AlfalfaGEDB | MsaG019180

M. sativa ssp. caerulea / MsaG019180

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG019180	XP_003605289.1	96.970	165	5	0	678	842	736	900	3.51e-90	315
MsaG019180	XP_003605289.1	97.784	677	14	1	1	677	1	676	0.0	1387

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG019180	sp\|Q6Z3U3\|Y7797_ORYSJ	44.039	931	394	18	4	826	15	926	0.0	675

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG019180	tr\|G7JIB0\|G7JIB0_MEDTR	96.970	165	5	0	678	842	736	900	1.68e-90	315
MsaG019180	tr\|G7JIB0\|G7JIB0_MEDTR	97.784	677	14	1	1	677	1	676	0.0	1387

TFs/TRs:

Gene ID	Type	Classification
MsaG019180	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000157	MsaG019180	0.806561	1.313659e-49	2.065940e-47
MsaG001084	MsaG019180	0.821502	6.860739e-53	1.570098e-50
MsaG001179	MsaG019180	0.811324	1.270771e-50	2.241482e-48
MsaG002013	MsaG019180	0.814918	2.085509e-51	4.023181e-49
MsaG003210	MsaG019180	0.829322	9.852480e-55	2.796445e-52
MsaG003203	MsaG019180	0.807813	7.155398e-50	1.159240e-47
MsaG003868	MsaG019180	0.811768	1.018706e-50	1.816478e-48
MsaG003954	MsaG019180	0.800497	2.344337e-48	3.205452e-46
MsaG005508	MsaG019180	0.828519	1.538308e-54	4.268627e-52
MsaG005864	MsaG019180	0.833634	8.647916e-56	2.781307e-53
MsaG006175	MsaG019180	0.818534	3.253423e-52	6.884977e-50
MsaG006867	MsaG019180	0.830124	6.298823e-55	1.829432e-52
MsaG008552	MsaG019180	0.816440	9.591834e-52	1.923141e-49
MsaG009390	MsaG019180	0.810165	2.257164e-50	3.870077e-48
MsaG010053	MsaG019180	0.819113	2.406603e-52	5.170869e-50
MsaG010299	MsaG019180	0.823940	1.868893e-53	4.567260e-51
MsaG010835	MsaG019180	0.847061	2.767561e-59	1.358026e-56
MsaG010894	MsaG019180	0.801385	1.547166e-48	2.158331e-46
MsaG010899	MsaG019180	0.828608	1.464333e-54	4.073431e-52
MsaG011806	MsaG019180	0.817319	6.102566e-52	1.251615e-49
MsaG012304	MsaG019180	0.813399	4.498284e-51	8.352823e-49
MsaG012365	MsaG019180	0.800997	1.855849e-48	2.566286e-46
MsaG012894	MsaG019180	0.800995	1.857369e-48	2.568273e-46
MsaG017058	MsaG019180	0.833678	8.433751e-56	2.716026e-53
MsaG017718	MsaG019180	0.818668	3.034289e-52	6.443660e-50
MsaG017820	MsaG019180	0.833559	9.028071e-56	2.897141e-53
MsaG018248	MsaG019180	0.815329	1.692472e-51	3.299038e-49
MsaG019180	MsaG021863	0.823943	1.866412e-53	4.561525e-51
MsaG019180	MsaG023271	0.808445	5.255200e-50	8.644245e-48
MsaG019180	MsaG025484	0.819564	1.901176e-52	4.133326e-50
MsaG019180	MsaG026714	0.814180	3.032292e-51	5.742025e-49
MsaG019180	MsaG027235	0.808919	4.166693e-50	6.932566e-48
MsaG019180	MsaG028034	0.815267	1.746264e-51	3.398662e-49
MsaG019180	MsaG028500	0.815656	1.432510e-51	2.815594e-49
MsaG019180	MsaG028501	0.808078	6.289090e-50	1.025390e-47
MsaG019180	MsaG028716	0.804543	3.467180e-49	5.201027e-47
MsaG019180	MsaG029320	0.821692	6.204821e-53	1.427197e-50
MsaG019180	MsaG029446	0.826118	5.751388e-54	1.492260e-51
MsaG019180	MsaG032062	0.811030	1.470635e-50	2.575361e-48
MsaG019180	MsaG034172	0.801287	1.620013e-48	2.254908e-46
MsaG019180	MsaG034827	0.835137	3.643705e-56	1.225488e-53
MsaG019180	MsaG034828	0.808273	5.717504e-50	9.365889e-48
MsaG019180	MsaG034829	0.843474	2.558110e-58	1.115288e-55
MsaG019180	MsaG034842	0.814685	2.348582e-51	4.504004e-49
MsaG019180	MsaG036332	0.825548	7.840554e-54	2.002308e-51
MsaG019180	MsaG037719	0.822425	4.203507e-53	9.859989e-51
MsaG019180	MsaG041899	0.850585	2.942432e-60	1.627342e-57
MsaG019180	MsaG041900	0.851983	1.190846e-60	6.914353e-58
MsaG019180	MsaG041901	0.833710	8.279464e-56	2.668817e-53
MsaG019180	MsaG041902	0.847905	1.626055e-59	8.208894e-57
MsaG019180	MsaG044336	0.818868	2.734589e-52	5.837627e-50
MsaG019180	MsaG044439	0.818683	3.010536e-52	6.395694e-50
MsaG019180	MsaG046383	0.821810	5.827146e-53	1.344488e-50
MsaG019180	MsaG047055	0.809942	2.520391e-50	4.297996e-48
MsaG019180	MsaG002456	0.819890	1.603082e-52	3.515473e-50
MsaG019180	MsaG008937	0.805264	2.454307e-49	3.743699e-47
MsaG019180	MsaG008796	0.818514	3.287224e-52	6.952902e-50
MsaG019180	MsaG009037	0.827050	3.455620e-54	9.201216e-52
MsaG019180	MsaG031316	0.800555	2.281997e-48	3.124359e-46
MsaG019180	MsaG033325	0.807765	7.324440e-50	1.185246e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG019180	MtrunA17_Chr4g0012861	97.784	677	14	1	1	677	1	676	0.0	1387
MsaG019180	MtrunA17_Chr4g0012861	96.970	165	5	0	678	842	736	900	3.23e-94	315
MsaG019180	MtrunA17_Chr3g0107701	50.073	689	307	8	19	676	16	698	0.0	643
MsaG019180	MtrunA17_Chr5g0432721	42.525	689	338	9	1	676	1	644	3.39e-163	494
MsaG019180	MtrunA17_Chr2g0281191	54.187	406	147	5	270	675	308	674	3.21e-133	419
MsaG019180	MtrunA17_Chr2g0281191	43.411	129	56	2	4	132	29	140	9.41e-27	117
MsaG019180	MtrunA17_Chr7g0244871	68.478	92	29	0	308	399	6	97	1.33e-40	144
MsaG019180	MtrunA17_Chr7g0251891	37.838	111	68	1	314	423	16	126	3.86e-19	86.7
MsaG019180	MtrunA17_Chr7g0237841	35.714	98	62	1	314	410	623	720	8.10e-15	79.0
MsaG019180	MtrunA17_Chr1g0178071	36.090	133	65	6	295	414	54	179	1.35e-14	75.9
MsaG019180	MtrunA17_Chr8g0345771	37.273	110	61	4	314	417	171	278	2.84e-14	76.6
MsaG019180	MtrunA17_Chr7g0276471	40.659	91	49	3	314	401	117	205	7.12e-14	75.1
MsaG019180	MtrunA17_Chr7g0244881	41.441	111	62	2	199	306	3	113	2.83e-13	67.4
MsaG019180	MtrunA17_Chr7g0266821	39.252	107	58	5	319	421	65	168	7.81e-13	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG019180	AT4G32010.2	52.360	678	242	11	1	672	1	603	0.0	680
MsaG019180	AT4G32010.1	52.053	682	242	12	1	672	1	607	0.0	675
MsaG019180	AT2G30470.1	50.293	682	276	17	1	675	6	631	0.0	603
MsaG019180	AT4G21550.3	34.576	590	289	13	3	570	14	528	8.33e-95	308
MsaG019180	AT4G21550.1	34.915	590	288	11	3	571	14	528	9.56e-90	299
MsaG019180	AT4G21550.2	33.798	574	284	12	3	555	14	512	3.32e-89	292
MsaG019180	AT3G26790.1	39.450	109	65	1	314	421	91	199	9.67e-20	91.7
MsaG019180	AT5G06250.5	32.948	173	95	5	314	467	45	215	1.19e-16	81.3
MsaG019180	AT5G06250.4	32.948	173	95	5	314	467	45	215	1.28e-16	81.6
MsaG019180	AT5G06250.2	32.948	173	95	5	314	467	45	215	1.45e-16	81.6
MsaG019180	AT3G24650.1	37.931	116	70	2	314	427	571	686	1.84e-15	81.3
MsaG019180	AT1G13260.1	40.000	110	59	5	314	418	187	294	1.92e-15	79.3
MsaG019180	AT1G28300.1	40.741	108	60	3	294	398	149	255	3.43e-15	78.6
MsaG019180	AT2G36080.3	39.815	108	56	4	314	414	37	142	4.42e-15	76.3
MsaG019180	AT2G36080.1	39.815	108	56	4	314	414	37	142	4.55e-15	76.3
MsaG019180	AT2G36080.4	39.815	108	56	4	314	414	37	142	4.85e-15	74.3
MsaG019180	AT2G36080.2	39.815	108	56	4	314	414	37	142	4.85e-15	74.3
MsaG019180	AT5G06250.3	36.923	130	56	5	314	419	45	172	6.54e-15	76.3
MsaG019180	AT5G06250.1	33.103	145	81	4	314	444	45	187	6.70e-15	76.3
MsaG019180	AT4G01500.1	38.261	115	59	4	297	401	9	121	3.10e-14	75.5
MsaG019180	AT1G68840.2	43.011	93	46	4	314	401	187	277	4.04e-14	75.1
MsaG019180	AT1G68840.1	43.011	93	46	4	314	401	187	277	4.04e-14	75.1
MsaG019180	AT2G46870.1	39.560	91	50	3	314	401	34	122	8.46e-14	73.6
MsaG019180	AT3G11580.4	36.207	116	57	4	314	414	28	141	7.47e-13	69.3
MsaG019180	AT3G11580.2	36.207	116	57	4	314	414	28	141	7.47e-13	69.3
MsaG019180	AT3G11580.5	36.207	116	57	4	314	414	28	141	7.47e-13	69.3
MsaG019180	AT1G01030.2	40.659	91	49	3	314	401	55	143	8.37e-13	70.9
MsaG019180	AT1G01030.1	40.659	91	49	3	314	401	55	143	9.77e-13	71.2
MsaG019180	AT3G11580.3	36.207	116	57	4	314	414	28	141	4.71e-12	68.9
MsaG019180	AT3G11580.1	36.207	116	57	4	314	414	28	141	4.99e-12	67.8

Find 221 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATGTTTCAATTTAGATTT+TGG	0.133418	4:+24510937	MsaT019180.1:CDS
TACATCCTGCTATTATTATT+TGG	0.167699	4:-24510960	None:intergenic
CAACTCTTGATCTGTAATTC+TGG	0.172835	4:-24510475	None:intergenic
TTACCCTCTGCTGGAGAATT+TGG	0.185934	4:+24510099	MsaT019180.1:CDS
GCATACCCCTCACATTTAAT+TGG	0.234093	4:-24509978	None:intergenic
AAGAAAAGAACTCGAAATAT+TGG	0.272591	4:+24511985	MsaT019180.1:CDS
GGACGTGTATGGAGAACTTT+TGG	0.278484	4:+24512876	MsaT019180.1:CDS
TCAAGTGAAACTTCCTATTC+TGG	0.278901	4:+24511766	MsaT019180.1:CDS
GAGGCCTTGCTATACGTATC+TGG	0.288912	4:-24510407	None:intergenic
ATTACTTCAGCACATGCTTT+TGG	0.292122	4:-24510735	None:intergenic
CCAGCAGAGGGTAAGACTTC+TGG	0.293216	4:-24510090	None:intergenic
CCTATATTAAGTGGTTATTC+TGG	0.299020	4:+24511805	MsaT019180.1:CDS
CAAACCTCACAATGTTGATT+TGG	0.320944	4:+24510044	MsaT019180.1:CDS
ATCAGTTGCTTCCGCGATAT+TGG	0.327434	4:+24510451	MsaT019180.1:CDS
ATTCAGGAGCCTCCTGTGTT+TGG	0.329853	4:+24512593	MsaT019180.1:intron
CAACTGCAAGCTGGCGATAC+TGG	0.336996	4:+24511020	MsaT019180.1:CDS
TCTTGCCCACTATTCTCTTC+AGG	0.340208	4:-24514792	None:intergenic
ACAACACTTGGCTTAACTTC+TGG	0.343319	4:-24512099	None:intergenic
CCCTGAGGGGAAACTTATAA+TGG	0.343393	4:+24511602	MsaT019180.1:CDS
AGAGTTGGCCTAGGAGGTTT+AGG	0.345670	4:-24510348	None:intergenic
TTCCCTTGGATTCAATTCAT+TGG	0.348370	4:-24513009	None:intergenic
CACTGGAGCTGAAACTTACT+TGG	0.350730	4:+24512046	MsaT019180.1:CDS
AATGAAAAGCCTAATGAATC+TGG	0.351647	4:+24509904	MsaT019180.1:CDS
ACTTTCTAGAGAGCGCATTT+AGG	0.352085	4:-24511864	None:intergenic
TCAAGAGTTGCAGCAAATAT+CGG	0.354064	4:+24510488	MsaT019180.1:CDS
CGACATTTAACTCCAGTTGA+TGG	0.354939	4:+24514438	MsaT019180.1:CDS
TTTGCTGTTCCAGATTCATT+AGG	0.360782	4:-24509913	None:intergenic
TTACTTGAATCTAATTCATT+TGG	0.362360	4:-24514483	None:intergenic
TTGTCTTCCCTCACTCTCAT+TGG	0.362735	4:-24514721	None:intergenic
ATGTGAGGGGTATGCAAGTT+GGG	0.375012	4:+24509986	MsaT019180.1:CDS
CAGCATCAGCCACTTGGTCT+TGG	0.376062	4:-24514506	None:intergenic
TCCCTTGGATTCAATTCATT+GGG	0.378007	4:-24513008	None:intergenic
AGGACATTATGAAAGGGATT+TGG	0.378577	4:-24510255	None:intergenic
GTCTACTTCTGATTCATCCT+TGG	0.381182	4:-24514406	None:intergenic
AGGCCCTCAGGTTGAGAGAT+AGG	0.381204	4:-24510879	None:intergenic
TTAGCGAATGCTGCGGTACT+TGG	0.388603	4:+24514137	MsaT019180.1:CDS
ATTACCTGTGCAAGTACTTC+AGG	0.390989	4:+24509382	MsaT019180.1:CDS
ATATTTGTGGTACGATCTAC+TGG	0.393389	4:+24512626	MsaT019180.1:CDS
TGGCCTTCTTCAGTCTCAAA+AGG	0.396098	4:+24511825	MsaT019180.1:CDS
GCTCTGGATCGTGAATCTTC+TGG	0.398177	4:+24514104	MsaT019180.1:CDS
ACTTACTTGGGAAGAGGCTC+AGG	0.399619	4:+24512059	MsaT019180.1:CDS
GTCATCATGCTCAGAGTGTT+TGG	0.401168	4:-24514585	None:intergenic
GGAAACTTATAATGGGGTTC+AGG	0.403096	4:+24511610	MsaT019180.1:CDS
CAATTTCATCGTGATTCAAA+TGG	0.411668	4:+24510138	MsaT019180.1:CDS
TTCCTTCCTCGTCCTTCGGC+AGG	0.411704	4:-24510429	None:intergenic
CCAGAATAACCACTTAATAT+AGG	0.414414	4:-24511805	None:intergenic
ACTCTATCTCCAGGCTTAGA+AGG	0.416607	4:+24510366	MsaT019180.1:CDS
TGAATTGAATCCAAGGGAAC+TGG	0.417947	4:+24513013	MsaT019180.1:CDS
TCATTCTCCGAACCATCAAC+TGG	0.418732	4:-24514450	None:intergenic
AGAAAAGAACTCGAAATATT+GGG	0.421447	4:+24511986	MsaT019180.1:CDS
TGCTTCTGCAGTTCATGAAC+AGG	0.426089	4:+24514761	MsaT019180.1:CDS
CTTGAATTGCCAACCGGATC+GGG	0.426831	4:+24514545	MsaT019180.1:CDS
TCTCCATACACGTCCACTTA+GGG	0.429073	4:-24512868	None:intergenic
TCTGGTTTGATTTCTCTAGA+AGG	0.431001	4:-24510072	None:intergenic
AACTCTTGATCTGTAATTCT+GGG	0.432750	4:-24510474	None:intergenic
AATGTCACCTCCACTTCTAT+TGG	0.433907	4:+24510302	MsaT019180.1:CDS
GCTTGCATGTCTTCCCGATC+CGG	0.434187	4:-24514558	None:intergenic
ATGTTCCATGCAAAAGAATC+TGG	0.435693	4:+24509171	MsaT019180.1:CDS
CCTGTGTTTCCTTCTAAGCC+TGG	0.440594	4:-24510375	None:intergenic
CCTTGGCAGCATCAGCCACT+TGG	0.441564	4:-24514512	None:intergenic
ATTCTCATAAACACCAGAAT+AGG	0.444115	4:-24511779	None:intergenic
TTCACTTGAATGAGAACCAT+TGG	0.445941	4:-24511752	None:intergenic
TAATGTCCTTGTTGATGAAA+GGG	0.448219	4:+24510269	MsaT019180.1:CDS
CACGATCCAGAGCTGGCCTC+TGG	0.448625	4:-24514094	None:intergenic
TTCTTCTCAGGAACCAGCAC+AGG	0.450915	4:-24511967	None:intergenic
GGCAAGCCAGAGGCCAGCTC+TGG	0.450969	4:+24514088	MsaT019180.1:CDS
CCCATTATAAGTTTCCCCTC+AGG	0.451841	4:-24511603	None:intergenic
TGCTGGTCGAATTGGTCGCT+TGG	0.454425	4:+24510707	MsaT019180.1:CDS
AATGTGAGGGGTATGCAAGT+TGG	0.455328	4:+24509985	MsaT019180.1:CDS
CTGATTCCTTCCTCGTCCTT+CGG	0.456088	4:-24510433	None:intergenic
TTTAACTCCAGTTGATGGTT+CGG	0.456293	4:+24514443	MsaT019180.1:CDS
AAAAGATTATCCAGTTCCCT+TGG	0.457180	4:-24513023	None:intergenic
CCAGAAGTCTTACCCTCTGC+TGG	0.459866	4:+24510090	MsaT019180.1:CDS
ATAATGTCCTTGTTGATGAA+AGG	0.463710	4:+24510268	MsaT019180.1:CDS
GGCCTTCTTCAGTCTCAAAA+GGG	0.464157	4:+24511826	MsaT019180.1:CDS
TGGAAAGAAGAGTATATTTG+TGG	0.465676	4:+24512613	MsaT019180.1:CDS
TGTTGCATGAACGTTGTTTG+TGG	0.466630	4:+24507387	MsaT019180.1:CDS
CAGTTGCATAGACTGAATAC+AGG	0.468777	4:-24511003	None:intergenic
CCTGAGGGGAAACTTATAAT+GGG	0.471341	4:+24511603	MsaT019180.1:CDS
GAGAATATACCTATATTAAG+TGG	0.472374	4:+24511796	MsaT019180.1:CDS
GTGATTCAAATGGTTCATCT+AGG	0.472455	4:+24510148	MsaT019180.1:CDS
AACAAATTCTGCTTCAGTAC+AGG	0.472669	4:+24511638	MsaT019180.1:CDS
AGGCCTCCTGCCGAAGGACG+AGG	0.477953	4:+24510423	MsaT019180.1:CDS
ACATAGATCGGCGAATTCAC+CGG	0.479610	4:-24507454	None:intergenic
GCAAGTGATGCTGGTCGAAT+TGG	0.484028	4:+24510699	MsaT019180.1:CDS
CAAGAGTTGCAGCAAATATC+GGG	0.484696	4:+24510489	MsaT019180.1:CDS
TTCCCAGCAGTGCATATCTC+TGG	0.485291	4:+24509951	MsaT019180.1:CDS
CCTCACAATGTTGATTTGGA+TGG	0.486454	4:+24510048	MsaT019180.1:CDS
GACAGTCCTGAAGAGAATAG+TGG	0.489705	4:+24514786	MsaT019180.1:CDS
TAGCCAGAGATATGCACTGC+TGG	0.491104	4:-24509954	None:intergenic
CGATTCGGTGGCGGAAAGGT+TGG	0.493342	4:+24507421	MsaT019180.1:CDS
TATTGATAGCGAGGATGCAC+TGG	0.493835	4:+24512029	MsaT019180.1:CDS
GTGGAACCTCTACGTCGATT+CGG	0.494921	4:+24507406	MsaT019180.1:CDS
CCAAGTGGCTGATGCTGCCA+AGG	0.498515	4:+24514512	MsaT019180.1:CDS
TTTGGCCAAATAATAATAGC+AGG	0.500753	4:+24510955	MsaT019180.1:CDS
ATCAACAAGGACATTATGAA+AGG	0.502025	4:-24510262	None:intergenic
GAGACATACCTGAAGCAAAA+TGG	0.503082	4:+24514636	MsaT019180.1:CDS
TTCAAGAAAGTGAATTGTGT+TGG	0.503800	4:-24514821	None:intergenic
AAAATGACAGGATGAGGTAC+TGG	0.505312	4:+24510019	MsaT019180.1:CDS
GGCTAACCAATTAAATGTGA+GGG	0.506229	4:+24509972	MsaT019180.1:CDS
TCACTTGAATGAGAACCATT+GGG	0.509159	4:-24511751	None:intergenic
GTTGTCTTTACCACCTGTGC+TGG	0.513012	4:+24511954	MsaT019180.1:CDS
TGCTTCAGGTATGTCTCCAA+CGG	0.513673	4:-24514630	None:intergenic
GCTTCAGGTATGTCTCCAAC+GGG	0.514701	4:-24514629	None:intergenic
CTTCCTCGTCCTTCGGCAGG+AGG	0.514830	4:-24510426	None:intergenic
CAGTCTATGCAACTGCAAGC+TGG	0.514895	4:+24511011	MsaT019180.1:CDS
CCTGGAGATAGAGTTGGCCT+AGG	0.516685	4:-24510357	None:intergenic
TGCAGTAACATTTAGCCGTA+TGG	0.517779	4:+24511578	MsaT019180.1:intron
GGAATTATGCAGAAAATGAC+AGG	0.518137	4:+24510007	MsaT019180.1:CDS
ACTTGAATTGCCAACCGGAT+CGG	0.518323	4:+24514544	MsaT019180.1:CDS
AAATTCAGCTTCAAATGTGC+AGG	0.519112	4:+24514689	MsaT019180.1:CDS
TTCTAGAGAGCGCATTTAGG+TGG	0.520190	4:-24511861	None:intergenic
CCCTCCTATCTCTCAACCTG+AGG	0.521917	4:+24510875	MsaT019180.1:CDS
TAAATGCGCTCTCTAGAAAG+TGG	0.523105	4:+24511866	MsaT019180.1:CDS
ATTAGACTTGAATTGCCAAC+CGG	0.524003	4:+24514539	MsaT019180.1:CDS
GCAAGATATATATGAATCAC+TGG	0.525449	4:+24510194	MsaT019180.1:CDS
CCGATCTATGTGATAAGTGC+GGG	0.532091	4:+24507466	MsaT019180.1:CDS
ATGTAGAAATGCCTGAAAGC+AGG	0.533071	4:+24511920	MsaT019180.1:CDS
AGGCCTTGCTATACGTATCT+GGG	0.533919	4:-24510406	None:intergenic
TCCTCGATCATGACAACACT+TGG	0.535986	4:-24512111	None:intergenic
GAGTGAGGGAAGACAAAATG+AGG	0.539623	4:+24514728	MsaT019180.1:CDS
CTCCCAATGAATTGAATCCA+AGG	0.539749	4:+24513006	MsaT019180.1:CDS
TCAACAAGGACATTATGAAA+GGG	0.542098	4:-24510261	None:intergenic
ATGCAGAAAATGACAGGATG+AGG	0.542862	4:+24510013	MsaT019180.1:CDS
ATGCTTAGTGCAAGTGATGC+TGG	0.543355	4:+24510690	MsaT019180.1:CDS
CCTAGGCCAACTCTATCTCC+AGG	0.543621	4:+24510357	MsaT019180.1:CDS
TTCTCCATACACGTCCACTT+AGG	0.543699	4:-24512869	None:intergenic
CAGTTGGTGCTGACATGGAT+TGG	0.544785	4:+24511893	MsaT019180.1:CDS
ATTCAAGTCTAATTGTCCCT+TGG	0.544992	4:-24514529	None:intergenic
GAAGATTCACGATCCAGAGC+TGG	0.546355	4:-24514101	None:intergenic
ATACGCAAAGGAAGGCCCTC+AGG	0.550975	4:-24510891	None:intergenic
ATTTGGCAAAGGAGCGGTAA+GGG	0.556170	4:-24510238	None:intergenic
CCATTATAAGTTTCCCCTCA+GGG	0.557442	4:-24511602	None:intergenic
AAGTCCTGAAGTACTTGCAC+AGG	0.558364	4:-24509386	None:intergenic
GAACTCAGTTGGTGCTGACA+TGG	0.559385	4:+24511888	MsaT019180.1:CDS
AAGATGTGAAAGGAAAAGAA+TGG	0.562266	4:+24510916	MsaT019180.1:CDS
TTTCCGTCTAATATGCCCAA+TGG	0.563491	4:+24511736	MsaT019180.1:CDS
GATCGGGAAGACATGCAAGC+TGG	0.563741	4:+24514561	MsaT019180.1:CDS
GTTTCCCCTCAGGGTCCATA+CGG	0.563865	4:-24511593	None:intergenic
CTTGGCTTAACTTCTGGAGG+TGG	0.565044	4:-24512093	None:intergenic
GCTGAAACTTACTTGGGAAG+AGG	0.565759	4:+24512053	MsaT019180.1:CDS
AAAGGTTGGATTCTTCGATC+CGG	0.567319	4:+24507435	MsaT019180.1:CDS
GACAAGCAGCGGCAAGCCAG+AGG	0.570055	4:+24514078	MsaT019180.1:CDS
TTCTCAGGAACCAGCACAGG+TGG	0.570575	4:-24511964	None:intergenic
CCTCCTATCTCTCAACCTGA+GGG	0.572708	4:+24510876	MsaT019180.1:CDS
ACGTCGATTCGGTGGCGGAA+AGG	0.573751	4:+24507417	MsaT019180.1:CDS
TCAAATGGTTCATCTAGGAC+TGG	0.575345	4:+24510153	MsaT019180.1:CDS
ACTTATAATGGGGTTCAGGA+AGG	0.575522	4:+24511614	MsaT019180.1:CDS
CCTCTACGTCGATTCGGTGG+CGG	0.575763	4:+24507412	MsaT019180.1:CDS
AGCCAGAGATATGCACTGCT+GGG	0.576349	4:-24509953	None:intergenic
GATTTGGCAAAGGAGCGGTA+AGG	0.576714	4:-24510239	None:intergenic
TCTAGAAAGTGGAACTCAGT+TGG	0.578317	4:+24511877	MsaT019180.1:CDS
GGAGATAGAGTTGGCCTAGG+AGG	0.578335	4:-24510354	None:intergenic
TCTAGAGAGCGCATTTAGGT+GGG	0.579194	4:-24511860	None:intergenic
TCCATGCAAAAGAATCTGGT+TGG	0.579415	4:+24509175	MsaT019180.1:CDS
TTCAAAGAGTGGCACAATGG+TGG	0.580676	4:-24510664	None:intergenic
GCATGTCTTCCCGATCCGGT+TGG	0.581547	4:-24514554	None:intergenic
CCTCAGGTTGAGAGATAGGA+GGG	0.582109	4:-24510875	None:intergenic
TCTAAGCCTGGAGATAGAGT+TGG	0.583408	4:-24510363	None:intergenic
GATCTAGACGCCAATAGAAG+TGG	0.585121	4:-24510312	None:intergenic
TATCTGCAGCGTCTCCATTG+TGG	0.587990	4:+24509304	MsaT019180.1:intron
ATAGCAAGGCCTCCTGCCGA+AGG	0.591462	4:+24510417	MsaT019180.1:CDS
ATACTCTTCTTTCCAAACAC+AGG	0.593392	4:-24512605	None:intergenic
ACACTTGGCTTAACTTCTGG+AGG	0.596735	4:-24512096	None:intergenic
TTATGAAAGGGATTTGGCAA+AGG	0.597001	4:-24510249	None:intergenic
CCCGCACTTATCACATAGAT+CGG	0.598617	4:-24507466	None:intergenic
CCCTCAGGTTGAGAGATAGG+AGG	0.598922	4:-24510876	None:intergenic
ACAGTCCTGAAGAGAATAGT+GGG	0.601508	4:+24514787	MsaT019180.1:CDS
TTCACATCTTGGATACGCAA+AGG	0.602051	4:-24510903	None:intergenic
TCCCAATGAATTGAATCCAA+GGG	0.602257	4:+24513007	MsaT019180.1:CDS
GAGGTTGCTTATTGATAGCG+AGG	0.605174	4:+24512020	MsaT019180.1:CDS
GAACCATTGGGCATATTAGA+CGG	0.606117	4:-24511739	None:intergenic
CATCTTGGATACGCAAAGGA+AGG	0.606572	4:-24510899	None:intergenic
CTCTTCTTTCCAAACACAGG+AGG	0.606920	4:-24512602	None:intergenic
CCATCCAAATCAACATTGTG+AGG	0.607409	4:-24510048	None:intergenic
TGGCTAACCAATTAAATGTG+AGG	0.608102	4:+24509971	MsaT019180.1:CDS
TGAAGAAGCAAATCTCCCGT+TGG	0.612037	4:+24514614	MsaT019180.1:CDS
CTCCATACACGTCCACTTAG+GGG	0.615994	4:-24512867	None:intergenic
TTGCGTATCCAAGATGTGAA+AGG	0.616182	4:+24510906	MsaT019180.1:CDS
TCCATACACGTCCACTTAGG+GGG	0.618210	4:-24512866	None:intergenic
AGAGTGTACTTCATGTGGCA+AGG	0.623087	4:+24509200	MsaT019180.1:CDS
CAAGAAACAAAGACAAGCAG+CGG	0.623503	4:+24514067	MsaT019180.1:CDS
TTTGTTCCCTTTCATCAACA+AGG	0.624287	4:-24510275	None:intergenic
GCTTCCAAATTCTCCAGCAG+AGG	0.626276	4:-24510103	None:intergenic
CTTCCAAATTCTCCAGCAGA+GGG	0.626325	4:-24510102	None:intergenic
ACAGACAACCAATGAGAGTG+AGG	0.631032	4:+24514713	MsaT019180.1:CDS
TTCAAGTAACCAAGACCAAG+TGG	0.631930	4:+24514497	MsaT019180.1:CDS
TGGTTCATCTAGGACTGGCA+AGG	0.632497	4:+24510158	MsaT019180.1:CDS
CTCCTGCCGAAGGACGAGGA+AGG	0.643593	4:+24510427	MsaT019180.1:CDS
CTAGACGCCAATAGAAGTGG+AGG	0.644014	4:-24510309	None:intergenic
CAGCTTGAAATACTTGACAC+TGG	0.646808	4:+24509346	MsaT019180.1:CDS
TTGAAGCAACACATCCACAA+TGG	0.648024	4:-24509318	None:intergenic
GTAATTCTGGGCCAATATCG+CGG	0.648517	4:-24510462	None:intergenic
TATTTGTGGTACGATCTACT+GGG	0.648538	4:+24512627	MsaT019180.1:CDS
GGTTCGGAGAATGAAGCAAG+AGG	0.650245	4:+24514459	MsaT019180.1:CDS
CCGCCACCGAATCGACGTAG+AGG	0.651077	4:-24507412	None:intergenic
GCCAAGTGTTGTCATGATCG+AGG	0.651574	4:+24512110	MsaT019180.1:CDS
AGTTGCATAGACTGAATACA+GGG	0.651610	4:-24511002	None:intergenic
ACTGGAGCTGAAACTTACTT+GGG	0.656896	4:+24512047	MsaT019180.1:CDS
TTTCCCAGATACGTATAGCA+AGG	0.657040	4:+24510403	MsaT019180.1:CDS
AACTGGAGTTAAATGTCGAG+AGG	0.658373	4:-24514433	None:intergenic
CTTAGAAGGAAACACAGGCA+TGG	0.658844	4:+24510380	MsaT019180.1:CDS
CCAGGCTTAGAAGGAAACAC+AGG	0.660663	4:+24510375	MsaT019180.1:CDS
GCTAACCAATTAAATGTGAG+GGG	0.663001	4:+24509973	MsaT019180.1:CDS
CAAGTGGCTGATGCTGCCAA+GGG	0.663194	4:+24514513	MsaT019180.1:CDS
ACCACAGGATAAGTGAGCAG+TGG	0.664291	4:+24512789	MsaT019180.1:intron
GAACCTCTACGTCGATTCGG+TGG	0.665625	4:+24507409	MsaT019180.1:CDS
CTGAGGGGAAACTTATAATG+GGG	0.667153	4:+24511604	MsaT019180.1:CDS
GCCGATCTATGTGATAAGTG+CGG	0.667157	4:+24507465	MsaT019180.1:CDS
TCAGCAATCATGGAGGACCA+AGG	0.667770	4:+24514389	MsaT019180.1:CDS
TTTAGCCGTATGGACCCTGA+GGG	0.672269	4:+24511588	MsaT019180.1:CDS
AAAGGGATTTGGCAAAGGAG+CGG	0.674141	4:-24510244	None:intergenic
TGATGCTTTAGCGAATGCTG+CGG	0.684565	4:+24514130	MsaT019180.1:CDS
GTTCGGAGAATGAAGCAAGA+GGG	0.686630	4:+24514460	MsaT019180.1:CDS
CAGACAACCAATGAGAGTGA+GGG	0.686644	4:+24514714	MsaT019180.1:CDS
ATTTAGCCGTATGGACCCTG+AGG	0.687200	4:+24511587	MsaT019180.1:CDS
TTGCATAGACTGAATACAGG+GGG	0.707100	4:-24511000	None:intergenic
TGGAGAGAGTGTACTTCATG+TGG	0.711681	4:+24509195	MsaT019180.1:CDS
CTTGAAATACTTGACACTGG+TGG	0.733966	4:+24509349	MsaT019180.1:CDS
TTAGCCGTATGGACCCTGAG+GGG	0.752430	4:+24511589	MsaT019180.1:CDS
GTTGCATAGACTGAATACAG+GGG	0.770443	4:-24511001	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	24507375	24514842	24507375	ID=MsaG019180
Chr4	mRNA	24507375	24514842	24507375	ID=MsaT019180.1;Parent=MsaG019180
Chr4	exon	24507375	24507487	24507375	ID=MsaT019180.1.exon1;Parent=MsaT019180.1
Chr4	CDS	24507375	24507487	24507375	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24509140	24509221	24509140	ID=MsaT019180.1.exon2;Parent=MsaT019180.1
Chr4	CDS	24509140	24509221	24509140	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24509313	24509414	24509313	ID=MsaT019180.1.exon3;Parent=MsaT019180.1
Chr4	CDS	24509313	24509414	24509313	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24509895	24510514	24509895	ID=MsaT019180.1.exon4;Parent=MsaT019180.1
Chr4	CDS	24509895	24510514	24509895	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24510656	24510752	24510656	ID=MsaT019180.1.exon5;Parent=MsaT019180.1
Chr4	CDS	24510656	24510752	24510656	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24510867	24511041	24510867	ID=MsaT019180.1.exon6;Parent=MsaT019180.1
Chr4	CDS	24510867	24511041	24510867	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24511583	24511659	24511583	ID=MsaT019180.1.exon7;Parent=MsaT019180.1
Chr4	CDS	24511583	24511659	24511583	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24511730	24512155	24511730	ID=MsaT019180.1.exon8;Parent=MsaT019180.1
Chr4	CDS	24511730	24512155	24511730	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24512599	24512648	24512599	ID=MsaT019180.1.exon9;Parent=MsaT019180.1
Chr4	CDS	24512599	24512648	24512599	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24512796	24512909	24512796	ID=MsaT019180.1.exon10;Parent=MsaT019180.1
Chr4	CDS	24512796	24512909	24512796	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24512992	24513059	24512992	ID=MsaT019180.1.exon11;Parent=MsaT019180.1
Chr4	CDS	24512992	24513059	24512992	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24514063	24514170	24514063	ID=MsaT019180.1.exon12;Parent=MsaT019180.1
Chr4	CDS	24514063	24514170	24514063	ID=cds.MsaT019180.1;Parent=MsaT019180.1
Chr4	exon	24514346	24514842	24514346	ID=MsaT019180.1.exon13;Parent=MsaT019180.1
Chr4	CDS	24514346	24514842	24514346	ID=cds.MsaT019180.1;Parent=MsaT019180.1

Gene Sequence

>MsaT019180.1

ATGGAGTCAAAGTGTTGCATGAACGTTGTTTGTGGAACCTCTACGTCGATTCGGTGGCGGAAAGGTTGGATTCTTCGATCCGGTGAATTCGCCGATCTATGTGATAAGTGCGGTTCTGCTTATGAGCAAACAGCTTTTTGCGATATGTTCCATGCAAAAGAATCTGGTTGGAGAGAGTGTACTTCATGTGGCAAGCGTCTCCATTGTGGATGTGTTGCTTCAAAGTCTCAGCTTGAAATACTTGACACTGGTGGTGTAAGCTGTATTACCTGTGCAAGTACTTCAGGACTTCAACCTATTGCTAGTAATGAAAAGCCTAATGAATCTGGAACAGCAAAAGTAAATAATGTCAGTTCCCAGCAGTGCATATCTCTGGCTAACCAATTAAATGTGAGGGGTATGCAAGTTGGGAATTATGCAGAAAATGACAGGATGAGGTACTGGCTCAAACCTCACAATGTTGATTTGGATGGACCTTCTAGAGAAATCAAACCAGAAGTCTTACCCTCTGCTGGAGAATTTGGAAGCACTTTGATGTCACAATTTCATCGTGATTCAAATGGTTCATCTAGGACTGGCAAGGCAGAGAATGACATGCAAGATATATATGAATCACTGGCACAGACAAATTTGAGTATGACCCTTACCGCTCCTTTGCCAAATCCCTTTCATAATGTCCTTGTTGATGAAAGGGAACAAAGCAAAATGTCACCTCCACTTCTATTGGCGTCTAGATCTCGTCATCTTTTACCTAAACCTCCTAGGCCAACTCTATCTCCAGGCTTAGAAGGAAACACAGGCATGGTTTCCCAGATACGTATAGCAAGGCCTCCTGCCGAAGGACGAGGAAGGAATCAGTTGCTTCCGCGATATTGGCCCAGAATTACAGATCAAGAGTTGCAGCAAATATCGGGAGATTCAAATTCCACCATTGTGCCACTCTTTGAAAAGATGCTTAGTGCAAGTGATGCTGGTCGAATTGGTCGCTTGGTTCTTCCAAAAGCATGTGCTGAAGCATATTTCCCTCCTATCTCTCAACCTGAGGGCCTTCCTTTGCGTATCCAAGATGTGAAAGGAAAAGAATGGATGTTTCAATTTAGATTTTGGCCAAATAATAATAGCAGGATGTATGTTTTAGAAGGGGTAACCCCCTGTATTCAGTCTATGCAACTGCAAGCTGGCGATACTGTAACATTTAGCCGTATGGACCCTGAGGGGAAACTTATAATGGGGTTCAGGAAGGCAACAAATTCTGCTTCAGTACAGGAAACTTTTCCGTCTAATATGCCCAATGGTTCTCATTCAAGTGAAACTTCCTATTCTGGTGTTTATGAGAATATACCTATATTAAGTGGTTATTCTGGCCTTCTTCAGTCTCAAAAGGGTTGTTCAGAAACCCACCTAAATGCGCTCTCTAGAAAGTGGAACTCAGTTGGTGCTGACATGGATTGGCATAATGTAGAAATGCCTGAAAGCAGGAAAAGAGATGTGTTGTCTTTACCACCTGTGCTGGTTCCTGAGAAGAAAAGAACTCGAAATATTGGGTCAAAAAGTAAGAGGTTGCTTATTGATAGCGAGGATGCACTGGAGCTGAAACTTACTTGGGAAGAGGCTCAGGATTTACTTCGTCCACCTCCAGAAGTTAAGCCAAGTGTTGTCATGATCGAGGATCACTTATTTGAAGAGTATGAAGAGCCTCCTGTGTTTGGAAAGAAGAGTATATTTGTGGTACGATCTACTGGGATAAGTGAGCAGTGGACACAGTGTGATAGTTGCCAAAAATGGCGGAAGTTACCGGTTGATGTACTTATTCCCCCTAAGTGGACGTGTATGGAGAACTTTTGGGATCAAAGCAGGTGTTCATGTGCTGCTCCCAATGAATTGAATCCAAGGGAACTGGATAATCTTTTGAGAATGAACAATGAATTCAAGAAACAAAGACAAGCAGCGGCAAGCCAGAGGCCAGCTCTGGATCGTGAATCTTCTGGTCTTGATGCTTTAGCGAATGCTGCGGTACTTGGAGATGATGCAAAAAAGCGTCAATCTGAGCGCGAAGCTGAGATTGCTCAGAAAAATCAGCAATCATGGAGGACCAAGGATGAATCAGAAGTAGACAGCACCTCTCGACATTTAACTCCAGTTGATGGTTCGGAGAATGAAGCAAGAGGGCCAAATGAATTAGATTCAAGTAACCAAGACCAAGTGGCTGATGCTGCCAAGGGACAATTAGACTTGAATTGCCAACCGGATCGGGAAGACATGCAAGCTGGACCAAACACTCTGAGCATGATGACTCTTCTTGAAGAAGCAAATCTCCCGTTGGAGACATACCTGAAGCAAAATGGTCTTTCTAGCTTGATATCAGAGCAGCAAACAAATTCAGCTTCAAATGTGCAGGCACAGACAACCAATGAGAGTGAGGGAAGACAAAATGAGGATTGTTGTACTGCTTCTGCAGTTCATGAACAGGAAGACAGTCCTGAAGAGAATAGTGGGCAAGACAGAGACCAACACAATTCACTTTCTTGA

Protein sequence

>MsaT019180.1

MESKCCMNVVCGTSTSIRWRKGWILRSGEFADLCDKCGSAYEQTAFCDMFHAKESGWRECTSCGKRLHCGCVASKSQLEILDTGGVSCITCASTSGLQPIASNEKPNESGTAKVNNVSSQQCISLANQLNVRGMQVGNYAENDRMRYWLKPHNVDLDGPSREIKPEVLPSAGEFGSTLMSQFHRDSNGSSRTGKAENDMQDIYESLAQTNLSMTLTAPLPNPFHNVLVDEREQSKMSPPLLLASRSRHLLPKPPRPTLSPGLEGNTGMVSQIRIARPPAEGRGRNQLLPRYWPRITDQELQQISGDSNSTIVPLFEKMLSASDAGRIGRLVLPKACAEAYFPPISQPEGLPLRIQDVKGKEWMFQFRFWPNNNSRMYVLEGVTPCIQSMQLQAGDTVTFSRMDPEGKLIMGFRKATNSASVQETFPSNMPNGSHSSETSYSGVYENIPILSGYSGLLQSQKGCSETHLNALSRKWNSVGADMDWHNVEMPESRKRDVLSLPPVLVPEKKRTRNIGSKSKRLLIDSEDALELKLTWEEAQDLLRPPPEVKPSVVMIEDHLFEEYEEPPVFGKKSIFVVRSTGISEQWTQCDSCQKWRKLPVDVLIPPKWTCMENFWDQSRCSCAAPNELNPRELDNLLRMNNEFKKQRQAAASQRPALDRESSGLDALANAAVLGDDAKKRQSEREAEIAQKNQQSWRTKDESEVDSTSRHLTPVDGSENEARGPNELDSSNQDQVADAAKGQLDLNCQPDREDMQAGPNTLSMMTLLEEANLPLETYLKQNGLSSLISEQQTNSASNVQAQTTNESEGRQNEDCCTASAVHEQEDSPEENSGQDRDQHNSLS*