AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019281

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019281 MtrunA17_Chr4g0013741 97.094 929 26 1 1 928 1 929 0.0 1859
MsaG019281 MtrunA17_Chr6g0485441 22.593 509 334 11 96 585 58 525 6.86e-24 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019281 AT1G22870.1 65.823 948 268 13 1 927 1 913 0.0 1224
MsaG019281 AT1G71410.1 64.957 936 292 13 1 927 1 909 0.0 1193
MsaG019281 AT1G71410.2 64.957 936 292 13 1 927 1 909 0.0 1193
MsaG019281 AT1G22870.4 68.496 838 224 8 1 827 1 809 0.0 1176
MsaG019281 AT1G22870.3 62.835 783 235 13 166 927 1 748 0.0 944
MsaG019281 AT1G22870.2 61.613 719 220 13 230 927 9 692 0.0 832
MsaG019281 AT2G40730.1 22.528 617 394 17 40 630 18 576 3.29e-20 97.1

Find 245 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATAGACTCCACAGAATTT+TGG 0.154681 4:+26070297 None:intergenic
CTTGACAAAGAGCATATATT+TGG 0.211975 4:+26071063 None:intergenic
CAAAATAATTTGCCCTTATC+TGG 0.217176 4:-26070641 MsaT019281.1:CDS
AATTCCAACAATGGTATTTC+AGG 0.220096 4:-26070410 MsaT019281.1:CDS
TCTCTTCAAATGAATCTTTC+AGG 0.221457 4:-26077528 MsaT019281.1:CDS
CACTGAGCAGCCACCCCTTT+TGG 0.226191 4:-26069985 MsaT019281.1:CDS
CGTTTGGGTGTTGGATAAAA+AGG 0.235354 4:-26082635 MsaT019281.1:CDS
CTTCTCTTGGGATGCAGATT+GGG 0.235573 4:-26070762 MsaT019281.1:CDS
TCTGGTCTTACCTCACAATT+TGG 0.249950 4:-26070551 MsaT019281.1:CDS
GTTACGTGCTCTGCGTTTCC+TGG 0.252212 4:-26075687 MsaT019281.1:CDS
TAGATGTATTAATAGAATTC+TGG 0.257450 4:+26070708 None:intergenic
GCCTGGAAACTTGGTGGATT+TGG 0.258496 4:-26080858 MsaT019281.1:CDS
GGAGTTGCTGTGACTGATTC+TGG 0.265135 4:-26070878 MsaT019281.1:CDS
CACCTGGGTATCAAGTTCTT+TGG 0.281777 4:+26073610 None:intergenic
TGGCCGCCTCGTGCATCTTC+TGG 0.283391 4:-26070569 MsaT019281.1:CDS
AAAGGGCGGGGAAGAGGCAG+AGG 0.302195 4:-26070047 MsaT019281.1:CDS
GAAGAACATGTGAAATTAAA+TGG 0.302392 4:+26073708 None:intergenic
AGTATCCGGTTGTTTGCGTT+TGG 0.312856 4:-26082651 MsaT019281.1:CDS
TTTGAACCTCAGAGTTCTCT+TGG 0.315441 4:-26070224 MsaT019281.1:CDS
AAGTGTTCCTAAGGATCTTA+AGG 0.324960 4:-26082392 MsaT019281.1:CDS
CTCAGGAGCAGTGTAATTAA+TGG 0.336076 4:+26078382 None:intergenic
ATCTACTGAAGGACATGAAT+TGG 0.341032 4:+26070598 None:intergenic
CCTTTACCCACTGTGGTTGA+AGG 0.348847 4:+26070065 None:intergenic
CAAAATTCTGTGGAGTCTAT+TGG 0.350860 4:-26070296 MsaT019281.1:CDS
ACAAGCAATTTAAGCAATCT+TGG 0.353361 4:-26070353 MsaT019281.1:CDS
GCTTCTCTTGGGATGCAGAT+TGG 0.355593 4:-26070763 MsaT019281.1:CDS
CCGAGACGGATCGCGTGATT+TGG 0.361097 4:+26082681 None:intergenic
AGGATCGTCATCTTCAATAA+TGG 0.361466 4:+26070484 None:intergenic
TCCTGCAGTTAACGGCCTTC+AGG 0.362743 4:-26070834 MsaT019281.1:CDS
GAGAGCATCCTTTGCAAGTC+AGG 0.364314 4:+26077547 None:intergenic
GAATAGTTTGCAAGATTTCC+AGG 0.366270 4:+26071358 None:intergenic
CAACCCTTATGTGTACCCTT+GGG 0.366688 4:-26071305 MsaT019281.1:CDS
CTTGGCAGACCAATTGTTGC+TGG 0.367793 4:+26070322 None:intergenic
TTTGCAGGAAATGGGATTGT+TGG 0.371651 4:-26081246 MsaT019281.1:intron
TTCATTAATTTGCAGGAAAT+GGG 0.374196 4:-26081254 MsaT019281.1:intron
AAGGCTTGCGAGCAATTAAA+TGG 0.376579 4:+26078291 None:intergenic
CTCATCTATCCCATCGGATC+TGG 0.376874 4:-26077572 MsaT019281.1:CDS
AATGTTAAATCAACTGATCT+TGG 0.377809 4:-26070143 MsaT019281.1:CDS
ACCAAATCCACCAAGTTTCC+AGG 0.381691 4:+26080857 None:intergenic
GCTTCCGTTGCGAATACTCT+TGG 0.382944 4:-26082433 MsaT019281.1:CDS
TTGGTGGGGCCCGTTGCTTT+AGG 0.387441 4:+26070737 None:intergenic
AGGAGCACCACTTCTTCAGC+TGG 0.387774 4:-26078353 MsaT019281.1:CDS
CGCCCTAGTGGTTCATTATC+CGG 0.389618 4:-26070443 MsaT019281.1:CDS
GTATCCGGTTGTTTGCGTTT+GGG 0.392102 4:-26082650 MsaT019281.1:CDS
CTGATTAGATACACCAGATA+AGG 0.392866 4:+26070628 None:intergenic
CAGACCAATTGTTGCTGGCC+TGG 0.396129 4:+26070327 None:intergenic
CCGCCGGTCCCGCTTTAGCC+TGG 0.399395 4:-26082717 MsaT019281.1:CDS
TTCTTGAACGGTTGTTGAAA+CGG 0.402267 4:+26082792 None:intergenic
ACATCTAATAAATCTGTGAT+AGG 0.404562 4:-26070692 MsaT019281.1:CDS
GGTTCATTATCCGGTGCATC+TGG 0.404706 4:-26070434 MsaT019281.1:CDS
GTCTCCAGGCCAGCAACAAT+TGG 0.407124 4:-26070331 MsaT019281.1:CDS
TGCAAGTTGTTCATTCTTGT+TGG 0.408006 4:+26070106 None:intergenic
CAACCCCAAGGGTACACATA+AGG 0.408326 4:+26071301 None:intergenic
AATAGAATTCTGGACAGAAT+TGG 0.409022 4:+26070718 None:intergenic
GTGGGTAAAGGGCGGGGAAG+AGG 0.410608 4:-26070053 MsaT019281.1:CDS
CATTCCTGAAATACCATTGT+TGG 0.413881 4:+26070406 None:intergenic
GGAATGGCATTAAACAAGAT+AGG 0.419727 4:-26070389 MsaT019281.1:CDS
ATAGATGAGCTAGCATTCCT+TGG 0.421853 4:+26070266 None:intergenic
TTTAACCATTGCAGAGTCTC+AGG 0.422067 4:-26074588 MsaT019281.1:intron
TACCTTGTTTATTATTAGCT+CGG 0.424935 4:+26073841 None:intergenic
CCTGTCTTAAGCACTGCTTC+TGG 0.425601 4:-26073893 MsaT019281.1:CDS
ACGTGAATCATTATCATCAT+AGG 0.429078 4:+26073670 None:intergenic
ATGTTCCACTCACAGCATAA+TGG 0.430542 4:+26080784 None:intergenic
TCGTAGTCTTGAAGAGGCTT+TGG 0.431079 4:+26082760 None:intergenic
CTTCAACCACAGTGGGTAAA+GGG 0.431127 4:-26070064 MsaT019281.1:CDS
GGGATTGTGCATGTTGTTCA+AGG 0.431730 4:-26082505 MsaT019281.1:CDS
TCATAGGCTCGAACAATCAT+TGG 0.434291 4:+26073686 None:intergenic
AACACCCAAACGCAAACAAC+CGG 0.436701 4:+26082646 None:intergenic
GAGGTTCAAAGCCATTGTTC+AGG 0.439568 4:+26070237 None:intergenic
CTTCCGTTGCGAATACTCTT+GGG 0.441569 4:-26082432 MsaT019281.1:CDS
TGGGTTCTTGAAACAAAGTC+AGG 0.443828 4:-26070204 MsaT019281.1:CDS
TTGAACCTCAGAGTTCTCTT+GGG 0.445956 4:-26070223 MsaT019281.1:CDS
CCAACCCTTATGTGTACCCT+TGG 0.446020 4:-26071306 MsaT019281.1:CDS
ATGGTTAGGTTGAGACATCC+TGG 0.449776 4:-26082526 MsaT019281.1:CDS
CTTGGTATGAGAGTGCTGTC+TGG 0.450433 4:+26070013 None:intergenic
TAAAAGCATTGTCAGATATG+TGG 0.451916 4:-26075526 MsaT019281.1:CDS
TTCAATAATGGATGTGGATG+AGG 0.457716 4:+26070496 None:intergenic
ACGGTGCTGCTGGTTGTCCT+TGG 0.458909 4:+26070803 None:intergenic
AAACTATCTCCTGCAGTTAA+CGG 0.459043 4:-26070842 MsaT019281.1:CDS
TGGATGCTGGGAAGATGGTT+AGG 0.459231 4:-26082540 MsaT019281.1:CDS
TTTGGCGGAGTAAAGCTTCC+AGG 0.460959 4:+26082699 None:intergenic
CATTCAGCTGTTGAGCAGTC+AGG 0.461966 4:+26071098 None:intergenic
CTTAAGACAGGGACAAGGGC+TGG 0.463233 4:+26073905 None:intergenic
CGGGACCGGCGGAACCGATC+TGG 0.463244 4:+26082728 None:intergenic
CCTGTAAAATCCATTGCACT+TGG 0.463536 4:+26077505 None:intergenic
GGTTGTTTGCGTTTGGGTGT+TGG 0.463872 4:-26082644 MsaT019281.1:CDS
GATTGGGGTCCTAAAGCAAC+GGG 0.466852 4:-26070746 MsaT019281.1:CDS
ACAACCGTTCAAGAAGTAAC+AGG 0.467422 4:-26082784 MsaT019281.1:CDS
CTTTACCCACTGTGGTTGAA+GGG 0.467929 4:+26070066 None:intergenic
ACCATTCCCTTAAGATCCTT+AGG 0.469358 4:+26082385 None:intergenic
TTCTTACAAGCATGTGGTCC+AGG 0.474406 4:+26075669 None:intergenic
CTTCCAGGCTAAAGCGGGAC+CGG 0.475226 4:+26082714 None:intergenic
CACCACTTTGTGCAGAACTT+AGG 0.475258 4:-26074646 MsaT019281.1:CDS
AAAAGCATCCTCAGCCGCCT+TGG 0.481850 4:+26082579 None:intergenic
TTTAAGCAATCTTGGTCTCC+AGG 0.483745 4:-26070345 MsaT019281.1:CDS
TGGACCTGTTACTTCTTGAA+CGG 0.486566 4:+26082780 None:intergenic
CCTTCAACCACAGTGGGTAA+AGG 0.490462 4:-26070065 MsaT019281.1:CDS
GAATAAGATGGGCATGGTTG+TGG 0.490924 4:+26081177 None:intergenic
TGTGCATGTTGTTCAAGGGT+TGG 0.491272 4:-26082500 MsaT019281.1:CDS
GTGGTGCTCCTCACCATCTC+AGG 0.494154 4:+26078365 None:intergenic
AGTGTTCCTAAGGATCTTAA+GGG 0.495954 4:-26082391 MsaT019281.1:CDS
AACCCCAAGGGTACACATAA+GGG 0.498164 4:+26071302 None:intergenic
TTCTCTTGGGATGCAGATTG+GGG 0.498334 4:-26070761 MsaT019281.1:CDS
GTTACCCCAACAAATCCAAA+AGG 0.498720 4:+26069970 None:intergenic
CAACAATGGTATTTCAGGAA+TGG 0.499806 4:-26070405 MsaT019281.1:CDS
GATTTGATTAGGATGGATGC+TGG 0.499807 4:-26082553 MsaT019281.1:CDS
TTATCACATTGAGTGGAGCC+TGG 0.500197 4:-26080875 MsaT019281.1:CDS
ACCTGAAGGCCGTTAACTGC+AGG 0.504180 4:+26070833 None:intergenic
TGATTCACGTTTACAAGAAG+AGG 0.505008 4:-26073657 MsaT019281.1:CDS
TGGATTGACCAAGGCGGCTG+AGG 0.505029 4:-26082587 MsaT019281.1:CDS
TGGCTGCTCAGTGCTTGACT+TGG 0.506533 4:+26069995 None:intergenic
TGTTGAAGCAACGGTGCTGC+TGG 0.506869 4:+26070793 None:intergenic
ACCACAGTGGGTAAAGGGCG+GGG 0.507597 4:-26070059 MsaT019281.1:CDS
TCCTTGGAGCTTCCACCTGA+AGG 0.508716 4:+26070819 None:intergenic
ATCTTTCAGGCCAAGTGCAA+TGG 0.508729 4:-26077515 MsaT019281.1:CDS
AACCACAGTGGGTAAAGGGC+GGG 0.509852 4:-26070060 MsaT019281.1:CDS
CTCCACCAGATCGGTTCCGC+CGG 0.511316 4:-26082733 MsaT019281.1:CDS
GTCATCTTCAATAATGGATG+TGG 0.511373 4:+26070490 None:intergenic
TGATTAGATACACCAGATAA+GGG 0.512264 4:+26070629 None:intergenic
GTCTCGGCAGCATCAGTATC+CGG 0.513495 4:-26082665 MsaT019281.1:CDS
TTGAGTGGAGCCTGGAAACT+TGG 0.514583 4:-26080867 MsaT019281.1:CDS
AGACGGATCGCGTGATTTGG+CGG 0.516807 4:+26082684 None:intergenic
TTCCCGTGTATTACGATACA+AGG 0.517523 4:-26075492 MsaT019281.1:intron
CACAATTTGGTGATACTGAA+AGG 0.517575 4:-26070538 MsaT019281.1:CDS
ACTTGATAAACATGCTGTCC+TGG 0.519009 4:-26071376 MsaT019281.1:CDS
TCCTAAGGATCTTAAGGGAA+TGG 0.524386 4:-26082386 MsaT019281.1:intron
CGTGGAGGCCAATCAGCAAA+AGG 0.525518 4:+26070464 None:intergenic
AAAACCATTGGTAGAATCAT+CGG 0.526198 4:+26074608 None:intergenic
GCAGGAAATGGGATTGTTGG+AGG 0.526955 4:-26081243 MsaT019281.1:intron
GTTGTCGGAGGCGAGATTGA+AGG 0.527063 4:-26082611 MsaT019281.1:CDS
TAGATGAGCTAGCATTCCTT+GGG 0.527101 4:+26070267 None:intergenic
TAGGATGGATGCTGGGAAGA+TGG 0.527113 4:-26082545 MsaT019281.1:CDS
GTCAGGAGTGGCATAAGCAA+TGG 0.528064 4:+26071115 None:intergenic
TAATGTTCAAAGTGTTCCTA+AGG 0.530222 4:-26082401 MsaT019281.1:CDS
ACTTCAGATTGCGGAGTCGT+TGG 0.532035 4:-26081207 MsaT019281.1:CDS
CCAGAAGCAGTGCTTAAGAC+AGG 0.532674 4:+26073893 None:intergenic
ATGGATGTTACTTGACCCAC+AGG 0.532744 4:+26070665 None:intergenic
GGGTATCAAGTTCTTTGGCA+AGG 0.533522 4:+26073615 None:intergenic
GTCGGTTGACCATGTCTCCT+AGG 0.533647 4:+26071397 None:intergenic
TCTGCAATGGTTAAAACCAT+TGG 0.534089 4:+26074596 None:intergenic
ACAACCGATGATTCTACCAA+TGG 0.534149 4:-26074612 MsaT019281.1:CDS
AGTCTATTGGTTTGAACCCA+AGG 0.535666 4:-26070283 MsaT019281.1:CDS
ATCTGGTTCCTGACTTGCAA+AGG 0.535675 4:-26077555 MsaT019281.1:CDS
GATAGATGAGAAAGCATCAC+TGG 0.538809 4:+26077585 None:intergenic
TAAAGCTTCCAGGCTAAAGC+GGG 0.539068 4:+26082709 None:intergenic
CTATAGCTCGATGAATAAGA+TGG 0.539156 4:+26081165 None:intergenic
AACCCTTATGTGTACCCTTG+GGG 0.539200 4:-26071304 MsaT019281.1:CDS
GAAAGGAAAACAGTTGCAGC+TGG 0.543442 4:-26070521 MsaT019281.1:CDS
TGAACAACATGCACAATCCC+AGG 0.543969 4:+26082508 None:intergenic
CCAAATCACGCGATCCGTCT+CGG 0.544998 4:-26082681 MsaT019281.1:CDS
GCAATTTCTTCTCAGAACAC+TGG 0.545545 4:-26080831 MsaT019281.1:CDS
CTGCAACAACAATGTCAAGA+TGG 0.545814 4:-26078261 MsaT019281.1:intron
AAATAGAATTTGCAACCCCA+AGG 0.546112 4:+26071289 None:intergenic
TTTACCCACTGTGGTTGAAG+GGG 0.546589 4:+26070067 None:intergenic
TTACCCCAACAAATCCAAAA+GGG 0.549849 4:+26069971 None:intergenic
AGAATTCTGGACAGAATTGG+TGG 0.554152 4:+26070721 None:intergenic
GGATTGTGCATGTTGTTCAA+GGG 0.554489 4:-26082504 MsaT019281.1:CDS
GAATGGCATTAAACAAGATA+GGG 0.554755 4:-26070388 MsaT019281.1:CDS
TGCAGTTAACGGCCTTCAGG+TGG 0.554887 4:-26070831 MsaT019281.1:CDS
CGGAACCGATCTGGTGGAGA+AGG 0.557316 4:+26082737 None:intergenic
AGGGTACACATAAGGGTTGG+AGG 0.558649 4:+26071309 None:intergenic
GACCGGCGGAACCGATCTGG+TGG 0.559500 4:+26082731 None:intergenic
TGCTTTCTCATCTATCCCAT+CGG 0.560584 4:-26077578 MsaT019281.1:CDS
GGTATCAAGTTCTTTGGCAA+GGG 0.560592 4:+26073616 None:intergenic
GTTGGAATTTCCAGATGCAC+CGG 0.560597 4:+26070424 None:intergenic
TTTGCTTTGCCTAGGAGACA+TGG 0.560638 4:-26071406 MsaT019281.1:CDS
GGAGCACCACTTCTTCAGCT+GGG 0.562071 4:-26078352 MsaT019281.1:CDS
TAATTACACTGCTCCTGAGA+TGG 0.566358 4:-26078378 MsaT019281.1:CDS
CTTGCGAGCAATTAAATGGT+AGG 0.568377 4:+26078295 None:intergenic
GTCAATGCTTTGCTTTGCCT+AGG 0.569127 4:-26071414 MsaT019281.1:CDS
CGCCGAGCTAATAATAAACA+AGG 0.570376 4:-26073843 MsaT019281.1:intron
GTAAAGCTTCCAGGCTAAAG+CGG 0.572878 4:+26082708 None:intergenic
GCATGGTTTACTTCAGATTG+CGG 0.574438 4:-26081216 MsaT019281.1:CDS
TTACCCACTGTGGTTGAAGG+GGG 0.576336 4:+26070068 None:intergenic
AAGTCAGGAACCAGATCCGA+TGG 0.578931 4:+26077562 None:intergenic
GGATTGTTGGAGGTGAAGCA+TGG 0.582250 4:-26081233 MsaT019281.1:CDS
ATAGCACCCAGCTGAAGAAG+TGG 0.584812 4:+26078346 None:intergenic
CTAAGTTCTGCACAAAGTGG+TGG 0.585328 4:+26074647 None:intergenic
CCAGGCTAAAGCGGGACCGG+CGG 0.586173 4:+26082717 None:intergenic
TCGGAGGCGAGATTGAAGGC+TGG 0.591058 4:-26082607 MsaT019281.1:CDS
TCAAAATTCTTACAAGCATG+TGG 0.591284 4:+26075663 None:intergenic
GCACCGGATAATGAACCACT+AGG 0.592652 4:+26070440 None:intergenic
TGCCAAAGAACTTGATACCC+AGG 0.592784 4:-26073612 MsaT019281.1:intron
GATGAAACAACATTTGATGC+CGG 0.594371 4:+26070176 None:intergenic
GGACAGCATGTTTATCAAGT+CGG 0.595468 4:+26071379 None:intergenic
GCCTTCAGGTGGAAGCTCCA+AGG 0.597591 4:-26070820 MsaT019281.1:CDS
CAGTGCTTAAGACAGGGACA+AGG 0.599466 4:+26073900 None:intergenic
TTCAGTATCACCAAATTGTG+AGG 0.603278 4:+26070541 None:intergenic
CCAAGGGTACACATAAGGGT+TGG 0.603498 4:+26071306 None:intergenic
CAACCACAGTGGGTAAAGGG+CGG 0.605534 4:-26070061 MsaT019281.1:CDS
GAGTAAGAATGCGATGGCGA+TGG 0.606867 4:-26082473 MsaT019281.1:CDS
TGGTTAGGTTGAGACATCCT+GGG 0.607429 4:-26082525 MsaT019281.1:CDS
TTGTTGTTGCAGTCAAACAA+AGG 0.610321 4:+26078272 None:intergenic
GATAATGAACCACTAGGGCG+TGG 0.610882 4:+26070446 None:intergenic
CTCGATGAATAAGATGGGCA+TGG 0.611719 4:+26081171 None:intergenic
GATTGGCCTCCACGCCCTAG+TGG 0.614962 4:-26070455 MsaT019281.1:CDS
GGATACTGATGCTGCCGAGA+CGG 0.614983 4:+26082667 None:intergenic
TAATTAATGGATGGCTGAAG+TGG 0.616939 4:+26078395 None:intergenic
AATAGAATTTGCAACCCCAA+GGG 0.617052 4:+26071290 None:intergenic
TGCCAAGTCCCAAAATTCTG+TGG 0.619306 4:-26070306 MsaT019281.1:CDS
GAATTCTGGACAGAATTGGT+GGG 0.619332 4:+26070722 None:intergenic
AGTGGAGCCTGGAAACTTGG+TGG 0.619812 4:-26080864 MsaT019281.1:CDS
TACGACCTTCTCCACCAGAT+CGG 0.620628 4:-26082742 MsaT019281.1:CDS
TCCCCGCCCTTTACCCACTG+TGG 0.621264 4:+26070058 None:intergenic
AGCTGTTGAGCAGTCAGGAG+TGG 0.622583 4:+26071103 None:intergenic
GGAGCAGTGTAATTAATGGA+TGG 0.623150 4:+26078386 None:intergenic
TATAGCTCGATGAATAAGAT+GGG 0.623474 4:+26081166 None:intergenic
ATACCTTGTATCGTAATACA+CGG 0.624670 4:+26075489 None:intergenic
AGATTGGGGTCCTAAAGCAA+CGG 0.625317 4:-26070747 MsaT019281.1:CDS
CCCAACAAATCCAAAAGGGG+TGG 0.627609 4:+26069975 None:intergenic
GTCCTTCAGTAGATATAGAG+TGG 0.630972 4:-26070589 MsaT019281.1:CDS
GTTCTGCACAAAGTGGTGGA+AGG 0.631669 4:+26074651 None:intergenic
CAGAAGCAGTGCTTAAGACA+GGG 0.633050 4:+26073894 None:intergenic
GCATCTGGAAATTCCAACAA+TGG 0.635190 4:-26070419 MsaT019281.1:CDS
AATTCTGGACAGAATTGGTG+GGG 0.638013 4:+26070723 None:intergenic
ACACTGCTCCTGAGATGGTG+AGG 0.638853 4:-26078373 MsaT019281.1:CDS
TCATCGAGCTATAGCACCCG+AGG 0.639167 4:-26081156 MsaT019281.1:intron
GGATGAGAGTAAGAATGCGA+TGG 0.639503 4:-26082479 MsaT019281.1:CDS
AGAAGGTCGTAGTCTTGAAG+AGG 0.641764 4:+26082754 None:intergenic
GGCCACTCTATATCTACTGA+AGG 0.646021 4:+26070587 None:intergenic
TACCTTGTATCGTAATACAC+GGG 0.650881 4:+26075490 None:intergenic
ATTTGATTAGGATGGATGCT+GGG 0.651192 4:-26082552 MsaT019281.1:CDS
TTCCTAAGTTCTGCACAAAG+TGG 0.656952 4:+26074644 None:intergenic
TTCGCAACGGAAGCAAACAA+CGG 0.657834 4:+26082442 None:intergenic
ACATAAGGGTTGGAGGAGAG+CGG 0.662635 4:+26071316 None:intergenic
AATGAACCACTAGGGCGTGG+AGG 0.667047 4:+26070449 None:intergenic
AGTGCTTAAGACAGGGACAA+GGG 0.667307 4:+26073901 None:intergenic
TACCCCAACAAATCCAAAAG+GGG 0.667620 4:+26069972 None:intergenic
ATCTGTGATAGGAAACCCTG+TGG 0.670211 4:-26070681 MsaT019281.1:CDS
AGTCAGGAACCAGATCCGAT+GGG 0.671346 4:+26077563 None:intergenic
TAGTACCTGAGACTCTGCAA+TGG 0.674895 4:+26074583 None:intergenic
ATTGAAGGCTGGATTGACCA+AGG 0.675287 4:-26082596 MsaT019281.1:CDS
TCTATCAGTAACCTGAACAA+TGG 0.676581 4:-26070248 MsaT019281.1:CDS
ACTGAAAACAACAGTTGCTG+CGG 0.682367 4:-26072552 MsaT019281.1:intron
CACCGGATAATGAACCACTA+GGG 0.684290 4:+26070441 None:intergenic
CTTCCCAAGAGTATTCGCAA+CGG 0.694318 4:+26082429 None:intergenic
TGGATGTTACTTGACCCACA+GGG 0.701888 4:+26070666 None:intergenic
AGACCAGAAGATGCACGAGG+CGG 0.718681 4:+26070566 None:intergenic
GACTGATTCTGGAATCCCAG+AGG 0.719882 4:-26070867 MsaT019281.1:CDS
TCTGTGATAGGAAACCCTGT+GGG 0.720294 4:-26070680 MsaT019281.1:CDS
AGTAAGATAGAAGAGAAGCG+AGG 0.733585 4:-26070899 MsaT019281.1:CDS
AGTGCTAGTCCATGAACACG+TGG 0.738043 4:+26072572 None:intergenic
AAGAACCCAAGAGAACTCTG+AGG 0.741155 4:+26070218 None:intergenic
GTAAGACCAGAAGATGCACG+AGG 0.776735 4:+26070563 None:intergenic
GAAGGCTGGATTGACCAAGG+CGG 0.788673 4:-26082593 MsaT019281.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 26069971 26082878 26069971 ID=MsaG019281
Chr4 mRNA 26069971 26082878 26069971 ID=MsaT019281.1;Parent=MsaG019281
Chr4 exon 26069971 26070919 26069971 ID=MsaT019281.1.exon14;Parent=MsaT019281.1
Chr4 CDS 26069971 26070919 26069971 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26071052 26071170 26071052 ID=MsaT019281.1.exon13;Parent=MsaT019281.1
Chr4 CDS 26071052 26071170 26071052 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26071281 26071442 26071281 ID=MsaT019281.1.exon12;Parent=MsaT019281.1
Chr4 CDS 26071281 26071442 26071281 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26072553 26072615 26072553 ID=MsaT019281.1.exon11;Parent=MsaT019281.1
Chr4 CDS 26072553 26072615 26072553 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26073613 26073741 26073613 ID=MsaT019281.1.exon10;Parent=MsaT019281.1
Chr4 CDS 26073613 26073741 26073613 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26073844 26073957 26073844 ID=MsaT019281.1.exon9;Parent=MsaT019281.1
Chr4 CDS 26073844 26073957 26073844 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26074589 26074675 26074589 ID=MsaT019281.1.exon8;Parent=MsaT019281.1
Chr4 CDS 26074589 26074675 26074589 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26075493 26075579 26075493 ID=MsaT019281.1.exon7;Parent=MsaT019281.1
Chr4 CDS 26075493 26075579 26075493 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26075676 26075737 26075676 ID=MsaT019281.1.exon6;Parent=MsaT019281.1
Chr4 CDS 26075676 26075737 26075676 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26077506 26077632 26077506 ID=MsaT019281.1.exon5;Parent=MsaT019281.1
Chr4 CDS 26077506 26077632 26077506 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26078262 26078444 26078262 ID=MsaT019281.1.exon4;Parent=MsaT019281.1
Chr4 CDS 26078262 26078444 26078262 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26080797 26080904 26080797 ID=MsaT019281.1.exon3;Parent=MsaT019281.1
Chr4 CDS 26080797 26080904 26080797 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26081157 26081261 26081157 ID=MsaT019281.1.exon2;Parent=MsaT019281.1
Chr4 CDS 26081157 26081261 26081157 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Chr4 exon 26082387 26082878 26082387 ID=MsaT019281.1.exon1;Parent=MsaT019281.1
Chr4 CDS 26082387 26082878 26082387 ID=cds.MsaT019281.1;Parent=MsaT019281.1
Gene Sequence

>MsaT019281.1

ATGTCCCTAAACATGAAAACCCTAACCCAAGCCTTCGCAAAAACCGCAACCCTAATAGAAAAAACCGTTTCAACAACCGTTCAAGAAGTAACAGGTCCAAAGCCTCTTCAAGACTACGACCTTCTCCACCAGATCGGTTCCGCCGGTCCCGCTTTAGCCTGGAAGCTTTACTCCGCCAAATCACGCGATCCGTCTCGGCAGCATCAGTATCCGGTTGTTTGCGTTTGGGTGTTGGATAAAAAGGCGTTGTCGGAGGCGAGATTGAAGGCTGGATTGACCAAGGCGGCTGAGGATGCTTTTTTGGATTTGATTAGGATGGATGCTGGGAAGATGGTTAGGTTGAGACATCCTGGGATTGTGCATGTTGTTCAAGGGTTGGATGAGAGTAAGAATGCGATGGCGATGGTTACTGAACCGTTGTTTGCTTCCGTTGCGAATACTCTTGGGAAGCTTGATAATGTTCAAAGTGTTCCTAAGGATCTTAAGGGAATGGAAATGGGATTGTTGGAGGTGAAGCATGGTTTACTTCAGATTGCGGAGTCGTTGGATTTTCTCCACAACCATGCCCATCTTATTCATCGAGCTATAGCACCCGAGAATGTTTTTATCACATTGAGTGGAGCCTGGAAACTTGGTGGATTTGGTTTTGCAATTTCTTCTCAGAACACTGGTGACTCATCTAATCTGCATGCTTTCCATTATGCTGAATATGATGTTGAAGACTCTGTTTTGCCACTTCAGCCATCCATTAATTACACTGCTCCTGAGATGGTGAGGAGCACCACTTCTTCAGCTGGGTGCTATTCTGATATTTTCAGTTTTGGATGTCTTGCCTACCATTTAATTGCTCGCAAGCCTTTGTTTGACTGCAACAACAATGTCAAGATGTACATGAATACATTGACTTACTTATCCAGTGATGCTTTCTCATCTATCCCATCGGATCTGGTTCCTGACTTGCAAAGGATGCTCTCTTCAAATGAATCTTTCAGGCCAAGTGCAATGGATTTTACAGGCTCACCATTTTTTCGGAATGACACTAGGTTACGTGCTCTGCGTTTCCTGGACCACATGCTTGAAAGAGATAACATGCAGAAGTCAGAGTTCTTAAAAGCATTGTCAGATATGTGGAAAGATTTTGATTCCCGTGTATTACGATACAAGGTCCTTCCACCACTTTGTGCAGAACTTAGGAATGTCGTTATACAACCGATGATTCTACCAATGGTTTTAACCATTGCAGAGTCTCAGGACAAGAATGACTTTGAGCAATCAACACTTCCAGCCCTTGTCCCTGTCTTAAGCACTGCTTCTGGTGATACAATGCTACTGCTTCTGAAGCACGCCGAGCTAATAATAAACAAGACTAGTCAAGACCATTTAATTTCACATGTTCTTCCAATGATTGTTCGAGCCTATGATGATAATGATTCACGTTTACAAGAAGAGGTGTTGAAAAAGTCGGTATCCCTTGCCAAAGAACTTGATACCCAGCTGGTGAAACAAGTGATTTTACCACGTGTTCATGGACTAGCACTGAAAACAACAGTTGCTGCGGTTAGAGTCAATGCTTTGCTTTGCCTAGGAGACATGGTCAACCGACTTGATAAACATGCTGTCCTGGAAATCTTGCAAACTATTCAGCGTTGCACTGCTGTTGACCGCTCTCCTCCAACCCTTATGTGTACCCTTGGGGTTGCAAATTCTATTTTTAAGCAGTATGGAGTAGAATTTGTTGCTGAGCATGTTCTTCCATTGCTTATGCCACTCCTGACTGCTCAACAGCTGAATGTTCAACAGTTTGCCAAATATATGCTCTTTGTCAAGAATATCCTCCATAAGATAGAAGAGAAGCGAGGAGTTGCTGTGACTGATTCTGGAATCCCAGAGGTAAAACTATCTCCTGCAGTTAACGGCCTTCAGGTGGAAGCTCCAAGGACAACCAGCAGCACCGTTGCTTCAACAAAAAGCAGCTTCTCTTGGGATGCAGATTGGGGTCCTAAAGCAACGGGCCCCACCAATTCTGTCCAGAATTCTATTAATACATCTAATAAATCTGTGATAGGAAACCCTGTGGGTCAAGTAACATCCATACAAAATAATTTGCCCTTATCTGGTGTATCTAATCAGCAAACATCCAATTCATGTCCTTCAGTAGATATAGAGTGGCCGCCTCGTGCATCTTCTGGTCTTACCTCACAATTTGGTGATACTGAAAGGAAAACAGTTGCAGCTGGAACCTCATCCACATCCATTATTGAAGATGACGATCCTTTTGCTGATTGGCCTCCACGCCCTAGTGGTTCATTATCCGGTGCATCTGGAAATTCCAACAATGGTATTTCAGGAATGGCATTAAACAAGATAGGGCATAATTCAATGACAAGCAATTTAAGCAATCTTGGTCTCCAGGCCAGCAACAATTGGTCTGCCAAGTCCCAAAATTCTGTGGAGTCTATTGGTTTGAACCCAAGGAATGCTAGCTCATCTATCAGTAACCTGAACAATGGCTTTGAACCTCAGAGTTCTCTTGGGTTCTTGAAACAAAGTCAGGCTTTTCCGGCATCAAATGTTGTTTCATCATCATATAATAATGTTAAATCAACTGATCTTGGATCAATATTTTCTTCCAACAAGAATGAACAACTTGCACCTAGACTCGCACCACCCCCTTCAACCACAGTGGGTAAAGGGCGGGGAAGAGGCAGAGGAGCAACTTCATCCAGACAGCACTCTCATACCAAGTCAAGCACTGAGCAGCCACCCCTTTTGGATTTGTTGGGGTAA
Protein sequence

>MsaT019281.1

MSLNMKTLTQAFAKTATLIEKTVSTTVQEVTGPKPLQDYDLLHQIGSAGPALAWKLYSAKSRDPSRQHQYPVVCVWVLDKKALSEARLKAGLTKAAEDAFLDLIRMDAGKMVRLRHPGIVHVVQGLDESKNAMAMVTEPLFASVANTLGKLDNVQSVPKDLKGMEMGLLEVKHGLLQIAESLDFLHNHAHLIHRAIAPENVFITLSGAWKLGGFGFAISSQNTGDSSNLHAFHYAEYDVEDSVLPLQPSINYTAPEMVRSTTSSAGCYSDIFSFGCLAYHLIARKPLFDCNNNVKMYMNTLTYLSSDAFSSIPSDLVPDLQRMLSSNESFRPSAMDFTGSPFFRNDTRLRALRFLDHMLERDNMQKSEFLKALSDMWKDFDSRVLRYKVLPPLCAELRNVVIQPMILPMVLTIAESQDKNDFEQSTLPALVPVLSTASGDTMLLLLKHAELIINKTSQDHLISHVLPMIVRAYDDNDSRLQEEVLKKSVSLAKELDTQLVKQVILPRVHGLALKTTVAAVRVNALLCLGDMVNRLDKHAVLEILQTIQRCTAVDRSPPTLMCTLGVANSIFKQYGVEFVAEHVLPLLMPLLTAQQLNVQQFAKYMLFVKNILHKIEEKRGVAVTDSGIPEVKLSPAVNGLQVEAPRTTSSTVASTKSSFSWDADWGPKATGPTNSVQNSINTSNKSVIGNPVGQVTSIQNNLPLSGVSNQQTSNSCPSVDIEWPPRASSGLTSQFGDTERKTVAAGTSSTSIIEDDDPFADWPPRPSGSLSGASGNSNNGISGMALNKIGHNSMTSNLSNLGLQASNNWSAKSQNSVESIGLNPRNASSSISNLNNGFEPQSSLGFLKQSQAFPASNVVSSSYNNVKSTDLGSIFSSNKNEQLAPRLAPPPSTTVGKGRGRGRGATSSRQHSHTKSSTEQPPLLDLLG*