Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017687 | XP_024632959.1 | 52.424 | 330 | 80 | 6 | 1 | 253 | 1 | 330 | 6.62e-81 | 256 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017687 | sp|Q9SSW2|AZF2_ARATH | 40.206 | 97 | 39 | 4 | 45 | 137 | 106 | 187 | 1.49e-12 | 68.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017687 | tr|A0A396JGH2|A0A396JGH2_MEDTR | 52.424 | 330 | 80 | 6 | 1 | 253 | 1 | 330 | 3.16e-81 | 256 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG017687 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017687 | MtrunA17_Chr2g0331601 | 52.424 | 330 | 80 | 6 | 1 | 253 | 1 | 330 | 6.09e-85 | 256 |
| MsaG017687 | MtrunA17_Chr6g0481281 | 33.557 | 298 | 118 | 8 | 15 | 253 | 30 | 306 | 3.16e-32 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG017687 | AT3G19580.1 | 40.206 | 97 | 39 | 4 | 45 | 137 | 106 | 187 | 1.51e-13 | 68.9 |
| MsaG017687 | AT3G19580.2 | 40.206 | 97 | 39 | 4 | 45 | 137 | 106 | 187 | 1.51e-13 | 68.9 |
| MsaG017687 | AT1G49900.1 | 39.130 | 92 | 38 | 2 | 46 | 137 | 194 | 267 | 1.30e-11 | 64.7 |
| MsaG017687 | AT5G67450.1 | 39.175 | 97 | 48 | 2 | 45 | 137 | 97 | 186 | 3.60e-11 | 62.0 |
Find 36 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAATTTCTCTTTACCAAAA+TGG | 0.235457 | 3:-112117041 | MsaT017687.1:CDS |
| AAGCATGAGGTTATTATTAC+AGG | 0.279433 | 3:-112117459 | MsaT017687.1:CDS |
| TCTAACTTTATCTTCTTAAG+AGG | 0.301299 | 3:+112117360 | None:intergenic |
| TCCGACTCTTCATAAGTATT+CGG | 0.323788 | 3:+112117312 | None:intergenic |
| CCTCTTGTCAGACTCTTCAT+AGG | 0.349643 | 3:+112117527 | None:intergenic |
| CATAAAGAAGATCAAGCTTA+TGG | 0.366706 | 3:-112117063 | MsaT017687.1:CDS |
| TTACGAATAACATGATTTGC+TGG | 0.441171 | 3:+112117126 | None:intergenic |
| GAATGAGCCATATGCTATTG+AGG | 0.477019 | 3:-112116827 | MsaT017687.1:CDS |
| AGAAGAGGTAGAAAGTGTAT+TGG | 0.483023 | 3:-112117006 | MsaT017687.1:CDS |
| GCTTAGTCCTCAATAGCATA+TGG | 0.486464 | 3:+112116820 | None:intergenic |
| TTGGTGAGTCATCATCAAAT+TGG | 0.494071 | 3:-112116927 | MsaT017687.1:CDS |
| CTAACTTTATCTTCTTAAGA+GGG | 0.512429 | 3:+112117361 | None:intergenic |
| TGAGATCTCCGGTGAGGAAG+GGG | 0.514342 | 3:+112117335 | None:intergenic |
| ACTGATGATCAGCTAAAGAA+AGG | 0.524124 | 3:-112116868 | MsaT017687.1:CDS |
| GTGAGATCTCCGGTGAGGAA+GGG | 0.530993 | 3:+112117334 | None:intergenic |
| TAAGTATTCGGTGAGATCTC+CGG | 0.541580 | 3:+112117324 | None:intergenic |
| TTTAGCAGCGGACAAGCACT+TGG | 0.544373 | 3:-112117402 | MsaT017687.1:CDS |
| CTTATGAAGAGTCGGAAAAG+AGG | 0.546986 | 3:-112117305 | MsaT017687.1:CDS |
| GAAAAGCATTGGGTGGTCAC+CGG | 0.556634 | 3:-112117182 | MsaT017687.1:CDS |
| TCTTCTTTATGATGTTCAGT+AGG | 0.559242 | 3:+112117075 | None:intergenic |
| GGTGAGATCTCCGGTGAGGA+AGG | 0.559786 | 3:+112117333 | None:intergenic |
| GTTAGAAACCCCTTCCTCAC+CGG | 0.572034 | 3:-112117343 | MsaT017687.1:CDS |
| ACCGAATACTTATGAAGAGT+CGG | 0.572679 | 3:-112117313 | MsaT017687.1:CDS |
| TCTTAATAGAGTGAGATCTC+CGG | 0.581386 | 3:+112117163 | None:intergenic |
| GAGTCATCATCAAATTGGAA+TGG | 0.587360 | 3:-112116922 | MsaT017687.1:CDS |
| TGGCAAAAGAAAGATAGAAG+AGG | 0.598017 | 3:-112117021 | MsaT017687.1:CDS |
| AGCAGCGGACAAGCACTTGG+TGG | 0.609210 | 3:-112117399 | MsaT017687.1:CDS |
| TCTTGTTCACTATCAAGTGA+CGG | 0.609728 | 3:-112117492 | MsaT017687.1:CDS |
| CCTATGAAGAGTCTGACAAG+AGG | 0.641227 | 3:-112117527 | MsaT017687.1:CDS |
| CGAAGATGATGTCAAGCATG+AGG | 0.647694 | 3:-112117250 | MsaT017687.1:CDS |
| CGGAGATGATGCAAAGCATG+AGG | 0.655546 | 3:-112117472 | MsaT017687.1:CDS |
| GTGTATTGGTAGTGTTGAAG+CGG | 0.655661 | 3:-112116992 | MsaT017687.1:CDS |
| AAAAGTACTTATCTGAACGA+AGG | 0.659168 | 3:+112116953 | None:intergenic |
| TGGAATGGCAAGAATGAGCA+TGG | 0.683517 | 3:-112116907 | MsaT017687.1:CDS |
| ATTCGGTGAGATCTCCGGTG+AGG | 0.731513 | 3:+112117329 | None:intergenic |
| AATGGCAAGAATGAGCATGG+TGG | 0.807756 | 3:-112116904 | MsaT017687.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 112116822 | 112117583 | 112116822 | ID=MsaG017687 |
| Chr3 | mRNA | 112116822 | 112117583 | 112116822 | ID=MsaT017687.1;Parent=MsaG017687 |
| Chr3 | exon | 112116822 | 112117583 | 112116822 | ID=MsaT017687.1.exon1;Parent=MsaT017687.1 |
| Chr3 | CDS | 112116822 | 112117583 | 112116822 | ID=cds.MsaT017687.1;Parent=MsaT017687.1 |
| Gene Sequence |
| Protein sequence |
