AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019113


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019113 MtrunA17_Chr4g0011841 99.548 221 1 0 1 221 1 221 9.76e-169 463
MsaG019113 MtrunA17_Chr4g0056521 71.770 209 51 6 18 221 13 218 1.52e-93 273
MsaG019113 MtrunA17_Chr4g0063931 53.368 193 64 4 20 206 11 183 3.05e-60 187
MsaG019113 MtrunA17_Chr2g0322221 52.041 196 83 5 19 211 8 195 4.01e-57 179
MsaG019113 MtrunA17_Chr4g0025361 54.601 163 61 3 22 184 8 157 2.97e-51 164
MsaG019113 MtrunA17_Chr5g0413381 50.000 158 71 2 23 179 12 162 1.23e-46 152
MsaG019113 MtrunA17_Chr5g0419871 46.875 160 65 5 21 178 14 155 2.32e-35 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019113 AT2G45190.1 67.431 218 49 6 19 221 19 229 1.45e-96 281
MsaG019113 AT4G00180.1 62.661 233 59 10 8 219 13 238 9.63e-81 241
MsaG019113 AT4G00180.2 61.722 209 57 9 32 219 1 207 4.42e-68 208
MsaG019113 AT2G45190.2 63.372 172 41 6 65 221 1 165 7.28e-66 201
MsaG019113 AT2G26580.2 57.485 167 49 5 22 186 8 154 1.74e-56 177
MsaG019113 AT2G26580.1 57.485 167 49 5 22 186 8 154 1.74e-56 177
MsaG019113 AT1G08465.1 56.322 174 52 5 22 188 7 163 1.42e-55 175
MsaG019113 AT1G23420.1 46.584 161 84 2 24 182 20 180 8.17e-40 137
MsaG019113 AT1G23420.2 46.584 161 84 2 24 182 51 211 4.45e-39 135
MsaG019113 AT1G69180.1 39.490 157 77 2 22 178 18 156 9.86e-31 112

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTAGCAGGTGGAGGTTT+AGG 0.195251 4:-23073692 None:intergenic
ATCATCAAATCATTTGGATT+TGG 0.277959 4:-23073635 None:intergenic
CACTTTCCACACATTCATTT+CGG 0.297543 4:+23075862 MsaT019113.1:CDS
CTTCAACCCTCAAACTCTTA+TGG 0.305185 4:+23073204 MsaT019113.1:CDS
AGAGTTGTTGCTGTTGTTGC+TGG 0.315337 4:-23072884 None:intergenic
TATTAGCAGGTGGAGGTTTA+GGG 0.330394 4:-23073691 None:intergenic
GCCTCTCTGTGGCTTATATC+AGG 0.334060 4:-23075630 None:intergenic
TTCTTCACGGGTTGATGATC+AGG 0.349751 4:-23075892 None:intergenic
AACAAACACACTTCTTTAGT+AGG 0.360387 4:-23076139 None:intergenic
GTGTTCATCATCAAATCATT+TGG 0.376681 4:-23073641 None:intergenic
TTACTTGATGAAGCGGTTGT+AGG 0.396976 4:-23074696 None:intergenic
TGTCCAAGGTGAAGTTGATT+TGG 0.407277 4:-23073175 None:intergenic
TGTTGCTGTTGTTGCTGGTC+CGG 0.423782 4:-23072879 None:intergenic
GAGGACGTGCTTATGAAAGA+TGG 0.432558 4:+23076084 MsaT019113.1:CDS
TTCATCATCATGTTTGTTGA+AGG 0.455442 4:-23073605 None:intergenic
AATTTCTTACGTCTATTAGC+AGG 0.457462 4:-23073704 None:intergenic
GAGATCCAACGTATCAAAGC+TGG 0.478771 4:+23075603 MsaT019113.1:CDS
GAAGAAAGCTAATGTTCGTC+AGG 0.494623 4:+23075909 MsaT019113.1:CDS
CGAACATTAGCTTTCTTCAC+GGG 0.494923 4:-23075904 None:intergenic
ACAAACACACTTCTTTAGTA+GGG 0.496640 4:-23076138 None:intergenic
ACTCATACCATAAGAGTTTG+AGG 0.497892 4:-23073211 None:intergenic
GGAACTCTCTGTCTCTTCTC+CGG 0.501243 4:-23074668 None:intergenic
CGAAATGAATGTGTGGAAAG+TGG 0.502844 4:-23075861 None:intergenic
TTGTTGCTGGTCCGGTGAAA+AGG 0.508490 4:-23072871 None:intergenic
AGGTGAAGTTGATTTGGAGC+AGG 0.517398 4:-23073169 None:intergenic
ACAGCAACAACTCTCTCCTT+CGG 0.518591 4:+23072893 MsaT019113.1:CDS
TGCAATGGACATAACAAAGC+TGG 0.526911 4:-23072917 None:intergenic
CTCATACCATAAGAGTTTGA+GGG 0.529909 4:-23073210 None:intergenic
ATTAAACTTACTTGATGAAG+CGG 0.531165 4:-23074703 None:intergenic
ACGAACATTAGCTTTCTTCA+CGG 0.533864 4:-23075905 None:intergenic
TTACGTCTATTAGCAGGTGG+AGG 0.545636 4:-23073698 None:intergenic
CTGGTCCGGTGAAAAGGAAG+TGG 0.549720 4:-23072865 None:intergenic
AGGACGTGCTTATGAAAGAT+GGG 0.553995 4:+23076085 MsaT019113.1:CDS
TTTCATTGTGTAGGATTCAG+AGG 0.560695 4:+23076065 MsaT019113.1:intron
ATGAAGCGGTTGTAGGCAGA+TGG 0.560728 4:-23074689 None:intergenic
TTCTTACGTCTATTAGCAGG+TGG 0.566691 4:-23073701 None:intergenic
GATTCCCAGCTTTGATACGT+TGG 0.580008 4:-23075608 None:intergenic
GAAGTTGATTTGGAGCAGGA+AGG 0.583401 4:-23073165 None:intergenic
AAAGGAAGTGGATGATGAAG+AGG 0.587043 4:-23072853 None:intergenic
GCTCCAAATCAACTTCACCT+TGG 0.591195 4:+23073172 MsaT019113.1:CDS
AGATCCAACGTATCAAAGCT+GGG 0.600203 4:+23075604 MsaT019113.1:CDS
GAAATGAATGTGTGGAAAGT+GGG 0.615090 4:-23075860 None:intergenic
ATGATGAACACAATGAGAGG+AGG 0.619560 4:+23073653 MsaT019113.1:CDS
CACGCATGTTAACTGAGAGA+AGG 0.621310 4:-23073135 None:intergenic
CATGTTAACTGAGAGAAGGT+TGG 0.622869 4:-23073131 None:intergenic
TGATGAACACAATGAGAGGA+GGG 0.625320 4:+23073654 MsaT019113.1:CDS
ACATAACAAAGCTGGTCCGA+AGG 0.642303 4:-23072909 None:intergenic
GGTTGAAGTAAGTGTGTCCA+AGG 0.648511 4:-23073189 None:intergenic
GTCTTGAACAAACTTGTGCA+AGG 0.649953 4:-23073082 None:intergenic
CAGTGTCACAGAAATTGCAA+TGG 0.650774 4:-23072932 None:intergenic
TTTCTGTGACACTGTTCTCG+CGG 0.670363 4:+23072941 MsaT019113.1:CDS
CTGCACTAAAAGCCTCTCTG+TGG 0.673003 4:-23075641 None:intergenic
AAGACTGTGACAGTGAGATG+TGG 0.685505 4:+23073100 MsaT019113.1:CDS
TTGATGATGAACACAATGAG+AGG 0.686722 4:+23073650 MsaT019113.1:CDS
TCCTGATATAAGCCACAGAG+AGG 0.691291 4:+23075629 MsaT019113.1:CDS
ATAAGTCCGAAATGAATGTG+TGG 0.723401 4:-23075868 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 23072849 23076146 23072849 ID=MsaG019113
Chr4 mRNA 23072849 23076146 23072849 ID=MsaT019113.1;Parent=MsaG019113
Chr4 exon 23072849 23072962 23072849 ID=MsaT019113.1.exon1;Parent=MsaT019113.1
Chr4 CDS 23072849 23072962 23072849 ID=cds.MsaT019113.1;Parent=MsaT019113.1
Chr4 exon 23073073 23073225 23073073 ID=MsaT019113.1.exon2;Parent=MsaT019113.1
Chr4 CDS 23073073 23073225 23073073 ID=cds.MsaT019113.1;Parent=MsaT019113.1
Chr4 exon 23073587 23073716 23073587 ID=MsaT019113.1.exon3;Parent=MsaT019113.1
Chr4 CDS 23073587 23073716 23073587 ID=cds.MsaT019113.1;Parent=MsaT019113.1
Chr4 exon 23074666 23074714 23074666 ID=MsaT019113.1.exon4;Parent=MsaT019113.1
Chr4 CDS 23074666 23074714 23074666 ID=cds.MsaT019113.1;Parent=MsaT019113.1
Chr4 exon 23075599 23075674 23075599 ID=MsaT019113.1.exon5;Parent=MsaT019113.1
Chr4 CDS 23075599 23075674 23075599 ID=cds.MsaT019113.1;Parent=MsaT019113.1
Chr4 exon 23075856 23075930 23075856 ID=MsaT019113.1.exon6;Parent=MsaT019113.1
Chr4 CDS 23075856 23075930 23075856 ID=cds.MsaT019113.1;Parent=MsaT019113.1
Chr4 exon 23076078 23076146 23076078 ID=MsaT019113.1.exon7;Parent=MsaT019113.1
Chr4 CDS 23076078 23076146 23076078 ID=cds.MsaT019113.1;Parent=MsaT019113.1
Gene Sequence

>MsaT019113.1

ATGTCCTCTTCATCATCCACTTCCTTTTCACCGGACCAGCAACAACAGCAACAACTCTCTCCTTCGGACCAGCTTTGTTATGTCCATTGCAATTTCTGTGACACTGTTCTCGCGGTGAGTGTTCCTTGCACAAGTTTGTTCAAGACTGTGACAGTGAGATGTGGTCATTGCACCAACCTTCTCTCAGTTAACATGCGTGCTTTGCTCCTTCCTGCTCCAAATCAACTTCACCTTGGACACACTTACTTCAACCCTCAAACTCTTATGGAGGAGATTCGAAATGCACCTTCAACAAACATGATGATGAATCAGATTCCAAATCCAAATGATTTGATGATGAACACAATGAGAGGAGGGCATCATGAAGAAATCCCTAAACCTCCACCTGCTAATAGACCTCCGGAGAAGAGACAGAGAGTTCCATCTGCCTACAACCGCTTCATCAAGGATGAGATCCAACGTATCAAAGCTGGGAATCCTGATATAAGCCACAGAGAGGCTTTTAGTGCAGCTGCAAAGAATTGGGCCCACTTTCCACACATTCATTTCGGACTTATGCCTGATCATCAACCCGTGAAGAAAGCTAATGTTCGTCAGGATTCAGAGGACGTGCTTATGAAAGATGGGTTTTTCACACAAGCAAATGTAGGTGTCTCTCCCTACTAA

Protein sequence

>MsaT019113.1

MSSSSSTSFSPDQQQQQQLSPSDQLCYVHCNFCDTVLAVSVPCTSLFKTVTVRCGHCTNLLSVNMRALLLPAPNQLHLGHTYFNPQTLMEEIRNAPSTNMMMNQIPNPNDLMMNTMRGGHHEEIPKPPPANRPPEKRQRVPSAYNRFIKDEIQRIKAGNPDISHREAFSAAAKNWAHFPHIHFGLMPDHQPVKKANVRQDSEDVLMKDGFFTQANVGVSPY*