AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019147

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019147 MtrunA17_Chr4g0012511 93.472 1011 66 0 1 1011 1 1011 0.0 1904
MsaG019147 MtrunA17_Chr6g0479161 67.023 1028 303 13 5 1011 161 1173 0.0 1344
MsaG019147 MtrunA17_Chr3g0113591 34.036 664 299 26 33 606 60 674 2.81e-81 283
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019147 AT1G10170.1 59.547 1016 354 13 25 1011 201 1188 0.0 1201
MsaG019147 AT1G10170.3 59.547 1016 354 13 25 1011 201 1188 0.0 1201
MsaG019147 AT1G10170.2 59.547 1016 354 13 25 1011 129 1116 0.0 1199
MsaG019147 AT1G10170.4 59.585 1012 352 13 25 1007 201 1184 0.0 1196
MsaG019147 AT5G05660.1 38.477 499 238 18 33 505 70 525 6.55e-79 276
MsaG019147 AT5G05660.1 28.671 429 210 19 148 491 306 723 2.97e-17 87.8

Find 282 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGAGGCGTGATAGTAAA+TGG 0.186116 4:+23747499 MsaT019147.1:CDS
GTTGCATTTAGAATATTATT+TGG 0.196154 4:-23746613 None:intergenic
AATCTAGATTCTTTCCAATT+TGG 0.203871 4:+23747438 MsaT019147.1:CDS
ATCACGCGTGCTAGGGATTA+AGG 0.206878 4:+23747737 MsaT019147.1:CDS
AACTAGTGCATGACCATATA+GGG 0.252374 4:-23745601 None:intergenic
GCGCTAAGTTGGAGCGTAAA+AGG 0.254968 4:+23747382 MsaT019147.1:CDS
TGAACAACTATAAATTTCTT+TGG 0.257240 4:-23747690 None:intergenic
GTCGGTAGTTGAGGGTGGTC+TGG 0.279464 4:-23745483 None:intergenic
TTGAGCCACACCGACACTTC+TGG 0.282122 4:-23746462 None:intergenic
GGATCATGGAAGTGTTTATC+AGG 0.282695 4:+23747980 MsaT019147.1:CDS
TCAACTTCTGCAAGAGATTC+AGG 0.293641 4:+23745517 MsaT019147.1:CDS
CAAATATTCAATCATCTATA+TGG 0.300265 4:+23748195 MsaT019147.1:CDS
AGCGCCAATCAATGTTAGTT+TGG 0.301750 4:-23745685 None:intergenic
CTTACAACACTCCACACTTT+TGG 0.307644 4:-23746488 None:intergenic
CAGCTTGTAGAAAAGATTCT+TGG 0.321054 4:+23748144 MsaT019147.1:CDS
CAACAGAGTTCTCACCAAAT+TGG 0.337343 4:-23747452 None:intergenic
GCTTCCAACAGAGGGAAATT+AGG 0.340655 4:+23745432 MsaT019147.1:CDS
ACTTGTGAACATGTAGGAAT+TGG 0.341644 4:-23746352 None:intergenic
TCCGCAGATCTTCCCCTATA+TGG 0.343713 4:+23745588 MsaT019147.1:CDS
GTGGCAAGTCTCTTCCTTGC+GGG 0.343940 4:+23746377 MsaT019147.1:CDS
AATCTCCATGATGACAACTA+TGG 0.352235 4:-23746873 None:intergenic
GACAGGTTATACGTGGAAAA+AGG 0.357309 4:+23748106 MsaT019147.1:CDS
AATTGGAAAGAATCTAGATT+TGG 0.361226 4:-23747435 None:intergenic
TTTGCATCTCTTGGTAAGTC+TGG 0.366649 4:-23747840 None:intergenic
AAGGACCGAGATGACAAATT+TGG 0.372071 4:-23746048 None:intergenic
CATGCATTCGACGGTGCCTT+TGG 0.373867 4:-23745549 None:intergenic
GCGAATCCACATCTTCTATC+TGG 0.375373 4:-23747174 None:intergenic
TTTATGATGTAGTAGATGAT+TGG 0.375641 4:+23748423 MsaT019147.1:CDS
CTTCCCAAACTAACATTGAT+TGG 0.396238 4:+23745681 MsaT019147.1:CDS
GCAGAAGTACCTGATGGAAT+TGG 0.397861 4:-23745741 None:intergenic
AATTTGGAGGTTATAATTTG+TGG 0.399308 4:+23746118 MsaT019147.1:CDS
AGTGCATTGGTGTTGAGATT+TGG 0.406762 4:+23747867 MsaT019147.1:CDS
GTTCGAATACCTTGCACAAC+AGG 0.411113 4:-23747069 None:intergenic
GCGCCAATCAATGTTAGTTT+GGG 0.412220 4:-23745684 None:intergenic
CAACTAGTGCATGACCATAT+AGG 0.414791 4:-23745602 None:intergenic
ATTTGGAGGTTATAATTTGT+GGG 0.417423 4:+23746119 MsaT019147.1:CDS
TCTCATTGCAGTTGCAGCTC+GGG 0.421489 4:-23747952 None:intergenic
TTGGTGGGCATGTAGTTCTT+AGG 0.423032 4:+23746932 MsaT019147.1:CDS
CATGCTTCTGCGGGAAAACT+AGG 0.423070 4:+23746299 MsaT019147.1:CDS
TGCAATAAATGAAGCCGGTC+GGG 0.423345 4:+23747665 MsaT019147.1:CDS
TCGGTAGTTGAGGGTGGTCT+GGG 0.429591 4:-23745482 None:intergenic
ATTGAGGCGTGATAGTAAAT+GGG 0.433029 4:+23747500 MsaT019147.1:CDS
TGAGCCACACCGACACTTCT+GGG 0.440846 4:-23746461 None:intergenic
GTGGTGAGTGCGAACTTGTT+TGG 0.444024 4:+23747889 MsaT019147.1:CDS
ACAAATGACCGGGGAGGAGC+AGG 0.444186 4:+23748053 MsaT019147.1:CDS
AGCATGTAAACCGCACTGTT+TGG 0.444304 4:-23747007 None:intergenic
TGAGGATGATTGCTGATAGA+TGG 0.445052 4:+23747637 MsaT019147.1:CDS
ATGTATTGAGACCTGTCATC+CGG 0.445537 4:+23746234 MsaT019147.1:CDS
TTACTACTTCATCAAATCCT+TGG 0.445814 4:+23748234 MsaT019147.1:CDS
TTAGTACTTGGCAAGGGTAA+AGG 0.448003 4:+23747552 MsaT019147.1:CDS
TTGTGGGGACATGGCTGTAA+AGG 0.448483 4:+23746135 MsaT019147.1:CDS
GGACACTCAACACAACTTCC+CGG 0.450132 4:-23746253 None:intergenic
GCAATGACCACTGTGACAAT+TGG 0.451305 4:-23746707 None:intergenic
TTCTGCGGGAAAACTAGGTT+GGG 0.453846 4:+23746304 MsaT019147.1:CDS
GCTACAGATGTTCCTGTGTT+CGG 0.457674 4:-23748020 None:intergenic
AACATTGATTGGCGCTGCCC+TGG 0.458005 4:+23745692 MsaT019147.1:CDS
GCACTGATATTTGCATCTCT+TGG 0.459202 4:-23747849 None:intergenic
CTATCTGGACATGGAGATGA+AGG 0.461783 4:-23747159 None:intergenic
AGTTGCACAGATTGACAGCC+AGG 0.467615 4:-23745710 None:intergenic
AATTACTTGCTGATTTATTG+AGG 0.467758 4:+23747484 MsaT019147.1:CDS
CCCACTTGGTTGTACAATTG+AGG 0.468327 4:-23745640 None:intergenic
TGTGTCTTGCAATGTCATCC+TGG 0.469353 4:+23745875 MsaT019147.1:CDS
TTATGATGTAGTAGATGATT+GGG 0.470146 4:+23748424 MsaT019147.1:CDS
ATCTCTCTTATCCTTTAGCA+TGG 0.474075 4:-23747610 None:intergenic
AGAGCTCCATCACGCGTGCT+AGG 0.475286 4:+23747729 MsaT019147.1:CDS
CGCTAAGTTGGAGCGTAAAA+GGG 0.476238 4:+23747383 MsaT019147.1:CDS
GTCCACATGTAATAAGAGAT+TGG 0.478835 4:-23745982 None:intergenic
CCTTGTGATGCTGGTGGTAG+TGG 0.479025 4:+23747240 MsaT019147.1:CDS
TCACGCGTGCTAGGGATTAA+GGG 0.479156 4:+23747738 MsaT019147.1:CDS
AGCTTGTAGAAAAGATTCTT+GGG 0.479947 4:+23748145 MsaT019147.1:CDS
CTTGTGAACATGTAGGAATT+GGG 0.482207 4:-23746351 None:intergenic
GATTCCTAATTTCCCTCTGT+TGG 0.482364 4:-23745436 None:intergenic
ATCCTCGTCGTCGTCAGCAA+TGG 0.484767 4:+23745456 MsaT019147.1:CDS
GCAGAAGTTGAGGTAATAAC+CGG 0.484843 4:-23745505 None:intergenic
CTTAACATTGGTTGGTAACA+AGG 0.487573 4:-23746275 None:intergenic
TTCTCATTGCAGTTGCAGCT+CGG 0.489289 4:-23747953 None:intergenic
GGTGAATACAAAGCAGAAGC+TGG 0.489764 4:+23746157 MsaT019147.1:CDS
GGTTATAATTTGTGGGGACA+TGG 0.493536 4:+23746126 MsaT019147.1:CDS
CTTCTGCGGGAAAACTAGGT+TGG 0.494125 4:+23746303 MsaT019147.1:CDS
TAAAAGTCTGATGGAGGGTC+GGG 0.495631 4:-23745779 None:intergenic
CTTCAACCATTGGATGCAAA+TGG 0.497476 4:+23747312 MsaT019147.1:CDS
TGGAAGAGCATTTCACACTT+TGG 0.497940 4:+23748397 MsaT019147.1:CDS
TGTAAAATGACATGCCATGA+TGG 0.499854 4:+23746409 MsaT019147.1:CDS
TTACTTGTGAAAAGCCTTGT+GGG 0.502098 4:+23746533 MsaT019147.1:CDS
GTACCACATGGCAATGGAGG+AGG 0.504974 4:-23746790 None:intergenic
AACACAACTTCCCGGATGAC+AGG 0.506370 4:-23746245 None:intergenic
AAGCATGTCTTAACATTGGT+TGG 0.508637 4:-23746283 None:intergenic
CTTATATCCCTTGAGCTACA+TGG 0.509314 4:-23746961 None:intergenic
GCATTGGTGTTGAGATTTGG+TGG 0.509380 4:+23747870 MsaT019147.1:CDS
TAATGATGAGTGCGCTAAGT+TGG 0.511574 4:+23747371 MsaT019147.1:CDS
ATTTAATTCTTTAGTAGACA+TGG 0.511596 4:+23747794 MsaT019147.1:CDS
TCTTGATTTAAGACAGTCCA+AGG 0.511833 4:-23748251 None:intergenic
TTCCACGTATAACCTGTCAA+TGG 0.512019 4:-23748101 None:intergenic
GGGAAGCCTCTTCAGAAGAC+GGG 0.514646 4:+23745833 MsaT019147.1:CDS
CATCTTCAGTTACAAATGAC+CGG 0.514836 4:+23748042 MsaT019147.1:CDS
TGGACTGTCTTAAATCAAGA+AGG 0.515374 4:+23748254 MsaT019147.1:CDS
ATATTCTAAATGCAACGGAT+TGG 0.516278 4:+23746620 MsaT019147.1:CDS
ATTGCAGTTGCAGCTCGGGC+AGG 0.516373 4:-23747948 None:intergenic
TAGCTTCTTTCCACAAGGAA+TGG 0.517251 4:-23746659 None:intergenic
AAATTTCTTTGGCTCCCGAC+CGG 0.518690 4:-23747679 None:intergenic
TCACTATCCCTGCTCCTCCC+CGG 0.519618 4:-23748061 None:intergenic
TAATAACCGGTCGGTAGTTG+AGG 0.521137 4:-23745492 None:intergenic
GTGGAAAGAAGCTACGTTGT+GGG 0.521463 4:+23746668 MsaT019147.1:CDS
ACAAACGTCCCTTGTGATGC+TGG 0.525383 4:+23747231 MsaT019147.1:CDS
ATTCATGTGGAGAACCTTGT+GGG 0.526413 4:+23745813 MsaT019147.1:CDS
CCAACACATTCTCTTGCTAC+AGG 0.527679 4:-23746913 None:intergenic
GAGCTCCATCACGCGTGCTA+GGG 0.531617 4:+23747730 MsaT019147.1:CDS
GCTGGATGGCCACAAGGTTG+AGG 0.532402 4:-23746847 None:intergenic
ATCTCCATGATGACAACTAT+GGG 0.533494 4:-23746872 None:intergenic
ATATTATTTGGATTAGAGAG+TGG 0.534885 4:-23746601 None:intergenic
TGTCACCAAATTTGTCATCT+CGG 0.535179 4:+23746043 MsaT019147.1:CDS
GCATCCCATAGTTGTCATCA+TGG 0.536732 4:+23746868 MsaT019147.1:CDS
TGTGGGGACATGGCTGTAAA+GGG 0.537176 4:+23746136 MsaT019147.1:CDS
GCTTGTGCCACTTCAACCAT+TGG 0.537486 4:+23747302 MsaT019147.1:CDS
GCAATGAGAAGGTTGGATCA+TGG 0.538179 4:+23747966 MsaT019147.1:CDS
ACATGCAAGTTTGTTTCCAC+AGG 0.545473 4:-23746200 None:intergenic
CCTCCTCGTTTGTGCCCTTG+TGG 0.546878 4:+23745926 MsaT019147.1:CDS
ACAAGGGCACAAACGAGGAG+GGG 0.546918 4:-23745924 None:intergenic
CAAGGTTGAGGAACTGAACA+TGG 0.547360 4:-23746835 None:intergenic
AATGCTTTGCAAGGAGGACA+TGG 0.548434 4:-23745899 None:intergenic
CACAAGGGCACAAACGAGGA+GGG 0.548704 4:-23745925 None:intergenic
TCACCTCATCCAATTCCATC+AGG 0.548712 4:+23745732 MsaT019147.1:CDS
TGATGACAACTATGGGATGC+TGG 0.549773 4:-23746865 None:intergenic
AAGAATTAAATGCAGGAGGA+AGG 0.551221 4:-23747782 None:intergenic
AAACTAGGTTGGGCGAGGAA+CGG 0.552198 4:+23746314 MsaT019147.1:CDS
TGTACAATTGAGGTGAAAGA+TGG 0.554002 4:-23745630 None:intergenic
CATCTCTGTTCCATTCCTTG+TGG 0.554147 4:+23746649 MsaT019147.1:CDS
CCACATCTTCTATCTGGACA+TGG 0.554367 4:-23747168 None:intergenic
GTTTCCACAGGTAGAACCAC+AGG 0.555787 4:-23746188 None:intergenic
ATCTTCAGTTACAAATGACC+GGG 0.557940 4:+23748043 MsaT019147.1:CDS
TGGGAAGCCTCTTCAGAAGA+CGG 0.559048 4:+23745832 MsaT019147.1:CDS
GTTAGTTTGGGAAGATGAAG+TGG 0.560036 4:-23745672 None:intergenic
TCTACTAAAGAATTAAATGC+AGG 0.561015 4:-23747789 None:intergenic
ATGTTAAGACATGCTTCTGC+GGG 0.561286 4:+23746290 MsaT019147.1:CDS
GTATGTCGGCAGTCTCTCCT+CGG 0.561475 4:-23747117 None:intergenic
TGTTCAGTTCCTCAACCTTG+TGG 0.562802 4:+23746838 MsaT019147.1:CDS
ATAAAAGTCTGATGGAGGGT+CGG 0.563295 4:-23745780 None:intergenic
CAAGGTATTCGAACTTCATG+TGG 0.563446 4:+23747078 MsaT019147.1:CDS
GCGTCTACTGCAACACGACA+TGG 0.565166 4:-23746076 None:intergenic
AATGCAGGAGGAAGGAGAGA+AGG 0.565516 4:-23747774 None:intergenic
AAGGAGAACAATTCCCATCA+TGG 0.565758 4:-23746423 None:intergenic
TTTAATTCTTTAGTAGACAT+GGG 0.565937 4:+23747795 MsaT019147.1:CDS
TTCTGAAGAGGCTTCCCACA+AGG 0.566616 4:-23745827 None:intergenic
TTTGCATCCAATGGTTGAAG+TGG 0.566617 4:-23747309 None:intergenic
CATGGAGATGAAGGGTGACA+AGG 0.567442 4:-23747150 None:intergenic
GGAACATACCATGTAGCTCA+AGG 0.567840 4:+23746953 MsaT019147.1:CDS
TGTATTGAGACCTGTCATCC+GGG 0.568271 4:+23746235 MsaT019147.1:CDS
TTAATCCCTAGCACGCGTGA+TGG 0.571628 4:-23747735 None:intergenic
AGATGCAAATATCAGTGCAT+TGG 0.572264 4:+23747854 MsaT019147.1:CDS
AAATAATATTCTAAATGCAA+CGG 0.574868 4:+23746615 MsaT019147.1:CDS
GGAAAAGAGACGACAAATCG+AGG 0.575381 4:-23747819 None:intergenic
CCATGTCCAGATAGAAGATG+TGG 0.575500 4:+23747168 MsaT019147.1:CDS
TGAATCTCTTGCAGAAGTTG+AGG 0.576515 4:-23745515 None:intergenic
GGAGGAGCAGGGATAGTGAA+AGG 0.577711 4:+23748065 MsaT019147.1:CDS
AATGTTAAGACATGCTTCTG+CGG 0.577777 4:+23746289 MsaT019147.1:CDS
GTTTGTTGTTAAACGACCAC+AGG 0.578946 4:-23747214 None:intergenic
GGAGGGGCAAATGCTTTGCA+AGG 0.579252 4:-23745908 None:intergenic
CGGGAAAACTAGGTTGGGCG+AGG 0.580444 4:+23746309 MsaT019147.1:CDS
TGTGGCAAGTCTCTTCCTTG+CGG 0.580803 4:+23746376 MsaT019147.1:CDS
CCTACATGTTCACAAGTATG+TGG 0.583101 4:+23746358 MsaT019147.1:CDS
TTGCAAGGAGGACATGGACC+AGG 0.583371 4:-23745893 None:intergenic
CCACATACTTGTGAACATGT+AGG 0.583521 4:-23746358 None:intergenic
TGCAACTGCAATGAGAAGGT+TGG 0.584397 4:+23747959 MsaT019147.1:CDS
CAACGTAGCTTCTTTCCACA+AGG 0.584502 4:-23746664 None:intergenic
GGTGCGCTGACACCATTGAC+AGG 0.584564 4:+23748089 MsaT019147.1:CDS
CCACTACCACCAGCATCACA+AGG 0.585195 4:-23747240 None:intergenic
TTTGGAGGTTATAATTTGTG+GGG 0.585414 4:+23746120 MsaT019147.1:CDS
GCAGAAGCATGTCTTAACAT+TGG 0.586408 4:-23746287 None:intergenic
TATCTCACGCCGCATTCATG+TGG 0.586486 4:+23745800 MsaT019147.1:CDS
AGGAGGAAGGAGAGAAGGTA+CGG 0.588114 4:-23747769 None:intergenic
TTAGCATGGGACAGAAAACA+TGG 0.588948 4:-23747596 None:intergenic
TCTTGCTACAGGAGCCACAC+AGG 0.591633 4:-23746902 None:intergenic
TTTACTTGTGAAAAGCCTTG+TGG 0.592462 4:+23746532 MsaT019147.1:CDS
AGTGGGTGCACGAGCCCACT+TGG 0.592515 4:-23745654 None:intergenic
TCTCTCTTATCCTTTAGCAT+GGG 0.592888 4:-23747609 None:intergenic
AGATTCTTGGGATGATCAAG+AGG 0.593703 4:+23748157 MsaT019147.1:CDS
GGCAATCATGTGGCGCTCCG+AGG 0.596714 4:+23747100 MsaT019147.1:CDS
GACCAATCTCTTATTACATG+TGG 0.599318 4:+23745980 MsaT019147.1:CDS
AATAACCGGTCGGTAGTTGA+GGG 0.601648 4:-23745491 None:intergenic
GTAAAATGACATGCCATGAT+GGG 0.603535 4:+23746410 MsaT019147.1:CDS
TTGCAATAAATGAAGCCGGT+CGG 0.603586 4:+23747664 MsaT019147.1:CDS
TGGCTCAACTTCCAAAAGTG+TGG 0.603861 4:+23746477 MsaT019147.1:CDS
GACCACCCTCAACTACCGAC+CGG 0.603996 4:+23745486 MsaT019147.1:CDS
ACAAAAGCAGAAGTACCTGA+TGG 0.605405 4:-23745747 None:intergenic
GTCACAATCACTTGTACCTG+TGG 0.605414 4:+23747198 MsaT019147.1:CDS
ACTGATTTGACATGTGCTTG+TGG 0.605706 4:+23746754 MsaT019147.1:CDS
CATTCATGTGGAGAACCTTG+TGG 0.605707 4:+23745812 MsaT019147.1:CDS
GATTCTTGGGATGATCAAGA+GGG 0.606933 4:+23748158 MsaT019147.1:CDS
GTGAGATAAAAGTCTGATGG+AGG 0.608948 4:-23745785 None:intergenic
TGGGAGACAACTTGTGAATG+AGG 0.610421 4:+23748443 MsaT019147.1:CDS
AAGCTTGCAATAAATGAAGC+CGG 0.611009 4:+23747660 MsaT019147.1:CDS
ACCATATAGGGGAAGATCTG+CGG 0.611313 4:-23745589 None:intergenic
AAGTCAGAATCTTGTGATGG+TGG 0.611383 4:+23748353 MsaT019147.1:CDS
TACCACATGGCAATGGAGGA+GGG 0.611420 4:-23746789 None:intergenic
GCAACACGACATGGGTCACA+AGG 0.612121 4:-23746067 None:intergenic
ACTGTGGCTGTTCCGAACAC+AGG 0.612314 4:+23748008 MsaT019147.1:CDS
TTAACATTGGTTGGTAACAA+GGG 0.612869 4:-23746274 None:intergenic
ACTTATGAGCTTCCAACAGA+GGG 0.612897 4:+23745424 None:intergenic
TGTTTATCAGGGAGCTACTG+TGG 0.613577 4:+23747992 MsaT019147.1:CDS
ACTTCATGTGGGCAATCATG+TGG 0.615117 4:+23747090 MsaT019147.1:CDS
GACGGTGTACCACATGGCAA+TGG 0.615908 4:-23746796 None:intergenic
GGACTGTCTTAAATCAAGAA+GGG 0.615951 4:+23748255 MsaT019147.1:CDS
TGGAGATTGCCCTCCCTGTG+TGG 0.617392 4:+23746888 MsaT019147.1:CDS
CACCATTGACAGGTTATACG+TGG 0.617808 4:+23748099 MsaT019147.1:CDS
CAAATGACCGGGGAGGAGCA+GGG 0.618873 4:+23748054 MsaT019147.1:CDS
ACTAAAGAATTAAATGCAGG+AGG 0.619091 4:-23747786 None:intergenic
TTACAATGATGCAACCCGCA+AGG 0.619156 4:-23746391 None:intergenic
ATCCATTGCTGACGACGACG+AGG 0.619360 4:-23745458 None:intergenic
GGTGTACCACATGGCAATGG+AGG 0.619772 4:-23746793 None:intergenic
GAACATGGAAGTTGACATGA+AGG 0.620013 4:-23746820 None:intergenic
TCAAATCTAGAACACAAGCA+TGG 0.621162 4:+23748377 MsaT019147.1:CDS
GAAAAGAGACGACAAATCGA+GGG 0.622553 4:-23747818 None:intergenic
ACCTCAATTGTACAACCAAG+TGG 0.622833 4:+23745639 MsaT019147.1:CDS
CCACAAGGGCACAAACGAGG+AGG 0.624576 4:-23745926 None:intergenic
AATATAGTTTCAAGACAAGG+TGG 0.626822 4:-23746730 None:intergenic
GAGCTGCAACTGCAATGAGA+AGG 0.627931 4:+23747955 MsaT019147.1:CDS
GATCATGGAAGTGTTTATCA+GGG 0.628962 4:+23747981 MsaT019147.1:CDS
TGTGGAAAGAAGCTACGTTG+TGG 0.630668 4:+23746667 MsaT019147.1:CDS
CACTTATGAGCTTCCAACAG+AGG 0.630722 4:+23745423 None:intergenic
AGGCTGATTATCACAAGGTG+AGG 0.633317 4:-23747049 None:intergenic
ATCCCTCCTCCATTGCCATG+TGG 0.633425 4:+23746787 MsaT019147.1:CDS
TAATAGTAATCTTGCCACAA+GGG 0.634106 4:-23745940 None:intergenic
GAACATACCATGTAGCTCAA+GGG 0.635005 4:+23746954 MsaT019147.1:CDS
CTTGCTACAGGAGCCACACA+GGG 0.635180 4:-23746901 None:intergenic
CTACAGGAGCCACACAGGGA+GGG 0.635331 4:-23746897 None:intergenic
GTACCTGATGGAATTGGATG+AGG 0.636998 4:-23745735 None:intergenic
ATTCAGGACAAGCTCACCAA+AGG 0.637157 4:+23745533 MsaT019147.1:CDS
GGCGTGAGATAAAAGTCTGA+TGG 0.637472 4:-23745788 None:intergenic
ATAAGATGTAATAAGCTCTG+TGG 0.639598 4:+23746979 MsaT019147.1:CDS
AAACTTGCACCTCTCCTCGA+CGG 0.640734 4:-23747529 None:intergenic
CGTCTACTGCAACACGACAT+GGG 0.643358 4:-23746075 None:intergenic
GGAAGCCTCTTCAGAAGACG+GGG 0.645574 4:+23745834 MsaT019147.1:CDS
TGGGAGACTAAAACTGAACA+AGG 0.650928 4:-23746442 None:intergenic
AAGGTATTCGAACTTCATGT+GGG 0.652873 4:+23747079 MsaT019147.1:CDS
CCTGTAGCAAGAGAATGTGT+TGG 0.652958 4:+23746913 MsaT019147.1:CDS
AACCGGTCGGTAGTTGAGGG+TGG 0.656166 4:-23745488 None:intergenic
GCTACAGGAGCCACACAGGG+AGG 0.656807 4:-23746898 None:intergenic
ATATAGTTTCAAGACAAGGT+GGG 0.657550 4:-23746729 None:intergenic
GACAACTATGGGATGCTGGA+TGG 0.657750 4:-23746861 None:intergenic
TGGGATGCTGGATGGCCACA+AGG 0.658382 4:-23746853 None:intergenic
TTAGTTTGGGAAGATGAAGT+GGG 0.658868 4:-23745671 None:intergenic
TTGCCACAAGGGCACAAACG+AGG 0.662924 4:-23745929 None:intergenic
CAAGGTTCTCCACATGAATG+CGG 0.665398 4:-23745809 None:intergenic
GTCTCCCAGAAGTGTCGGTG+TGG 0.665685 4:+23746457 MsaT019147.1:CDS
GTAATAGTAATCTTGCCACA+AGG 0.666378 4:-23745941 None:intergenic
GTAGCAAATCATGCATTCGA+CGG 0.668746 4:-23745558 None:intergenic
TATCTGGACATGGAGATGAA+GGG 0.670313 4:-23747158 None:intergenic
CCTCAATTGTACAACCAAGT+GGG 0.670726 4:+23745640 MsaT019147.1:CDS
GAAGGCGACGGTGTACCACA+TGG 0.671013 4:-23746802 None:intergenic
GTAGCAAGAGAATGTGTTGG+TGG 0.671841 4:+23746916 MsaT019147.1:CDS
GTGAATATAGTTTCAAGACA+AGG 0.672220 4:-23746733 None:intergenic
GGAAGTTGACATGAAGGCGA+CGG 0.672285 4:-23746814 None:intergenic
AAGGAGCTGTGCATGTATGT+CGG 0.673465 4:-23747131 None:intergenic
TGAGATAAAAGTCTGATGGA+GGG 0.673701 4:-23745784 None:intergenic
CATTGCAAGACACAAACATG+AGG 0.674327 4:-23745866 None:intergenic
ACTAGTGCATGACCATATAG+GGG 0.676401 4:-23745600 None:intergenic
AAGGGTAAAGGAACTACACA+TGG 0.676858 4:+23747564 MsaT019147.1:CDS
GGGGCAAATGCTTTGCAAGG+AGG 0.683344 4:-23745905 None:intergenic
GATAATCAGCCTGTTGTGCA+AGG 0.684556 4:+23747060 MsaT019147.1:CDS
CACTACCACCAGCATCACAA+GGG 0.684650 4:-23747239 None:intergenic
CAAGGAATGGAACAGAGATG+CGG 0.687354 4:-23746646 None:intergenic
GTTGCACAGATTGACAGCCA+GGG 0.687497 4:-23745709 None:intergenic
AAGTTGAGGTAATAACCGGT+CGG 0.687832 4:-23745501 None:intergenic
TTGGTCATCAGAACAACGAG+TGG 0.689327 4:-23745963 None:intergenic
ATTGCAAGACACAAACATGA+GGG 0.693514 4:-23745865 None:intergenic
GAAGGCATCGTTGTAGTGAA+CGG 0.694226 4:+23746572 MsaT019147.1:CDS
GCAAAGTCAGAATCTTGTGA+TGG 0.696650 4:+23748350 MsaT019147.1:CDS
TAGCAAGAGAATGTGTTGGT+GGG 0.701673 4:+23746917 MsaT019147.1:CDS
TAGCATGGGACAGAAAACAT+GGG 0.706874 4:-23747595 None:intergenic
ATTCTTGGGATGATCAAGAG+GGG 0.708270 4:+23748159 MsaT019147.1:CDS
ACAAACTGAAAGTAGCGTTG+TGG 0.714145 4:+23748328 MsaT019147.1:CDS
AGGATAAGAGAGATGCTGTG+AGG 0.719014 4:+23747619 MsaT019147.1:CDS
ACAACAGGCTGATTATCACA+AGG 0.719205 4:-23747054 None:intergenic
TCAGTTACAAATGACCGGGG+AGG 0.724073 4:+23748047 MsaT019147.1:CDS
TGTGGAAACAAACTTGCATG+TGG 0.727941 4:+23746202 MsaT019147.1:CDS
AACGTCCCTTGTGATGCTGG+TGG 0.746682 4:+23747234 MsaT019147.1:CDS
TGTGGAAAGACCAAACAGTG+CGG 0.748915 4:+23746997 MsaT019147.1:CDS
TCTTCAGTTACAAATGACCG+GGG 0.749426 4:+23748044 MsaT019147.1:CDS
GGAGCAGGGATAGTGAAAGG+CGG 0.755928 4:+23748068 MsaT019147.1:CDS
TGTGATTCCAATTGTCACAG+TGG 0.757912 4:+23746700 MsaT019147.1:CDS
GAAGCCTCTTCAGAAGACGG+GGG 0.769844 4:+23745835 MsaT019147.1:CDS
GGAGTGTTGTAAGAAAACAA+TGG 0.800295 4:+23746498 MsaT019147.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 23745428 23748463 23745428 ID=MsaG019147
Chr4 mRNA 23745428 23748463 23745428 ID=MsaT019147.1;Parent=MsaG019147
Chr4 exon 23745428 23748463 23745428 ID=MsaT019147.1.exon1;Parent=MsaT019147.1
Chr4 CDS 23745428 23748463 23745428 ID=cds.MsaT019147.1;Parent=MsaT019147.1
Gene Sequence

>MsaT019147.1

ATGAGCTTCCAACAGAGGGAAATTAGGAATCCTCGTCGTCGTCAGCAATGGATTCCCAGACCACCCTCAACTACCGACCGGTTATTACCTCAACTTCTGCAAGAGATTCAGGACAAGCTCACCAAAGGCACCGTCGAATGCATGATTTGCTACGACACTGTCCGCAGATCTTCCCCTATATGGTCATGCACTAGTTGCTACTCCATCTTTCACCTCAATTGTACAACCAAGTGGGCTCGTGCACCCACTTCATCTTCCCAAACTAACATTGATTGGCGCTGCCCTGGCTGTCAATCTGTGCAACTCACCTCATCCAATTCCATCAGGTACTTCTGCTTTTGTGGGAAGACGCCCGACCCTCCATCAGACTTTTATCTCACGCCGCATTCATGTGGAGAACCTTGTGGGAAGCCTCTTCAGAAGACGGGGGATAATTGCCCTCATGTTTGTGTCTTGCAATGTCATCCTGGTCCATGTCCTCCTTGCAAAGCATTTGCCCCTCCTCGTTTGTGCCCTTGTGGCAAGATTACTATTACCACTCGTTGTTCTGATGACCAATCTCTTATTACATGTGGACAACAATGTCAAAAGCTTCTTCAATGTGAGCGTCATCGCTGTCACCAAATTTGTCATCTCGGTCCTTGTGACCCATGTCGTGTTGCAGTAGACGCCTCTTGCTTTTGCAGGAAAAATTTGGAGGTTATAATTTGTGGGGACATGGCTGTAAAGGGTGAATACAAAGCAGAAGCTGGAGTTTTTTCCTGTGGTTCTACCTGTGGAAACAAACTTGCATGTGGTAATCACGTATGTATTGAGACCTGTCATCCGGGAAGTTGTGTTGAGTGTCCCTTGTTACCAACCAATGTTAAGACATGCTTCTGCGGGAAAACTAGGTTGGGCGAGGAACGGCTGAGTTGTTTAGACCCAATTCCTACATGTTCACAAGTATGTGGCAAGTCTCTTCCTTGCGGGTTGCATCATTGTAAAATGACATGCCATGATGGGAATTGTTCTCCTTGTTCAGTTTTAGTCTCCCAGAAGTGTCGGTGTGGCTCAACTTCCAAAAGTGTGGAGTGTTGTAAGAAAACAATGGCGAATGAGACATTTACTTGTGAAAAGCCTTGTGGGAAGAAAAAGAATTGTGGAAGGCATCGTTGTAGTGAACGGTGTTGTCCACTCTCTAATCCAAATAATATTCTAAATGCAACGGATTGGAATCCGCATCTCTGTTCCATTCCTTGTGGAAAGAAGCTACGTTGTGGGAAGCATGCGTGTGATTCCAATTGTCACAGTGGTCATTGCCCACCTTGTCTTGAAACTATATTCACTGATTTGACATGTGCTTGTGGCAAGACTTCAATCCCTCCTCCATTGCCATGTGGTACACCGTCGCCTTCATGTCAACTTCCATGTTCAGTTCCTCAACCTTGTGGCCATCCAGCATCCCATAGTTGTCATCATGGAGATTGCCCTCCCTGTGTGGCTCCTGTAGCAAGAGAATGTGTTGGTGGGCATGTAGTTCTTAGGAACATACCATGTAGCTCAAGGGATATAAGATGTAATAAGCTCTGTGGAAAGACCAAACAGTGCGGTTTACATGCTTGTAGCAGAATATGTCACTCCTCACCTTGTGATAATCAGCCTGTTGTGCAAGGTATTCGAACTTCATGTGGGCAATCATGTGGCGCTCCGAGGAGAGACTGCCGACATACATGCACAGCTCCTTGTCACCCTTCATCTCCATGTCCAGATAGAAGATGTGGATTCGCTGTCACAATCACTTGTACCTGTGGTCGTTTAACAACAAACGTCCCTTGTGATGCTGGTGGTAGTGGTGTTGATTCTATGCATGAAGCTTCTATTATTCAAAAGTTGCTTGTGCCACTTCAACCATTGGATGCAAATGGCAAAAAAGTTCCCCTTGGACAGCGAAAACTGATGTGTAATGATGAGTGCGCTAAGTTGGAGCGTAAAAGGGTTCTTGCAAATGCTTTTGAGATTAGCACTCCAAATCTAGATTCTTTCCAATTTGGTGAGAACTCTGTTGCTCATTCTGAATTACTTGCTGATTTATTGAGGCGTGATAGTAAATGGGTTTTGTCCGTCGAGGAGAGGTGCAAGTTTTTAGTACTTGGCAAGGGTAAAGGAACTACACATGGTCTAAAATCCCATGTTTTCTGTCCCATGCTAAAGGATAAGAGAGATGCTGTGAGGATGATTGCTGATAGATGGAAGCTTGCAATAAATGAAGCCGGTCGGGAGCCAAAGAAATTTATAGTTGTTCATGTTGTCCAAAAATCAAGAGCTCCATCACGCGTGCTAGGGATTAAGGGTACTACAACCGTACCTTCTCTCCTTCCTCCTGCATTTAATTCTTTAGTAGACATGGGCCCTCGATTTGTCGTCTCTTTTCCAGACTTACCAAGAGATGCAAATATCAGTGCATTGGTGTTGAGATTTGGTGGTGAGTGCGAACTTGTTTGGTTAAATGATAAAAATGCATTGGCTGTTTTTAACGATCCTGCCCGAGCTGCAACTGCAATGAGAAGGTTGGATCATGGAAGTGTTTATCAGGGAGCTACTGTGGCTGTTCCGAACACAGGAACATCTGTAGCATCTTCAGTTACAAATGACCGGGGAGGAGCAGGGATAGTGAAAGGCGGTGCGCTGACACCATTGACAGGTTATACGTGGAAAAAGGCTGTTATTGTAGAACCAGCTTGTAGAAAAGATTCTTGGGATGATCAAGAGGGGACTACTGATTGTGCAAATATTCAATCATCTATATGGAAGAAAGAAACAACAATTACTACTTCATCAAATCCTTGGACTGTCTTAAATCAAGAAGGGTCTTCAAGTTCATCTGCTAAAGCTTCTGTTAAAGCTGACACTTCTATCAAACAAACTGAAAGTAGCGTTGTGGCAAAGTCAGAATCTTGTGATGGTGGTTCAAATCTAGAACACAAGCATGGAAGAGCATTTCACACTTTGGAAGTTTATGATGTAGTAGATGATTGGGAGACAACTTGTGAATGA
Protein sequence

>MsaT019147.1

MSFQQREIRNPRRRQQWIPRPPSTTDRLLPQLLQEIQDKLTKGTVECMICYDTVRRSSPIWSCTSCYSIFHLNCTTKWARAPTSSSQTNIDWRCPGCQSVQLTSSNSIRYFCFCGKTPDPPSDFYLTPHSCGEPCGKPLQKTGDNCPHVCVLQCHPGPCPPCKAFAPPRLCPCGKITITTRCSDDQSLITCGQQCQKLLQCERHRCHQICHLGPCDPCRVAVDASCFCRKNLEVIICGDMAVKGEYKAEAGVFSCGSTCGNKLACGNHVCIETCHPGSCVECPLLPTNVKTCFCGKTRLGEERLSCLDPIPTCSQVCGKSLPCGLHHCKMTCHDGNCSPCSVLVSQKCRCGSTSKSVECCKKTMANETFTCEKPCGKKKNCGRHRCSERCCPLSNPNNILNATDWNPHLCSIPCGKKLRCGKHACDSNCHSGHCPPCLETIFTDLTCACGKTSIPPPLPCGTPSPSCQLPCSVPQPCGHPASHSCHHGDCPPCVAPVARECVGGHVVLRNIPCSSRDIRCNKLCGKTKQCGLHACSRICHSSPCDNQPVVQGIRTSCGQSCGAPRRDCRHTCTAPCHPSSPCPDRRCGFAVTITCTCGRLTTNVPCDAGGSGVDSMHEASIIQKLLVPLQPLDANGKKVPLGQRKLMCNDECAKLERKRVLANAFEISTPNLDSFQFGENSVAHSELLADLLRRDSKWVLSVEERCKFLVLGKGKGTTHGLKSHVFCPMLKDKRDAVRMIADRWKLAINEAGREPKKFIVVHVVQKSRAPSRVLGIKGTTTVPSLLPPAFNSLVDMGPRFVVSFPDLPRDANISALVLRFGGECELVWLNDKNALAVFNDPARAATAMRRLDHGSVYQGATVAVPNTGTSVASSVTNDRGGAGIVKGGALTPLTGYTWKKAVIVEPACRKDSWDDQEGTTDCANIQSSIWKKETTITTSSNPWTVLNQEGSSSSSAKASVKADTSIKQTESSVVAKSESCDGGSNLEHKHGRAFHTLEVYDVVDDWETTCE*