AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019029


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019029 MtrunA17_Chr1g0201311 94.828 116 6 0 84 199 116 231 1.23e-73 231
MsaG019029 MtrunA17_Chr1g0201621 93.966 116 7 0 84 199 116 231 8.23e-73 229
MsaG019029 MtrunA17_Chr7g0220311 97.143 70 2 0 1 70 2481 2550 4.63e-39 144
MsaG019029 MtrunA17_Chr3g0125251 38.971 136 78 2 84 218 1356 1487 2.24e-24 101
MsaG019029 MtrunA17_Chr2g0305371 44.792 96 52 1 101 196 382 476 2.63e-21 92.4
MsaG019029 MtrunA17_Chr6g0477001 39.130 138 75 3 84 213 144 280 1.57e-20 89.0
MsaG019029 MtrunA17_Chr7g0235081 47.126 87 46 0 84 170 226 312 1.58e-15 74.7
MsaG019029 MtrunA17_Chr2g0297191 33.654 104 68 1 84 187 1064 1166 4.07e-14 71.6
MsaG019029 MtrunA17_Chr5g0401921 44.318 88 47 1 84 171 746 831 3.13e-13 68.9
MsaG019029 MtrunA17_Chr1g0194631 43.678 87 47 1 84 170 642 726 4.62e-13 68.6
MsaG019029 MtrunA17_Chr1g0149611 33.835 133 76 2 38 170 365 485 5.06e-13 68.2
MsaG019029 MtrunA17_Chr8g0347071 37.500 104 61 3 91 191 846 948 4.02e-12 65.5
MsaG019029 MtrunA17_Chr3g0126701 37.500 104 61 3 91 191 960 1062 4.43e-12 65.5
MsaG019029 MtrunA17_Chr1g0147121 39.560 91 52 2 104 191 943 1033 6.76e-12 65.1
MsaG019029 MtrunA17_Chr1g0149581 46.875 64 34 0 107 170 1580 1643 7.09e-12 65.1
MsaG019029 MtrunA17_Chr7g0221611 46.875 64 34 0 107 170 961 1024 3.10e-11 63.2
MsaG019029 MtrunA17_Chr7g0275611 38.318 107 56 4 91 191 861 963 6.90e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019029 AT1G76710.2 78.448 116 25 0 84 199 121 236 1.05e-58 193
MsaG019029 AT1G76710.1 78.448 116 25 0 84 199 121 236 1.05e-58 193
MsaG019029 AT1G76710.4 78.448 116 25 0 84 199 121 236 1.76e-58 193
MsaG019029 AT1G76710.3 78.448 116 25 0 84 199 121 236 2.58e-58 193
MsaG019029 AT1G77300.1 44.545 110 60 1 84 192 1058 1167 1.53e-24 102
MsaG019029 AT1G77300.2 44.545 110 60 1 84 192 1058 1167 1.58e-24 102
MsaG019029 AT3G02260.1 67.606 71 23 0 1 71 2486 2556 2.44e-23 99.4
MsaG019029 AT3G02260.2 67.143 70 23 0 1 70 2469 2538 1.45e-22 97.1
MsaG019029 AT3G02260.3 67.143 70 23 0 1 70 2470 2539 1.45e-22 97.1
MsaG019029 AT3G02260.4 67.143 70 23 0 1 70 2479 2548 1.45e-22 97.1
MsaG019029 AT4G30860.1 43.478 115 60 3 84 196 358 469 8.79e-22 94.0
MsaG019029 AT2G44150.1 36.806 144 86 3 84 225 148 288 2.84e-18 83.2
MsaG019029 AT3G59960.3 44.000 100 51 2 95 194 158 252 1.34e-17 80.5
MsaG019029 AT3G59960.2 44.000 100 51 2 95 194 158 252 1.34e-17 80.5
MsaG019029 AT3G59960.4 44.000 100 51 2 95 194 158 252 1.34e-17 80.5
MsaG019029 AT3G59960.1 44.000 100 51 2 95 194 158 252 2.31e-17 80.1
MsaG019029 AT5G42400.7 36.538 104 65 1 84 187 1281 1383 4.68e-16 77.8
MsaG019029 AT5G42400.2 36.538 104 65 1 84 187 1281 1383 4.68e-16 77.8
MsaG019029 AT5G42400.8 36.538 104 65 1 84 187 1281 1383 4.68e-16 77.8
MsaG019029 AT5G42400.5 36.538 104 65 1 84 187 1281 1383 4.68e-16 77.8
MsaG019029 AT5G42400.4 36.538 104 65 1 84 187 1281 1383 4.68e-16 77.8
MsaG019029 AT5G42400.1 36.538 104 65 1 84 187 1281 1383 4.68e-16 77.8
MsaG019029 AT5G42400.3 36.538 104 65 1 84 187 1281 1383 4.68e-16 77.8
MsaG019029 AT5G42400.6 36.538 104 65 1 84 187 1281 1383 4.68e-16 77.8
MsaG019029 AT2G23380.1 45.455 88 46 1 84 171 784 869 4.61e-14 71.6
MsaG019029 AT2G23380.2 45.455 88 46 1 84 171 557 642 5.87e-14 71.2
MsaG019029 AT4G27910.1 38.462 104 60 3 91 191 924 1026 2.85e-12 66.6
MsaG019029 AT4G02020.1 42.529 87 48 1 84 170 739 823 2.93e-12 66.6
MsaG019029 AT3G61740.1 39.623 106 57 4 91 191 914 1017 8.40e-12 65.1
MsaG019029 AT3G61740.2 39.623 106 57 4 91 191 878 981 9.16e-12 65.1
MsaG019029 AT1G05830.2 38.636 88 53 1 84 170 951 1038 1.84e-11 64.3
MsaG019029 AT1G05830.4 38.636 88 53 1 84 170 951 1038 1.84e-11 64.3
MsaG019029 AT1G05830.1 38.636 88 53 1 84 170 951 1038 1.84e-11 64.3
MsaG019029 AT1G05830.3 38.636 88 53 1 84 170 951 1038 1.84e-11 64.3
MsaG019029 AT2G31650.1 45.588 68 37 0 107 174 954 1021 3.55e-11 63.2

Find 38 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGATTTATAAACCATTCT+TGG 0.218332 4:-22044566 MsaT019029.1:intron
GACCTTGAGTTTCATAAGTA+TGG 0.226901 4:+22044739 None:intergenic
AGATATAACAATGTTGTTCA+TGG 0.318271 4:+22045840 None:intergenic
GCACTGGAAGATATTCCTAT+TGG 0.334570 4:-22044345 MsaT019029.1:CDS
GCCCTCCATGTAAAAGATTC+AGG 0.335082 4:-22045781 MsaT019029.1:CDS
CAAATTGTGAGACAAGAAAA+TGG 0.341946 4:-22044401 MsaT019029.1:CDS
TTGAAGTAGTTTACCAAGAA+TGG 0.363419 4:+22044553 None:intergenic
TTGTTCATGGTTTGAGTGTC+GGG 0.416109 4:+22045853 None:intergenic
CTCAAATGTTCTGAATTCCT+TGG 0.421542 4:-22044252 MsaT019029.1:CDS
AGACCCACTAATAGCTTGAA+TGG 0.421574 4:-22044073 MsaT019029.1:CDS
TTCTTCAGTAATACAACAGC+AGG 0.454164 4:+22045742 None:intergenic
TATTCCTATTGGAACTGAAC+TGG 0.465129 4:-22044334 MsaT019029.1:CDS
TATGCCAGTTCAGTTCCAAT+AGG 0.481653 4:+22044330 None:intergenic
TTATTGTTATGCTGAATGCT+TGG 0.483188 4:-22045803 MsaT019029.1:CDS
ACCTTGAGTTTCATAAGTAT+GGG 0.487157 4:+22044740 None:intergenic
TCTATCGATGCAACTAGAAA+AGG 0.490560 4:-22044597 MsaT019029.1:CDS
GTTGTTCATGGTTTGAGTGT+CGG 0.491939 4:+22045852 None:intergenic
GAGTCCATTCAAGCTATTAG+TGG 0.503625 4:+22044069 None:intergenic
ATCATGCCCATGCATTGAAA+AGG 0.512630 4:+22044036 None:intergenic
ATCATGCCCATGCATTGAAA+AGG 0.512630 4:+22044124 None:intergenic
AAGAGTTGGAATATTTGCAC+TGG 0.522066 4:-22044361 MsaT019029.1:CDS
GAGTTGTGTCTTCAGAGGAA+TGG 0.522151 4:-22044800 MsaT019029.1:CDS
AAGCTGTTCAAACTGCTAGC+AGG 0.527660 4:-22045699 MsaT019029.1:intron
TGAATGGTTTGGAGGTGCAA+AGG 0.527707 4:-22044295 MsaT019029.1:CDS
AATGGTTTATAAATCTTGCA+AGG 0.529739 4:+22044571 None:intergenic
ATAAGTATGGGATCTACACT+TGG 0.529929 4:+22044752 None:intergenic
ACAAGAAAATGGAATGTCAT+GGG 0.544715 4:-22044390 MsaT019029.1:CDS
TTCCTTGGAACAAAATCTCG+TGG 0.560409 4:-22044237 MsaT019029.1:CDS
GACAAGAAAATGGAATGTCA+TGG 0.568296 4:-22044391 MsaT019029.1:CDS
ATGGGCATGATGTTGACTGC+TGG 0.586934 4:-22044024 MsaT019029.1:CDS
CTAATAGCTTGAATGGACTC+AGG 0.596311 4:-22044066 MsaT019029.1:CDS
AACATTTGAGCGCACTACGC+AGG 0.597700 4:+22044265 None:intergenic
AGTCCATTCAAGCTATTAGT+GGG 0.608535 4:+22044070 None:intergenic
TCCCATACTTATGAAACTCA+AGG 0.623281 4:-22044741 MsaT019029.1:intron
CTTGAATGGACTCAGGTCTG+AGG 0.627318 4:-22044059 MsaT019029.1:CDS
GACAAGAGTTGTGTCTTCAG+AGG 0.640474 4:-22044805 MsaT019029.1:CDS
CAAGAAAATGGAATGTCATG+GGG 0.642373 4:-22044389 MsaT019029.1:CDS
AAGAAAATGGAATGTCATGG+GGG 0.698806 4:-22044388 MsaT019029.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 22044006 22045911 22044006 ID=MsaG019029
Chr4 mRNA 22044006 22045911 22044006 ID=MsaT019029.1;Parent=MsaG019029
Chr4 exon 22044006 22044146 22044006 ID=MsaT019029.1.exon5;Parent=MsaT019029.1
Chr4 CDS 22044006 22044146 22044006 ID=cds.MsaT019029.1;Parent=MsaT019029.1
Chr4 exon 22044230 22044428 22044230 ID=MsaT019029.1.exon4;Parent=MsaT019029.1
Chr4 CDS 22044230 22044428 22044230 ID=cds.MsaT019029.1;Parent=MsaT019029.1
Chr4 exon 22044567 22044660 22044567 ID=MsaT019029.1.exon3;Parent=MsaT019029.1
Chr4 CDS 22044567 22044660 22044567 ID=cds.MsaT019029.1;Parent=MsaT019029.1
Chr4 exon 22044742 22044833 22044742 ID=MsaT019029.1.exon2;Parent=MsaT019029.1
Chr4 CDS 22044742 22044833 22044742 ID=cds.MsaT019029.1;Parent=MsaT019029.1
Chr4 exon 22045700 22045911 22045700 ID=MsaT019029.1.exon1;Parent=MsaT019029.1
Chr4 CDS 22045700 22045911 22045700 ID=cds.MsaT019029.1;Parent=MsaT019029.1
Gene Sequence

>MsaT019029.1

ATGCAAGTTTTATGGGGAATTTTGGACTTTGAGCAGCCCGACACTCAAACCATGAACAACATTGTTATATCTACAGTTGAACTTATTTATTGTTATGCTGAATGCTTGGCCCTCCATGTAAAAGATTCAGGAGTGCACTGTGTAGCACCTGCTGTTGTATTACTGAAGAAACTTCTTTTTTCGTCTGATGAAGCTGTTCAAACTGCTAGCAGGAATACGACAAGAGTTGTGTCTTCAGAGGAATGGAAAGAAGTAATATCATGCAAAGAAGCCAAGTGTAGATCCCATACTTATGAAACTCAAGGTCTTAAGGATGCATTTATCATTTCTCTTAATGCGTCTGAATCTATCGATGCAACTAGAAAAGGAAGCCTTGCAAGATTTATAAACCATTCTTGTCAACCAAATTGTGAGACAAGAAAATGGAATGTCATGGGGGAAATAAGAGTTGGAATATTTGCACTGGAAGATATTCCTATTGGAACTGAACTGGCATATGACTATAATTTTGAATGGTTTGGAGGTGCAAAGGTTATTTGCCTGCGTAGTGCGCTCAAATGTTCTGAATTCCTTGGAACAAAATCTCGTGGTTTTCAACCTTTTCAATGCATGGGCATGATGTTGACTGTTGTAGACCATGAGTTTTTAAGACCCACTAATAGCTTGAATGGACTCAGGTCTGAGGCCTTTTCAATGCATGGGCATGATGTTGACTGCTGGAAATTGAATGATACTTGA

Protein sequence

>MsaT019029.1

MQVLWGILDFEQPDTQTMNNIVISTVELIYCYAECLALHVKDSGVHCVAPAVVLLKKLLFSSDEAVQTASRNTTRVVSSEEWKEVISCKEAKCRSHTYETQGLKDAFIISLNASESIDATRKGSLARFINHSCQPNCETRKWNVMGEIRVGIFALEDIPIGTELAYDYNFEWFGGAKVICLRSALKCSEFLGTKSRGFQPFQCMGMMLTVVDHEFLRPTNSLNGLRSEAFSMHGHDVDCWKLNDT*