT017626.1:CDS
ATCTTCTAACTTTGCCTGCT+TGG 0.327328 3:+111360637 None:intergenic
ACAAACTTGACCAGTGTATA+AGG 0.337642 3:-111361402 MsaT017626.1:intron
ACCTCAATGAAACTAGCTTT+TGG 0.340106 3:+111361002 None:intergenic
AAGGGTAAGAATATCTTCTT+TGG 0.354876 3:+111361148 None:intergenic
TTCAAGAGGTACAGATTTAT+CGG 0.359261 3:+111360833 None:intergenic
TTATGCTTTCCTCTTTGGTT+GGG 0.365134 3:+111363071 None:intergenic
CAATCATTCTGTACTGCCTT+TGG 0.379050 3:+111360873 None:intergenic
GACATTACAAATGTTCTTGA+AGG 0.381084 3:-111362502 MsaT017626.1:CDS
ATTATGCTTTCCTCTTTGGT+TGG 0.394432 3:+111363070 None:intergenic
ACAATTCTGTTAGGCCAACT+TGG 0.403992 3:+111360335 None:intergenic
TAACTTGATGCTCCAGTTCG+TGG 0.414132 3:+111362318 None:intergenic
AAACTTCAAAGAACCATATA+CGG 0.421244 3:-111362462 MsaT017626.1:CDS
ACCTGTATTTGAAATCTGAT+TGG 0.422930 3:+111363013 None:intergenic
GAGGTGGGATTCAACTTATA+TGG 0.429057 3:-111360967 MsaT017626.1:CDS
AAGTTGAATCCCACCTCATC+AGG 0.430971 3:+111360973 None:intergenic
ATGATTGTTAGAAGTGCAAC+TGG 0.445965 3:-111360857 MsaT017626.1:CDS
GTCAATTCTCAGTGAAAGAA+AGG 0.446192 3:-111361309 MsaT017626.1:intron
AACCAAAGAGGAAAGCATAA+TGG 0.451388 3:-111363068 MsaT017626.1:CDS
GCCAATCAGATTTCAAATAC+AGG 0.451634 3:-111363014 MsaT017626.1:intron
CGGCTGCAGAATGGAGGGTG+TGG 0.459695 3:-111360597 MsaT017626.1:CDS
AATCGTTATGACAGTTCCTT+AGG 0.462492 3:-111362868 MsaT017626.1:intron
CAATTCTGTTAGGCCAACTT+GGG 0.473073 3:+111360336 None:intergenic
GCATCAAGTTAAATCTCTAA+AGG 0.477562 3:-111362306 MsaT017626.1:intron
GATGGACTTGAGCCACGAAC+TGG 0.489216 3:-111362330 MsaT017626.1:intron
TTGGGTTCAGTTTGAAGAAG+TGG 0.490056 3:+111363089 None:intergenic
CAGTTTGATCAATGAGGCTA+AGG 0.500273 3:-111362735 MsaT017626.1:CDS
AGTTGGCCTAACAGAATTGT+GGG 0.505242 3:-111360332 MsaT017626.1:CDS
TAAGCAACTTATTACAATCA+AGG 0.507030 3:-111361027 MsaT017626.1:CDS
TGAGGGTGTGAGTGCCAAGC+AGG 0.512509 3:-111360651 MsaT017626.1:CDS
CTGGTCAAGTTTGTATTCCT+CGG 0.512943 3:+111361411 None:intergenic
TCATTCTGTACTGCCTTTGG+TGG 0.517033 3:+111360876 None:intergenic
CAAGCACGACCACAAGTTTG+AGG 0.521197 3:-111360669 MsaT017626.1:CDS
AATTCAACAAATTACTCCTA+CGG 0.521739 3:-111360501 MsaT017626.1:CDS
AAGTTGGCCTAACAGAATTG+TGG 0.522443 3:-111360333 MsaT017626.1:CDS
AATCCCACCTCATCAGGATC+GGG 0.527403 3:+111360979 None:intergenic
AAAGGACCTGGAAGCATGGA+AGG 0.527585 3:+111361129 None:intergenic
CAGCCGTTGCTACTCCGTGA+TGG 0.529933 3:+111360614 None:intergenic
GAGTAGCAACGGCTGCAGAA+TGG 0.537502 3:-111360606 MsaT017626.1:CDS
AGGAATTAGGCTTCCACCAA+AGG 0.540877 3:-111360889 MsaT017626.1:CDS
AAGCACGACCACAAGTTTGA+GGG 0.544962 3:-111360668 MsaT017626.1:CDS
TTTCAATCAGATCCCCAAGT+TGG 0.557691 3:-111360349 MsaT017626.1:CDS
GGCTGCAGAATGGAGGGTGT+GGG 0.561995 3:-111360596 MsaT017626.1:CDS
ATTTATCAGTTTGATCAATG+AGG 0.562030 3:-111362741 MsaT017626.1:CDS
TCCAAAAGCTAGTTTCATTG+AGG 0.562628 3:-111361003 MsaT017626.1:CDS
GAATCCCACCTCATCAGGAT+CGG 0.563397 3:+111360978 None:intergenic
TTAACAGTGTTGCTCTGACT+CGG 0.564080 3:+111363119 None:intergenic
AAAGGAGCTTATAAGAAACC+TGG 0.565536 3:-111361291 MsaT017626.1:CDS
ACCTAGCTAACCTTATACAC+TGG 0.570136 3:+111361392 None:intergenic
AGGTCCCGATCCTGATGAGG+TGG 0.570705 3:-111360983 MsaT017626.1:CDS
GGTTCAGTTTGAAGAAGTGG+AGG 0.575422 3:+111363092 None:intergenic
CAGAAGAGAAGTACTTCAAG+AGG 0.575816 3:+111360819 None:intergenic
TAGCAACGGCTGCAGAATGG+AGG 0.582567 3:-111360603 MsaT017626.1:CDS
CACTCACACCCTCAAACTTG+TGG 0.587462 3:+111360660 None:intergenic
GATCCATCACGGAGTAGCAA+CGG 0.589664 3:-111360617 MsaT017626.1:CDS
TTTAAGTTCGCTTGACTCAG+AGG 0.590229 3:-111360531 MsaT017626.1:CDS
CAAACTGAACCCAACCAAAG+AGG 0.600334 3:-111363080 MsaT017626.1:CDS
AGCGAACTTAAACTCCCAGA+AGG 0.601180 3:+111360542 None:intergenic
CTTATAAGAAACCTGGAGGA+AGG 0.602931 3:-111361284 MsaT017626.1:CDS
TTGATCAATGAGGCTAAGGA+TGG 0.604623 3:-111362731 MsaT017626.1:CDS
GTGGGATTATTAGAAAACCA+AGG 0.618758 3:-111360578 MsaT017626.1:CDS
AGCAACGGCTGCAGAATGGA+GGG 0.619777 3:-111360602 MsaT017626.1:CDS
GCGAACTTAAACTCCCAGAA+GGG 0.628059 3:+111360543 None:intergenic
AATTCTGTTAGGCCAACTTG+GGG 0.629313 3:+111360337 None:intergenic
TTGAGGTCCCGATCCTGATG+AGG 0.629610 3:-111360986 MsaT017626.1:CDS
CTGGAGGAAGGTGAAAACAC+CGG 0.635878 3:-111361272 MsaT017626.1:CDS
ATTCATACATCCAAATCCGT+AGG 0.638390 3:+111360485 None:intergenic
TGGAGGAAGGTGAAAACACC+GGG 0.639040 3:-111361271 MsaT017626.1:CDS
GGTCCCGATCCTGATGAGGT+GGG 0.652728 3:-111360982 MsaT017626.1:CDS
AGTTGATAGCTTATATGCCG+AGG 0.657732 3:-111361428 MsaT017626.1:CDS
CAAAGTTAGAAGATCCATCA+CGG 0.671165 3:-111360628 MsaT017626.1:CDS
GTTGGCCTAACAGAATTGTG+GGG 0.672060 3:-111360331 MsaT017626.1:CDS
CTTCAAAGAACCATATACGG+TGG 0.675052 3:-111362459 MsaT017626.1:CDS
AAGGACCTGGAAGCATGGAA+GGG 0.676862 3:+111361130 None:intergenic
TTGGCCTAACAGAATTGTGG+GGG 0.685774 3:-111360330 MsaT017626.1:CDS
GGAGCTTATAAGAAACCTGG+AGG 0.707876 3:-111361288 MsaT017626.1:CDS
ACTGTCATAACGATTGACTG+TGG 0.771835 3:+111362876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 111360327 111363554 111360327 ID=MsaG017626
Chr3 mRNA 111360327 111363554 111360327 ID=MsaT017626.1;Parent=MsaG017626
Chr3 exon 111360327 111360389 111360327 ID=MsaT017626.1.exon13;Parent=MsaT017626.1
Chr3 CDS 111360327 111360389 111360327 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111360493 111360691 111360493 ID=MsaT017626.1.exon12;Parent=MsaT017626.1
Chr3 CDS 111360493 111360691 111360493 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111360833 111360909 111360833 ID=MsaT017626.1.exon11;Parent=MsaT017626.1
Chr3 CDS 111360833 111360909 111360833 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111360965 111361051 111360965 ID=MsaT017626.1.exon10;Parent=MsaT017626.1
Chr3 CDS 111360965 111361051 111360965 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111361136 111361184 111361136 ID=MsaT017626.1.exon9;Parent=MsaT017626.1
Chr3 CDS 111361136 111361184 111361136 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111361269 111361319 111361269 ID=MsaT017626.1.exon8;Parent=MsaT017626.1
Chr3 CDS 111361269 111361319 111361269 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111361403 111361455 111361403 ID=MsaT017626.1.exon7;Parent=MsaT017626.1
Chr3 CDS 111361403 111361455 111361403 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111362307 111362340 111362307 ID=MsaT017626.1.exon6;Parent=MsaT017626.1
Chr3 CDS 111362307 111362340 111362307 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111362457 111362545 111362457 ID=MsaT017626.1.exon5;Parent=MsaT017626.1
Chr3 CDS 111362457 111362545 111362457 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111362694 111362779 111362694 ID=MsaT017626.1.exon4;Parent=MsaT017626.1
Chr3 CDS 111362694 111362779 111362694 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111362869 111362913 111362869 ID=MsaT017626.1.exon3;Parent=MsaT017626.1
Chr3 CDS 111362869 111362913 111362869 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111363015 111363204 111363015 ID=MsaT017626.1.exon2;Parent=MsaT017626.1
Chr3 CDS 111363015 111363204 111363015 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Chr3 exon 111363546 111363554 111363546 ID=MsaT017626.1.exon1;Parent=MsaT017626.1
Chr3 CDS 111363546 111363554 111363546 ID=cds.MsaT017626.1;Parent=MsaT017626.1
Gene Sequence

>MsaT017626.1

ATGCCGCTTTTAAGTCAAAGAAATGAAATCAATAATGTCAACTTGCACACTAGTAGTAACGTTGAAGCGTTTCCGAGTCAGAGCAACACTGTTAATCTTCCTCCACTTCTTCAAACTGAACCCAACCAAAGAGGAAAGCATAATGGTAAATCAAAAGTTTCAAAAAATTCAAAATCTGCCAATCAGATTTCAAATACAGACTCTCTGAATTCAGCCACAGTCAATCGTTATGACAGTTCCTTAGGATTGTTAACCAAGAAATTTATCAGTTTGATCAATGAGGCTAAGGATGGTACCCTTGACCTAAATAAAACTGCTGAGATCTTAAAGGTTCAAAAAAGGAGAATTTATGACATTACAAATGTTCTTGAAGGAATAGGATTGATAGAGAAAACTTCAAAGAACCATATACGGTGGAACCACGAACTGGAGCATCAAGTTAAATCTCTAAAGGATGAAGTTGATAGCTTATATGCCGAGGAATACAAACTTGACCAGTGTATAAGTGAAAGAAAGGAGCTTATAAGAAACCTGGAGGAAGGTGAAAACACCGGGAAGTACCTTTTTTTTACCAAAGAAGATATTCTTACCCTTCCATGCTTCCAGAATAAGCAACTTATTACAATCAAGGCTCCAAAAGCTAGTTTCATTGAGGTCCCGATCCTGATGAGGTGGGATTCAACTTATATGGATGAATTAGGCTTCCACCAAAGGCAGTACAGAATGATTGTTAGAAGTGCAACTGGACCGATAAATCTGTACCTCTTGAACAAGCACGACCACAAGTTTGAGGGTGTGAGTGCCAAGCAGGCAAAGTTAGAAGATCCATCACGGAGTAGCAACGGCTGCAGAATGGAGGGTGTGGGATTATTAGAAAACCAAGGTTTTCAGAAAAACCCTTCTGGGAGTTTAAGTTCGCTTGACTCAGAGGCTTTTGGAATTCAACAAATTACTCCTACGGATTTGGATGTTGATGGTGATTATTGGTTTCAATCAGATCCCCAAGTTGGCCTAACAGAATTGTGGGGGTAG

Protein sequence

>MsaT017626.1

MPLLSQRNEINNVNLHTSSNVEAFPSQSNTVNLPPLLQTEPNQRGKHNGKSKVSKNSKSANQISNTDSLNSATVNRYDSSLGLLTKKFISLINEAKDGTLDLNKTAEILKVQKRRIYDITNVLEGIGLIEKTSKNHIRWNHELEHQVKSLKDEVDSLYAEEYKLDQCISERKELIRNLEEGENTGKYLFFTKEDILTLPCFQNKQLITIKAPKASFIEVPILMRWDSTYMDELGFHQRQYRMIVRSATGPINLYLLNKHDHKFEGVSAKQAKLEDPSRSSNGCRMEGVGLLENQGFQKNPSGSLSSLDSEAFGIQQITPTDLDVDGDYWFQSDPQVGLTELWG*