AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019321

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019321 MtrunA17_Chr4g0014031 89.313 131 14 0 1 131 1 131 5.36e-78 230
MsaG019321 MtrunA17_Chr4g0014121 82.443 131 23 0 1 131 29 159 1.29e-69 207
MsaG019321 MtrunA17_Chr4g0014131 83.969 131 21 0 1 131 1 131 5.22e-69 207
MsaG019321 MtrunA17_Chr4g0013041 67.939 131 40 1 1 131 1 129 1.15e-60 185
MsaG019321 MtrunA17_Chr4g0014321 66.412 131 44 0 1 131 1 131 1.77e-59 183
MsaG019321 MtrunA17_Chr8g0351751 69.925 133 38 1 1 131 1 133 1.32e-58 181
MsaG019321 MtrunA17_Chr3g0107381 58.730 126 52 0 1 126 1 126 7.20e-53 166
MsaG019321 MtrunA17_Chr2g0296831 72.131 122 34 0 1 122 1 122 7.85e-53 162
MsaG019321 MtrunA17_Chr3g0110131 64.341 129 46 0 1 129 1 129 8.92e-52 164
MsaG019321 MtrunA17_Chr3g0107331 52.101 119 57 0 3 121 2 120 1.73e-42 137
MsaG019321 MtrunA17_Chr5g0432691 49.612 129 65 0 1 129 1 129 1.98e-41 135
MsaG019321 MtrunA17_Chr3g0107361 48.739 119 61 0 3 121 2 120 1.03e-39 130
MsaG019321 MtrunA17_Chr3g0107351 47.619 126 66 0 3 128 2 127 1.24e-39 130
MsaG019321 MtrunA17_Chr3g0107341 47.619 126 66 0 3 128 2 127 1.96e-39 130
MsaG019321 MtrunA17_Chr3g0107461 47.541 122 64 0 1 122 1 122 1.23e-38 127
MsaG019321 MtrunA17_Chr5g0420351 48.760 121 61 1 1 121 1 120 5.93e-38 125
MsaG019321 MtrunA17_Chr1g0188681 45.968 124 65 2 1 123 1 123 1.77e-36 122
MsaG019321 MtrunA17_Chr2g0283941 46.281 121 65 0 1 121 1 121 3.65e-36 124
MsaG019321 MtrunA17_Chr1g0197321 41.985 131 75 1 1 131 1 130 8.68e-34 115
MsaG019321 MtrunA17_Chr1g0197461 42.636 129 72 2 3 131 2 128 1.60e-33 114
MsaG019321 MtrunA17_Chr1g0197261 42.636 129 73 1 1 129 1 128 3.32e-33 114
MsaG019321 MtrunA17_Chr7g0218151 44.961 129 70 1 1 129 1 128 2.68e-31 109
MsaG019321 MtrunA17_Chr1g0188661 44.961 129 70 1 1 129 1 128 1.01e-30 107
MsaG019321 MtrunA17_Chr3g0105791 50.538 93 46 0 29 121 1 93 1.15e-30 107
MsaG019321 MtrunA17_Chr1g0193311 49.573 117 58 1 1 117 1 116 4.73e-30 105
MsaG019321 MtrunA17_Chr4g0012761 43.411 129 72 1 1 129 1 128 7.03e-30 105
MsaG019321 MtrunA17_Chr1g0188651 51.304 115 55 1 1 115 1 114 7.84e-30 105
MsaG019321 MtrunA17_Chr4g0031421 49.462 93 47 0 29 121 1 93 3.36e-28 100
MsaG019321 MtrunA17_Chr7g0267601 39.370 127 77 0 3 129 6 132 9.52e-27 103
MsaG019321 MtrunA17_Chr4g0008701 39.370 127 77 0 3 129 6 132 2.71e-26 102
MsaG019321 MtrunA17_Chr3g0128721 35.433 127 82 0 3 129 6 132 3.54e-24 96.3
MsaG019321 MtrunA17_Chr2g0296861 40.000 95 57 0 1 95 1 95 4.23e-24 90.9
MsaG019321 MtrunA17_Chr3g0106421 47.191 89 47 0 29 117 1 89 3.31e-23 90.1
MsaG019321 MtrunA17_Chr3g0106501 41.573 89 52 0 29 117 1 89 1.03e-19 79.3
MsaG019321 MtrunA17_Chr6g0457851 35.398 113 66 4 1 107 11 122 1.10e-13 66.2
MsaG019321 MtrunA17_Chr6g0449861 35.398 113 66 4 1 107 9 120 1.27e-13 66.2
MsaG019321 MtrunA17_Chr1g0212151 32.743 113 69 3 1 107 1 112 1.74e-13 65.5
MsaG019321 MtrunA17_Chr6g0449851 36.283 113 65 4 1 107 1 112 1.86e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019321 AT5G48670.1 59.504 121 49 0 1 121 1 121 1.27e-40 137
MsaG019321 AT1G65330.1 51.261 119 58 0 3 121 2 120 3.53e-38 130
MsaG019321 AT1G65300.1 48.739 119 61 0 3 121 2 120 1.99e-37 128
MsaG019321 AT5G27960.1 48.361 122 62 1 5 126 3 123 1.50e-35 124
MsaG019321 AT5G26650.1 48.361 122 62 1 5 126 3 123 1.92e-35 125
MsaG019321 AT5G26630.1 52.344 128 61 0 1 128 1 128 5.83e-33 115
MsaG019321 AT5G27810.1 54.839 93 42 0 29 121 1 93 3.02e-32 110
MsaG019321 AT3G05860.2 42.478 113 65 0 1 113 1 113 6.71e-31 110
MsaG019321 AT1G31630.1 41.935 124 70 2 3 126 2 123 9.31e-31 112
MsaG019321 AT3G05860.3 42.478 113 65 0 1 113 1 113 2.36e-30 109
MsaG019321 AT3G05860.1 42.478 113 65 0 1 113 1 113 3.29e-30 109
MsaG019321 AT1G31640.1 42.857 119 67 1 3 121 2 119 7.98e-30 112
MsaG019321 AT2G28700.1 42.105 133 74 2 1 130 1 133 1.35e-28 106
MsaG019321 AT1G22590.2 36.207 116 74 0 1 116 1 116 3.29e-25 94.0
MsaG019321 AT5G26580.1 38.095 126 63 2 1 126 1 111 1.51e-24 96.3
MsaG019321 AT5G06500.1 37.209 129 80 1 1 129 1 128 8.34e-21 84.7
MsaG019321 AT2G40210.1 39.535 129 77 1 1 129 1 128 4.05e-20 84.7
MsaG019321 AT5G58890.1 41.593 113 55 5 6 109 6 116 8.15e-17 74.7
MsaG019321 AT5G55690.1 42.308 78 38 2 1 71 1 78 5.80e-14 67.0
MsaG019321 AT5G55690.2 42.308 78 38 2 1 71 1 78 5.80e-14 67.0
MsaG019321 AT5G27090.1 33.333 90 59 1 7 95 23 112 5.12e-11 57.8
MsaG019321 AT5G27944.1 38.356 73 44 1 24 95 41 113 5.80e-11 58.2

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAACAAAGAAAAGAAAAT+AGG 0.231520 4:+26901931 MsaT019321.1:CDS
AAGAAAGGGTCTATTGAAAA+AGG 0.312767 4:+26901698 MsaT019321.1:CDS
ATCTCTGGCTGAGGCTCATA+AGG 0.350996 4:-26901774 None:intergenic
ATGAGCCTCAGCCAGAGATT+TGG 0.374512 4:+26901778 MsaT019321.1:CDS
TCTGGTGAAGGCCAAATCTC+TGG 0.413104 4:-26901789 None:intergenic
GAAACAAAGAAAAGAAAATA+GGG 0.419680 4:+26901932 MsaT019321.1:CDS
CACAACCATGGCTAGAAGTA+AGG 0.461347 4:+26901620 None:intergenic
CTCATAAGGGCCATACACTA+TGG 0.468858 4:-26901760 None:intergenic
ATCATACAAGAAAAGAAGAA+AGG 0.473094 4:+26901683 MsaT019321.1:CDS
CTTGACACAAAGGGTTCTGA+AGG 0.478789 4:+26901893 MsaT019321.1:CDS
TGCGGCATCATTGACAATGA+AGG 0.507200 4:-26901652 None:intergenic
AGAGACTTTCTTGACACAAA+GGG 0.517022 4:+26901884 MsaT019321.1:CDS
TTTGTGTCAAGAAAGTCTCT+TGG 0.525855 4:-26901881 None:intergenic
TCATACAAGAAAAGAAGAAA+GGG 0.526600 4:+26901684 MsaT019321.1:CDS
TTTAACCTTACTTCTAGCCA+TGG 0.533701 4:-26901625 None:intergenic
ATATCTCATTGTCTCAATGA+AGG 0.546787 4:+26901975 MsaT019321.1:CDS
GAAGCCTGTGCCATAGTGTA+TGG 0.559714 4:+26901750 MsaT019321.1:CDS
GAAGGCCAAATCTCTGGCTG+AGG 0.560020 4:-26901783 None:intergenic
TCTCTGGCTGAGGCTCATAA+GGG 0.571102 4:-26901773 None:intergenic
GAGATTTGGCCTTCACCAGA+GGG 0.580805 4:+26901792 MsaT019321.1:CDS
ACAGGCTTCAATGCCACAGA+GGG 0.601811 4:-26901736 None:intergenic
GATTTGGCCTTCACCAGAGG+GGG 0.601928 4:+26901794 MsaT019321.1:CDS
CTTGTATGATTCTTTCCTTG+CGG 0.605657 4:-26901670 None:intergenic
AAGAGACTTTCTTGACACAA+AGG 0.607804 4:+26901883 MsaT019321.1:CDS
AGAGATTTGGCCTTCACCAG+AGG 0.617598 4:+26901791 MsaT019321.1:CDS
AGGGCCATACACTATGGCAC+AGG 0.641363 4:-26901754 None:intergenic
TCATTGTCAATGATGCCGCA+AGG 0.664783 4:+26901655 MsaT019321.1:CDS
GCTGAACTTAGCACCCTCTG+TGG 0.677042 4:+26901723 MsaT019321.1:CDS
CACAGGCTTCAATGCCACAG+AGG 0.696692 4:-26901737 None:intergenic
AGATTTGGCCTTCACCAGAG+GGG 0.703414 4:+26901793 MsaT019321.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 26901627 26902022 26901627 ID=MsaG019321
Chr4 mRNA 26901627 26902022 26901627 ID=MsaT019321.1;Parent=MsaG019321
Chr4 exon 26901627 26902022 26901627 ID=MsaT019321.1.exon1;Parent=MsaT019321.1
Chr4 CDS 26901627 26902022 26901627 ID=cds.MsaT019321.1;Parent=MsaT019321.1
Gene Sequence

>MsaT019321.1

ATGGCTAGAAGTAAGGTTAAACTTGCCTTCATTGTCAATGATGCCGCAAGGAAAGAATCATACAAGAAAAGAAGAAAGGGTCTATTGAAAAAGGTTGCTGAACTTAGCACCCTCTGTGGCATTGAAGCCTGTGCCATAGTGTATGGCCCTTATGAGCCTCAGCCAGAGATTTGGCCTTCACCAGAGGGGGTCCAATCCGTGCTCTCAAAATTCATGACGATGCACGAGTTTCAAAAGTGCAAGAAAATGATGAACCAAGAGACTTTCTTGACACAAAGGGTTCTGAAGGCTGAAGAGAAATTGAAGAAACAAAGAAAAGAAAATAGGGAGCAAGAGATGACAATACTCATATCTCATTGTCTCAATGAAGGTAGAGTTGTGCACGACAATATGTAA
Protein sequence

>MsaT019321.1

MARSKVKLAFIVNDAARKESYKKRRKGLLKKVAELSTLCGIEACAIVYGPYEPQPEIWPSPEGVQSVLSKFMTMHEFQKCKKMMNQETFLTQRVLKAEEKLKKQRKENREQEMTILISHCLNEGRVVHDNM*