AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG017808


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017808 MtrunA17_Chr3g0145121 94.020 301 14 1 1 297 1 301 0.0 537
MsaG017808 MtrunA17_Chr4g0035891 56.369 314 100 9 1 296 1 295 2.58e-101 298
MsaG017808 MtrunA17_Chr8g0372291 41.304 276 135 7 21 281 25 288 1.33e-53 176
MsaG017808 MtrunA17_Chr8g0340401 37.868 272 130 7 39 280 33 295 7.08e-46 157
MsaG017808 MtrunA17_Chr5g0404981 39.385 325 150 9 1 282 51 371 1.40e-45 158
MsaG017808 MtrunA17_Chr3g0140331 41.199 267 126 9 36 281 67 323 8.31e-45 155
MsaG017808 MtrunA17_Chr1g0146231 34.653 303 153 8 18 281 8 304 2.60e-43 151
MsaG017808 MtrunA17_Chr1g0169431 62.637 91 34 0 190 280 151 241 3.22e-34 124
MsaG017808 MtrunA17_Chr7g0253101 38.333 240 97 9 80 275 20 252 1.05e-32 121
MsaG017808 MtrunA17_Chr1g0213161 35.841 226 110 6 103 293 9 234 1.07e-32 120
MsaG017808 MtrunA17_Chr1g0199671 76.923 65 15 0 211 275 242 306 1.14e-32 122
MsaG017808 MtrunA17_Chr6g0461591 80.000 65 13 0 211 275 192 256 2.38e-32 120
MsaG017808 MtrunA17_Chr2g0326771 68.493 73 23 0 203 275 208 280 3.82e-32 121
MsaG017808 MtrunA17_Chr4g0028961 73.134 67 18 0 211 277 232 298 2.56e-31 119
MsaG017808 MtrunA17_Chr5g0395391 62.025 79 30 0 202 280 261 339 3.40e-29 114
MsaG017808 MtrunA17_Chr5g0395391 54.945 91 41 0 192 282 149 239 4.90e-27 108
MsaG017808 MtrunA17_Chr7g0232671 58.730 63 26 0 210 272 186 248 2.76e-21 90.5
MsaG017808 MtrunA17_Chr7g0232701 55.556 63 28 0 210 272 72 134 5.81e-20 84.0
MsaG017808 MtrunA17_Chr7g0232681 31.844 179 107 6 100 276 30 195 9.91e-20 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG017808 AT4G36240.1 43.426 251 101 7 40 278 10 231 9.96e-53 172
MsaG017808 AT5G66320.2 39.936 313 145 9 8 278 5 316 3.09e-50 169
MsaG017808 AT5G66320.1 39.936 313 145 9 8 278 5 316 3.09e-50 169
MsaG017808 AT4G34680.1 42.466 292 113 11 6 286 7 254 1.83e-46 157
MsaG017808 AT4G34680.2 42.466 292 113 11 6 286 7 254 1.83e-46 157
MsaG017808 AT4G32890.1 39.858 281 132 13 21 280 2 266 4.26e-46 157
MsaG017808 AT3G60530.1 46.277 188 73 5 94 278 63 225 1.74e-41 144
MsaG017808 AT5G25830.1 38.028 284 125 12 40 280 13 288 3.68e-41 145
MsaG017808 AT2G45050.1 39.524 210 94 4 87 281 58 249 1.38e-38 137
MsaG017808 AT3G51080.1 70.000 90 23 2 211 296 221 310 1.96e-36 132
MsaG017808 AT3G54810.1 73.529 68 18 0 211 278 229 296 5.50e-32 121
MsaG017808 AT3G54810.2 73.529 68 18 0 211 278 229 296 5.50e-32 121
MsaG017808 AT3G24050.1 65.476 84 28 1 194 277 178 260 5.56e-32 120
MsaG017808 AT1G08010.2 63.636 77 28 0 201 277 210 286 6.01e-31 118
MsaG017808 AT1G08010.1 63.636 77 28 0 201 277 210 286 6.01e-31 118
MsaG017808 AT1G08010.3 63.636 77 28 0 201 277 210 286 6.01e-31 118
MsaG017808 AT1G08000.2 56.383 94 34 1 191 277 191 284 1.52e-29 114
MsaG017808 AT1G08000.1 56.383 94 34 1 191 277 191 284 1.52e-29 114
MsaG017808 AT3G45170.1 55.422 83 37 0 196 278 100 182 2.62e-27 105
MsaG017808 AT2G28340.1 61.538 65 25 0 213 277 193 257 7.15e-25 101

Find 83 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGTAACTGACCCTCTTCT+TGG 0.222887 3:-113495022 None:intergenic
GAGTAATTCGGTTGAGAAAA+TGG 0.282779 3:-113494608 None:intergenic
TTCTCATCTCCAACACTTTA+CGG 0.294944 3:-113495217 None:intergenic
CAACACTTTACGGTGGCTAT+TGG 0.312622 3:-113495207 None:intergenic
CTCAGCAAGTGGTAACCATT+TGG 0.349550 3:-113495286 None:intergenic
GAAAACCCAACCCTCGCGTT+TGG 0.352223 3:+113494910 MsaT017808.1:CDS
ACCGCCAACACGGTGGTTGC+CGG 0.362678 3:+113494424 MsaT017808.1:CDS
TTCTTGGGCCTCAAGAGCCT+CGG 0.380431 3:-113495006 None:intergenic
GGATGATGACGTGGCAGGTC+TGG 0.383333 3:+113494716 MsaT017808.1:intron
TGGACCGGAACCCGGTTTAC+CGG 0.387691 3:+113495256 MsaT017808.1:CDS
TTGGGTCTGAGCCGGTAAAC+CGG 0.389320 3:-113495267 None:intergenic
TGAGCCCAAACGCGAGGGTT+GGG 0.405637 3:-113494915 None:intergenic
AAAACCCAACCCTCGCGTTT+GGG 0.407277 3:+113494911 MsaT017808.1:CDS
GTGTTGGTTTGAATTTGAAC+CGG 0.408949 3:-113494792 None:intergenic
TGAATTTCACTGCTGAAAGT+TGG 0.418828 3:-113495182 None:intergenic
TTGTAACTGACCCTCTTCTT+GGG 0.419497 3:-113495021 None:intergenic
GGGTAGAGAAGAGATAGTTC+TGG 0.431245 3:-113494771 None:intergenic
TCAGCAAGTGGTAACCATTT+GGG 0.434470 3:-113495285 None:intergenic
GAATTTCACTGCTGAAAGTT+GGG 0.435175 3:-113495181 None:intergenic
ACTGCTGAAAGTTGGGCTAC+AGG 0.440191 3:-113495174 None:intergenic
TGCGGGGTTCGTTATAAATC+AGG 0.450021 3:+113495125 MsaT017808.1:CDS
ACTCACGGGAACGAGTAATT+CGG 0.455413 3:-113494620 None:intergenic
CTGAGCCCAAACGCGAGGGT+TGG 0.457925 3:-113494916 None:intergenic
TGAGCCGGTAAACCGGGTTC+CGG 0.459738 3:-113495260 None:intergenic
CAGTGGAGAACCGGTCCGTT+GGG 0.465140 3:+113495080 MsaT017808.1:CDS
AAGACGCCGCAGTGGAGAAC+CGG 0.467079 3:+113495071 MsaT017808.1:CDS
CTTTGAAACCGAGTTTAAGA+AGG 0.468143 3:+113494356 MsaT017808.1:CDS
AAACCCTTTGTAACGCGTGC+GGG 0.468327 3:+113495108 MsaT017808.1:CDS
ACCGGCAACCACCGTGTTGG+CGG 0.469757 3:-113494425 None:intergenic
CTTCACGGTTCTGGTCTTCG+TGG 0.473101 3:-113494883 None:intergenic
TCTTGGGCCTCAAGAGCCTC+GGG 0.483424 3:-113495005 None:intergenic
GGTAGAGAAGAGATAGTTCT+GGG 0.490371 3:-113494770 None:intergenic
TTCTTCTGTTTCTTCGCTGG+TGG 0.490862 3:-113494978 None:intergenic
ACTGCGGCGTCTTCTGCACC+TGG 0.496651 3:-113495061 None:intergenic
GCAGTGGAGAACCGGTCCGT+TGG 0.505231 3:+113495079 MsaT017808.1:CDS
CTCCTTGACTTCTCTAACGG+CGG 0.512576 3:+113494469 MsaT017808.1:CDS
ATGACGTGGCAGGTCTGGAA+TGG 0.521490 3:+113494721 MsaT017808.1:CDS
AACCCCGCACGCGTTACAAA+GGG 0.526943 3:-113495111 None:intergenic
TGAGAAAATGGAAGAATCAA+AGG 0.527539 3:-113494596 None:intergenic
AAGTGTTGGAGATGAGAAAG+AGG 0.539631 3:+113495222 MsaT017808.1:CDS
ACGACGGTGTAGTCATTGCC+AGG 0.543797 3:+113495043 MsaT017808.1:CDS
CTGAAAGTTGGGCTACAGGC+CGG 0.545068 3:-113495170 None:intergenic
TGGGTCTGAGCCGGTAAACC+GGG 0.551116 3:-113495266 None:intergenic
GGAATGGTTGGACCGGAACC+CGG 0.552734 3:+113495248 MsaT017808.1:CDS
GAGGAAAGGAATGGTTGGAC+CGG 0.555733 3:+113495241 MsaT017808.1:CDS
AACCCTTTGTAACGCGTGCG+GGG 0.556096 3:+113495109 MsaT017808.1:CDS
CAATAGCCACCGTAAAGTGT+TGG 0.557110 3:+113495208 MsaT017808.1:CDS
AGAAAGAGGAAAGGAATGGT+TGG 0.560194 3:+113495236 MsaT017808.1:CDS
ACAAACTCCCTTCTTAAACT+CGG 0.560314 3:-113494364 None:intergenic
GAACCCCGCACGCGTTACAA+AGG 0.560484 3:-113495112 None:intergenic
TTACAGGATGATGACGTGGC+AGG 0.560919 3:+113494711 MsaT017808.1:intron
GCTCTTGAGGCCCAAGAAGA+GGG 0.561687 3:+113495011 MsaT017808.1:CDS
GTAACCATTTGGGTCTGAGC+CGG 0.562666 3:-113495275 None:intergenic
TTGGAGATGAGAAAGAGGAA+AGG 0.563009 3:+113495227 MsaT017808.1:CDS
GGGCGCTGAGCCCAAACGCG+AGG 0.563588 3:-113494921 None:intergenic
TTTGAAACCGAGTTTAAGAA+GGG 0.567414 3:+113494357 MsaT017808.1:CDS
GGCTCTTGAGGCCCAAGAAG+AGG 0.567428 3:+113495010 MsaT017808.1:CDS
GAAGTCAAGGAGGTCGTCAA+CGG 0.569324 3:-113494458 None:intergenic
CACCGCCGTTAGAGAAGTCA+AGG 0.571693 3:-113494471 None:intergenic
ATCTTCTGAGAGTGATTGAG+AGG 0.572536 3:-113494548 None:intergenic
TTTACCGGCTCAGACCCAAA+TGG 0.575540 3:+113495271 MsaT017808.1:CDS
TGTTGGTTTGAATTTGAACC+GGG 0.578403 3:-113494791 None:intergenic
GATGAGAAAGAGGAAAGGAA+TGG 0.578460 3:+113495232 MsaT017808.1:CDS
TGACGTGGCAGGTCTGGAAT+GGG 0.589105 3:+113494722 MsaT017808.1:CDS
TCTCTTCTTCATCTCCGTCG+TGG 0.602523 3:-113494504 None:intergenic
AGAATCAAAGGAAGCGTCGG+TGG 0.602647 3:-113494584 None:intergenic
GGCGCTGAGCCCAAACGCGA+GGG 0.608307 3:-113494920 None:intergenic
GGAAGAATCAAAGGAAGCGT+CGG 0.616525 3:-113494587 None:intergenic
TTCACCGGCAACCACCGTGT+TGG 0.618211 3:-113494428 None:intergenic
CAATTTACAGGATGATGACG+TGG 0.618285 3:+113494707 MsaT017808.1:intron
CTAATGAATGACTCAGCAAG+TGG 0.621278 3:-113495297 None:intergenic
AGGTGCAGAAGACGCCGCAG+TGG 0.632590 3:+113495063 MsaT017808.1:CDS
AGAACTATCTCTTCTCTACC+CGG 0.632934 3:+113494773 MsaT017808.1:CDS
GGGTTCGTTATAAATCAGGT+CGG 0.635663 3:+113495129 MsaT017808.1:CDS
GTCAACGGAGAAATCTTCAC+CGG 0.637583 3:-113494443 None:intergenic
GACCTCCTTGACTTCTCTAA+CGG 0.644071 3:+113494466 MsaT017808.1:CDS
AAAACCCTTTGTAACGCGTG+CGG 0.650760 3:+113495107 MsaT017808.1:CDS
TCATCTCCAACACTTTACGG+TGG 0.668782 3:-113495214 None:intergenic
AACGGACCGGTTCTCCACTG+CGG 0.699688 3:-113495077 None:intergenic
TAGCTTCACCGCCAACACGG+TGG 0.733444 3:+113494417 MsaT017808.1:CDS
CGCCGTTAGAGAAGTCAAGG+AGG 0.737377 3:-113494468 None:intergenic
AAGAGGGTCAGTTACAACGA+CGG 0.739560 3:+113495027 MsaT017808.1:CDS
GGTGATATTCTTCACCACGA+CGG 0.777931 3:+113494490 MsaT017808.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 113494334 113495307 113494334 ID=MsaG017808
Chr3 mRNA 113494334 113495307 113494334 ID=MsaT017808.1;Parent=MsaG017808
Chr3 exon 113494334 113494636 113494334 ID=MsaT017808.1.exon1;Parent=MsaT017808.1
Chr3 CDS 113494334 113494636 113494334 ID=cds.MsaT017808.1;Parent=MsaT017808.1
Chr3 exon 113494717 113495307 113494717 ID=MsaT017808.1.exon2;Parent=MsaT017808.1
Chr3 CDS 113494717 113495307 113494717 ID=cds.MsaT017808.1;Parent=MsaT017808.1
Gene Sequence

>MsaT017808.1

ATGGAGTTTGCAGTAGCAAAAGCTTTGAAACCGAGTTTAAGAAGGGAGTTTGTTTTTCAGCAAACTCTCGGCGAGGAACTTTTTAGCTTCACCGCCAACACGGTGGTTGCCGGTGAAGATTTCTCCGTTGACGACCTCCTTGACTTCTCTAACGGCGGTGATATTCTTCACCACGACGGAGATGAAGAAGAGAAAGATAGCAGTTTATCTCTTTCCTCTCAATCACTCTCAGAAGATAACAACTCAAATTCCACCGACGCTTCCTTTGATTCTTCCATTTTCTCAACCGAATTACTCGTTCCCGATGATGACGTGGCAGGTCTGGAATGGGTTTCACACTTTGTAGATGATTCTCTCCCAGAACTATCTCTTCTCTACCCGGTTCAAATTCAAACCAACACGTGTCCTCAAAAACATGGAACTCAGCATGGAAAAACTTTACGTTTTTCAACTGAAAAAATGAAAATTACCACGAAGACCAGAACCGTGAAGAATAGAAAACCCAACCCTCGCGTTTGGGCTCAGCGCCCGTTTTTTCCGTCACCGTCTGTTGTTTTTGGGGGACCACCAGCGAAGAAACAGAAGAAAAAGCCCGAGGCTCTTGAGGCCCAAGAAGAGGGTCAGTTACAACGACGGTGTAGTCATTGCCAGGTGCAGAAGACGCCGCAGTGGAGAACCGGTCCGTTGGGTGCTAAAACCCTTTGTAACGCGTGCGGGGTTCGTTATAAATCAGGTCGGCTTTTTTCGGAGTATAGACCGGCCTGTAGCCCAACTTTCAGCAGTGAAATTCATTCCAATAGCCACCGTAAAGTGTTGGAGATGAGAAAGAGGAAAGGAATGGTTGGACCGGAACCCGGTTTACCGGCTCAGACCCAAATGGTTACCACTTGCTGA

Protein sequence

>MsaT017808.1

MEFAVAKALKPSLRREFVFQQTLGEELFSFTANTVVAGEDFSVDDLLDFSNGGDILHHDGDEEEKDSSLSLSSQSLSEDNNSNSTDASFDSSIFSTELLVPDDDVAGLEWVSHFVDDSLPELSLLYPVQIQTNTCPQKHGTQHGKTLRFSTEKMKITTKTRTVKNRKPNPRVWAQRPFFPSPSVVFGGPPAKKQKKKPEALEAQEEGQLQRRCSHCQVQKTPQWRTGPLGAKTLCNACGVRYKSGRLFSEYRPACSPTFSSEIHSNSHRKVLEMRKRKGMVGPEPGLPAQTQMVTTC*