AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017767

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017767 MtrunA17_Chr3g0144631 100.000 81 0 0 1 81 1 81 8.52e-57 169
MsaG017767 MtrunA17_Chr1g0155181 73.134 67 18 0 14 80 5 71 1.87e-34 112
MsaG017767 MtrunA17_Chr3g0141601 73.846 65 17 0 14 78 5 69 6.69e-32 106
MsaG017767 MtrunA17_Chr7g0255841 77.049 61 14 0 19 79 15 75 1.91e-30 102
MsaG017767 MtrunA17_Chr3g0081631 62.963 81 23 2 1 80 1 75 5.02e-29 99.4
MsaG017767 MtrunA17_Chr8g0373131 64.615 65 22 1 16 80 5 68 1.86e-27 94.7
MsaG017767 MtrunA17_Chr8g0373111 64.615 65 22 1 16 80 5 68 1.91e-27 94.7
MsaG017767 MtrunA17_Chr8g0373121 64.615 65 22 1 16 80 5 68 1.91e-27 94.7
MsaG017767 MtrunA17_Chr5g0404101 60.938 64 25 0 16 79 19 82 4.29e-26 92.0
MsaG017767 MtrunA17_Chr6g0449111 60.000 65 26 0 14 78 8 72 8.42e-26 90.9
MsaG017767 MtrunA17_Chr5g0404301 68.852 61 18 1 16 76 4 63 1.23e-25 89.7
MsaG017767 MtrunA17_Chr8g0373101 63.492 63 22 1 14 76 3 64 2.09e-25 89.4
MsaG017767 MtrunA17_Chr3g0141641 53.846 65 30 0 16 80 3 67 5.56e-22 81.6
MsaG017767 MtrunA17_Chr8g0364741 50.769 65 32 0 16 80 10 74 8.39e-21 79.3
MsaG017767 MtrunA17_Chr8g0365011 52.239 67 32 0 14 80 6 72 1.17e-20 82.4
MsaG017767 MtrunA17_Chr3g0104071 57.576 66 28 0 16 81 43 108 2.09e-20 82.4
MsaG017767 MtrunA17_Chr5g0429521 53.846 65 30 0 16 80 29 93 7.39e-20 80.9
MsaG017767 MtrunA17_Chr7g0241761 51.471 68 33 0 14 81 33 100 1.60e-18 77.0
MsaG017767 MtrunA17_Chr1g0162081 48.529 68 35 0 14 81 10 77 2.16e-18 76.6
MsaG017767 MtrunA17_Chr5g0436501 53.968 63 29 0 16 78 27 89 2.93e-18 75.9
MsaG017767 MtrunA17_Chr1g0194071 59.649 57 22 1 16 71 11 67 2.34e-16 67.4
MsaG017767 MtrunA17_Chr1g0210471 47.619 63 33 0 16 78 24 86 5.50e-16 69.7
MsaG017767 MtrunA17_Chr7g0270711 57.627 59 24 1 16 73 11 69 3.70e-15 63.9
MsaG017767 MtrunA17_Chr4g0062741 41.176 68 37 1 14 78 6 73 9.76e-14 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017767 AT1G75250.1 67.500 80 22 1 1 80 1 76 2.38e-35 116
MsaG017767 AT1G75250.2 67.500 80 22 1 1 80 1 76 2.98e-35 115
MsaG017767 AT4G39250.1 69.697 66 20 0 16 81 14 79 8.63e-34 112
MsaG017767 AT2G21650.1 66.667 66 22 0 16 81 14 79 1.88e-30 103
MsaG017767 AT1G19510.1 71.429 63 18 0 19 81 15 77 1.03e-29 101
MsaG017767 AT2G18328.1 69.841 63 19 0 19 81 14 76 7.59e-29 98.6
MsaG017767 AT4G36570.1 77.273 44 10 0 16 59 12 55 1.94e-21 79.3
MsaG017767 AT5G05790.2 52.941 68 32 0 14 81 29 96 2.78e-18 76.6
MsaG017767 AT5G05790.1 52.941 68 32 0 14 81 29 96 2.78e-18 76.6
MsaG017767 AT2G38090.1 53.846 65 30 0 16 80 29 93 1.45e-17 74.7
MsaG017767 AT3G11280.2 50.794 63 31 0 16 78 33 95 7.51e-17 72.4
MsaG017767 AT3G11280.1 50.794 63 31 0 16 78 33 95 7.51e-17 72.4
MsaG017767 AT5G58900.1 47.059 68 36 0 14 81 32 99 8.26e-17 72.8
MsaG017767 AT5G08520.1 49.254 67 34 0 14 80 10 76 3.31e-16 71.2
MsaG017767 AT5G04760.1 41.791 67 38 1 12 78 2 67 1.63e-13 62.8
MsaG017767 AT1G49010.1 42.647 68 36 1 14 78 6 73 6.37e-13 62.4
MsaG017767 AT5G01200.1 43.284 67 37 1 13 78 27 93 1.57e-12 60.8
MsaG017767 AT5G23650.1 39.189 74 45 0 5 78 2 75 1.21e-11 58.9

Find 16 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCACGTGGATTTGTAATT+TGG 0.099924 3:+113062008 None:intergenic
ACAAGCCTTGTGATTCAATT+TGG 0.187124 3:-113062208 MsaT017767.1:CDS
GTGGATTTGTAATTTGGAAT+AGG 0.364725 3:+113062014 None:intergenic
AGCACTTGCAAAATTTGATA+AGG 0.422428 3:-113062156 MsaT017767.1:CDS
CACAAGGCTTGTGTTTGCTA+AGG 0.474591 3:+113062219 None:intergenic
GATCTCAAACACATTGAGTC+TGG 0.498892 3:-113062044 MsaT017767.1:CDS
GAGACACTATGAGCTACTCT+TGG 0.504426 3:-113062069 MsaT017767.1:CDS
CTAATTTGCTACACTTCACG+TGG 0.522033 3:+113061995 None:intergenic
ACAAGGCTTGTGTTTGCTAA+GGG 0.544579 3:+113062220 None:intergenic
ACACTATGAGCTACTCTTGG+AGG 0.567606 3:-113062066 MsaT017767.1:CDS
GTGTTTGCTAAGGGAACTTG+AGG 0.586339 3:+113062229 None:intergenic
AATGTAGCAAAAGCAGTTGG+TGG 0.597235 3:-113062113 MsaT017767.1:CDS
CAAAATGTAGCAAAAGCAGT+TGG 0.599555 3:-113062116 MsaT017767.1:CDS
GGAGTCCAAATTGAATCACA+AGG 0.603739 3:+113062203 None:intergenic
ATAAGGATACCCCTGACCGT+TGG 0.605106 3:-113062139 MsaT017767.1:CDS
AGTTGGTGGTAAATCAGTTG+AGG 0.635333 3:-113062099 MsaT017767.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 113062010 113062255 113062010 ID=MsaG017767
Chr3 mRNA 113062010 113062255 113062010 ID=MsaT017767.1;Parent=MsaG017767
Chr3 exon 113062010 113062255 113062010 ID=MsaT017767.1.exon1;Parent=MsaT017767.1
Chr3 CDS 113062010 113062255 113062010 ID=cds.MsaT017767.1;Parent=MsaT017767.1
Gene Sequence

>MsaT017767.1

ATGGCCTCAAGTTCCCTTAGCAAACACAAGCCTTGTGATTCAATTTGGACTCCAAAACAAAACAAGTTGTTTGAAAAAGCACTTGCAAAATTTGATAAGGATACCCCTGACCGTTGGCAAAATGTAGCAAAAGCAGTTGGTGGTAAATCAGTTGAGGAAGTTAAGAGACACTATGAGCTACTCTTGGAGGATCTCAAACACATTGAGTCTGGTCATGTTCCTATTCCAAATTACAAATCCACGTGA
Protein sequence

>MsaT017767.1

MASSSLSKHKPCDSIWTPKQNKLFEKALAKFDKDTPDRWQNVAKAVGGKSVEEVKRHYELLLEDLKHIESGHVPIPNYKST*