AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017800 MtrunA17_Chr3g0145041 91.209 182 16 0 1 182 15 196 2.96e-126 353
MsaG017800 MtrunA17_Chr3g0145031 71.505 186 49 1 1 182 33 218 1.13e-99 286
MsaG017800 MtrunA17_Chr2g0316511 54.839 186 80 2 1 182 31 216 1.11e-72 218
MsaG017800 MtrunA17_Chr2g0324381 51.923 156 70 2 8 158 44 199 1.85e-54 171
MsaG017800 MtrunA17_Chr5g0447451 44.361 133 64 4 34 156 11 143 3.65e-29 105
MsaG017800 MtrunA17_Chr2g0324361 48.387 93 42 3 1 90 18 107 1.59e-20 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017800 AT4G39100.1 59.563 183 69 2 1 179 31 212 1.07e-81 242
MsaG017800 AT4G22140.2 55.914 186 78 2 1 182 39 224 5.46e-78 232
MsaG017800 AT4G22140.1 55.307 179 76 2 1 175 39 217 1.44e-73 221
MsaG017800 AT4G22140.3 55.307 179 76 2 1 175 39 217 1.44e-73 221
MsaG017800 AT4G39100.2 63.910 133 44 1 1 129 31 163 1.66e-63 193
MsaG017800 AT4G04260.1 48.045 179 87 3 1 174 1 178 2.00e-56 176
MsaG017800 AT4G04260.2 48.045 179 87 3 1 174 14 191 3.48e-56 176
MsaG017800 AT4G04260.3 55.046 109 46 1 1 106 32 140 6.57e-38 127

Find 36 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGGAATACCTTTGTATT+TGG 0.149988 3:-113389290 None:intergenic
AATGAAGTTGTCAATTCTTT+CGG 0.316759 3:-113389204 None:intergenic
TTAGGAATACCTTTGTATTT+GGG 0.355673 3:-113389289 None:intergenic
ACCATCACTTTCATCTTTGA+CGG 0.371838 3:-113390137 None:intergenic
AAAATGCACTGTTCACAGTT+TGG 0.374580 3:+113387568 MsaT017800.1:CDS
CACTGGATCATAACTTCATC+AGG 0.392678 3:-113387878 None:intergenic
CATAACTTCATCAGGGTTGT+AGG 0.393557 3:-113387870 None:intergenic
ACTCACACTTGAACTATATC+AGG 0.399463 3:-113387674 None:intergenic
TGTTGGTATCCACGGTTTAG+AGG 0.405406 3:-113387381 None:intergenic
TTCGTGTCCGTTGGTACTAC+TGG 0.414838 3:+113387447 MsaT017800.1:CDS
TTGAACTACAATTCCGCTAC+TGG 0.456893 3:+113387641 MsaT017800.1:CDS
CAAGAGACGTTCGTGTCCGT+TGG 0.522823 3:+113387438 MsaT017800.1:CDS
CGACCCTTCTTTGTCTCCTC+CGG 0.527955 3:-113387470 None:intergenic
TACATGAAGCTTGATGTTGT+TGG 0.528797 3:+113387596 MsaT017800.1:CDS
AGTGTGATCGCTGCACTGAT+TGG 0.536011 3:+113387897 MsaT017800.1:CDS
GCCACATATGTTGGTATCCA+CGG 0.537762 3:-113387389 None:intergenic
ATAACTTCATCAGGGTTGTA+GGG 0.552648 3:-113387869 None:intergenic
ACCGTCATGTCTATACAAGC+AGG 0.569799 3:-113389173 None:intergenic
AAGGGTCGGCGTCATTTCCA+TGG 0.570085 3:+113387485 MsaT017800.1:CDS
CTGGCCGGAGGAGACAAAGA+AGG 0.572097 3:+113387466 MsaT017800.1:CDS
ACCGTGGATACCAACATATG+TGG 0.579459 3:+113387388 MsaT017800.1:CDS
TGTCCGTTGGTACTACTGGC+CGG 0.580901 3:+113387451 MsaT017800.1:CDS
TCGATCATAGCCACATATGT+TGG 0.588140 3:-113387398 None:intergenic
GCGTCATTTCCATGGCTCAA+AGG 0.593809 3:+113387493 MsaT017800.1:CDS
CGGAGGAGACAAAGAAGGGT+CGG 0.615656 3:+113387471 MsaT017800.1:CDS
TCCTGCTTGTATAGACATGA+CGG 0.620123 3:+113389172 MsaT017800.1:CDS
AATCAGTGCAGCGATCACAC+TGG 0.630754 3:-113387895 None:intergenic
TGGCCGGAGGAGACAAAGAA+GGG 0.641254 3:+113387467 MsaT017800.1:CDS
CAGAGTGTTGATACAATCGA+AGG 0.642499 3:+113387545 MsaT017800.1:CDS
AGGAGTGAGAGCACCAGTAG+CGG 0.642633 3:-113387654 None:intergenic
CCTCCGGCCAGTAGTACCAA+CGG 0.658998 3:-113387454 None:intergenic
TGCAAGACTCCCAAATACAA+AGG 0.662219 3:+113389280 MsaT017800.1:CDS
ACTGGATCATAACTTCATCA+GGG 0.673831 3:-113387877 None:intergenic
GCATGCGTTCCTCTAAACCG+TGG 0.713047 3:+113387372 None:intergenic
CCGTTGGTACTACTGGCCGG+AGG 0.722355 3:+113387454 MsaT017800.1:CDS
TGCTTGTATAGACATGACGG+TGG 0.750089 3:+113389175 MsaT017800.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 113387374 113390149 113387374 ID=MsaG017800
Chr3 mRNA 113387374 113390149 113387374 ID=MsaT017800.1;Parent=MsaG017800
Chr3 exon 113387374 113387690 113387374 ID=MsaT017800.1.exon1;Parent=MsaT017800.1
Chr3 CDS 113387374 113387690 113387374 ID=cds.MsaT017800.1;Parent=MsaT017800.1
Chr3 exon 113387850 113387918 113387850 ID=MsaT017800.1.exon2;Parent=MsaT017800.1
Chr3 CDS 113387850 113387918 113387850 ID=cds.MsaT017800.1;Parent=MsaT017800.1
Chr3 exon 113389166 113389301 113389166 ID=MsaT017800.1.exon3;Parent=MsaT017800.1
Chr3 CDS 113389166 113389301 113389166 ID=cds.MsaT017800.1;Parent=MsaT017800.1
Chr3 exon 113390123 113390149 113390123 ID=MsaT017800.1.exon4;Parent=MsaT017800.1
Chr3 CDS 113390123 113390149 113390123 ID=cds.MsaT017800.1;Parent=MsaT017800.1
Gene Sequence

>MsaT017800.1

ATGCGTTCCTCTAAACCGTGGATACCAACATATGTGGCTATGATCGAAGAGATGAAAGCTGATTCAAGAGACGTTCGTGTCCGTTGGTACTACTGGCCGGAGGAGACAAAGAAGGGTCGGCGTCATTTCCATGGCTCAAAGGAGCTTTTTCTATCTGATCATTTAGATGTTCAGAGTGTTGATACAATCGAAGGAAAATGCACTGTTCACAGTTTGGAGAACTACATGAAGCTTGATGTTGTTGGTGATGATGATTTCTTCTGTCGATTGAACTACAATTCCGCTACTGGTGCTCTCACTCCTGATATAGTTCAAGTCTATTGTAAATGTGAGATGCCCTACAACCCTGATGAAGTTATGATCCAGTGTGATCGCTGCACTGATTGGTTTCATCCTGCTTGTATAGACATGACGGTGGAAGAAGCCGAAAGAATTGACAACTTCATTTGTGAAAGTTGTTCTGCCGAAGTTCAAAAAAAGTTGCAAGATTCTCATTCTGCTGCAAGACTCCCAAATACAAAGGTGGATACTAAACGCCGTCAAAGATGA

Protein sequence

>MsaT017800.1

MRSSKPWIPTYVAMIEEMKADSRDVRVRWYYWPEETKKGRRHFHGSKELFLSDHLDVQSVDTIEGKCTVHSLENYMKLDVVGDDDFFCRLNYNSATGALTPDIVQVYCKCEMPYNPDEVMIQCDRCTDWFHPACIDMTVEEAERIDNFICESCSAEVQKKLQDSHSAARLPNTKVDTKRRQR*