AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017804


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017804 MtrunA17_Chr3g0145061 94.907 216 9 1 1 214 1 216 2.27e-153 427
MsaG017804 MtrunA17_Chr4g0035651 54.667 225 87 5 1 218 1 217 7.56e-73 223
MsaG017804 MtrunA17_Chr3g0142221 50.746 201 53 4 1 198 1 158 2.03e-59 188
MsaG017804 MtrunA17_Chr1g0155811 49.254 201 91 3 1 201 1 190 1.12e-54 178
MsaG017804 MtrunA17_Chr2g0314671 55.738 122 53 1 1 122 1 121 1.31e-39 139
MsaG017804 MtrunA17_Chr4g0033641 49.206 126 60 3 1 125 1 123 1.64e-34 124
MsaG017804 MtrunA17_Chr2g0329511 52.294 109 51 1 1 109 1 108 4.06e-33 121
MsaG017804 MtrunA17_Chr5g0405151 36.429 140 79 3 1 134 1 136 2.12e-23 94.4
MsaG017804 MtrunA17_Chr1g0150471 40.206 97 48 1 5 101 6 92 1.94e-17 80.5
MsaG017804 MtrunA17_Chr3g0136701 37.113 97 51 1 5 101 6 92 8.43e-16 75.9
MsaG017804 MtrunA17_Chr4g0072871 39.785 93 46 1 5 97 22 104 1.25e-15 75.9
MsaG017804 MtrunA17_Chr7g0220721 36.559 93 49 1 5 97 26 108 2.11e-13 69.7
MsaG017804 MtrunA17_Chr5g0429341 30.769 195 103 8 1 176 1 182 3.25e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017804 AT1G75540.1 49.083 218 99 4 1 206 1 218 1.88e-60 195
MsaG017804 AT4G39070.1 55.000 200 74 5 1 184 1 200 1.52e-55 179
MsaG017804 AT1G06040.2 52.206 136 62 2 1 136 1 133 7.87e-40 137
MsaG017804 AT1G78600.1 54.237 118 53 1 1 118 1 117 1.12e-38 137
MsaG017804 AT1G06040.1 52.206 136 62 2 1 136 1 133 1.72e-38 135
MsaG017804 AT1G78600.2 54.237 118 53 1 1 118 1 117 1.92e-38 137
MsaG017804 AT4G10240.1 51.799 139 57 3 1 133 1 135 5.97e-38 131
MsaG017804 AT2G31380.1 48.092 131 67 1 1 131 1 130 2.36e-35 127
MsaG017804 AT4G38960.1 39.316 117 67 2 1 117 1 113 4.42e-23 93.6
MsaG017804 AT4G38960.6 39.316 117 67 2 1 117 1 113 4.42e-23 93.6
MsaG017804 AT2G21320.1 44.000 100 52 2 1 100 1 96 3.90e-22 90.9
MsaG017804 AT2G24790.2 39.785 93 46 1 5 97 8 90 6.12e-17 78.2
MsaG017804 AT2G24790.1 38.144 97 50 1 5 101 8 94 2.11e-16 77.8
MsaG017804 AT5G24930.1 36.082 97 52 1 5 101 6 92 1.46e-15 76.3
MsaG017804 AT4G38960.3 28.750 160 67 3 1 117 1 156 3.00e-15 73.6
MsaG017804 AT5G15850.1 32.787 122 71 2 5 125 12 123 3.04e-14 72.4
MsaG017804 AT3G02380.1 34.286 105 57 2 5 109 16 108 2.28e-13 69.7
MsaG017804 AT2G47890.2 35.593 118 55 3 5 111 13 120 2.81e-12 65.5
MsaG017804 AT5G15840.1 31.132 106 61 2 5 110 20 113 4.70e-12 65.9
MsaG017804 AT2G47890.3 41.176 85 40 2 5 89 13 87 5.06e-12 65.5
MsaG017804 AT1G28050.1 34.653 101 56 1 2 102 6 96 5.34e-12 65.9
MsaG017804 AT5G15840.2 31.132 106 61 2 5 110 20 113 5.61e-12 65.1
MsaG017804 AT2G47890.1 41.176 85 40 2 5 89 13 87 5.83e-12 65.5
MsaG017804 AT2G33500.2 33.000 100 57 1 3 102 10 99 8.72e-12 65.1
MsaG017804 AT2G33500.1 33.000 100 57 1 3 102 10 99 8.82e-12 65.1

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTATGGCTGTACAGTTTC+AGG 0.212421 3:+113422292 MsaT017804.1:CDS
GATCTACTAGAGTAGTTTCT+TGG 0.265361 3:-113421509 None:intergenic
GAAGCTTTCAAGCAATTCTT+TGG 0.294858 3:+113421451 MsaT017804.1:CDS
ACTTCAAAATGGAAAGATTA+TGG 0.301261 3:+113422277 MsaT017804.1:CDS
GATGCTTCTTTCCCACCTAA+TGG 0.309132 3:+113421692 MsaT017804.1:CDS
CAAAAGTGAACACTTCAAAA+TGG 0.317889 3:+113422266 MsaT017804.1:CDS
ATAGAATCGCTCTATCTTCT+TGG 0.325288 3:-113421352 None:intergenic
CCCACCTAATGGTTTCTGTA+AGG 0.391077 3:+113421703 MsaT017804.1:CDS
TAAACCTTACAGAAACCATT+AGG 0.399460 3:-113421707 None:intergenic
ACTTGTTAACATGAATATCT+TGG 0.399770 3:-113422135 None:intergenic
TCTTGGTTCAGAGCCAATAC+TGG 0.403841 3:-113421492 None:intergenic
GCAGAAGCAGAATCATTATT+CGG 0.420688 3:+113422102 MsaT017804.1:intron
ATCACAAAGAGGGTAGTCTT+TGG 0.421009 3:-113421134 None:intergenic
ACCACTGAGCTAATATTAGA+AGG 0.428353 3:-113422226 None:intergenic
AACATACACAGAAACATAAC+AGG 0.440782 3:+113421411 MsaT017804.1:CDS
CATAACAGGTTTCTTCTCTC+TGG 0.447881 3:+113421425 MsaT017804.1:CDS
GTGTATGTTCATTTGCTCCA+TGG 0.455691 3:-113421397 None:intergenic
CATTTGCTCCATGGATTGGA+AGG 0.479724 3:-113421388 None:intergenic
ATACTGGTGGATGATGAATC+TGG 0.490828 3:-113421476 None:intergenic
GATTCATCATCCACCAGTAT+TGG 0.497576 3:+113421479 MsaT017804.1:CDS
AACGTGTGTACCTCTAGCTC+AGG 0.499247 3:+113422174 MsaT017804.1:CDS
TGTTCATTTGCTCCATGGAT+TGG 0.505507 3:-113421392 None:intergenic
GAATGTGACCTTCCAATCCA+TGG 0.511555 3:+113421380 MsaT017804.1:CDS
GAGGGTAGTCTTTGGAGTTG+AGG 0.523182 3:-113421126 None:intergenic
GATGAAGCTGCTCTTTGCCA+TGG 0.528890 3:+113421037 MsaT017804.1:CDS
GACAACATGGCTAGTGACAC+AGG 0.547231 3:+113421596 MsaT017804.1:CDS
TCTAATATTAGCTCAGTGGT+TGG 0.556823 3:+113422229 MsaT017804.1:CDS
AAATGCAAGTTCTTCTTGCA+TGG 0.564285 3:+113421568 MsaT017804.1:CDS
GAGCTAATATTAGAAGGAGT+AGG 0.569687 3:-113422220 None:intergenic
CTTTCTCACACACATCACAT+TGG 0.572968 3:-113420988 None:intergenic
GCTGATTCAGCCTGAGCTAG+AGG 0.574263 3:-113422184 None:intergenic
TGGTTCAGAGCCAATACTGG+TGG 0.580417 3:-113421489 None:intergenic
TGGTTGCAAGCTTGTTAGCA+CGG 0.587933 3:-113421078 None:intergenic
TTCATCTGAAGGACAGAACA+TGG 0.588007 3:-113421020 None:intergenic
AAGCTTGTTAGCACGGTGGA+TGG 0.588310 3:-113421071 None:intergenic
TACTTGATTGAAACAATACC+TGG 0.589710 3:+113421647 MsaT017804.1:CDS
GGTAGTCTTTGGAGTTGAGG+TGG 0.590439 3:-113421123 None:intergenic
ACCTTACAGAAACCATTAGG+TGG 0.591397 3:-113421704 None:intergenic
CAAAGAGCAGCTTCATCTGA+AGG 0.591587 3:-113421031 None:intergenic
GGATGGTATGATCACATCCA+TGG 0.613993 3:-113421054 None:intergenic
TACTGGTGGATGATGAATCT+GGG 0.618443 3:-113421475 None:intergenic
TTGCATGGCTGAAGACAACA+TGG 0.623591 3:+113421583 MsaT017804.1:CDS
CCTTACAGAAACCATTAGGT+GGG 0.623605 3:-113421703 None:intergenic
TCCTTCTAATATTAGCTCAG+TGG 0.624133 3:+113422225 MsaT017804.1:CDS
CTTGACAGATATCACAAAGA+GGG 0.634180 3:-113421144 None:intergenic
AAGTCTTCAAAACAGTAACC+AGG 0.647020 3:-113421665 None:intergenic
AGAGGTACACACGTTACTTG+AGG 0.647058 3:-113422166 None:intergenic
ACTTGACAGATATCACAAAG+AGG 0.665459 3:-113421145 None:intergenic
TTGCAAGCTTGTTAGCACGG+TGG 0.731754 3:-113421075 None:intergenic
TGATGTGTGTGAGAAAGCTG+AGG 0.739027 3:+113420994 MsaT017804.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 113420980 113422357 113420980 ID=MsaG017804
Chr3 mRNA 113420980 113422357 113420980 ID=MsaT017804.1;Parent=MsaG017804
Chr3 exon 113420980 113421165 113420980 ID=MsaT017804.1.exon1;Parent=MsaT017804.1
Chr3 CDS 113420980 113421165 113420980 ID=cds.MsaT017804.1;Parent=MsaT017804.1
Chr3 exon 113421329 113421724 113421329 ID=MsaT017804.1.exon2;Parent=MsaT017804.1
Chr3 CDS 113421329 113421724 113421329 ID=cds.MsaT017804.1;Parent=MsaT017804.1
Chr3 exon 113422106 113422357 113422106 ID=MsaT017804.1.exon3;Parent=MsaT017804.1
Chr3 CDS 113422106 113422357 113422106 ID=cds.MsaT017804.1;Parent=MsaT017804.1
Gene Sequence

>MsaT017804.1

ATGAAGATCCAATGTGATGTGTGTGAGAAAGCTGAGGCTTCCATGTTCTGTCCTTCAGATGAAGCTGCTCTTTGCCATGGATGTGATCATACCATCCACCGTGCTAACAAGCTTGCAACCAAACACACTCGTTTTTCTCTCGTCCACCTCAACTCCAAAGACTACCCTCTTTGTGATATCTGTCAAGAGAGGCGTGGATATCTATTTTGCCAAGAAGATAGAGCGATTCTATGCAGAGAATGTGACCTTCCAATCCATGGAGCAAATGAACATACACAGAAACATAACAGGTTTCTTCTCTCTGGTGTGAAGCTTTCAAGCAATTCTTTGGACCCAGATTCATCATCCACCAGTATTGGCTCTGAACCAAGAAACTACTCTAGTAGATCAAAAGCAAACATAATACCAAGATCAATTTCCAATGAAAATGCAAGTTCTTCTTGCATGGCTGAAGACAACATGGCTAGTGACACAGGTTCTGTTTCAACAAGCAGCATTTCTGAATACTTGATTGAAACAATACCTGGTTACTGTTTTGAAGACTTTCTTGATGCTTCTTTCCCACCTAATGGTTTCTGTAAGAAGCAGAATCATTATTCGGCATTTCAGTACCAAGATATTCATGTTAACAAGTTTTTATTTCCTCAAGTAACGTGTGTACCTCTAGCTCAGGCTGAATCAGCTCAACTAATTACTCCTACTCCTTCTAATATTAGCTCAGTGGTTGGAGTTAAAGAAATTTCAAAAGTGAACACTTCAAAATGGAAAGATTATGGCTGTACAGTTTCAGGAACTGATGCTGCATTCATAAAAAAATCTAGACACTTTCGATAA

Protein sequence

>MsaT017804.1

MKIQCDVCEKAEASMFCPSDEAALCHGCDHTIHRANKLATKHTRFSLVHLNSKDYPLCDICQERRGYLFCQEDRAILCRECDLPIHGANEHTQKHNRFLLSGVKLSSNSLDPDSSSTSIGSEPRNYSSRSKANIIPRSISNENASSSCMAEDNMASDTGSVSTSSISEYLIETIPGYCFEDFLDASFPPNGFCKKQNHYSAFQYQDIHVNKFLFPQVTCVPLAQAESAQLITPTPSNISSVVGVKEISKVNTSKWKDYGCTVSGTDAAFIKKSRHFR*