AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019318


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019318 MtrunA17_Chr4g0014321 65.801 231 42 2 1 194 1 231 1.23e-102 295
MsaG019318 MtrunA17_Chr8g0351751 48.954 239 74 3 1 191 1 239 1.05e-70 214
MsaG019318 MtrunA17_Chr4g0013041 54.455 202 42 3 1 167 1 187 4.36e-67 204
MsaG019318 MtrunA17_Chr4g0014031 45.299 234 83 4 1 194 1 229 2.66e-53 170
MsaG019318 MtrunA17_Chr4g0014131 41.803 244 79 5 1 194 1 231 1.18e-44 147
MsaG019318 MtrunA17_Chr3g0110131 37.553 237 91 4 1 186 1 231 5.88e-42 141
MsaG019318 MtrunA17_Chr4g0014121 48.718 156 45 1 1 121 29 184 7.42e-37 126
MsaG019318 MtrunA17_Chr3g0107381 38.750 160 61 2 1 123 1 160 1.48e-35 124
MsaG019318 MtrunA17_Chr2g0296831 65.591 93 32 0 1 93 1 93 1.79e-34 118
MsaG019318 MtrunA17_Chr3g0107331 52.222 90 43 0 4 93 3 92 7.56e-32 113
MsaG019318 MtrunA17_Chr5g0432691 48.387 93 48 0 1 93 1 93 1.39e-28 104
MsaG019318 MtrunA17_Chr2g0283941 48.077 104 50 1 1 104 1 100 2.34e-28 106
MsaG019318 MtrunA17_Chr3g0107351 47.191 89 47 0 5 93 4 92 6.80e-28 102
MsaG019318 MtrunA17_Chr3g0107341 48.315 89 46 0 5 93 4 92 8.62e-28 102
MsaG019318 MtrunA17_Chr3g0107461 43.478 92 52 0 1 92 1 92 6.03e-27 100
MsaG019318 MtrunA17_Chr3g0107361 46.067 89 48 0 5 93 4 92 7.08e-27 100
MsaG019318 MtrunA17_Chr5g0420351 45.161 93 50 1 1 93 1 92 2.43e-26 98.6
MsaG019318 MtrunA17_Chr1g0188681 45.556 90 48 1 1 90 1 89 3.31e-25 95.9
MsaG019318 MtrunA17_Chr1g0197321 44.444 90 49 1 1 90 1 89 2.11e-24 93.6
MsaG019318 MtrunA17_Chr1g0197261 45.556 90 48 1 1 90 1 89 3.12e-24 93.2
MsaG019318 MtrunA17_Chr1g0197461 47.619 84 43 1 4 87 3 85 4.74e-24 92.8
MsaG019318 MtrunA17_Chr7g0267601 42.222 90 52 0 4 93 7 96 3.58e-21 90.1
MsaG019318 MtrunA17_Chr2g0296861 35.870 92 59 0 1 92 1 92 1.14e-20 84.0
MsaG019318 MtrunA17_Chr4g0008701 40.000 90 54 0 4 93 7 96 1.67e-20 88.2
MsaG019318 MtrunA17_Chr4g0031421 33.858 127 49 1 29 120 1 127 2.80e-20 82.8
MsaG019318 MtrunA17_Chr1g0193311 45.161 93 50 1 1 93 1 92 2.13e-19 80.9
MsaG019318 MtrunA17_Chr3g0105791 48.438 64 33 0 29 92 1 64 5.11e-19 79.3
MsaG019318 MtrunA17_Chr7g0218151 41.935 93 53 1 1 93 1 92 7.64e-19 79.3
MsaG019318 MtrunA17_Chr3g0128721 37.778 90 56 0 4 93 7 96 1.71e-17 79.7
MsaG019318 MtrunA17_Chr1g0188651 45.556 90 48 1 1 90 1 89 5.05e-17 74.3
MsaG019318 MtrunA17_Chr1g0188661 41.111 90 52 1 1 90 1 89 6.58e-17 74.3
MsaG019318 MtrunA17_Chr4g0012761 41.935 93 53 1 1 93 1 92 8.12e-17 73.9
MsaG019318 MtrunA17_Chr3g0106421 31.250 128 53 1 29 121 1 128 1.12e-15 72.4
MsaG019318 MtrunA17_Chr3g0106501 30.645 124 78 3 29 146 1 122 3.66e-15 69.7
MsaG019318 MtrunA17_Chr6g0457851 37.895 95 52 4 5 93 15 108 1.51e-13 67.8
MsaG019318 MtrunA17_Chr1g0212151 35.789 95 54 3 5 93 5 98 1.56e-13 67.8
MsaG019318 MtrunA17_Chr6g0449851 36.842 95 53 3 5 93 5 98 5.27e-12 63.2
MsaG019318 MtrunA17_Chr6g0449861 35.789 95 54 3 5 93 13 106 9.79e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019318 AT5G48670.1 41.250 160 60 1 1 126 1 160 1.31e-30 114
MsaG019318 AT3G05860.2 42.593 108 55 1 1 101 1 108 2.55e-28 105
MsaG019318 AT3G05860.3 42.593 108 55 1 1 101 1 108 6.94e-28 105
MsaG019318 AT3G05860.1 42.593 108 55 1 1 101 1 108 1.10e-27 105
MsaG019318 AT1G65300.1 51.111 90 44 0 4 93 3 92 2.86e-26 102
MsaG019318 AT5G26650.1 50.000 90 45 0 4 93 2 91 2.95e-26 103
MsaG019318 AT5G27960.1 50.000 90 45 0 4 93 2 91 3.41e-26 102
MsaG019318 AT1G65330.1 49.438 89 45 0 5 93 4 92 8.00e-25 98.2
MsaG019318 AT2G28700.1 40.000 90 54 0 1 90 1 90 7.25e-20 85.9
MsaG019318 AT5G27810.1 50.769 65 32 0 29 93 1 65 9.23e-20 80.9
MsaG019318 AT1G31640.1 41.573 89 51 1 5 93 4 91 1.44e-19 85.9
MsaG019318 AT1G31630.1 40.449 89 52 1 5 93 4 91 2.20e-19 84.7
MsaG019318 AT1G22590.2 36.667 90 57 0 1 90 1 90 6.53e-19 80.1
MsaG019318 AT5G06500.1 41.758 91 53 0 1 91 1 91 6.77e-19 81.6
MsaG019318 AT5G26630.1 31.658 199 99 3 1 162 1 199 1.58e-18 80.1
MsaG019318 AT2G40210.1 40.000 95 57 0 1 95 1 95 2.58e-18 82.0
MsaG019318 AT5G26580.1 40.860 93 41 1 1 93 1 79 1.36e-17 79.7
MsaG019318 AT5G58890.1 39.560 91 45 3 1 81 1 91 1.09e-13 68.2
MsaG019318 AT5G55690.1 33.071 127 70 5 1 116 1 123 1.32e-13 67.8
MsaG019318 AT5G55690.2 33.071 127 70 5 1 116 1 123 1.32e-13 67.8

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGCTTGTGCAGTAGTTTA+TGG 0.155587 4:+26870858 MsaT019318.1:CDS
CACAACAATGACTGGGAAAA+AGG 0.190222 4:+26870728 None:intergenic
TCTTCATTACTGAATGTAAA+TGG 0.245246 4:-26871231 None:intergenic
TCATTGTCGATGATGATTCT+AGG 0.260008 4:+26870763 MsaT019318.1:CDS
AGATCTCTGGTTGAGGCTCA+TGG 0.361064 4:-26870884 None:intergenic
TACCAACCTCCTTCAATTTG+TGG 0.372241 4:-26871155 None:intergenic
AATGTTGTCGCCATTATTCA+TGG 0.384588 4:-26871289 None:intergenic
TACATTCAGTAATGAAGAAA+TGG 0.387226 4:+26871236 MsaT019318.1:CDS
TGTGGTGATGGCCAGATCTC+TGG 0.397343 4:-26870897 None:intergenic
TATGCAGAATAATATGTCAT+TGG 0.399656 4:+26871101 MsaT019318.1:intron
GGAAAGCAACATACAATAGA+AGG 0.409123 4:+26870784 MsaT019318.1:CDS
ATGTAAATGGTGGTGGCATT+TGG 0.422156 4:-26871218 None:intergenic
GATCTCTGGTTGAGGCTCAT+GGG 0.428787 4:-26870883 None:intergenic
AGGATGGGTTCTGGCAATGC+TGG 0.431426 4:+26871261 MsaT019318.1:CDS
TCAAAATTCAGAACAATGAC+TGG 0.434871 4:+26870939 MsaT019318.1:CDS
TAATGAAGAAATGGAAAGGA+TGG 0.435935 4:+26871245 MsaT019318.1:CDS
AATTGAAGGAGGTTGGTAGA+AGG 0.442772 4:+26871160 MsaT019318.1:CDS
GGAGATGAGTCGGTGCCCTT+TGG 0.451552 4:+26871351 MsaT019318.1:CDS
GTTGATTGACCACAAATTGA+AGG 0.458305 4:+26871146 MsaT019318.1:CDS
GAAATGGAAAGGATGGGTTC+TGG 0.460660 4:+26871252 MsaT019318.1:CDS
AAGAGACTTACATGAAAGAA+AGG 0.463147 4:+26870991 MsaT019318.1:CDS
GCTGGAATGCCCATGAATAA+TGG 0.463663 4:+26871279 MsaT019318.1:CDS
ATGAGCCTCAACCAGAGATC+TGG 0.467494 4:+26870886 MsaT019318.1:CDS
TTGATTCAAAACATCTTTGT+TGG 0.470119 4:+26871124 MsaT019318.1:CDS
ATCTCTGGTTGAGGCTCATG+GGG 0.492174 4:-26870882 None:intergenic
AGAGATCTGGCCATCACCAC+AGG 0.494291 4:+26870899 MsaT019318.1:CDS
GACCACAAATTGAAGGAGGT+TGG 0.522187 4:+26871153 MsaT019318.1:CDS
ATCACCACAGGGAGTTCAAA+CGG 0.524260 4:+26870911 MsaT019318.1:CDS
CTAGGATCGACCTCGCCAAA+GGG 0.529567 4:-26871366 None:intergenic
ATGTTGTCGCCATTATTCAT+GGG 0.540445 4:-26871288 None:intergenic
GCGGAAGGGTCTACTGAAGA+AGG 0.542122 4:+26870806 MsaT019318.1:CDS
AGAATCATCATCGACAATGA+AGG 0.542377 4:-26870760 None:intergenic
AGTAGATTCTGCAAAACTCT+AGG 0.544749 4:-26871384 None:intergenic
GATGGCCAGATCTCTGGTTG+AGG 0.555241 4:-26870891 None:intergenic
AACATACAATAGAAGGCGGA+AGG 0.566279 4:+26870791 MsaT019318.1:CDS
GTTTGAACTCCCTGTGGTGA+TGG 0.567517 4:-26870909 None:intergenic
TCTAGGATCGACCTCGCCAA+AGG 0.568977 4:-26871367 None:intergenic
AGAGACTTACATGAAAGAAA+GGG 0.577279 4:+26870992 MsaT019318.1:CDS
TATGAACTAAGCACTTTGTG+TGG 0.579542 4:+26870831 MsaT019318.1:CDS
TGGTAGAAGGTTGCAAGCAG+AGG 0.582220 4:+26871173 MsaT019318.1:CDS
CAAAATTCAGAACAATGACT+GGG 0.592691 4:+26870940 MsaT019318.1:CDS
TCAGTAATGAAGAAATGGAA+AGG 0.596923 4:+26871241 MsaT019318.1:CDS
AATGAAGAAATGGAAAGGAT+GGG 0.602949 4:+26871246 MsaT019318.1:CDS
GGCTTGATGATGAATAGTAA+TGG 0.609105 4:+26871330 MsaT019318.1:CDS
TTGCAAGCAGAGGATAATAG+TGG 0.618049 4:+26871183 MsaT019318.1:CDS
TGAGTCGGTGCCCTTTGGCG+AGG 0.619442 4:+26871356 MsaT019318.1:CDS
TTACTGAATGTAAATGGTGG+TGG 0.621024 4:-26871225 None:intergenic
GATTGACCACAAATTGAAGG+AGG 0.630849 4:+26871149 MsaT019318.1:CDS
GCGGAAGAAGAGCAACAAAA+CGG 0.630975 4:+26870962 MsaT019318.1:CDS
GAATAGTAATGGAGATGAGT+CGG 0.650993 4:+26871341 MsaT019318.1:CDS
ACATACAATAGAAGGCGGAA+GGG 0.677825 4:+26870792 MsaT019318.1:CDS
GAGATCTGGCCATCACCACA+GGG 0.678612 4:+26870900 MsaT019318.1:CDS
AAGCAACATACAATAGAAGG+CGG 0.694844 4:+26870787 MsaT019318.1:CDS
TCATTACTGAATGTAAATGG+TGG 0.709945 4:-26871228 None:intergenic
AATTCAGAACAATGACTGGG+CGG 0.724414 4:+26870943 MsaT019318.1:CDS
AGCACCGTTTGAACTCCCTG+TGG 0.787870 4:-26870915 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 26870735 26871413 26870735 ID=MsaG019318
Chr4 mRNA 26870735 26871413 26870735 ID=MsaT019318.1;Parent=MsaG019318
Chr4 exon 26870735 26871013 26870735 ID=MsaT019318.1.exon1;Parent=MsaT019318.1
Chr4 CDS 26870735 26871013 26870735 ID=cds.MsaT019318.1;Parent=MsaT019318.1
Chr4 exon 26871108 26871413 26871108 ID=MsaT019318.1.exon2;Parent=MsaT019318.1
Chr4 CDS 26871108 26871413 26871108 ID=cds.MsaT019318.1;Parent=MsaT019318.1
Gene Sequence

>MsaT019318.1

ATGACTGGGAAAAAGGTGAAACTTGCCTTCATTGTCGATGATGATTCTAGGAAAGCAACATACAATAGAAGGCGGAAGGGTCTACTGAAGAAGGTTTATGAACTAAGCACTTTGTGTGGAGTTGAAGCTTGTGCAGTAGTTTATGGACCCCATGAGCCTCAACCAGAGATCTGGCCATCACCACAGGGAGTTCAAACGGTGCTCTCAAAATTCAGAACAATGACTGGGCGGAAGAAGAGCAACAAAACGGTTGATCAAGAGACTTACATGAAAGAAAGGAATAATATGTCATTGGTTGATTCAAAACATCTTTGTTGGTTGATTGACCACAAATTGAAGGAGGTTGGTAGAAGGTTGCAAGCAGAGGATAATAGTGGTCAAATTCAAATCCAAATGCCACCACCATTTACATTCAGTAATGAAGAAATGGAAAGGATGGGTTCTGGCAATGCTGGAATGCCCATGAATAATGGCGACAACATTGAAAGTCAATCTTTTATGGGCTTGATGATGAATAGTAATGGAGATGAGTCGGTGCCCTTTGGCGAGGTCGATCCTAGAGTTTTGCAGAATCTACTACCTTAA

Protein sequence

>MsaT019318.1

MTGKKVKLAFIVDDDSRKATYNRRRKGLLKKVYELSTLCGVEACAVVYGPHEPQPEIWPSPQGVQTVLSKFRTMTGRKKSNKTVDQETYMKERNNMSLVDSKHLCWLIDHKLKEVGRRLQAEDNSGQIQIQMPPPFTFSNEEMERMGSGNAGMPMNNGDNIESQSFMGLMMNSNGDESVPFGEVDPRVLQNLLP*