AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019319


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019319 MtrunA17_Chr4g0014031 82.231 242 28 1 4 245 3 229 2.57e-135 380
MsaG019319 MtrunA17_Chr4g0014131 79.752 242 36 1 4 245 3 231 1.47e-130 368
MsaG019319 MtrunA17_Chr8g0351751 61.753 251 81 5 4 245 3 247 1.44e-98 287
MsaG019319 MtrunA17_Chr4g0014321 58.367 245 88 4 1 245 1 231 2.35e-94 276
MsaG019319 MtrunA17_Chr4g0013041 58.216 213 59 3 6 218 5 187 9.35e-81 241
MsaG019319 MtrunA17_Chr4g0014121 81.169 154 29 0 4 157 31 184 3.01e-80 239
MsaG019319 MtrunA17_Chr3g0110131 48.133 241 118 4 4 242 3 238 5.74e-62 194
MsaG019319 MtrunA17_Chr3g0107381 54.248 153 69 1 4 156 3 154 6.09e-57 181
MsaG019319 MtrunA17_Chr2g0296831 72.500 120 33 0 4 123 3 122 6.06e-49 157
MsaG019319 MtrunA17_Chr3g0107331 44.099 161 89 1 4 164 2 161 1.15e-42 142
MsaG019319 MtrunA17_Chr3g0107351 42.767 159 90 1 4 162 2 159 6.05e-41 138
MsaG019319 MtrunA17_Chr5g0432691 44.667 150 82 1 6 155 5 153 8.72e-41 137
MsaG019319 MtrunA17_Chr3g0107361 40.881 159 93 1 4 162 2 159 1.24e-38 132
MsaG019319 MtrunA17_Chr3g0107341 41.509 159 92 1 4 162 2 159 2.20e-38 131
MsaG019319 MtrunA17_Chr3g0107461 40.260 154 92 0 4 157 3 156 3.89e-38 130
MsaG019319 MtrunA17_Chr5g0420351 44.156 154 84 2 4 157 3 154 2.08e-37 129
MsaG019319 MtrunA17_Chr2g0283941 40.789 152 90 0 6 157 5 156 8.11e-37 130
MsaG019319 MtrunA17_Chr1g0197461 38.961 154 92 2 4 157 2 153 5.29e-33 117
MsaG019319 MtrunA17_Chr1g0188681 40.260 154 90 2 4 157 3 154 5.38e-33 117
MsaG019319 MtrunA17_Chr1g0197321 38.994 159 95 2 1 159 1 157 1.93e-32 116
MsaG019319 MtrunA17_Chr1g0197261 39.103 156 93 2 4 159 3 156 4.62e-32 115
MsaG019319 MtrunA17_Chr3g0105791 39.416 137 83 0 30 166 1 137 2.40e-31 112
MsaG019319 MtrunA17_Chr7g0218151 42.208 154 87 2 4 157 3 154 1.63e-30 111
MsaG019319 MtrunA17_Chr1g0188661 42.208 154 87 2 4 157 3 154 1.82e-30 110
MsaG019319 MtrunA17_Chr1g0193311 42.857 154 86 2 4 157 3 154 2.07e-30 110
MsaG019319 MtrunA17_Chr4g0031421 39.416 137 83 0 30 166 1 137 2.77e-30 110
MsaG019319 MtrunA17_Chr4g0012761 40.909 154 89 2 4 157 3 154 7.11e-29 107
MsaG019319 MtrunA17_Chr1g0188651 42.208 154 85 2 4 157 3 152 1.52e-27 103
MsaG019319 MtrunA17_Chr3g0106421 39.844 128 77 0 30 157 1 128 9.42e-27 103
MsaG019319 MtrunA17_Chr4g0008701 38.462 156 80 2 3 158 5 144 5.13e-25 102
MsaG019319 MtrunA17_Chr7g0267601 37.821 156 81 2 3 158 5 144 5.50e-25 102
MsaG019319 MtrunA17_Chr2g0296851 59.794 97 38 1 75 170 1 97 7.90e-25 94.4
MsaG019319 MtrunA17_Chr3g0128721 29.016 193 126 2 3 195 5 186 1.36e-24 102
MsaG019319 MtrunA17_Chr2g0296861 34.437 151 98 1 7 157 6 155 4.74e-22 89.0
MsaG019319 MtrunA17_Chr3g0106501 30.769 130 90 0 30 159 1 130 2.81e-17 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019319 AT5G48670.1 53.165 158 73 1 4 161 3 159 3.94e-44 151
MsaG019319 AT1G65300.1 40.217 184 96 3 4 187 2 171 9.40e-40 139
MsaG019319 AT5G26630.1 41.872 203 101 3 4 204 3 190 7.57e-39 134
MsaG019319 AT1G65330.1 40.541 185 96 2 4 188 2 172 1.19e-38 136
MsaG019319 AT5G26650.1 40.244 164 97 1 6 169 3 165 2.78e-35 129
MsaG019319 AT5G27960.1 39.634 164 98 1 6 169 3 165 1.74e-34 126
MsaG019319 AT1G31630.1 34.615 182 104 3 4 185 2 168 4.12e-33 123
MsaG019319 AT1G31640.1 38.411 151 91 2 4 154 2 150 4.40e-30 117
MsaG019319 AT5G27810.1 53.763 93 43 0 30 122 1 93 5.23e-29 106
MsaG019319 AT3G05860.2 38.514 148 90 1 4 151 3 149 2.87e-28 107
MsaG019319 AT3G05860.1 38.514 148 90 1 4 151 3 149 9.81e-28 107
MsaG019319 AT3G05860.3 38.514 148 90 1 4 151 3 149 1.04e-27 106
MsaG019319 AT2G28700.1 35.758 165 101 2 4 166 3 164 3.66e-26 104
MsaG019319 AT5G26580.1 35.897 156 84 3 1 156 1 140 2.69e-24 99.8
MsaG019319 AT1G22590.2 31.210 157 103 2 1 157 1 152 3.79e-23 92.4
MsaG019319 AT5G06500.1 34.868 152 97 2 4 155 3 152 4.74e-19 83.6
MsaG019319 AT2G40210.1 39.098 133 79 2 4 135 3 134 3.09e-17 80.5
MsaG019319 AT5G58890.1 37.795 127 68 5 7 124 6 130 2.00e-14 71.6
MsaG019319 AT5G55690.1 41.772 79 38 3 1 72 1 78 6.60e-11 61.2
MsaG019319 AT5G55690.2 41.772 79 38 3 1 72 1 78 6.60e-11 61.2

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAACAAAGAAAAGAAAAT+AGG 0.231520 4:+26873057 MsaT019319.1:CDS
GAGTGAAGAAATGGCAATTA+TGG 0.233693 4:+26873319 MsaT019319.1:CDS
TCAATTTCTCTTCAGCCTTC+AGG 0.259829 4:-26873034 None:intergenic
CCATTTGGTGAGGTCAGTTC+TGG 0.275094 4:+26873440 MsaT019319.1:CDS
AACGAAGGGTCTATTGAAAA+AGG 0.335070 4:+26872824 MsaT019319.1:CDS
ATGAGCCTCAACCTGAGATT+TGG 0.345571 4:+26872904 MsaT019319.1:CDS
ATAATGTCATCATCATTAAT+AGG 0.375800 4:-26873362 None:intergenic
GAAGCCTTTGCCATAGTGTA+TGG 0.388094 4:+26872876 MsaT019319.1:CDS
TCGCCATTTCGAGTTGGACT+TGG 0.402057 4:-26873262 None:intergenic
TATGCAAAGTCAATTATTGA+TGG 0.408286 4:+26873382 MsaT019319.1:CDS
ATCTCAGGTTGAGGCTCATA+AGG 0.418788 4:-26872900 None:intergenic
GAAACAAAGAAAAGAAAATA+GGG 0.419680 4:+26873058 MsaT019319.1:CDS
GTGAGGTCAGTTCTGGACTT+TGG 0.421386 4:+26873447 MsaT019319.1:CDS
AGTGAAGAAATGGCAATTAT+GGG 0.424565 4:+26873320 MsaT019319.1:CDS
GAAGGATGTTAGTAGACGTT+TGG 0.425640 4:+26873193 MsaT019319.1:CDS
GGTCTGATTTACTACCTTGA+AGG 0.436579 4:+26873468 MsaT019319.1:CDS
AAACCAAGTCCAACTCGAAA+TGG 0.452175 4:+26873259 MsaT019319.1:CDS
AGCATCTAGGAGTGAAGAAA+TGG 0.457574 4:+26873310 MsaT019319.1:CDS
AACCTTACTTCTTCTACCCA+TGG 0.464315 4:-26872748 None:intergenic
CTCATAAGGTCCATACACTA+TGG 0.467279 4:-26872886 None:intergenic
TTATGGGTCATGGCCATGTT+GGG 0.476409 4:+26873336 MsaT019319.1:CDS
TCAATTATTGATGGACTCAA+TGG 0.482426 4:+26873391 MsaT019319.1:CDS
GTTGATTGACCACAATTTGA+AGG 0.487410 4:+26873175 MsaT019319.1:CDS
TCTGGTAAAGGCCAAATCTC+AGG 0.493519 4:-26872915 None:intergenic
AACCATGGGTAGAAGAAGTA+AGG 0.501192 4:+26872746 None:intergenic
TTAGTAGACGTTTGGAAGCA+TGG 0.506023 4:+26873201 MsaT019319.1:CDS
TGGATATGAGTTATCTTGCT+TGG 0.509186 4:+26873153 MsaT019319.1:CDS
CCAGAACTGACCTCACCAAA+TGG 0.510928 4:-26873440 None:intergenic
GATTGGACACCCTCTGGTAA+AGG 0.514238 4:-26872927 None:intergenic
TGCAACATCATTGACAATGA+AGG 0.517037 4:-26872778 None:intergenic
ATGAGTCAATGTCTTAATGA+AGG 0.517500 4:+26873101 MsaT019319.1:CDS
CAATGATCAAAATCAAATCA+TGG 0.521352 4:+26873229 MsaT019319.1:CDS
AGCACAGATTGGACACCCTC+TGG 0.522070 4:-26872933 None:intergenic
CACCACTTCTAGCAGCATCT+AGG 0.525777 4:+26873297 MsaT019319.1:CDS
TTTGTGTCAAGAAAGTCTCT+TGG 0.525855 4:-26873007 None:intergenic
AGAGACTTTCTTGACACAAA+GGG 0.533444 4:+26873010 MsaT019319.1:CDS
GTGCAAGAGACAGTGCCATT+TGG 0.542398 4:+26873425 MsaT019319.1:CDS
TACTAACATCCTTCAAATTG+TGG 0.547844 4:-26873184 None:intergenic
GAAATGGCAATTATGGGTCA+TGG 0.549964 4:+26873326 MsaT019319.1:CDS
TAGTAGACGTTTGGAAGCAT+GGG 0.553921 4:+26873202 MsaT019319.1:CDS
GAGATTTGGCCTTTACCAGA+GGG 0.563951 4:+26872918 MsaT019319.1:CDS
ATTATGGGTCATGGCCATGT+TGG 0.565284 4:+26873335 MsaT019319.1:CDS
CTTGACACAAAGGGTCCTGA+AGG 0.570745 4:+26873019 MsaT019319.1:CDS
CTCCTAGATGCTGCTAGAAG+TGG 0.571630 4:-26873299 None:intergenic
TTGTTGTCGCCATTTCGAGT+TGG 0.584691 4:-26873268 None:intergenic
CATTAATAGGTATCCCAACA+TGG 0.589301 4:-26873349 None:intergenic
TCATTGTCAATGATGTTGCA+AGG 0.595963 4:+26872781 MsaT019319.1:CDS
AAAGGCCAAATCTCAGGTTG+AGG 0.605277 4:-26872909 None:intergenic
CTAGATGCTGCTAGAAGTGG+TGG 0.605478 4:-26873296 None:intergenic
TTGATGGACTCAATGGTGGC+TGG 0.607204 4:+26873398 MsaT019319.1:CDS
AAAGGCTTCAATGCCACAGA+GGG 0.618725 4:-26872862 None:intergenic
ATCATACAAGAAAAGAACGA+AGG 0.618898 4:+26872809 MsaT019319.1:CDS
TCATACAAGAAAAGAACGAA+GGG 0.620354 4:+26872810 MsaT019319.1:CDS
AAGAGACTTTCTTGACACAA+AGG 0.620991 4:+26873009 MsaT019319.1:CDS
TGAGATTTGGCCTTTACCAG+AGG 0.625137 4:+26872917 MsaT019319.1:CDS
GATGCTGCTAGAAGTGGTGG+TGG 0.633563 4:-26873293 None:intergenic
AGGTCCATACACTATGGCAA+AGG 0.638234 4:-26872880 None:intergenic
AGAGACAGTGCCATTTGGTG+AGG 0.639188 4:+26873430 MsaT019319.1:CDS
ATTATTGATGGACTCAATGG+TGG 0.666274 4:+26873394 MsaT019319.1:CDS
GCATGACAATATGTCAACAA+TGG 0.671130 4:+26873133 MsaT019319.1:CDS
GCTGAACTTAGCACCCTCTG+TGG 0.677042 4:+26872849 MsaT019319.1:CDS
CAAAGGCTTCAATGCCACAG+AGG 0.706392 4:-26872863 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 26872750 26873487 26872750 ID=MsaG019319
Chr4 mRNA 26872750 26873487 26872750 ID=MsaT019319.1;Parent=MsaG019319
Chr4 exon 26872750 26873487 26872750 ID=MsaT019319.1.exon1;Parent=MsaT019319.1
Chr4 CDS 26872750 26873487 26872750 ID=cds.MsaT019319.1;Parent=MsaT019319.1
Gene Sequence

>MsaT019319.1

ATGGGTAGAAGAAGTAAGGTTAAACTTGCCTTCATTGTCAATGATGTTGCAAGGAAAGCATCATACAAGAAAAGAACGAAGGGTCTATTGAAAAAGGTTGCTGAACTTAGCACCCTCTGTGGCATTGAAGCCTTTGCCATAGTGTATGGACCTTATGAGCCTCAACCTGAGATTTGGCCTTTACCAGAGGGTGTCCAATCTGTGCTCTCAAAATTCATGACGATGCACGAGTTTCAAAAGTGCAAGAAAATGATGAACCAAGAGACTTTCTTGACACAAAGGGTCCTGAAGGCTGAAGAGAAATTGAAGAAACAAAGAAAAGAAAATAGGGAGCAAGAGATGACAATACTCATGAGTCAATGTCTTAATGAAGGTAAAGTTGTGCATGACAATATGTCAACAATGGATATGAGTTATCTTGCTTGGTTGATTGACCACAATTTGAAGGATGTTAGTAGACGTTTGGAAGCATGGGATAACAATGATCAAAATCAAATCATGGCTATTCAAAACCAAGTCCAACTCGAAATGGCGACAACAATACCACCACCACTTCTAGCAGCATCTAGGAGTGAAGAAATGGCAATTATGGGTCATGGCCATGTTGGGATACCTATTAATGATGATGACATTATGCAAAGTCAATTATTGATGGACTCAATGGTGGCTGGTAATGTGCAAGAGACAGTGCCATTTGGTGAGGTCAGTTCTGGACTTTGGTCTGATTTACTACCTTGA

Protein sequence

>MsaT019319.1

MGRRSKVKLAFIVNDVARKASYKKRTKGLLKKVAELSTLCGIEAFAIVYGPYEPQPEIWPLPEGVQSVLSKFMTMHEFQKCKKMMNQETFLTQRVLKAEEKLKKQRKENREQEMTILMSQCLNEGKVVHDNMSTMDMSYLAWLIDHNLKDVSRRLEAWDNNDQNQIMAIQNQVQLEMATTIPPPLLAASRSEEMAIMGHGHVGIPINDDDIMQSQLLMDSMVAGNVQETVPFGEVSSGLWSDLLP*