AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017791

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017791 MtrunA17_Chr3g0144941 97.419 155 3 1 1 154 1 155 1.34e-106 300
MsaG017791 MtrunA17_Chr4g0035414 71.895 153 39 1 1 149 1 153 8.97e-65 194
MsaG017791 MtrunA17_Chr1g0155551 61.491 161 50 4 1 151 1 159 3.75e-57 176
MsaG017791 MtrunA17_Chr8g0378901 53.416 161 67 5 1 154 1 160 1.73e-46 148
MsaG017791 MtrunA17_Chr3g0141881 59.341 91 34 1 17 107 53 140 3.91e-30 107
MsaG017791 MtrunA17_Chr1g0194681 44.545 110 58 1 1 110 1 107 4.33e-25 94.0
MsaG017791 MtrunA17_Chr6g0456061 44.118 102 47 2 26 124 86 180 3.21e-18 77.4
MsaG017791 MtrunA17_Chr8g0341311 50.685 73 36 0 26 98 70 142 2.54e-17 74.7
MsaG017791 MtrunA17_Chr7g0225661 44.444 99 51 2 1 98 57 152 2.61e-17 74.7
MsaG017791 MtrunA17_Chr7g0220571 51.250 80 39 0 19 98 76 155 4.44e-17 74.3
MsaG017791 MtrunA17_Chr4g0054291 39.091 110 60 1 7 109 45 154 6.39e-16 70.9
MsaG017791 MtrunA17_Chr5g0400901 41.053 95 48 1 27 121 58 144 2.04e-14 66.6
MsaG017791 MtrunA17_Chr7g0269951 45.000 120 60 2 27 146 19 132 4.51e-13 62.4
MsaG017791 MtrunA17_Chr3g0139301 34.259 108 67 1 6 109 98 205 1.14e-12 63.9
MsaG017791 MtrunA17_Chr8g0369161 36.735 98 58 2 24 118 300 396 2.90e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017791 AT1G75390.1 53.459 159 58 4 2 147 9 164 2.83e-48 154
MsaG017791 AT4G34590.1 57.143 133 47 2 26 148 23 155 5.19e-44 142
MsaG017791 AT2G18160.1 47.020 151 56 5 22 151 23 170 7.83e-37 125
MsaG017791 AT1G75390.2 60.825 97 34 2 2 94 9 105 1.21e-32 112
MsaG017791 AT3G62420.1 44.526 137 75 1 13 149 8 143 1.29e-30 108
MsaG017791 AT2G04038.1 48.837 86 41 1 16 98 57 142 4.39e-18 76.6
MsaG017791 AT5G15830.1 34.932 146 79 3 9 154 55 184 8.82e-18 76.3
MsaG017791 AT1G13600.1 45.361 97 48 2 16 107 70 166 1.16e-16 73.6
MsaG017791 AT3G30530.1 52.055 73 35 0 26 98 77 149 2.79e-16 72.0
MsaG017791 AT5G38800.1 47.500 80 42 0 19 98 61 140 1.48e-15 70.1
MsaG017791 AT5G49450.1 36.190 105 64 1 27 128 13 117 1.96e-12 61.2
MsaG017791 AT4G37730.1 45.000 80 37 1 26 98 193 272 4.52e-12 62.8
MsaG017791 AT2G22850.1 37.079 89 56 0 23 111 122 210 2.78e-11 59.7
MsaG017791 AT2G22850.2 37.079 89 56 0 23 111 122 210 2.78e-11 59.7
MsaG017791 AT3G49760.1 55.814 43 19 0 28 70 70 112 4.41e-11 58.2
MsaG017791 AT1G68880.1 38.554 83 51 0 12 94 29 111 4.49e-11 57.8

Find 21 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCCTCTGAACCTGAATT+TGG 0.134136 3:-113326346 None:intergenic
CTTCAACAACCCTTTGAATA+TGG 0.147562 3:+113326690 MsaT017791.1:CDS
CCTCACAACTCAACGATTCT+TGG 0.288929 3:+113326528 MsaT017791.1:CDS
TGTTCAACACTTCCAAATTC+AGG 0.300497 3:+113326334 MsaT017791.1:CDS
CTTGGATGATCTTGCTGTTC+AGG 0.305122 3:+113326459 MsaT017791.1:CDS
TCTTCAAGTGGAACATCTTC+AGG 0.306987 3:+113326310 MsaT017791.1:CDS
TTCAACAACCCTTTGAATAT+GGG 0.356244 3:+113326691 MsaT017791.1:CDS
TTGTCATTATTATTATCAAC+AGG 0.406404 3:-113326664 None:intergenic
TTATTTGAACCAACCAATCA+TGG 0.433242 3:+113326714 MsaT017791.1:CDS
TTCAAATAACCCATATTCAA+AGG 0.450188 3:-113326700 None:intergenic
GAGATCTTCTTGCTGATTCG+CGG 0.468319 3:-113326417 None:intergenic
ATATCTGCAGAAGCCATGAT+TGG 0.469872 3:-113326727 None:intergenic
GGAAGATTTGATGCTTCTGA+TGG 0.470481 3:+113326363 MsaT017791.1:CDS
TCAAATAACCCATATTCAAA+GGG 0.472163 3:-113326699 None:intergenic
CTGCAGAAGCCATGATTGGT+TGG 0.535667 3:-113326723 None:intergenic
AACTTCATGAATGTTGCAAA+TGG 0.546215 3:+113326631 MsaT017791.1:CDS
CTGTTCAGGTGAGTCAGCTA+AGG 0.555920 3:+113326473 MsaT017791.1:CDS
TCTCACATGGCTTCTTCAAG+TGG 0.568523 3:+113326298 None:intergenic
ACTTCCAAATTCAGGTTCAG+AGG 0.572504 3:+113326342 MsaT017791.1:CDS
CAGCAAGAAGATCTCGAATG+AGG 0.587735 3:+113326425 MsaT017791.1:CDS
CCAAGAATCGTTGAGTTGTG+AGG 0.599678 3:-113326528 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 113326304 113326768 113326304 ID=MsaG017791
Chr3 mRNA 113326304 113326768 113326304 ID=MsaT017791.1;Parent=MsaG017791
Chr3 exon 113326304 113326768 113326304 ID=MsaT017791.1.exon1;Parent=MsaT017791.1
Chr3 CDS 113326304 113326768 113326304 ID=cds.MsaT017791.1;Parent=MsaT017791.1
Gene Sequence

>MsaT017791.1

ATGGCTTCTTCAAGTGGAACATCTTCAGGCTGTTCAACACTTCCAAATTCAGGTTCAGAGGAAGATTTGATGCTTCTGATGGATCAAAGAAAAAGAAAGAGAATGATATCAAACCGCGAATCAGCAAGAAGATCTCGAATGAGGAAACAAAAACACTTGGATGATCTTGCTGTTCAGGTGAGTCAGCTAAGGAATGAGAATCAACAGATTCTAACTTCAGTGAACCTCACAACTCAACGATTCTTGGCTGTTGAATCTGAAAACTCTGTGCTTAGAGCTCAACTGAATGAACTCAACAGCAGATTTGAGTCTTTGAATGAGATCATCAACTTCATGAATGTTGCAAATGGTGTTTTTGAACCTGTTGATAATAATAATGACAATTACTTCAACAACCCTTTGAATATGGGTTATTTGAACCAACCAATCATGGCTTCTGCAGATATGAACATGATACATTATTGA
Protein sequence

>MsaT017791.1

MASSSGTSSGCSTLPNSGSEEDLMLLMDQRKRKRMISNRESARRSRMRKQKHLDDLAVQVSQLRNENQQILTSVNLTTQRFLAVESENSVLRAQLNELNSRFESLNEIINFMNVANGVFEPVDNNNDNYFNNPLNMGYLNQPIMASADMNMIHY*