AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017644


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017644 MtrunA17_Chr3g0142871 97.706 654 14 1 1 654 1 653 0.0 1310
MsaG017644 MtrunA17_Chr7g0269901 97.095 654 18 1 1 654 1 653 0.0 1306
MsaG017644 MtrunA17_Chr3g0142891 83.712 264 35 2 393 654 1 258 5.20e-145 421
MsaG017644 MtrunA17_Chr8g0383991 29.439 214 95 3 443 653 140 300 8.01e-21 93.6
MsaG017644 MtrunA17_Chr4g0061421 30.417 240 115 9 98 300 9 233 2.30e-17 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017644 AT3G11450.1 63.939 660 210 11 4 654 7 647 0.0 734
MsaG017644 AT5G06110.1 59.492 669 239 10 2 651 5 660 0.0 713
MsaG017644 AT5G06110.2 59.492 669 239 10 2 651 5 660 0.0 713
MsaG017644 AT5G45420.2 31.959 194 87 3 459 652 158 306 7.81e-19 88.2
MsaG017644 AT5G45420.1 31.959 194 87 3 459 652 158 306 7.81e-19 88.2
MsaG017644 AT5G45420.3 31.959 194 87 3 459 652 158 306 7.81e-19 88.2
MsaG017644 AT1G74250.1 29.167 240 117 6 98 297 12 238 4.85e-16 82.4

Find 130 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTTCTTTGCCATATATT+TGG 0.137476 3:+111523133 None:intergenic
TTTCTCCTCTTCAGCAATTC+TGG 0.180978 3:+111523094 None:intergenic
GCAAGATCATTATGCATTAT+TGG 0.181634 3:-111523786 MsaT017644.1:CDS
TCCTTTCTTCTCAGTATTCT+AGG 0.194363 3:+111523191 None:intergenic
CCATGATGAAGGAAAATTTC+AGG 0.207011 3:-111522149 MsaT017644.1:CDS
CCATCTCCTTGAAGTTCCTT+TGG 0.238251 3:+111522641 None:intergenic
CTTTGGCTGTATCGGGCTTC+TGG 0.244533 3:+111522544 None:intergenic
CATCTCTGGTTAGCTTCCTT+TGG 0.256785 3:+111522231 None:intergenic
CAAGATCATTATGCATTATT+GGG 0.286954 3:-111523785 MsaT017644.1:CDS
GTCTAATTGTCTTCCGGTTA+AGG 0.294533 3:-111523996 MsaT017644.1:CDS
AGCTGAGAAGAAAAGGAAAA+AGG 0.327832 3:-111523159 MsaT017644.1:CDS
ATTGTCAACTAGAGAAGAAT+TGG 0.337451 3:-111522466 MsaT017644.1:CDS
TCATATTTCAACGCCTTAAC+CGG 0.338185 3:+111523983 None:intergenic
GAGAAAAGTAATCAACAAAA+TGG 0.344685 3:-111522777 MsaT017644.1:CDS
CATCTCCTTGAAGTTCCTTT+GGG 0.350239 3:+111522642 None:intergenic
CTGAATCCCGAGATCATAAA+AGG 0.353541 3:-111523310 MsaT017644.1:CDS
ATCTCTGGTTAGCTTCCTTT+GGG 0.356444 3:+111522232 None:intergenic
CGAGTAGCTGCAGCTGTACC+TGG 0.359607 3:-111522204 MsaT017644.1:CDS
TACTCACAAGAGCTTGTAGA+TGG 0.365954 3:-111524037 MsaT017644.1:CDS
AATGGCTATGTCAAGGCTAA+TGG 0.383665 3:-111522759 MsaT017644.1:CDS
TGTTTCGTCTAATTGTCTTC+CGG 0.396355 3:-111524002 MsaT017644.1:CDS
CTTCTGTTGCAAAGCAGCTT+CGG 0.407096 3:+111523040 None:intergenic
CAGCTACTCGTTCCCATCTC+TGG 0.408539 3:+111522217 None:intergenic
AGGCAAAAGCTGAGAAGAAA+AGG 0.413153 3:-111523166 MsaT017644.1:CDS
TTGTTGATAATGCTTATAAG+AGG 0.415278 3:-111523217 MsaT017644.1:CDS
AGCAAGAGCAAGAAGAAATC+TGG 0.416590 3:-111523827 MsaT017644.1:CDS
ATTCTTCTCTAGTTGACAAT+GGG 0.419152 3:+111522469 None:intergenic
TCATCACCTAGAGATGGAAT+TGG 0.419175 3:+111523434 None:intergenic
CCTTTCTTCTCAGTATTCTA+GGG 0.419637 3:+111523192 None:intergenic
GAAGAATGTGGTTGAAGATA+AGG 0.419917 3:-111523882 MsaT017644.1:CDS
CTGCTTGTTGCTGTCGGTAC+CGG 0.420172 3:+111522345 None:intergenic
TCAAAAGCTTTGGCTGTATC+GGG 0.425452 3:+111522537 None:intergenic
CAAGACTTCTACAAGGTGTT+TGG 0.427058 3:-111523497 MsaT017644.1:CDS
GCTGCTGCACTTAAACTTCT+CGG 0.428929 3:-111523938 MsaT017644.1:CDS
TGATGATAAGTCTTCAATAA+AGG 0.429949 3:-111523417 MsaT017644.1:CDS
AACTTCCTTGAGGTTTCATC+CGG 0.430850 3:-111523705 MsaT017644.1:CDS
TTTGGTATTCCTTTAAAAGC+TGG 0.431430 3:-111523373 MsaT017644.1:CDS
GAGGTTTCATCCGGACAAAC+AGG 0.431529 3:-111523696 MsaT017644.1:CDS
GAGATTGAGCTATTGAGAAA+AGG 0.438019 3:-111522678 MsaT017644.1:CDS
ATCAAAAGCTTTGGCTGTAT+CGG 0.439665 3:+111522536 None:intergenic
CAAATATATGGCAAAGAAGT+TGG 0.442593 3:-111523132 MsaT017644.1:CDS
AAGAAATGTATCAAAAGCTT+TGG 0.451091 3:+111522527 None:intergenic
ATTTGATATCGAACAATTGA+GGG 0.453929 3:-111522889 MsaT017644.1:CDS
CATTTGATATCGAACAATTG+AGG 0.454216 3:-111522890 MsaT017644.1:CDS
CCAAAGGAACTTCAAGGAGA+TGG 0.456430 3:-111522641 MsaT017644.1:CDS
CTTCGCAGCTTCAGTTTGCT+CGG 0.456856 3:+111523658 None:intergenic
CCAACACTGCATAAGCCTCT+TGG 0.462398 3:+111523594 None:intergenic
GATGCAATTGATTGTGCTGC+AGG 0.463598 3:+111522492 None:intergenic
ACAGCAGCAGCCTGTTTGTC+CGG 0.463811 3:+111523686 None:intergenic
CAAGATCAAACTCATCAGCC+TGG 0.467623 3:+111523339 None:intergenic
AGAGGCGTGGTCTGCAGTGC+AGG 0.469414 3:-111522286 MsaT017644.1:CDS
CTGGTTAGCTTCCTTTGGGA+AGG 0.471179 3:+111522236 None:intergenic
GTCGTCGTCATCGTCGGTGT+TGG 0.475726 3:+111522381 None:intergenic
CAAAGGAAGCTAACCAGAGA+TGG 0.482494 3:-111522230 MsaT017644.1:CDS
ATTGTTGTCGTCGTCATCGT+CGG 0.494599 3:+111522375 None:intergenic
TTGATACATTTCTTGAGAAG+AGG 0.495249 3:-111522518 MsaT017644.1:CDS
AGCTGCTTTGCAACAGAAGA+AGG 0.496514 3:-111523036 MsaT017644.1:CDS
CACTGGATCACGGTCTTCCC+AGG 0.497559 3:+111522186 None:intergenic
TCAATCTCTTCTTTAGTCCA+AGG 0.508908 3:+111522693 None:intergenic
AGCAGGCTACACAGAGAAGA+AGG 0.509420 3:-111522721 MsaT017644.1:CDS
CAGAGAAGAAGGAGAAACCT+TGG 0.509658 3:-111522710 MsaT017644.1:CDS
AAGATCAAACTCATCAGCCT+GGG 0.518267 3:+111523340 None:intergenic
AATTCTTCTCTAGTTGACAA+TGG 0.519138 3:+111522468 None:intergenic
CCCTAGAATACTGAGAAGAA+AGG 0.521673 3:-111523192 MsaT017644.1:CDS
CCGTAGCAAGATATCTAAGA+TGG 0.529700 3:+111523756 None:intergenic
CAAAGGAACTTCAAGGAGAT+GGG 0.529793 3:-111522640 MsaT017644.1:CDS
GTTGCTGTCGGTACCGGTGT+TGG 0.530623 3:+111522351 None:intergenic
GTTTCAGAATACATTGGCAC+TGG 0.531471 3:-111522612 MsaT017644.1:CDS
AATCAACCAATTCCATCTCT+AGG 0.533477 3:-111523440 MsaT017644.1:CDS
GCATGAAAAGAGTGACCAGC+CGG 0.535447 3:+111523959 None:intergenic
GGAAGCAGTAGTTCTCTAGC+AGG 0.540499 3:-111522738 MsaT017644.1:CDS
AGCGGCCAGAATTGCTGAAG+AGG 0.545593 3:-111523099 MsaT017644.1:CDS
ATCATAAAAGGTGGATGGAA+AGG 0.545814 3:-111523298 MsaT017644.1:CDS
TATGGCAAAGAAGTTGGAGG+AGG 0.545831 3:-111523126 MsaT017644.1:CDS
AGTTTCCCAAAGGAACTTCA+AGG 0.546208 3:-111522647 MsaT017644.1:CDS
CGAGCAAACTGAAGCTGCGA+AGG 0.546255 3:-111523657 MsaT017644.1:CDS
CCGAGATCATAAAAGGTGGA+TGG 0.548047 3:-111523303 MsaT017644.1:CDS
TTGATGCTGCTTGTTGCTGT+CGG 0.552607 3:+111522339 None:intergenic
CCAAGAGGCTTATGCAGTGT+TGG 0.555210 3:-111523594 MsaT017644.1:CDS
GCAAACTGAAGCTGCGAAGG+AGG 0.560752 3:-111523654 MsaT017644.1:CDS
AATACTGAGAAGAAAGGAAG+AGG 0.562192 3:-111523186 MsaT017644.1:CDS
CGTCCATTCCTCATAAAAGC+AGG 0.567375 3:+111523473 None:intergenic
TGGCTGTATCGGGCTTCTGG+AGG 0.570452 3:+111522547 None:intergenic
GACTTATCATCACCTAGAGA+TGG 0.570557 3:+111523428 None:intergenic
TATGAGGAATGGACGGTGGT+CGG 0.570845 3:-111523465 MsaT017644.1:CDS
CAACTAGAGAAGAATTGGAA+GGG 0.572911 3:-111522461 MsaT017644.1:CDS
AACACCTTGTAGAAGTCTTG+TGG 0.578361 3:+111523500 None:intergenic
ATCGGGCTTCTGGAGGAGAA+CGG 0.578804 3:+111522554 None:intergenic
TGTTGATAATGCTTATAAGA+GGG 0.580470 3:-111523216 MsaT017644.1:CDS
GAGGTTGTTTCAGAATACAT+TGG 0.584103 3:-111522618 MsaT017644.1:CDS
GGAGAAACAACGAAAAGCAG+AGG 0.585652 3:-111523078 MsaT017644.1:CDS
GAAGTTGGAGGAGGAAGAAG+CGG 0.588565 3:-111523117 MsaT017644.1:CDS
ATATATGGCAAAGAAGTTGG+AGG 0.590878 3:-111523129 MsaT017644.1:CDS
AAAGGAAGCTAACCAGAGAT+GGG 0.594916 3:-111522229 MsaT017644.1:CDS
AGCAACAAGCAGCATCAACT+CGG 0.597280 3:-111522334 MsaT017644.1:CDS
CCATCTTAGATATCTTGCTA+CGG 0.601973 3:-111523756 MsaT017644.1:CDS
AGCAAGAAGAAATCTGGTGA+CGG 0.602442 3:-111523821 MsaT017644.1:CDS
GAGTAGCTGCAGCTGTACCT+GGG 0.604010 3:-111522203 MsaT017644.1:CDS
AGTATGTGTTGTGACAAGAT+AGG 0.605889 3:+111522951 None:intergenic
TCAACAAAATGGCTATGTCA+AGG 0.605950 3:-111522766 MsaT017644.1:CDS
TCAACTAGAGAAGAATTGGA+AGG 0.608211 3:-111522462 MsaT017644.1:CDS
AACTAGAGAAGAATTGGAAG+GGG 0.608691 3:-111522460 MsaT017644.1:CDS
ACAGAAGAAGGTGAAAGAGA+AGG 0.608855 3:-111523024 MsaT017644.1:CDS
TCAAGGAGTTTCAGAACAAG+AGG 0.609387 3:-111522304 MsaT017644.1:CDS
GCTATCATAAAACTTCCTTG+AGG 0.611239 3:-111523715 MsaT017644.1:CDS
ACTCACAAGAGCTTGTAGAT+GGG 0.613229 3:-111524036 MsaT017644.1:CDS
CATGAAAAGAGTGACCAGCC+GGG 0.617908 3:+111523960 None:intergenic
CTTGTGAGTAAGTAATAAGA+CGG 0.621140 3:+111524050 None:intergenic
GTCTTGTGGATCACAGTCAG+TGG 0.627262 3:+111523514 None:intergenic
GGAATACAAAAGTTTCCCAA+AGG 0.628434 3:-111522657 MsaT017644.1:CDS
TCTTGTGGATCACAGTCAGT+GGG 0.629359 3:+111523515 None:intergenic
TAAGGCGTTGAAATATGACC+CGG 0.631520 3:-111523978 MsaT017644.1:CDS
ATCAACTCGGAAGACTCTCA+AGG 0.633952 3:-111522321 MsaT017644.1:CDS
AAGCTGGAGAGAGTTTCCCC+AGG 0.640812 3:-111523357 MsaT017644.1:CDS
TTTGTCCGGATGAAACCTCA+AGG 0.646808 3:+111523700 None:intergenic
TTTGATATCGAACAATTGAG+GGG 0.649974 3:-111522888 MsaT017644.1:CDS
GACGACAACAATTCCAACAC+CGG 0.650077 3:-111522364 MsaT017644.1:CDS
TCTTAGATATCTTGCTACGG+AGG 0.651757 3:-111523753 MsaT017644.1:CDS
TGATCCACAAGACTTCTACA+AGG 0.666734 3:-111523504 MsaT017644.1:CDS
AGATCAAACTCATCAGCCTG+GGG 0.670566 3:+111523341 None:intergenic
GGTTTGTGTGAGAAAATCGA+AGG 0.671097 3:-111522867 MsaT017644.1:CDS
GCGTTGAAATATGACCCGGC+TGG 0.672506 3:-111523974 MsaT017644.1:CDS
TGAGAAAATCGAAGGCAAAG+AGG 0.673494 3:-111522859 MsaT017644.1:CDS
AATCCCGAGATCATAAAAGG+TGG 0.674327 3:-111523307 MsaT017644.1:CDS
TTCTTCTCTAGTTGACAATG+GGG 0.676675 3:+111522470 None:intergenic
GAGTTTCAGAACAAGAGGCG+TGG 0.676797 3:-111522299 MsaT017644.1:CDS
TTGCTGCTGTAAGAATCAGA+AGG 0.678587 3:+111523845 None:intergenic
AAGCATTATCAACAAGAGTG+CGG 0.686922 3:+111523225 None:intergenic
AGGAACTTCAAGGAGATGGG+AGG 0.689319 3:-111522637 MsaT017644.1:CDS
AGATGCTGATAAGAAGAATG+TGG 0.708564 3:-111523894 MsaT017644.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 111522128 111524092 111522128 ID=MsaG017644
Chr3 mRNA 111522128 111524092 111522128 ID=MsaT017644.1;Parent=MsaG017644
Chr3 exon 111522128 111524092 111522128 ID=MsaT017644.1.exon1;Parent=MsaT017644.1
Chr3 CDS 111522128 111524092 111522128 ID=cds.MsaT017644.1;Parent=MsaT017644.1
Gene Sequence

>MsaT017644.1

ATGGCTGTTCAAACAAAATACCGTCTTATTACTTACTCACAAGAGCTTGTAGATGGGCAGCCTATTTTTGTTTCGTCTAATTGTCTTCCGGTTAAGGCGTTGAAATATGACCCGGCTGGTCACTCTTTTCATGCTGCTGCACTTAAACTTCTCGGTGTTACAAAAGAAGATAACAAAGATGCTGATAAGAAGAATGTGGTTGAAGATAAGGAACATGTTTATTTGCCTTCTGATTCTTACAGCAGCAAGAGCAAGAAGAAATCTGGTGACGGAGACAAGCAACAGCAAGATCATTATGCATTATTGGGTTTGAGCCATCTTAGATATCTTGCTACGGAGGATCAAATTCGTAAAAGCTATCATAAAACTTCCTTGAGGTTTCATCCGGACAAACAGGCTGCTGCTGTTCTTTCCGAGCAAACTGAAGCTGCGAAGGAGGCAAAGAAGATTGAAATAGATGTTCATTTTAAGGCAATCCAAGAGGCTTATGCAGTGTTGGTCGATCCCGTAAAAAGAAGAATTTATGATTCTACAGACGAGTTTGATGATGAGATTCCCACTGACTGTGATCCACAAGACTTCTACAAGGTGTTTGGTCCTGCTTTTATGAGGAATGGACGGTGGTCGGTTAATCAACCAATTCCATCTCTAGGTGATGATAAGTCTTCAATAAAGGAAGTTGATAGTTTCTACAATTTTTGGTATTCCTTTAAAAGCTGGAGAGAGTTTCCCCAGGCTGATGAGTTTGATCTTGAGCAAGCTGAATCCCGAGATCATAAAAGGTGGATGGAAAGGCAAAATGCAAAACTGTCAGAAAAAGCTAGGAAGGAAGAGTATGCACGTATCCGCACTCTTGTTGATAATGCTTATAAGAGGGACCCTAGAATACTGAGAAGAAAGGAAGAGGCAAAAGCTGAGAAGAAAAGGAAAAAGGAGTCCAAATATATGGCAAAGAAGTTGGAGGAGGAAGAAGCGGCCAGAATTGCTGAAGAGGAGAAACAACGAAAAGCAGAGGAAGATAAAAAAGCTGCCGAAGCTGCTTTGCAACAGAAGAAGGTGAAAGAGAAGGAGAAAAAGCTCTTGCGGAAGGAGCGAACACGTCTTCGAACTCTCTCAAGACCTATCTTGTCACAACACATACTTGATATTGCTGAAGACGATGTAGAAGAGCTTTGCATGTCATTTGATATCGAACAATTGAGGGGTTTGTGTGAGAAAATCGAAGGCAAAGAGGTGTTAGAGCAAGCAGAAGCTCTAAAAGATGCACTGAGTTGCAAGAAAAACGTGGTTGATGAGAAAAGTAATCAACAAAATGGCTATGTCAAGGCTAATGGAAGCAGTAGTTCTCTAGCAGGCTACACAGAGAAGAAGGAGAAACCTTGGACTAAAGAAGAGATTGAGCTATTGAGAAAAGGAATACAAAAGTTTCCCAAAGGAACTTCAAGGAGATGGGAGGTTGTTTCAGAATACATTGGCACTGGAAGATCTGTTGAAGAAATAATGAAAGCAACTAAAACCGTTCTCCTCCAGAAGCCCGATACAGCCAAAGCTTTTGATACATTTCTTGAGAAGAGGAAACCTGCAGCACAATCAATTGCATCCCCATTGTCAACTAGAGAAGAATTGGAAGGGGTCTCAATACCAGCAACAACAACTGAAAATAGTGATGCAAAAACAACAACTACTATACCAACACCGACGATGACGACGACAACAATTCCAACACCGGTACCGACAGCAACAAGCAGCATCAACTCGGAAGACTCTCAAGGAGTTTCAGAACAAGAGGCGTGGTCTGCAGTGCAGGAAAGAGCATTAGTTCAAGCTTTAAAAACCTTCCCAAAGGAAGCTAACCAGAGATGGGAACGAGTAGCTGCAGCTGTACCTGGGAAGACCGTGATCCAGTGCAAGAAAAAATTTGCCATGATGAAGGAAAATTTCAGGAACAAGAAAACTGCAGTTTGA

Protein sequence

>MsaT017644.1

MAVQTKYRLITYSQELVDGQPIFVSSNCLPVKALKYDPAGHSFHAAALKLLGVTKEDNKDADKKNVVEDKEHVYLPSDSYSSKSKKKSGDGDKQQQDHYALLGLSHLRYLATEDQIRKSYHKTSLRFHPDKQAAAVLSEQTEAAKEAKKIEIDVHFKAIQEAYAVLVDPVKRRIYDSTDEFDDEIPTDCDPQDFYKVFGPAFMRNGRWSVNQPIPSLGDDKSSIKEVDSFYNFWYSFKSWREFPQADEFDLEQAESRDHKRWMERQNAKLSEKARKEEYARIRTLVDNAYKRDPRILRRKEEAKAEKKRKKESKYMAKKLEEEEAARIAEEEKQRKAEEDKKAAEAALQQKKVKEKEKKLLRKERTRLRTLSRPILSQHILDIAEDDVEELCMSFDIEQLRGLCEKIEGKEVLEQAEALKDALSCKKNVVDEKSNQQNGYVKANGSSSSLAGYTEKKEKPWTKEEIELLRKGIQKFPKGTSRRWEVVSEYIGTGRSVEEIMKATKTVLLQKPDTAKAFDTFLEKRKPAAQSIASPLSTREELEGVSIPATTTENSDAKTTTTIPTPTMTTTTIPTPVPTATSSINSEDSQGVSEQEAWSAVQERALVQALKTFPKEANQRWERVAAAVPGKTVIQCKKKFAMMKENFRNKKTAV*