AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019205

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019205 MtrunA17_Chr1g0203051 42.667 150 86 0 1 150 64 213 1.09e-39 141
MsaG019205 MtrunA17_Chr8g0389811 44.094 127 71 0 1 127 69 195 3.21e-33 117
MsaG019205 MtrunA17_Chr1g0171741 40.559 143 84 1 3 145 120 261 9.39e-32 114
MsaG019205 MtrunA17_Chr2g0331371 49.167 120 61 0 1 120 40 159 2.08e-31 112
MsaG019205 MtrunA17_Chr6g0473421 47.826 92 48 0 1 92 40 131 1.13e-26 98.6
MsaG019205 MtrunA17_Chr2g0287651 34.028 144 95 0 7 150 66 209 1.02e-23 96.7
MsaG019205 MtrunA17_Chr1g0172931 38.776 98 60 0 1 98 61 158 3.93e-18 76.6
MsaG019205 MtrunA17_Chr2g0276841 35.200 125 76 4 22 142 76 199 8.35e-15 70.9
MsaG019205 MtrunA17_Chr2g0290391 37.398 123 74 2 22 142 57 178 2.75e-14 69.7
MsaG019205 MtrunA17_Chr1g0202341 30.667 150 98 3 2 147 34 181 3.95e-14 68.9
MsaG019205 MtrunA17_Chr1g0198761 33.858 127 72 4 22 138 53 177 8.59e-13 65.1
MsaG019205 MtrunA17_Chr6g0458391 35.433 127 71 4 21 143 96 215 9.65e-13 65.1
MsaG019205 MtrunA17_Chr5g0448761 31.410 156 103 3 14 165 58 213 2.92e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019205 AT5G28530.5 38.211 123 73 2 22 142 61 182 1.17e-19 85.5
MsaG019205 AT5G28530.4 38.211 123 73 2 22 142 61 182 1.21e-19 85.5
MsaG019205 AT5G28530.3 38.211 123 73 2 22 142 61 182 1.36e-19 85.1
MsaG019205 AT5G28530.2 38.211 123 73 2 22 142 61 182 1.36e-19 85.1
MsaG019205 AT5G28530.1 38.211 123 73 2 22 142 61 182 1.41e-19 85.1
MsaG019205 AT1G10240.2 35.338 133 81 4 22 150 54 185 2.13e-15 73.2
MsaG019205 AT1G10240.1 35.338 133 81 4 22 150 54 185 2.13e-15 73.2

Find 36 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGTTCATGTCAAGGATTC+AGG 0.195852 4:+24912554 MsaT019205.1:CDS
GGCCTTTGGCAAATGCTTCC+AGG 0.280849 4:+24912834 MsaT019205.1:CDS
GATCACAACCATGATTTGTT+AGG 0.323090 4:+24912709 MsaT019205.1:CDS
GCTTTGACAAAATATCTAAA+TGG 0.330891 4:-24912660 None:intergenic
TTGCCAAAGGCCCGTACATT+TGG 0.334491 4:-24912823 None:intergenic
TTACCAAATGTACGGGCCTT+TGG 0.368921 4:+24912820 MsaT019205.1:CDS
TTGTTAGGTGGTACTCTTTG+TGG 0.391770 4:+24912724 MsaT019205.1:CDS
CTCTTGGTTTGCTAGAACGT+CGG 0.402576 4:+24912465 MsaT019205.1:CDS
TTCGTAAGGGCCAAGTGGTT+AGG 0.412561 4:+24912497 MsaT019205.1:CDS
GTCAAAGCATTGGTATGTTA+CGG 0.415976 4:+24912675 MsaT019205.1:CDS
GTGGAGAGATATGAATTTGT+TGG 0.419123 4:+24912418 MsaT019205.1:CDS
GCCTTTGGCAAATGCTTCCA+GGG 0.430234 4:+24912835 MsaT019205.1:CDS
TCGTAAGGGCCAAGTGGTTA+GGG 0.458849 4:+24912498 MsaT019205.1:CDS
CTCGCGCTTTCGGGTTTCCA+TGG 0.462637 4:-24912593 None:intergenic
TCTTGGTTTGCTAGAACGTC+GGG 0.465242 4:+24912466 MsaT019205.1:CDS
TAGCAAACCAAGAGTAGAAA+AGG 0.470858 4:-24912456 None:intergenic
ACCTTTGTTTGTTCATGTCA+AGG 0.481061 4:+24912547 MsaT019205.1:CDS
AAGGATTCAGGGAGGATATG+AGG 0.506588 4:+24912566 MsaT019205.1:CDS
TTGTTCATGTCAAGGATTCA+GGG 0.511148 4:+24912555 MsaT019205.1:CDS
GAGTACCACCTAACAAATCA+TGG 0.528267 4:-24912717 None:intergenic
ATAAGATCTTACCAAATGTA+CGG 0.534016 4:+24912812 MsaT019205.1:CDS
TGATATAATGAAGATTGACT+TGG 0.539341 4:+24912375 MsaT019205.1:CDS
TCATACCTTATGAAAACCCC+TGG 0.544957 4:-24912852 None:intergenic
CACAACCATGATTTGTTAGG+TGG 0.552454 4:+24912712 MsaT019205.1:CDS
CATTCCGATCCCTAACCACT+TGG 0.561972 4:-24912507 None:intergenic
ACGATCAATTTCAGCTATGT+CGG 0.566913 4:-24912779 None:intergenic
TAAGATCTTACCAAATGTAC+GGG 0.570318 4:+24912813 MsaT019205.1:CDS
CCTTTGGCAAATGCTTCCAG+GGG 0.590967 4:+24912836 MsaT019205.1:CDS
AGGGCCAAGTGGTTAGGGAT+CGG 0.593499 4:+24912503 MsaT019205.1:CDS
TCCTTGACATGAACAAACAA+AGG 0.611453 4:-24912548 None:intergenic
CCCCTGGAAGCATTTGCCAA+AGG 0.641374 4:-24912836 None:intergenic
GGAGGATATGAGGTTAACCA+TGG 0.644884 4:+24912576 MsaT019205.1:CDS
TTCAGTTCGTAAGGGCCAAG+TGG 0.644951 4:+24912492 MsaT019205.1:CDS
TGAAATTGATCGTGTCAGCG+AGG 0.708893 4:+24912789 MsaT019205.1:CDS
TTCATGTCAAGGATTCAGGG+AGG 0.717283 4:+24912558 MsaT019205.1:CDS
CAAGTGGTTAGGGATCGGAA+TGG 0.750885 4:+24912508 MsaT019205.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 24912370 24912867 24912370 ID=MsaG019205
Chr4 mRNA 24912370 24912867 24912370 ID=MsaT019205.1;Parent=MsaG019205
Chr4 exon 24912370 24912867 24912370 ID=MsaT019205.1.exon1;Parent=MsaT019205.1
Chr4 CDS 24912370 24912867 24912370 ID=cds.MsaT019205.1;Parent=MsaT019205.1
Gene Sequence

>MsaT019205.1

ATGGTTGATATAATGAAGATTGACTTGGAAAAATTTTGCTTGGCTAATGTGGAGAGATATGAATTTGTTGGATTAGATGTTGCTTACCTTTTCTACTCTTGGTTTGCTAGAACGTCGGGATTTTCAGTTCGTAAGGGCCAAGTGGTTAGGGATCGGAATGGTGAAGTAGTTCAGCAAACCTTTGTTTGTTCATGTCAAGGATTCAGGGAGGATATGAGGTTAACCATGGAAACCCGAAAGCGCGAGCCAAAAAATGAAACAAGATGTGGGTGTAATGCAAAATTTCGAGTCCATTTAGATATTTTGTCAAAGCATTGGTATGTTACGGTATTTGAGTTTGATCACAACCATGATTTGTTAGGTGGTACTCTTTGTGGAATGTTGCCAGCGTACAGAAAAATGAGCGAGGCCGACATAGCTGAAATTGATCGTGTCAGCGAGGATAAGATCTTACCAAATGTACGGGCCTTTGGCAAATGCTTCCAGGGGTTTTCATAA
Protein sequence

>MsaT019205.1

MVDIMKIDLEKFCLANVERYEFVGLDVAYLFYSWFARTSGFSVRKGQVVRDRNGEVVQQTFVCSCQGFREDMRLTMETRKREPKNETRCGCNAKFRVHLDILSKHWYVTVFEFDHNHDLLGGTLCGMLPAYRKMSEADIAEIDRVSEDKILPNVRAFGKCFQGFS*