AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017743


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017743 MtrunA17_Chr3g0144341 94.226 381 10 3 1 378 1 372 0.0 731
MsaG017743 MtrunA17_Chr5g0407561 57.895 380 125 7 24 376 232 603 1.10e-140 413
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017743 AT1G76510.2 73.941 307 75 5 70 375 132 434 2.67e-154 442
MsaG017743 AT1G76510.4 73.941 307 75 5 70 375 132 434 2.67e-154 442
MsaG017743 AT1G76510.1 73.941 307 75 5 70 375 132 434 2.67e-154 442
MsaG017743 AT1G76510.3 69.231 325 72 6 75 375 137 457 3.63e-147 426
MsaG017743 AT1G20910.1 67.974 306 95 3 70 375 96 398 1.06e-140 406
MsaG017743 AT2G17410.1 64.238 302 101 3 75 375 489 784 5.54e-126 381
MsaG017743 AT2G17410.3 64.238 302 101 3 75 375 489 784 5.54e-126 381
MsaG017743 AT2G17410.2 64.238 302 95 3 75 375 489 778 5.50e-125 378
MsaG017743 AT1G20910.2 67.029 276 88 3 70 345 96 368 1.59e-120 354

Find 97 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGTATATGCTCTAGTTCC+TGG 0.233784 3:-112683327 MsaT017743.1:CDS
TTGGCATGCTCAGCGCCTTA+TGG 0.294765 3:-112684684 MsaT017743.1:CDS
GAAAAGCATAAGAGAGAGAT+TGG 0.315271 3:-112685756 MsaT017743.1:CDS
TTTGTAGACCGCTGTTTATC+AGG 0.316112 3:-112684759 MsaT017743.1:intron
CAATGGGACTTGATAAAACT+TGG 0.325300 3:+112687230 None:intergenic
GCAGCAGCAGCATCAGCAGC+AGG 0.327412 3:+112687557 None:intergenic
GTTGGACGACTTGTTATAAC+TGG 0.347669 3:-112682492 MsaT017743.1:CDS
TCTGTAACGTTCTTAACTTT+AGG 0.348861 3:+112687467 None:intergenic
GGGTATTACTCCCTTCAAAA+AGG 0.360882 3:-112682442 MsaT017743.1:intron
ACGTTCTTAACTTTAGGTTT+TGG 0.366210 3:+112687473 None:intergenic
TGGCATGCTCAGCGCCTTAT+GGG 0.389962 3:-112684683 MsaT017743.1:CDS
TTTCCACACTCGATGGTTGA+TGG 0.396113 3:+112685706 None:intergenic
AGGTAGGGCGCGGAGGGATG+CGG 0.401043 3:-112684726 MsaT017743.1:CDS
ATCAATGGAGGGTCATGAAA+TGG 0.404745 3:-112687633 None:intergenic
AACTAACAACAGCAGATGTT+TGG 0.411334 3:+112682179 None:intergenic
GGTTCCTGAGTCAGCTGTTA+AGG 0.416729 3:-112684651 MsaT017743.1:intron
ATGCTCGAGCTCTTTCATAA+AGG 0.418085 3:+112687287 None:intergenic
CTCCGCGCCCTACCTGAGCC+TGG 0.423147 3:+112684734 None:intergenic
CAGAAAATGCTGACAATCCA+TGG 0.427370 3:-112682464 MsaT017743.1:CDS
GTTTATCAGGCTCCAGGCTC+AGG 0.427487 3:-112684746 MsaT017743.1:CDS
AAAAGCATAAGAGAGAGATT+GGG 0.443286 3:-112685755 MsaT017743.1:CDS
TTGATCTTCACCCAATCAGC+TGG 0.444221 3:+112683495 None:intergenic
GACAGAGCTGCAAACATAGA+AGG 0.444672 3:-112684354 MsaT017743.1:CDS
GTTATAACAAGTCGTCCAAC+TGG 0.451498 3:+112682495 None:intergenic
TGATGAAGCCGGTACGCCTA+TGG 0.462210 3:-112687319 MsaT017743.1:CDS
AGGCTCAGGTAGGGCGCGGA+GGG 0.464479 3:-112684732 MsaT017743.1:CDS
CTGCACGTGCAATGCAAGGT+TGG 0.470880 3:-112684703 MsaT017743.1:CDS
TTTCTACAGATCGAGGTCCT+TGG 0.482284 3:-112687265 MsaT017743.1:CDS
AAGTATATGCTCTAGTTCCT+GGG 0.483026 3:-112683326 MsaT017743.1:CDS
TGTACGCGTTCCTTTCGAGC+AGG 0.485574 3:-112682140 MsaT017743.1:CDS
CTCCAGGCTCAGGTAGGGCG+CGG 0.493114 3:-112684736 MsaT017743.1:CDS
ACATACTTGAGGCAATTCAA+TGG 0.496631 3:+112687213 None:intergenic
GAACCTCGCCGTATCCCATA+AGG 0.497226 3:+112684669 None:intergenic
ATAAAGGCTTCTCGCTCCAT+AGG 0.502198 3:+112687303 None:intergenic
CAGGCTCAGGTAGGGCGCGG+AGG 0.503793 3:-112684733 MsaT017743.1:CDS
GAAGATCAATGTGCGGGAAA+CGG 0.506451 3:-112683481 MsaT017743.1:intron
TTGTTCAAGTTGAGGAGTGA+GGG 0.508455 3:+112687508 None:intergenic
ACAGAGAACACCGTCTGGTA+TGG 0.509878 3:-112684379 MsaT017743.1:CDS
ACCGCTGTTTATCAGGCTCC+AGG 0.511245 3:-112684752 MsaT017743.1:CDS
AAAGCATAAGAGAGAGATTG+GGG 0.512312 3:-112685754 MsaT017743.1:CDS
GAAGTCAATAATAACAATGC+TGG 0.518567 3:-112687446 MsaT017743.1:CDS
GGTTGATGGAGTGATCCGAC+AGG 0.527898 3:+112685720 None:intergenic
TCTGCTGTTGTTAGTTTGCA+TGG 0.528508 3:-112682172 MsaT017743.1:CDS
CATACTTGAGGCAATTCAAT+GGG 0.529182 3:+112687214 None:intergenic
TGATCTTCACCCAATCAGCT+GGG 0.529764 3:+112683496 None:intergenic
ACCTCTTCACGAAGGAGCCC+AGG 0.532624 3:+112683309 None:intergenic
CTGCAAACATAGAAGGAGAC+AGG 0.533192 3:-112684347 MsaT017743.1:CDS
TGTTGTTGTTGTTCAAGTTG+AGG 0.536049 3:+112687500 None:intergenic
TTCTCGCTCCATAGGCGTAC+CGG 0.546405 3:+112687311 None:intergenic
CACACAGCTCCCTGCTCGAA+AGG 0.552889 3:+112682130 None:intergenic
TCGAAGTTGTGGCGGTCAGT+AGG 0.553786 3:-112686414 MsaT017743.1:CDS
GTCGTCCAACTGGATCGGAT+TGG 0.556323 3:+112682505 None:intergenic
GCGGCTGCACGTGCAATGCA+AGG 0.557817 3:-112684707 MsaT017743.1:CDS
GCTCAGCGCCTTATGGGATA+CGG 0.558831 3:-112684677 MsaT017743.1:CDS
GGACTTGCACTACAGTCTCA+TGG 0.558888 3:-112686140 MsaT017743.1:CDS
AACAAACAGAGAACACCGTC+TGG 0.560624 3:-112684384 MsaT017743.1:CDS
AACAAGTCGTCCAACTGGAT+CGG 0.560628 3:+112682500 None:intergenic
TCCTGGGCTCCTTCGTGAAG+AGG 0.564218 3:-112683310 MsaT017743.1:intron
GTACGCGTTCCTTTCGAGCA+GGG 0.571183 3:-112682139 MsaT017743.1:CDS
AATGGGACTTGATAAAACTT+GGG 0.574691 3:+112687231 None:intergenic
GTTGTTCAAGTTGAGGAGTG+AGG 0.579571 3:+112687507 None:intergenic
AGAAAATGCTGACAATCCAT+GGG 0.582520 3:-112682463 MsaT017743.1:CDS
AGGTAACTGCATCGAAGTTG+TGG 0.585142 3:-112686425 MsaT017743.1:CDS
TAGGTTGGTCACAGCAGTAG+TGG 0.586143 3:-112683529 MsaT017743.1:CDS
TGGAGAGCTGTTATCAGACT+AGG 0.587815 3:-112686907 MsaT017743.1:CDS
TGGAGTGGATCAATTCTCGC+AGG 0.587913 3:+112682199 None:intergenic
TCAGGCTCCAGGCTCAGGTA+GGG 0.588503 3:-112684741 MsaT017743.1:CDS
ATATCTAGTCCTTACTTGGG+AGG 0.589671 3:+112686378 None:intergenic
CTTGGGAGGCTTGAATTCCA+AGG 0.591311 3:+112687248 None:intergenic
TTGAAGGGAGTAATACCCCA+TGG 0.594473 3:+112682447 None:intergenic
CTAGTCCTTACTTGGGAGGG+TGG 0.595303 3:+112686382 None:intergenic
CAATAATAACAATGCTGGCG+AGG 0.595839 3:-112687441 MsaT017743.1:intron
ATCAGGCTCCAGGCTCAGGT+AGG 0.598841 3:-112684742 MsaT017743.1:CDS
ATGGCTGCTTATGATGAAGC+CGG 0.600910 3:-112687330 MsaT017743.1:CDS
TATCTAGTCCTTACTTGGGA+GGG 0.608180 3:+112686379 None:intergenic
ACTCCATCAACCATCGAGTG+TGG 0.608784 3:-112685709 MsaT017743.1:CDS
GTCACAGCAGTAGTGGACAT+TGG 0.609262 3:-112683522 MsaT017743.1:CDS
CAGACCTTAACAGCTGACTC+AGG 0.614584 3:+112684647 None:intergenic
GGGACTTGATAAAACTTGGG+AGG 0.626061 3:+112687234 None:intergenic
GATCTTCACCCAATCAGCTG+GGG 0.626273 3:+112683497 None:intergenic
CTCAGACAGAAATCAATGGA+GGG 0.633845 3:-112687644 None:intergenic
CAGACTAGGTGGCTATGAAG+TGG 0.636788 3:-112686893 MsaT017743.1:intron
CGGGTCCAATCCGATCCAGT+TGG 0.638558 3:-112682510 MsaT017743.1:CDS
TTGGGTGAAGATCAATGTGC+GGG 0.643862 3:-112683487 MsaT017743.1:CDS
GGGGAGTTACAGCTCCCTGT+CGG 0.649300 3:-112685735 MsaT017743.1:CDS
ATCTTCACCCAATCAGCTGG+GGG 0.651763 3:+112683498 None:intergenic
TCGAGCATTTCTACAGATCG+AGG 0.659811 3:-112687272 MsaT017743.1:CDS
ACAACAGCAGATGTTTGGAG+TGG 0.672692 3:+112682184 None:intergenic
GCGCCTTATGGGATACGGCG+AGG 0.677937 3:-112684672 MsaT017743.1:CDS
GTTGATGGAGTGATCCGACA+GGG 0.680833 3:+112685721 None:intergenic
TAACTGCATCGAAGTTGTGG+CGG 0.684336 3:-112686422 MsaT017743.1:CDS
GCTCTGTCATCCATACCAGA+CGG 0.693775 3:+112684369 None:intergenic
GCCTGGAGCCTGATAAACAG+CGG 0.707020 3:+112684751 None:intergenic
ATTGGGTGAAGATCAATGTG+CGG 0.707083 3:-112683488 MsaT017743.1:CDS
AGAGCTGTTATCAGACTAGG+TGG 0.717567 3:-112686904 MsaT017743.1:CDS
CAGAGGTTACCTCTTCACGA+AGG 0.753208 3:+112683301 None:intergenic
GAAAATGCTGACAATCCATG+GGG 0.772709 3:-112682462 MsaT017743.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 112682129 112687651 112682129 ID=MsaG017743
Chr3 mRNA 112682129 112687651 112682129 ID=MsaT017743.1;Parent=MsaG017743
Chr3 exon 112682129 112682233 112682129 ID=MsaT017743.1.exon13;Parent=MsaT017743.1
Chr3 CDS 112682129 112682233 112682129 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112682443 112682535 112682443 ID=MsaT017743.1.exon12;Parent=MsaT017743.1
Chr3 CDS 112682443 112682535 112682443 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112683311 112683361 112683311 ID=MsaT017743.1.exon11;Parent=MsaT017743.1
Chr3 CDS 112683311 112683361 112683311 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112683482 112683548 112683482 ID=MsaT017743.1.exon10;Parent=MsaT017743.1
Chr3 CDS 112683482 112683548 112683482 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112684345 112684414 112684345 ID=MsaT017743.1.exon9;Parent=MsaT017743.1
Chr3 CDS 112684345 112684414 112684345 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112684492 112684543 112684492 ID=MsaT017743.1.exon8;Parent=MsaT017743.1
Chr3 CDS 112684492 112684543 112684492 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112684652 112684774 112684652 ID=MsaT017743.1.exon7;Parent=MsaT017743.1
Chr3 CDS 112684652 112684774 112684652 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112685701 112685793 112685701 ID=MsaT017743.1.exon6;Parent=MsaT017743.1
Chr3 CDS 112685701 112685793 112685701 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112686116 112686161 112686116 ID=MsaT017743.1.exon5;Parent=MsaT017743.1
Chr3 CDS 112686116 112686161 112686116 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112686393 112686445 112686393 ID=MsaT017743.1.exon4;Parent=MsaT017743.1
Chr3 CDS 112686393 112686445 112686393 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112686894 112686933 112686894 ID=MsaT017743.1.exon3;Parent=MsaT017743.1
Chr3 CDS 112686894 112686933 112686894 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112687219 112687352 112687219 ID=MsaT017743.1.exon2;Parent=MsaT017743.1
Chr3 CDS 112687219 112687352 112687219 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Chr3 exon 112687442 112687651 112687442 ID=MsaT017743.1.exon1;Parent=MsaT017743.1
Chr3 CDS 112687442 112687651 112687442 ID=cds.MsaT017743.1;Parent=MsaT017743.1
Gene Sequence

>MsaT017743.1

ATGGAGGGTCATGAAATGGATCTCGTTCAGAATGCTGCTGCTGCTGCTGCTGATCAACAAATGCTTGATCAACCTGCTGCTGATGCTGCTGCTGCTGCTGATGACACAAAAGTCAATTCTACCCTCACTCCTCAACTTGAACAACAACAACAACAACCAAAACCTAAAGTTAAGAACGTTACAGAAGTCAATAATAACAATGCTGGCGAGATGGCTGCTTATGATGAAGCCGGTACGCCTATGGAGCGAGAAGCCTTTATGAAAGAGCTCGAGCATTTCTACAGATCGAGGTCCTTGGAATTCAAGCCTCCCAAGTTTTATCAAGTCCCATTGAATTGCCTCAAGTTATGGAGAGCTGTTATCAGACTAGGTGGCTATGAAGTGGTAACTGCATCGAAGTTGTGGCGGTCAGTAGGAGAGTCTTTCCACCCTCCCAAGACTTGCACTACAGTCTCATGGACGTTTAGAATCTTTTATGAGAAGGCACTTTTAGAGTATGAAAAGCATAAGAGAGAGATTGGGGAGTTACAGCTCCCTGTCGGATCACTCCATCAACCATCGAGTGTGGAAAAAGAGACCGCTGTTTATCAGGCTCCAGGCTCAGGTAGGGCGCGGAGGGATGCGGCTGCACGTGCAATGCAAGGTTGGCATGCTCAGCGCCTTATGGGATACGGCGAGGTTCCTGAGTCAGCTGTTAAGGACAAGAACTTCAATCCCACACCAAAACGTGAAAAGAACCTAAAAAATATCGGTGTGATCAACAAACAGAGAACACCGTCTGGTATGGATGACAGAGCTGCAAACATAGAAGGAGACAGGCAGTTGGTCACAGCAGTAGTGGACATTGGACCCCCAGCTGATTGGGTGAAGATCAATGTGCGGGAAACGAAGGATTGTTTTGAAGTATATGCTCTAGTTCCTGGGCTCCTTCGTGAAGAGGTACGGGTCCAATCCGATCCAGTTGGACGACTTGTTATAACTGGTATGCCAGAAAATGCTGACAATCCATGGGGTATTACTCCCTTCAAAAAGGTTGTGAACTTACCTGCGAGAATTGATCCACTCCAAACATCTGCTGTTGTTAGTTTGCATGGTCGACTATTTGTACGCGTTCCTTTCGAGCAGGGAGCTGTGTGA

Protein sequence

>MsaT017743.1

MEGHEMDLVQNAAAAAADQQMLDQPAADAAAAADDTKVNSTLTPQLEQQQQQPKPKVKNVTEVNNNNAGEMAAYDEAGTPMEREAFMKELEHFYRSRSLEFKPPKFYQVPLNCLKLWRAVIRLGGYEVVTASKLWRSVGESFHPPKTCTTVSWTFRIFYEKALLEYEKHKREIGELQLPVGSLHQPSSVEKETAVYQAPGSGRARRDAAARAMQGWHAQRLMGYGEVPESAVKDKNFNPTPKREKNLKNIGVINKQRTPSGMDDRAANIEGDRQLVTAVVDIGPPADWVKINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITGMPENADNPWGITPFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFEQGAV*