AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019169

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019169 MtrunA17_Chr4g0012761 98.113 159 3 0 1 159 1 159 1.65e-114 321
MsaG019169 MtrunA17_Chr7g0218151 91.824 159 13 0 1 159 1 159 7.20e-100 284
MsaG019169 MtrunA17_Chr1g0188661 87.421 159 20 0 1 159 1 159 2.34e-93 267
MsaG019169 MtrunA17_Chr5g0420351 71.154 156 45 0 1 156 1 156 7.67e-78 228
MsaG019169 MtrunA17_Chr1g0188681 70.701 157 46 0 1 157 1 157 9.35e-78 228
MsaG019169 MtrunA17_Chr1g0193311 76.282 156 37 0 1 156 1 156 1.32e-76 225
MsaG019169 MtrunA17_Chr1g0188651 75.472 159 37 1 1 159 1 157 6.54e-74 218
MsaG019169 MtrunA17_Chr1g0197261 54.839 155 70 0 1 155 1 155 1.04e-57 177
MsaG019169 MtrunA17_Chr1g0197321 53.846 156 71 1 1 155 1 156 9.35e-57 174
MsaG019169 MtrunA17_Chr1g0197461 54.248 153 70 0 3 155 2 154 6.66e-55 170
MsaG019169 MtrunA17_Chr4g0014321 40.000 155 91 2 1 153 1 155 2.26e-40 135
MsaG019169 MtrunA17_Chr3g0107381 45.098 153 83 1 1 152 1 153 5.10e-40 134
MsaG019169 MtrunA17_Chr3g0110131 48.408 157 80 1 1 156 1 157 2.72e-38 130
MsaG019169 MtrunA17_Chr4g0013041 38.065 155 92 3 1 153 1 153 1.93e-35 122
MsaG019169 MtrunA17_Chr5g0432691 37.821 156 96 1 1 155 1 156 4.96e-35 119
MsaG019169 MtrunA17_Chr3g0107331 42.038 157 90 1 3 158 2 158 5.67e-34 117
MsaG019169 MtrunA17_Chr8g0351751 38.994 159 93 2 1 155 1 159 5.21e-33 117
MsaG019169 MtrunA17_Chr3g0107351 42.208 154 88 1 3 155 2 155 1.04e-31 111
MsaG019169 MtrunA17_Chr3g0107361 40.909 154 90 1 3 155 2 155 1.96e-31 110
MsaG019169 MtrunA17_Chr3g0107341 40.909 154 90 1 3 155 2 155 4.46e-31 109
MsaG019169 MtrunA17_Chr4g0014031 42.000 150 85 2 1 148 1 150 1.12e-30 110
MsaG019169 MtrunA17_Chr3g0107461 34.783 161 103 2 1 159 1 161 1.17e-29 106
MsaG019169 MtrunA17_Chr4g0014121 41.875 160 91 2 1 158 29 188 1.86e-29 106
MsaG019169 MtrunA17_Chr2g0283941 38.750 160 94 3 1 157 1 159 1.60e-28 105
MsaG019169 MtrunA17_Chr4g0014131 39.752 161 95 2 1 159 1 161 2.84e-27 102
MsaG019169 MtrunA17_Chr2g0296861 35.484 155 99 1 1 154 1 155 2.27e-24 92.4
MsaG019169 MtrunA17_Chr2g0296831 40.678 118 69 1 1 117 1 118 2.59e-24 91.3
MsaG019169 MtrunA17_Chr3g0105791 35.115 131 83 2 29 157 1 131 1.10e-21 85.1
MsaG019169 MtrunA17_Chr4g0031421 34.351 131 84 2 29 157 1 131 4.46e-21 83.6
MsaG019169 MtrunA17_Chr4g0008701 36.220 127 80 1 3 128 6 132 5.58e-19 82.8
MsaG019169 MtrunA17_Chr7g0267601 37.963 108 66 1 3 109 6 113 3.74e-18 80.5
MsaG019169 MtrunA17_Chr3g0106501 30.534 131 89 2 29 157 1 131 1.75e-15 69.3
MsaG019169 MtrunA17_Chr3g0106421 30.233 129 88 2 29 155 1 129 2.25e-14 67.8
MsaG019169 MtrunA17_Chr1g0161931 42.553 94 53 1 64 156 28 121 2.03e-13 63.2
MsaG019169 MtrunA17_Chr1g0162181 40.816 98 57 1 62 158 20 117 2.70e-13 62.8
MsaG019169 MtrunA17_Chr1g0162261 38.776 98 59 1 62 158 20 117 3.59e-13 62.4
MsaG019169 MtrunA17_Chr3g0128721 30.345 145 95 3 3 143 6 148 7.68e-13 65.1
MsaG019169 MtrunA17_Chr6g0449861 31.373 102 64 3 1 96 9 110 3.45e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019169 AT5G48670.1 46.405 153 81 1 1 152 1 153 1.38e-33 120
MsaG019169 AT3G05860.2 38.926 149 90 1 1 148 1 149 5.52e-31 111
MsaG019169 AT3G05860.3 38.926 149 90 1 1 148 1 149 3.16e-30 110
MsaG019169 AT3G05860.1 38.926 149 90 1 1 148 1 149 4.38e-30 110
MsaG019169 AT5G26630.1 38.125 160 98 1 1 159 1 160 3.08e-29 107
MsaG019169 AT1G65300.1 36.620 142 89 1 3 143 2 143 1.65e-27 103
MsaG019169 AT1G65330.1 36.735 147 92 1 3 148 2 148 5.50e-27 102
MsaG019169 AT5G26650.1 33.987 153 100 1 4 155 2 154 1.88e-24 97.4
MsaG019169 AT5G27960.1 33.333 153 101 1 4 155 2 154 2.93e-23 93.6
MsaG019169 AT1G31630.1 29.677 155 107 2 3 156 2 155 9.37e-22 89.7
MsaG019169 AT1G22590.2 41.304 92 47 1 1 85 1 92 1.77e-21 85.5
MsaG019169 AT2G28700.1 33.557 149 98 1 1 148 1 149 2.29e-20 85.9
MsaG019169 AT5G27810.1 42.708 96 54 1 29 123 1 96 1.02e-19 79.7
MsaG019169 AT1G31640.1 31.333 150 101 2 3 151 2 150 1.30e-18 82.0
MsaG019169 AT5G26580.1 31.613 155 93 1 1 155 1 142 4.38e-18 79.7
MsaG019169 AT2G40210.1 35.000 140 85 3 1 137 1 137 1.19e-14 70.5
MsaG019169 AT5G06500.1 30.380 158 108 2 1 157 1 157 2.31e-14 68.6
MsaG019169 AT5G58890.1 35.417 96 55 2 1 89 1 96 3.95e-12 62.8

Find 26 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCAAAAGGAAACTTTCT+TGG 0.256030 4:+24329406 None:intergenic
AGCTGAACATTCTTATGATT+TGG 0.274450 4:-24329326 MsaT019169.1:CDS
CGCCTTTCTTGTTGAATCAT+TGG 0.287726 4:+24329623 None:intergenic
ACCTTTCTTCCTCCGCTTGT+AGG 0.316491 4:+24329599 None:intergenic
TGTTTGGCTTCCTTTAGATT+TGG 0.353502 4:+24329483 None:intergenic
ATACCTCTCAATCATCTGTT+TGG 0.362514 4:+24329467 None:intergenic
CAGAGCTCATATATGAAAGA+TGG 0.456034 4:-24329444 MsaT019169.1:CDS
CTCCAATGATTCAACAAGAA+AGG 0.460238 4:-24329625 MsaT019169.1:CDS
TCAGAAACAGTCACATCATC+TGG 0.464679 4:+24329282 None:intergenic
TGTTTCTGATTTGAAAGACT+TGG 0.473410 4:-24329268 MsaT019169.1:CDS
TGCAAAAGATTACTAAAGCT+AGG 0.481563 4:-24329389 MsaT019169.1:CDS
GCAAAAGATTACTAAAGCTA+GGG 0.487712 4:-24329388 MsaT019169.1:CDS
ACCTACAAGCGGAGGAAGAA+AGG 0.494539 4:-24329600 MsaT019169.1:CDS
CAAAGTCATGGCTAGGAAGA+AGG 0.502352 4:-24329664 None:intergenic
AGAGGTGTGGCCAAATCTAA+AGG 0.522555 4:-24329493 MsaT019169.1:CDS
AGAGAACTCACTATTCTTTG+TGG 0.526508 4:-24329561 MsaT019169.1:CDS
GAAGGAAATTGATGACAAGA+TGG 0.549956 4:-24329223 MsaT019169.1:CDS
TCGGAAATTATAGCACATGC+TGG 0.559487 4:+24329534 None:intergenic
AGAGCTCATATATGAAAGAT+GGG 0.560272 4:-24329443 MsaT019169.1:CDS
TACATTGCAAAGTCATGGCT+AGG 0.590557 4:-24329671 None:intergenic
GAAGAAAGGTATCATCAAGA+AGG 0.595296 4:-24329586 MsaT019169.1:CDS
GAAAGGCGACCTACAAGCGG+AGG 0.629626 4:-24329608 MsaT019169.1:CDS
AAGCCAAACAGATGATTGAG+AGG 0.630265 4:-24329470 MsaT019169.1:CDS
TTGATTCTAAAACAGAGGTG+TGG 0.633348 4:-24329506 MsaT019169.1:CDS
CAAGAAAGGCGACCTACAAG+CGG 0.654193 4:-24329611 MsaT019169.1:CDS
TTGGTACCTACAAAACAACA+AGG 0.712897 4:-24329307 MsaT019169.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 24329200 24329679 24329200 ID=MsaG019169
Chr4 mRNA 24329200 24329679 24329200 ID=MsaT019169.1;Parent=MsaG019169
Chr4 exon 24329200 24329679 24329200 ID=MsaT019169.1.exon1;Parent=MsaT019169.1
Chr4 CDS 24329200 24329679 24329200 ID=cds.MsaT019169.1;Parent=MsaT019169.1
Gene Sequence

>MsaT019169.1

ATGGCTAGGAAGAAGGTGAAACTCGCTTTTATCTCCAATGATTCAACAAGAAAGGCGACCTACAAGCGGAGGAAGAAAGGTATCATCAAGAAGGTGAGAGAACTCACTATTCTTTGTGGTATTCCAGCATGTGCTATAATTTCCGATCCTTTTGATTCTAAAACAGAGGTGTGGCCAAATCTAAAGGAAGCCAAACAGATGATTGAGAGGTATCAGAGCTCATATATGAAAGATGGGAGAAAAAATGTGAACCAAGAAAGTTTCCTTTTGCAAAAGATTACTAAAGCTAGGGAGCAACTGAGAATACAAAGACACGACAATCATGAGAATGAGCTGAACATTCTTATGATTTGGTACCTACAAAACAACAAGGTGCCAGATGATGTGACTGTTTCTGATTTGAAAGACTTGGATAAGTTGATTGAAAAAAATATGAAGGAAATTGATGACAAGATGGCTTCACTTAGTTTATCAAACTGA
Protein sequence

>MsaT019169.1

MARKKVKLAFISNDSTRKATYKRRKKGIIKKVRELTILCGIPACAIISDPFDSKTEVWPNLKEAKQMIERYQSSYMKDGRKNVNQESFLLQKITKAREQLRIQRHDNHENELNILMIWYLQNNKVPDDVTVSDLKDLDKLIEKNMKEIDDKMASLSLSN*