AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG018569

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG018569 MtrunA17_Chr4g0008551 97.872 94 2 0 1 94 1 94 1.62e-62 193
MsaG018569 MtrunA17_Chr3g0133601 72.881 59 16 0 36 94 35 93 2.96e-27 99.8
MsaG018569 MtrunA17_Chr4g0053431 66.102 59 20 0 36 94 3 61 9.76e-25 94.0
MsaG018569 MtrunA17_Chr5g0401731 64.407 59 21 0 36 94 13 71 1.08e-23 92.0
MsaG018569 MtrunA17_Chr6g0454921 64.407 59 21 0 36 94 13 71 3.53e-23 88.6
MsaG018569 MtrunA17_Chr5g0437321 61.194 67 26 0 28 94 9 75 1.21e-22 89.0
MsaG018569 MtrunA17_Chr6g0460671 56.250 64 28 0 31 94 92 155 4.86e-21 85.5
MsaG018569 MtrunA17_Chr6g0460661 49.275 69 35 0 26 94 4 72 4.40e-20 79.3
MsaG018569 MtrunA17_Chr3g0125801 61.404 57 22 0 38 94 16 72 5.59e-20 81.6
MsaG018569 MtrunA17_Chr5g0417621 52.941 68 32 0 27 94 123 190 1.42e-19 82.0
MsaG018569 MtrunA17_Chr6g0480581 57.895 57 24 0 38 94 4 60 6.44e-19 78.2
MsaG018569 MtrunA17_Chr7g0254261 57.895 57 24 0 38 94 89 145 1.44e-18 78.6
MsaG018569 MtrunA17_Chr4g0042501 53.125 64 30 0 31 94 98 161 5.69e-18 77.4
MsaG018569 MtrunA17_Chr3g0104501 40.206 97 46 2 1 89 92 184 6.22e-16 69.7
MsaG018569 MtrunA17_Chr1g0157731 48.276 58 30 0 37 94 90 147 3.78e-15 69.3
MsaG018569 MtrunA17_Chr4g0058991 51.724 58 28 0 37 94 208 265 5.35e-15 68.9
MsaG018569 MtrunA17_Chr7g0238631 50.000 58 29 0 37 94 112 169 6.57e-15 68.9
MsaG018569 MtrunA17_Chr5g0425591 50.000 64 31 1 32 94 5 68 7.84e-15 64.3
MsaG018569 MtrunA17_Chr3g0139671 48.276 58 30 0 37 94 84 141 7.88e-15 68.6
MsaG018569 MtrunA17_Chr5g0417821 53.448 58 26 1 38 94 9 66 1.62e-14 67.0
MsaG018569 MtrunA17_Chr4g0013361 48.571 70 31 2 30 98 4 69 2.99e-14 66.2
MsaG018569 MtrunA17_Chr7g0233161 50.909 55 27 0 35 89 296 350 3.90e-14 66.2
MsaG018569 MtrunA17_Chr5g0440781 51.724 58 27 1 38 94 14 71 6.62e-14 63.9
MsaG018569 MtrunA17_Chr5g0429661 46.552 58 31 0 37 94 121 178 6.68e-14 65.9
MsaG018569 MtrunA17_Chr4g0046181 48.276 58 30 0 37 94 220 277 8.35e-14 65.1
MsaG018569 MtrunA17_Chr8g0334881 52.830 53 24 1 38 89 12 64 1.19e-13 64.7
MsaG018569 MtrunA17_Chr5g0441141 51.724 58 27 1 38 94 14 71 1.52e-13 64.3
MsaG018569 MtrunA17_Chr5g0440761 51.724 58 27 1 38 94 14 71 1.69e-13 63.9
MsaG018569 MtrunA17_Chr3g0117511 50.000 54 27 0 37 90 182 235 1.92e-13 64.7
MsaG018569 MtrunA17_Chr2g0322521 49.153 59 27 2 38 94 31 88 2.41e-13 64.3
MsaG018569 MtrunA17_Chr5g0397841 48.276 58 30 0 37 94 109 166 4.84e-13 63.5
MsaG018569 MtrunA17_Chr7g0228531 52.830 53 25 0 37 89 219 271 8.14e-13 62.8
MsaG018569 MtrunA17_Chr3g0099141 46.552 58 30 1 38 94 42 99 1.11e-12 60.1
MsaG018569 MtrunA17_Chr2g0327821 52.542 59 25 2 38 94 8 65 1.41e-12 61.2
MsaG018569 MtrunA17_Chr3g0103281 50.000 58 28 1 38 94 14 71 1.59e-12 61.6
MsaG018569 MtrunA17_Chr4g0030881 48.485 66 24 2 38 94 9 73 1.78e-12 61.2
MsaG018569 MtrunA17_Chr4g0042611 50.000 58 28 1 38 94 14 71 1.81e-12 58.2
MsaG018569 MtrunA17_Chr5g0441131 45.312 64 34 1 32 94 5 68 1.93e-12 60.1
MsaG018569 MtrunA17_Chr4g0039971 50.000 64 28 3 33 94 11 72 2.31e-12 61.2
MsaG018569 MtrunA17_Chr7g0228481 52.830 53 25 0 37 89 219 271 2.35e-12 61.6
MsaG018569 MtrunA17_Chr3g0080861 50.000 60 27 2 38 95 39 97 4.82e-12 60.5
MsaG018569 MtrunA17_Chr1g0167691 47.458 59 30 1 38 95 14 72 5.09e-12 60.1
MsaG018569 MtrunA17_Chr6g0453751 46.753 77 29 5 28 94 13 87 5.86e-12 57.8
MsaG018569 MtrunA17_Chr7g0261281 48.276 58 29 1 38 94 14 71 5.98e-12 60.1
MsaG018569 MtrunA17_Chr4g0060721 50.000 58 28 1 38 94 15 72 7.77e-12 59.7
MsaG018569 MtrunA17_Chr2g0311221 50.000 58 28 1 38 94 16 73 7.83e-12 59.7
MsaG018569 MtrunA17_Chr7g0254411 50.847 59 26 2 38 94 14 71 1.02e-11 59.7
MsaG018569 MtrunA17_Chr1g0187761 48.333 60 28 2 38 95 14 72 1.17e-11 58.9
MsaG018569 MtrunA17_Chr1g0194301 52.542 59 25 2 38 94 35 92 1.42e-11 58.9
MsaG018569 MtrunA17_Chr3g0088461 47.222 72 36 2 25 94 10 81 1.61e-11 58.9
MsaG018569 MtrunA17_Chr7g0217221 50.847 59 26 2 38 94 14 71 1.67e-11 58.5
MsaG018569 MtrunA17_Chr6g0453211 48.276 58 29 1 38 94 14 71 1.89e-11 58.5
MsaG018569 MtrunA17_Chr3g0115821 48.438 64 29 3 33 94 10 71 1.99e-11 58.9
MsaG018569 MtrunA17_Chr7g0274581 48.276 58 29 1 38 94 14 71 2.56e-11 57.4
MsaG018569 MtrunA17_Chr4g0048511 43.939 66 35 2 30 94 8 72 2.68e-11 58.5
MsaG018569 MtrunA17_Chr3g0117091 49.153 59 27 2 38 94 14 71 3.01e-11 58.2
MsaG018569 MtrunA17_Chr1g0209661 43.103 58 32 1 38 94 29 86 3.30e-11 58.2
MsaG018569 MtrunA17_Chr1g0180391 47.619 63 30 2 34 94 17 78 3.98e-11 57.4
MsaG018569 MtrunA17_Chr5g0410961 50.000 58 28 1 38 94 14 71 4.35e-11 57.8
MsaG018569 MtrunA17_Chr6g0453471 48.276 58 29 1 38 94 14 71 6.10e-11 57.4
MsaG018569 MtrunA17_Chr2g0281071 43.939 66 35 2 30 94 8 72 6.25e-11 57.4
MsaG018569 MtrunA17_Chr7g0264641 45.161 62 32 2 38 97 14 75 7.96e-11 56.6
MsaG018569 MtrunA17_Chr4g0072751 50.847 59 27 2 38 94 14 72 8.35e-11 57.0
MsaG018569 MtrunA17_Chr5g0395221 49.231 65 31 2 31 94 8 71 9.28e-11 53.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG018569 AT3G55730.1 53.571 112 34 2 1 94 1 112 6.71e-30 110
MsaG018569 AT2G39880.1 58.333 84 35 0 11 94 23 106 9.00e-28 103
MsaG018569 AT3G09230.1 71.186 59 17 0 36 94 53 111 1.74e-24 95.5
MsaG018569 AT5G67300.1 63.333 60 22 0 36 95 4 63 2.34e-24 94.0
MsaG018569 AT4G37260.1 66.102 59 20 0 36 94 11 69 8.70e-24 92.8
MsaG018569 AT2G23290.1 65.000 60 21 0 36 95 11 70 6.12e-23 90.5
MsaG018569 AT3G50060.1 64.407 59 21 0 36 94 4 62 6.42e-23 90.1
MsaG018569 AT1G69560.2 54.545 66 30 0 29 94 114 179 1.61e-20 84.3
MsaG018569 AT1G69560.1 54.545 66 30 0 29 94 98 163 1.71e-20 84.0
MsaG018569 AT1G17950.1 63.158 57 21 0 38 94 5 61 3.25e-20 82.0
MsaG018569 AT4G33450.1 59.649 57 23 0 38 94 19 75 3.59e-20 82.0
MsaG018569 AT5G17800.1 42.857 98 51 2 2 94 52 149 9.84e-20 82.0
MsaG018569 AT1G26780.2 59.649 57 23 0 38 94 98 154 2.95e-19 80.9
MsaG018569 AT1G26780.1 59.649 57 23 0 38 94 98 154 6.61e-19 79.3
MsaG018569 AT1G73410.1 59.649 57 23 0 38 94 6 62 1.01e-18 78.2
MsaG018569 AT1G73410.2 59.649 57 23 0 38 94 6 62 1.19e-18 77.0
MsaG018569 AT3G29020.2 50.877 57 28 0 38 94 67 123 1.65e-15 70.1
MsaG018569 AT3G29020.1 50.877 57 28 0 38 94 65 121 1.74e-15 69.3
MsaG018569 AT5G11510.3 46.552 58 31 0 37 94 80 137 4.64e-15 69.7
MsaG018569 AT5G11510.1 46.552 58 31 0 37 94 80 137 4.64e-15 69.7
MsaG018569 AT5G11510.4 46.552 58 31 0 37 94 80 137 4.64e-15 69.7
MsaG018569 AT5G11510.5 46.552 58 31 0 37 94 80 137 5.35e-15 69.3
MsaG018569 AT5G11510.2 46.552 58 31 0 37 94 80 137 5.36e-15 69.3
MsaG018569 AT3G27785.1 47.541 61 32 0 34 94 185 245 1.73e-14 67.8
MsaG018569 AT4G18770.1 44.615 65 36 0 30 94 209 273 2.07e-14 67.8
MsaG018569 AT5G58850.1 49.153 59 30 0 32 90 99 157 2.52e-14 67.4
MsaG018569 AT5G40360.1 45.614 57 31 0 38 94 158 214 9.69e-14 65.9
MsaG018569 AT3G09370.2 42.857 63 36 0 37 99 134 196 1.19e-13 65.5
MsaG018569 AT3G09370.1 42.857 63 36 0 37 99 129 191 1.26e-13 65.5
MsaG018569 AT5G02320.3 46.552 58 31 0 37 94 66 123 1.47e-13 65.5
MsaG018569 AT3G09370.3 42.857 63 36 0 37 99 142 204 1.54e-13 65.1
MsaG018569 AT5G02320.1 46.552 58 31 0 37 94 126 183 1.74e-13 65.1
MsaG018569 AT5G02320.2 46.552 58 31 0 37 94 126 183 1.74e-13 65.1
MsaG018569 AT4G32730.1 46.552 58 31 0 37 94 86 143 2.86e-13 64.7
MsaG018569 AT4G32730.5 46.552 58 31 0 37 94 86 143 3.14e-13 64.3
MsaG018569 AT4G32730.4 46.552 58 31 0 37 94 86 143 3.14e-13 64.3
MsaG018569 AT4G32730.3 46.552 58 31 0 37 94 86 143 3.14e-13 64.3
MsaG018569 AT4G32730.2 46.552 58 31 0 37 94 86 143 3.14e-13 64.3
MsaG018569 AT5G59780.3 50.847 59 26 2 38 94 10 67 6.09e-13 62.8
MsaG018569 AT3G46130.1 52.542 59 25 2 38 94 11 68 6.43e-13 62.8
MsaG018569 AT3G60460.1 52.830 53 24 1 38 89 13 65 9.03e-13 62.8
MsaG018569 AT1G79180.1 53.968 63 27 2 35 95 12 74 1.36e-12 62.0
MsaG018569 AT5G62470.1 49.123 57 29 0 38 94 14 70 1.55e-12 62.4
MsaG018569 AT5G55020.1 47.458 59 30 1 38 95 28 86 1.66e-12 62.4
MsaG018569 AT2G47190.1 57.627 59 22 2 38 94 22 79 1.85e-12 61.6
MsaG018569 AT5G14750.1 50.000 68 32 2 28 94 9 75 1.99e-12 60.8
MsaG018569 AT2G31180.1 48.276 58 29 1 38 94 14 71 2.27e-12 61.2
MsaG018569 AT2G32460.1 46.341 82 36 4 15 94 2 77 2.36e-12 62.0
MsaG018569 AT5G56110.1 48.276 58 29 1 38 94 14 71 3.15e-12 61.2
MsaG018569 AT5G11050.1 43.103 58 33 0 37 94 104 161 3.53e-12 61.2
MsaG018569 AT5G39700.1 35.802 81 51 1 15 94 32 112 3.65e-12 59.7
MsaG018569 AT1G25340.1 44.928 69 35 2 28 94 10 77 4.28e-12 60.8
MsaG018569 AT3G23250.1 50.000 58 28 1 38 94 14 71 5.97e-12 60.5
MsaG018569 AT3G53200.1 50.847 59 26 2 38 94 11 68 6.21e-12 60.1
MsaG018569 AT1G25340.2 43.284 67 34 1 28 94 10 72 6.55e-12 60.1
MsaG018569 AT2G32460.2 50.847 59 26 2 38 94 8 65 6.65e-12 60.5
MsaG018569 AT1G25340.3 43.284 67 34 1 28 94 10 72 7.53e-12 60.1
MsaG018569 AT3G61250.1 48.333 60 28 2 38 95 14 72 8.19e-12 60.1
MsaG018569 AT1G66380.2 43.939 66 36 1 30 94 2 67 8.86e-12 57.4
MsaG018569 AT1G74650.1 53.448 58 26 1 38 94 14 71 8.89e-12 60.1
MsaG018569 AT1G06180.1 52.542 59 25 2 38 94 14 71 9.24e-12 59.7
MsaG018569 AT3G30210.1 50.847 59 26 2 38 94 29 86 1.14e-11 59.7
MsaG018569 AT1G56160.1 49.206 63 28 3 35 94 12 73 1.80e-11 58.9
MsaG018569 AT4G26930.1 41.429 70 40 1 26 94 9 78 2.19e-11 58.9
MsaG018569 AT3G11440.2 36.471 85 53 1 11 94 16 100 3.64e-11 58.5
MsaG018569 AT3G11440.3 46.552 58 30 1 38 94 43 100 3.75e-11 58.5
MsaG018569 AT3G11440.4 46.552 58 30 1 38 94 43 100 3.75e-11 58.5
MsaG018569 AT3G11440.1 46.552 58 30 1 38 94 43 100 3.75e-11 58.5
MsaG018569 AT5G06100.1 40.000 85 46 2 15 94 7 91 4.41e-11 58.2
MsaG018569 AT1G08810.1 48.276 58 29 1 38 94 14 71 4.41e-11 57.8
MsaG018569 AT1G66380.1 43.939 66 36 1 30 94 2 67 4.46e-11 56.2
MsaG018569 AT5G06100.5 48.276 58 29 1 38 94 34 91 5.29e-11 58.2
MsaG018569 AT5G06100.2 48.276 58 29 1 38 94 34 91 5.29e-11 58.2
MsaG018569 AT5G06100.3 48.276 58 29 1 38 94 34 91 5.29e-11 58.2
MsaG018569 AT5G06100.4 48.276 58 29 1 38 94 34 91 5.29e-11 58.2
MsaG018569 AT3G28910.1 48.276 58 29 1 38 94 14 71 5.32e-11 57.8
MsaG018569 AT5G62470.2 50.000 58 28 1 38 94 14 71 5.55e-11 57.8
MsaG018569 AT3G47600.1 50.000 58 28 1 38 94 14 71 5.71e-11 57.8
MsaG018569 AT3G01140.2 50.000 58 28 1 38 94 14 71 5.77e-11 57.8
MsaG018569 AT4G05100.1 46.552 58 30 1 38 94 15 72 6.69e-11 57.8
MsaG018569 AT3G01140.1 50.000 58 28 1 38 94 57 114 7.92e-11 57.4
MsaG018569 AT4G01680.3 49.153 59 27 2 38 94 14 71 9.82e-11 56.6
MsaG018569 AT4G01680.1 49.153 59 27 2 38 94 14 71 9.85e-11 57.0

Find 35 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTGCTCGTGGAATTTC+TGG 0.157255 4:+12055975 MsaT018569.1:CDS
CTGAGAATGGCGTCTTCTTC+AGG 0.289480 4:-12055915 None:intergenic
AGAGTTGGTGGTTGTTGTTC+TGG 0.316358 4:-12055795 None:intergenic
GTCAGTTCGGTGCTAGAAAC+TGG 0.340339 4:+12055949 MsaT018569.1:CDS
AGCTTGATCCTTCTGTTAAA+CGG 0.393224 4:+12056036 MsaT018569.1:CDS
GGAAGTCGTGCCGGCTCCGA+TGG 0.433047 4:+12056006 MsaT018569.1:CDS
CGTGGAATTTCTGGAAGATC+GGG 0.442466 4:+12055984 MsaT018569.1:CDS
AGTCGTCTTGTTAGTCAGTT+CGG 0.460173 4:+12055936 MsaT018569.1:CDS
GACGAAGGTTTAGCCGCATT+AGG 0.461626 4:+12055843 MsaT018569.1:CDS
TCGTGGAATTTCTGGAAGAT+CGG 0.489928 4:+12055983 MsaT018569.1:CDS
AACAAGTTTCGTACCAGTGA+AGG 0.498645 4:-12056063 None:intergenic
ACGGCGGTGCACGACGGAAG+CGG 0.506031 4:+12055819 MsaT018569.1:CDS
TCGTGCACCGCCGTGAGAGT+TGG 0.511514 4:-12055810 None:intergenic
ACTAACAAGACGACTGAGAA+TGG 0.521928 4:-12055928 None:intergenic
AACTGGAGTTTGATTGCTCG+TGG 0.529814 4:+12055966 MsaT018569.1:CDS
TGCACCGCCGTGAGAGTTGG+TGG 0.533257 4:-12055807 None:intergenic
GAAGATCGGGGAAGTCGTGC+CGG 0.535316 4:+12055997 MsaT018569.1:CDS
AAGGGTTTCCGTTTAACAGA+AGG 0.537963 4:-12056044 None:intergenic
GCATTAGGCGAAGGCGGCGA+AGG 0.538199 4:+12055858 MsaT018569.1:CDS
GTTAAACGGAAACCCTTCAC+TGG 0.542263 4:+12056050 MsaT018569.1:CDS
GCTGATTACACCATCGGAGC+CGG 0.561787 4:-12056016 None:intergenic
GGTTTAGCCGCATTAGGCGA+AGG 0.563573 4:+12055849 MsaT018569.1:CDS
ACAAGTTTCGTACCAGTGAA+GGG 0.577594 4:-12056062 None:intergenic
GATCAAGCTGATTACACCAT+CGG 0.607608 4:-12056022 None:intergenic
AGTAGCGGTAGCAAAGTGAA+AGG 0.608608 4:+12055882 MsaT018569.1:CDS
ACAACAACCACCAACTCTCA+CGG 0.609856 4:+12055800 MsaT018569.1:CDS
TTCGCCGCCTTCGCCTAATG+CGG 0.619488 4:-12055856 None:intergenic
GTAGCAAAGTGAAAGGACCT+TGG 0.631627 4:+12055889 MsaT018569.1:CDS
GAAGGCGGCGAAGGAAGTAG+CGG 0.640817 4:+12055867 MsaT018569.1:CDS
CACGACGGAAGCGGAGACGA+AGG 0.641211 4:+12055828 MsaT018569.1:CDS
ACTCTCACGGCGGTGCACGA+CGG 0.656704 4:+12055813 MsaT018569.1:CDS
TTAGCCGCATTAGGCGAAGG+CGG 0.661776 4:+12055852 MsaT018569.1:CDS
GCGTCTTCTTCAGGAGACCA+AGG 0.675143 4:-12055906 None:intergenic
GTGGAATTTCTGGAAGATCG+GGG 0.703083 4:+12055985 MsaT018569.1:CDS
ACAACCACCAACTCTCACGG+CGG 0.782687 4:+12055803 MsaT018569.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 12055789 12056100 12055789 ID=MsaG018569
Chr4 mRNA 12055789 12056100 12055789 ID=MsaT018569.1;Parent=MsaG018569
Chr4 exon 12055789 12056100 12055789 ID=MsaT018569.1.exon1;Parent=MsaT018569.1
Chr4 CDS 12055789 12056100 12055789 ID=cds.MsaT018569.1;Parent=MsaT018569.1
Gene Sequence

>MsaT018569.1

ATGGAACCAGAACAACAACCACCAACTCTCACGGCGGTGCACGACGGAAGCGGAGACGAAGGTTTAGCCGCATTAGGCGAAGGCGGCGAAGGAAGTAGCGGTAGCAAAGTGAAAGGACCTTGGTCTCCTGAAGAAGACGCCATTCTCAGTCGTCTTGTTAGTCAGTTCGGTGCTAGAAACTGGAGTTTGATTGCTCGTGGAATTTCTGGAAGATCGGGGAAGTCGTGCCGGCTCCGATGGTGTAATCAGCTTGATCCTTCTGTTAAACGGAAACCCTTCACTGGTACGAAACTTGTTAATGATCGCTTCTAA
Protein sequence

>MsaT018569.1

MEPEQQPPTLTAVHDGSGDEGLAALGEGGEGSSGSKVKGPWSPEEDAILSRLVSQFGARNWSLIARGISGRSGKSCRLRWCNQLDPSVKRKPFTGTKLVNDRF*