AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019313

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019313 MtrunA17_Chr4g0014031 72.650 234 47 2 1 222 1 229 1.66e-114 326
MsaG019313 MtrunA17_Chr4g0014131 68.852 244 41 2 1 222 1 231 2.66e-109 313
MsaG019313 MtrunA17_Chr4g0014321 58.009 231 88 1 1 222 1 231 3.34e-94 275
MsaG019313 MtrunA17_Chr8g0351751 57.085 247 81 4 1 222 1 247 2.64e-89 263
MsaG019313 MtrunA17_Chr4g0013041 60.513 195 69 2 1 195 1 187 2.89e-83 246
MsaG019313 MtrunA17_Chr4g0014121 80.255 157 31 0 1 157 29 185 8.67e-82 242
MsaG019313 MtrunA17_Chr3g0110131 49.378 241 97 4 1 219 1 238 5.13e-66 204
MsaG019313 MtrunA17_Chr3g0107381 46.121 232 100 5 1 218 1 221 5.55e-65 200
MsaG019313 MtrunA17_Chr2g0296831 72.131 122 34 0 1 122 1 122 5.86e-51 161
MsaG019313 MtrunA17_Chr5g0432691 47.742 155 80 1 1 155 1 154 1.55e-46 151
MsaG019313 MtrunA17_Chr3g0107331 49.007 151 76 1 3 153 2 151 4.05e-46 150
MsaG019313 MtrunA17_Chr3g0107461 43.590 156 88 0 1 156 1 156 1.04e-43 144
MsaG019313 MtrunA17_Chr3g0107351 46.711 152 80 1 3 154 2 152 1.42e-43 144
MsaG019313 MtrunA17_Chr5g0420351 46.154 156 82 2 1 156 1 154 1.62e-41 138
MsaG019313 MtrunA17_Chr3g0107341 46.053 152 81 1 3 154 2 152 4.24e-41 137
MsaG019313 MtrunA17_Chr3g0107361 44.737 152 83 1 3 154 2 152 4.68e-41 137
MsaG019313 MtrunA17_Chr2g0283941 39.560 182 108 2 1 180 1 182 7.16e-41 139
MsaG019313 MtrunA17_Chr1g0188681 42.308 156 88 2 1 156 1 154 1.86e-37 128
MsaG019313 MtrunA17_Chr1g0197461 42.208 154 87 2 3 156 2 153 7.20e-36 124
MsaG019313 MtrunA17_Chr3g0105791 44.444 126 70 0 29 154 1 126 1.48e-35 122
MsaG019313 MtrunA17_Chr1g0188661 44.231 156 85 2 1 156 1 154 2.24e-35 122
MsaG019313 MtrunA17_Chr7g0218151 43.590 156 86 2 1 156 1 154 6.65e-35 121
MsaG019313 MtrunA17_Chr4g0012761 44.231 156 85 2 1 156 1 154 1.00e-34 121
MsaG019313 MtrunA17_Chr1g0193311 44.872 156 84 2 1 156 1 154 4.69e-34 119
MsaG019313 MtrunA17_Chr4g0031421 44.444 126 70 0 29 154 1 126 1.00e-33 118
MsaG019313 MtrunA17_Chr1g0197321 38.961 154 93 1 1 154 1 153 1.29e-33 118
MsaG019313 MtrunA17_Chr1g0197261 40.667 150 87 2 1 150 1 148 4.51e-33 117
MsaG019313 MtrunA17_Chr1g0188651 43.590 156 84 2 1 156 1 152 6.54e-33 116
MsaG019313 MtrunA17_Chr3g0106421 32.653 196 120 4 29 219 1 189 3.18e-30 111
MsaG019313 MtrunA17_Chr2g0296861 38.217 157 96 1 1 157 1 156 9.55e-27 100
MsaG019313 MtrunA17_Chr7g0267601 39.370 127 77 0 3 129 6 132 8.35e-25 101
MsaG019313 MtrunA17_Chr4g0008701 40.157 127 76 0 3 129 6 132 1.63e-24 100
MsaG019313 MtrunA17_Chr3g0128721 31.690 142 97 0 3 144 6 147 1.27e-21 92.4
MsaG019313 MtrunA17_Chr3g0106501 33.594 128 85 0 29 156 1 128 4.00e-20 83.2
MsaG019313 MtrunA17_Chr2g0296851 58.025 81 34 0 74 154 1 81 4.27e-19 79.0
MsaG019313 MtrunA17_Chr1g0212151 31.858 113 70 3 1 107 1 112 1.81e-13 68.6
MsaG019313 MtrunA17_Chr6g0457851 32.743 113 69 4 1 107 11 122 3.57e-13 67.8
MsaG019313 MtrunA17_Chr6g0449861 33.628 113 68 4 1 107 9 120 1.96e-12 65.5
MsaG019313 MtrunA17_Chr6g0449851 35.398 113 66 3 1 107 1 112 2.29e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019313 AT5G48670.1 55.195 154 68 1 1 154 1 153 9.57e-46 154
MsaG019313 AT1G65330.1 42.246 187 94 3 3 176 2 187 4.76e-41 141
MsaG019313 AT1G65300.1 41.436 181 97 2 3 175 2 181 8.29e-41 140
MsaG019313 AT5G26630.1 44.503 191 95 2 1 181 1 190 1.02e-39 136
MsaG019313 AT5G26650.1 36.667 210 114 4 5 204 3 203 8.59e-36 130
MsaG019313 AT5G27960.1 40.476 168 89 3 5 162 3 169 3.46e-35 127
MsaG019313 AT1G31630.1 36.905 168 95 3 3 161 2 167 3.74e-34 125
MsaG019313 AT1G31640.1 39.735 151 89 2 3 153 2 150 2.51e-32 122
MsaG019313 AT3G05860.2 37.975 158 97 1 1 158 1 157 4.91e-31 113
MsaG019313 AT3G05860.3 37.975 158 97 1 1 158 1 157 7.46e-31 114
MsaG019313 AT3G05860.1 37.975 158 97 1 1 158 1 157 9.07e-31 114
MsaG019313 AT5G27810.1 55.914 93 41 0 29 121 1 93 2.17e-30 109
MsaG019313 AT2G28700.1 38.037 163 96 2 1 161 1 160 2.35e-29 112
MsaG019313 AT1G22590.2 32.099 162 106 1 1 162 1 158 1.40e-26 100
MsaG019313 AT5G26580.1 33.333 180 97 4 1 172 1 165 6.48e-24 97.8
MsaG019313 AT5G06500.1 35.669 157 99 2 1 157 1 155 7.30e-23 93.2
MsaG019313 AT2G40210.1 37.984 129 79 1 1 129 1 128 1.06e-17 80.9
MsaG019313 AT5G58890.1 37.795 127 68 5 6 123 6 130 2.81e-15 73.6
MsaG019313 AT5G55690.1 42.308 78 38 2 1 71 1 78 2.33e-12 65.1
MsaG019313 AT5G55690.2 42.308 78 38 2 1 71 1 78 2.33e-12 65.1
MsaG019313 AT5G27090.1 34.444 90 58 1 7 95 23 112 7.68e-11 59.7
MsaG019313 AT5G27944.1 39.726 73 43 1 24 95 41 113 9.57e-11 60.1

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAACAAAGAAAAGAAAAT+AGG 0.231520 4:+26792137 MsaT019313.1:CDS
CCCTTTGGTGAGATCAATTA+TGG 0.234667 4:+26792454 MsaT019313.1:CDS
TACCAATGTCCTTCAAATTA+TGG 0.254451 4:-26792264 None:intergenic
ATAATTTCATCATCATTAAT+AGG 0.285530 4:-26792376 None:intergenic
GTGAGATCAATTATGGAGTT+TGG 0.309548 4:+26792461 MsaT019313.1:CDS
AACAAAGGGTCTATTGAAAA+AGG 0.314581 4:+26791904 MsaT019313.1:CDS
AGCATCTAGGAGTGAGGAAA+TGG 0.344298 4:+26792324 MsaT019313.1:CDS
ATGAGCCTCAACCTGAGATT+TGG 0.345571 4:+26791984 MsaT019313.1:CDS
ATCCTTCAAGGTAGTAAATC+AGG 0.372648 4:-26792484 None:intergenic
TATGCAAAGTCAATTATTGA+TGG 0.385590 4:+26792396 MsaT019313.1:CDS
CTGATTTGAGTTATCTTACT+TGG 0.390292 4:+26792233 MsaT019313.1:CDS
GAAGGACATTGGTAGACGTT+TGG 0.410243 4:+26792273 MsaT019313.1:CDS
CTAGATGCTGTTGGAGGTGG+TGG 0.417295 4:-26792310 None:intergenic
GAAACAAAGAAAAGAAAATA+GGG 0.419680 4:+26792138 MsaT019313.1:CDS
GGCCTGATTTACTACCTTGA+AGG 0.422090 4:+26792482 MsaT019313.1:CDS
AGTGAGGAAATGGGAATTGT+GGG 0.443118 4:+26792334 MsaT019313.1:CDS
GTTGATTGACCATAATTTGA+AGG 0.445081 4:+26792255 MsaT019313.1:CDS
TTGTGGGTCATGGCCATGTT+GGG 0.452922 4:+26792350 MsaT019313.1:CDS
CACAACCATGGCTAGAAGTA+AGG 0.457305 4:+26791826 None:intergenic
TCTGGTGAAGGCCAAATCTC+AGG 0.467267 4:-26791995 None:intergenic
CTCATAAGAGCCATACACTA+TGG 0.467809 4:-26791966 None:intergenic
ATTGTGGGTCATGGCCATGT+TGG 0.468248 4:+26792349 MsaT019313.1:CDS
CTCCTAGATGCTGTTGGAGG+TGG 0.476689 4:-26792313 None:intergenic
TCCTCACTCCTAGATGCTGT+TGG 0.486555 4:-26792319 None:intergenic
CTTGACACAAAGGATTCTGA+AGG 0.491635 4:+26792099 MsaT019313.1:CDS
TGCAGCATCATTGACAATGA+AGG 0.502295 4:-26791858 None:intergenic
TTTGTGTCAAGAAAGTCTCT+TGG 0.512375 4:-26792087 None:intergenic
GATGCTGTTGGAGGTGGTGG+TGG 0.513228 4:-26792307 None:intergenic
GAGTGAGGAAATGGGAATTG+TGG 0.516317 4:+26792333 MsaT019313.1:CDS
GACCATAATTTGAAGGACAT+TGG 0.517963 4:+26792262 MsaT019313.1:CDS
GGACATTGGTAGACGTTTGG+CGG 0.524285 4:+26792276 MsaT019313.1:CDS
TCACTCCTAGATGCTGTTGG+AGG 0.527908 4:-26792316 None:intergenic
CACCACCTCCAACAGCATCT+AGG 0.540352 4:+26792311 MsaT019313.1:CDS
ATGTCTCATTGTCTCAATGA+AGG 0.542205 4:+26792181 MsaT019313.1:CDS
GCATCTAGGAGTGAGGAAAT+GGG 0.544227 4:+26792325 MsaT019313.1:CDS
ATTATTGATGGACTCAATGA+TGG 0.556842 4:+26792408 MsaT019313.1:CDS
CCATAATTGATCTCACCAAA+GGG 0.558369 4:-26792454 None:intergenic
GAAGCCTGTGCCATAGTGTA+TGG 0.560968 4:+26791956 MsaT019313.1:CDS
GAGATTTGGCCTTCACCAGA+GGG 0.565461 4:+26791998 MsaT019313.1:CDS
TTTAACCTTACTTCTAGCCA+TGG 0.572129 4:-26791831 None:intergenic
GATTTGGCCTTCACCAGAGG+GGG 0.573799 4:+26792000 MsaT019313.1:CDS
TCCATAATTGATCTCACCAA+AGG 0.580970 4:-26792455 None:intergenic
GTGCACGAGACAGTGCCCTT+TGG 0.582311 4:+26792439 MsaT019313.1:CDS
TTGATGGACTCAATGATGGC+TGG 0.587358 4:+26792412 MsaT019313.1:CDS
AAGAGACTTTCTTGACACAA+AGG 0.587821 4:+26792089 MsaT019313.1:CDS
CATTAATAGGTATCCCAACA+TGG 0.589301 4:-26792363 None:intergenic
GAAGGCCAAATCTCAGGTTG+AGG 0.598100 4:-26791989 None:intergenic
ACAGGCTTCAATGCCACAGA+GGG 0.601811 4:-26791942 None:intergenic
TGAGATTTGGCCTTCACCAG+AGG 0.614269 4:+26791997 MsaT019313.1:CDS
GAAATGGGAATTGTGGGTCA+TGG 0.616732 4:+26792340 MsaT019313.1:CDS
TCCAACAGCATCTAGGAGTG+AGG 0.643310 4:+26792318 MsaT019313.1:CDS
AGATTTGGCCTTCACCAGAG+GGG 0.650878 4:+26791999 MsaT019313.1:CDS
TCATTGTCAATGATGCTGCA+AGG 0.652548 4:+26791861 MsaT019313.1:CDS
AGAGCCATACACTATGGCAC+AGG 0.656790 4:-26791960 None:intergenic
TGTGCACGACAATATGTCAA+CGG 0.676436 4:+26792210 MsaT019313.1:CDS
GCTGAACTTAGCACCCTCTG+TGG 0.677042 4:+26791929 MsaT019313.1:CDS
CACAGGCTTCAATGCCACAG+AGG 0.696692 4:-26791943 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 26791833 26792501 26791833 ID=MsaG019313
Chr4 mRNA 26791833 26792501 26791833 ID=MsaT019313.1;Parent=MsaG019313
Chr4 exon 26791833 26792501 26791833 ID=MsaT019313.1.exon1;Parent=MsaT019313.1
Chr4 CDS 26791833 26792501 26791833 ID=cds.MsaT019313.1;Parent=MsaT019313.1
Gene Sequence

>MsaT019313.1

ATGGCTAGAAGTAAGGTTAAACTCGCCTTCATTGTCAATGATGCTGCAAGGAAAGCAACATACAAAAAAAGAACAAAGGGTCTATTGAAAAAGGTTGCTGAACTTAGCACCCTCTGTGGCATTGAAGCCTGTGCCATAGTGTATGGCTCTTATGAGCCTCAACCTGAGATTTGGCCTTCACCAGAGGGGGTCCAATCTGTGCTCTCAAAATTCATGACGATGCACGAGTTTCAAAAGTGCAAGAAAATGCTGAACCAAGAGACTTTCTTGACACAAAGGATTCTGAAGGCTGAAGAGAAATTGAAGAAACAAAGAAAAGAAAATAGGGAGCAAGAGATGACAATACTCATGTCTCATTGTCTCAATGAAGGTAGAGTTGTGCACGACAATATGTCAACGGCTGATTTGAGTTATCTTACTTGGTTGATTGACCATAATTTGAAGGACATTGGTAGACGTTTGGCGGCAACAGTGCCACCACCACCTCCAACAGCATCTAGGAGTGAGGAAATGGGAATTGTGGGTCATGGCCATGTTGGGATACCTATTAATGATGATGAAATTATGCAAAGTCAATTATTGATGGACTCAATGATGGCTGGTAATGTGCACGAGACAGTGCCCTTTGGTGAGATCAATTATGGAGTTTGGCCTGATTTACTACCTTGA
Protein sequence

>MsaT019313.1

MARSKVKLAFIVNDAARKATYKKRTKGLLKKVAELSTLCGIEACAIVYGSYEPQPEIWPSPEGVQSVLSKFMTMHEFQKCKKMLNQETFLTQRILKAEEKLKKQRKENREQEMTILMSHCLNEGRVVHDNMSTADLSYLTWLIDHNLKDIGRRLAATVPPPPPTASRSEEMGIVGHGHVGIPINDDEIMQSQLLMDSMMAGNVHETVPFGEINYGVWPDLLP*