AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017712

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017712 MtrunA17_Chr3g0144021 95.531 179 8 0 1 179 20 198 1.91e-129 362
MsaG017712 MtrunA17_Chr1g0212511 72.297 148 37 3 1 148 25 168 2.19e-72 219
MsaG017712 MtrunA17_Chr7g0222981 47.753 178 67 4 1 175 22 176 3.77e-45 147
MsaG017712 MtrunA17_Chr2g0283141 37.363 91 56 1 33 122 24 114 2.72e-15 73.2
MsaG017712 MtrunA17_Chr4g0043891 44.262 61 34 0 63 123 8 68 9.59e-14 67.4
MsaG017712 MtrunA17_Chr7g0223471 44.444 63 35 0 61 123 45 107 2.19e-13 67.4
MsaG017712 MtrunA17_Chr1g0153171 40.789 76 45 0 63 138 90 165 4.73e-13 65.5
MsaG017712 MtrunA17_Chr8g0392991 44.262 61 34 0 63 123 96 156 2.03e-12 64.7
MsaG017712 MtrunA17_Chr4g0031341 42.623 61 35 0 63 123 25 85 1.55e-11 61.2
MsaG017712 MtrunA17_Chr7g0238431 45.000 60 33 0 63 122 8 67 4.17e-11 59.7
MsaG017712 MtrunA17_Chr5g0436661 38.462 65 40 0 63 127 33 97 7.34e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017712 AT4G35550.1 54.774 199 74 3 1 184 38 235 7.73e-65 201
MsaG017712 AT1G20700.1 48.619 181 80 4 1 179 31 200 2.11e-55 174
MsaG017712 AT1G20700.3 48.352 182 81 4 1 180 31 201 2.40e-55 174
MsaG017712 AT1G20700.2 48.148 135 59 2 45 179 15 138 8.33e-41 135
MsaG017712 AT1G20710.1 46.409 181 87 5 1 179 15 187 4.35e-38 130
MsaG017712 AT5G45980.1 46.774 62 33 0 61 122 53 114 1.33e-14 70.9
MsaG017712 AT2G33880.2 45.161 62 34 0 61 122 53 114 2.70e-14 70.5
MsaG017712 AT2G33880.1 45.161 62 34 0 61 122 53 114 3.12e-14 70.1
MsaG017712 AT1G46480.2 47.541 61 32 0 63 123 90 150 1.01e-13 66.6
MsaG017712 AT1G46480.1 47.541 61 32 0 63 123 90 150 3.52e-13 66.2
MsaG017712 AT3G18010.1 40.845 71 42 0 63 133 76 146 4.38e-13 67.0
MsaG017712 AT3G11260.1 36.458 96 56 2 63 155 24 117 1.33e-12 63.5
MsaG017712 AT2G28610.1 46.667 60 32 0 63 122 8 67 4.37e-12 63.2
MsaG017712 AT5G05770.1 33.333 84 55 1 63 146 29 111 8.60e-12 60.1
MsaG017712 AT5G59340.1 43.077 65 37 0 59 123 10 74 1.56e-11 61.6
MsaG017712 AT2G01500.1 35.577 104 57 2 49 142 37 140 3.75e-11 60.8

Find 36 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGAGACTAATGTTTATAAT+TGG 0.232522 3:+112417013 MsaT017712.1:CDS
TTGAACGAGCTCTTCTGTTC+TGG 0.269547 3:-112417038 None:intergenic
TATGTCTTTGATCTTCTGTT+TGG 0.364760 3:-112416959 None:intergenic
TCAACTTCTGTGTCTGCCTC+TGG 0.402663 3:-112417102 None:intergenic
GTCTTTGATCTTCTGTTTGG+TGG 0.403902 3:-112416956 None:intergenic
CGTTGGAGTCCAACGCTGCC+TGG 0.416225 3:-112416881 None:intergenic
CTGGACACAAGATAACTTCC+AGG 0.432136 3:+112416863 MsaT017712.1:CDS
CTCAACAAGCTGATCAGAAA+TGG 0.436221 3:-112415485 None:intergenic
TGAGAGTTTATCACCTCATC+GGG 0.446050 3:+112417176 MsaT017712.1:CDS
CCACTGATGATGGCTTGCTC+TGG 0.448763 3:+112416844 MsaT017712.1:CDS
TCTTTGATCTTCTGTTTGGT+GGG 0.460764 3:-112416955 None:intergenic
TTGAGAGTTTATCACCTCAT+CGG 0.471970 3:+112417175 MsaT017712.1:CDS
AGTTATGACCGATGAACAAA+TGG 0.474281 3:+112415429 None:intergenic
ACCGTGTTGCCCTAGTTCAA+TGG 0.479896 3:-112416983 None:intergenic
TAAAAGTTCCATTTGTTCAT+CGG 0.487682 3:-112415437 None:intergenic
TTAAGCTTAATACCTATGTC+AGG 0.509678 3:-112417222 None:intergenic
TACGCTCAAGTATTTGAAGC+TGG 0.512249 3:-112416909 None:intergenic
ATTTGAAGCTGGATAGGCGT+TGG 0.517894 3:-112416898 None:intergenic
GTACTGTGATCCACTGATGA+TGG 0.540609 3:+112416834 MsaT017712.1:CDS
TCAAGTATTTGAAGCTGGAT+AGG 0.541162 3:-112416904 None:intergenic
AAGCAATCATCTGTTACTGC+AGG 0.542364 3:+112417066 MsaT017712.1:CDS
GAATGTTCAGGACTGAGACT+TGG 0.551998 3:+112416805 MsaT017712.1:intron
TCTATGAATACTCACTAACT+AGG 0.567909 3:+112417247 MsaT017712.1:CDS
ACCTGCGAGGTCGTGTTGAG+TGG 0.570461 3:-112415524 None:intergenic
AAAGACATAACCATTGAACT+AGG 0.570662 3:+112416973 MsaT017712.1:CDS
ACCACTCAACACGACCTCGC+AGG 0.579613 3:+112415523 MsaT017712.1:CDS
CCAATTATAAACATTAGTCT+CGG 0.584869 3:-112417013 None:intergenic
TTTATCACCTCATCGGGTCA+AGG 0.591799 3:+112417182 MsaT017712.1:CDS
AAGACATAACCATTGAACTA+GGG 0.603476 3:+112416974 MsaT017712.1:CDS
CCAGAGCAAGCCATCATCAG+TGG 0.606100 3:-112416844 None:intergenic
AGATAACTTCCAGGCAGCGT+TGG 0.612319 3:+112416872 MsaT017712.1:CDS
TACATATCCTTGACCCGATG+AGG 0.618531 3:-112417189 None:intergenic
AGGAAATCATGCAGAGCCAG+AGG 0.631315 3:+112417086 MsaT017712.1:CDS
ACCATTGAACTAGGGCAACA+CGG 0.634091 3:+112416982 MsaT017712.1:CDS
TACATTCAGAGTCCTGACAT+AGG 0.642781 3:+112417210 MsaT017712.1:CDS
ATAGTAGTAGTTTACCTGCG+AGG 0.718032 3:-112415537 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 112415433 112417272 112415433 ID=MsaG017712
Chr3 mRNA 112415433 112417272 112415433 ID=MsaT017712.1;Parent=MsaG017712
Chr3 exon 112415433 112415544 112415433 ID=MsaT017712.1.exon1;Parent=MsaT017712.1
Chr3 CDS 112415433 112415544 112415433 ID=cds.MsaT017712.1;Parent=MsaT017712.1
Chr3 exon 112416815 112417272 112416815 ID=MsaT017712.1.exon2;Parent=MsaT017712.1
Chr3 CDS 112416815 112417272 112416815 ID=cds.MsaT017712.1;Parent=MsaT017712.1
Gene Sequence

>MsaT017712.1

ATGACCGATGAACAAATGGAACTTTTAAGACAACAGATATCTGTTTACATCACCATTTCTGATCAGCTTGTTGAGATGCATAAATCTGTTACCACTCAACACGACCTCGCAGGACTGAGACTTGGTAATTTGTACTGTGATCCACTGATGATGGCTTGCTCTGGACACAAGATAACTTCCAGGCAGCGTTGGACTCCAACGCCTATCCAGCTTCAAATACTTGAGCGTATTTTCGATGAAGGCAATGGCACTCCCACCAAACAGAAGATCAAAGACATAACCATTGAACTAGGGCAACACGGTCAAATATCCGAGACTAATGTTTATAATTGGTTCCAGAACAGAAGAGCTCGTTCAAAGCGCAAGCAATCATCTGTTACTGCAGGAAATCATGCAGAGCCAGAGGCAGACACAGAAGTTGAGTCTCCAAAAGAGAACAAAACATGTGAAGAAAGCGATCTAGTTCAGTTCTTTGAGAGTTTATCACCTCATCGGGTCAAGGATATGTACATTCAGAGTCCTGACATAGGTATTAAGCTTAACTTCTATGAATACTCACTAACTAGGTAA
Protein sequence

>MsaT017712.1

MTDEQMELLRQQISVYITISDQLVEMHKSVTTQHDLAGLRLGNLYCDPLMMACSGHKITSRQRWTPTPIQLQILERIFDEGNGTPTKQKIKDITIELGQHGQISETNVYNWFQNRRARSKRKQSSVTAGNHAEPEADTEVESPKENKTCEESDLVQFFESLSPHRVKDMYIQSPDIGIKLNFYEYSLTR*