AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019322


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019322 MtrunA17_Chr4g0014321 79.365 126 26 0 1 126 1 126 1.68e-66 201
MsaG019322 MtrunA17_Chr4g0013041 76.860 121 26 1 1 121 1 119 6.19e-61 186
MsaG019322 MtrunA17_Chr8g0351751 66.667 123 39 1 1 121 1 123 1.45e-48 156
MsaG019322 MtrunA17_Chr4g0014031 66.942 121 40 0 1 121 1 121 2.35e-48 155
MsaG019322 MtrunA17_Chr4g0014121 69.421 121 37 0 1 121 29 149 2.48e-48 154
MsaG019322 MtrunA17_Chr3g0107381 55.303 132 56 1 1 132 1 129 3.67e-48 154
MsaG019322 MtrunA17_Chr2g0296831 66.116 121 41 0 1 121 1 121 8.81e-48 150
MsaG019322 MtrunA17_Chr3g0110131 64.463 121 43 0 1 121 1 121 3.18e-47 152
MsaG019322 MtrunA17_Chr4g0014131 65.289 121 42 0 1 121 1 121 3.34e-44 144
MsaG019322 MtrunA17_Chr3g0107331 50.000 120 60 0 3 122 2 121 1.29e-41 135
MsaG019322 MtrunA17_Chr5g0420351 47.107 121 63 1 1 121 1 120 4.59e-40 131
MsaG019322 MtrunA17_Chr3g0107361 46.512 129 67 2 3 130 2 129 6.55e-38 126
MsaG019322 MtrunA17_Chr1g0197261 44.444 126 69 1 1 126 1 125 8.08e-37 123
MsaG019322 MtrunA17_Chr3g0107341 47.899 119 62 0 3 121 2 120 1.72e-36 122
MsaG019322 MtrunA17_Chr3g0107351 45.161 124 68 0 3 126 2 125 1.39e-35 120
MsaG019322 MtrunA17_Chr1g0188681 43.802 121 67 1 1 121 1 120 1.88e-34 117
MsaG019322 MtrunA17_Chr1g0197321 41.600 125 72 1 1 125 1 124 4.43e-34 116
MsaG019322 MtrunA17_Chr1g0193311 48.305 118 60 1 1 118 1 117 9.25e-33 113
MsaG019322 MtrunA17_Chr7g0218151 45.763 118 63 1 1 118 1 117 7.92e-32 110
MsaG019322 MtrunA17_Chr1g0197461 41.129 124 72 1 3 126 2 124 1.19e-31 110
MsaG019322 MtrunA17_Chr5g0432691 43.651 126 71 0 1 126 1 126 3.68e-31 109
MsaG019322 MtrunA17_Chr3g0107461 40.650 123 73 0 1 123 1 123 6.10e-31 108
MsaG019322 MtrunA17_Chr4g0012761 45.763 118 63 1 1 118 1 117 3.81e-30 106
MsaG019322 MtrunA17_Chr2g0283941 43.089 123 70 0 1 123 1 123 5.84e-30 108
MsaG019322 MtrunA17_Chr1g0188661 41.525 118 68 1 1 118 1 117 2.26e-27 99.8
MsaG019322 MtrunA17_Chr3g0105791 43.878 98 55 0 29 126 1 98 4.45e-24 90.5
MsaG019322 MtrunA17_Chr1g0188651 43.590 117 65 1 1 117 1 116 4.51e-24 90.9
MsaG019322 MtrunA17_Chr4g0008701 37.705 122 73 1 1 119 1 122 4.09e-23 93.6
MsaG019322 MtrunA17_Chr7g0267601 36.885 122 74 1 1 119 1 122 5.72e-23 93.2
MsaG019322 MtrunA17_Chr4g0031421 41.837 98 57 0 29 126 1 98 1.48e-22 86.7
MsaG019322 MtrunA17_Chr2g0296861 36.842 95 60 0 1 95 1 95 1.37e-21 84.7
MsaG019322 MtrunA17_Chr3g0106421 42.268 97 56 0 29 125 1 97 5.72e-19 79.3
MsaG019322 MtrunA17_Chr3g0128721 35.484 124 75 2 3 125 6 125 2.51e-18 80.1
MsaG019322 MtrunA17_Chr3g0106501 37.209 86 54 0 29 114 1 86 4.99e-16 70.1
MsaG019322 MtrunA17_Chr6g0457851 38.462 104 57 4 1 98 11 113 4.95e-14 67.4
MsaG019322 MtrunA17_Chr1g0212151 34.188 117 62 4 1 103 1 116 3.37e-13 65.1
MsaG019322 MtrunA17_Chr6g0449851 37.288 118 59 5 1 104 1 117 4.51e-12 62.0
MsaG019322 MtrunA17_Chr6g0449861 36.441 118 60 5 1 104 9 125 5.39e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019322 AT5G48670.1 59.016 122 50 0 1 122 1 122 3.29e-43 145
MsaG019322 AT5G27960.1 46.610 118 63 0 4 121 2 119 1.22e-33 120
MsaG019322 AT5G26650.1 46.610 118 63 0 4 121 2 119 1.38e-33 120
MsaG019322 AT5G27810.1 53.191 94 44 0 29 122 1 94 2.62e-33 114
MsaG019322 AT1G65300.1 46.218 119 64 0 3 121 2 120 4.49e-33 117
MsaG019322 AT1G65330.1 46.218 119 64 0 3 121 2 120 1.55e-32 116
MsaG019322 AT3G05860.2 41.322 121 71 0 1 121 1 121 2.54e-31 111
MsaG019322 AT5G26630.1 48.760 121 62 0 1 121 1 121 1.20e-30 110
MsaG019322 AT3G05860.3 41.322 121 71 0 1 121 1 121 1.30e-30 110
MsaG019322 AT3G05860.1 41.322 121 71 0 1 121 1 121 1.72e-30 110
MsaG019322 AT1G31640.1 38.655 119 72 1 3 121 2 119 1.48e-28 108
MsaG019322 AT1G31630.1 38.655 119 72 1 3 121 2 119 7.31e-27 102
MsaG019322 AT2G28700.1 33.562 146 85 1 1 134 1 146 1.55e-24 96.3
MsaG019322 AT1G22590.2 34.483 116 76 0 1 116 1 116 1.04e-23 90.5
MsaG019322 AT5G26580.1 38.843 121 60 1 1 121 1 107 3.82e-23 92.8
MsaG019322 AT5G06500.1 39.167 120 73 0 1 120 1 120 5.15e-21 85.5
MsaG019322 AT2G40210.1 42.017 119 69 0 1 119 1 119 4.05e-20 85.1
MsaG019322 AT5G55690.1 43.038 79 38 2 1 72 1 79 1.46e-12 63.5
MsaG019322 AT5G55690.2 43.038 79 38 2 1 72 1 79 1.46e-12 63.5
MsaG019322 AT5G58890.1 37.374 99 54 3 1 92 1 98 7.76e-12 61.6

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTTGTGCCATAGTTTA+TGG 0.132446 4:+26932069 MsaT019322.1:CDS
CACAACAATGACTAGGAAAA+AGG 0.228363 4:+26931939 None:intergenic
TCATTGTAGATGATGATTCT+AGG 0.286481 4:+26931974 MsaT019322.1:CDS
TATGCAGAATAATATGTCAT+TGG 0.399656 4:+26932322 MsaT019322.1:CDS
GGAAAGCAACATACAATAGA+AGG 0.409123 4:+26931995 MsaT019322.1:CDS
TGTGGTGACGGCCAGATCTC+TGG 0.431268 4:-26932108 None:intergenic
CATGAAAGAAAGGGTTCTAA+AGG 0.457088 4:+26932212 MsaT019322.1:CDS
AGAGATCTGGCCGTCACCAC+AGG 0.458628 4:+26932110 MsaT019322.1:CDS
AAGAGACTTACATGAAAGAA+AGG 0.463147 4:+26932202 MsaT019322.1:CDS
ATCTCTGGTTGAGGCTCGTA+GGG 0.464949 4:-26932093 None:intergenic
TTGATTCAAAACATCTTTGT+TGG 0.470119 4:+26932345 MsaT019322.1:CDS
ACGAGCCTCAACCAGAGATC+TGG 0.471522 4:+26932097 MsaT019322.1:CDS
CTCGTAGGGTCCATAAACTA+TGG 0.509841 4:-26932079 None:intergenic
GATCTCTGGTTGAGGCTCGT+AGG 0.522551 4:-26932094 None:intergenic
AGAATCATCATCTACAATGA+AGG 0.528174 4:-26931971 None:intergenic
GACGGCCAGATCTCTGGTTG+AGG 0.531549 4:-26932102 None:intergenic
AAAATTCAGAACAATGAATG+TGG 0.538041 4:+26932152 MsaT019322.1:CDS
GCGGAAGGGTCTACTGAAGA+AGG 0.542122 4:+26932017 MsaT019322.1:CDS
AAATTCAGAACAATGAATGT+GGG 0.550450 4:+26932153 MsaT019322.1:CDS
AAGGCATGATAACAAGGAGA+TGG 0.564923 4:+26932257 MsaT019322.1:CDS
AACATACAATAGAAGGCGGA+AGG 0.566279 4:+26932002 MsaT019322.1:CDS
AGAGACTTACATGAAAGAAA+GGG 0.577279 4:+26932203 MsaT019322.1:CDS
GTTTGGACTCCCTGTGGTGA+CGG 0.587157 4:-26932120 None:intergenic
GATACAAAGGCATGATAACA+AGG 0.593660 4:+26932251 MsaT019322.1:CDS
AAGAGAAACTAAAGATACAA+AGG 0.596350 4:+26932238 MsaT019322.1:CDS
AATTCAGAACAATGAATGTG+GGG 0.603139 4:+26932154 MsaT019322.1:CDS
GAGATCTGGCCGTCACCACA+GGG 0.642312 4:+26932111 MsaT019322.1:CDS
ATTCAGAACAATGAATGTGG+GGG 0.650757 4:+26932155 MsaT019322.1:CDS
AGCACTGTTTGGACTCCCTG+TGG 0.665566 4:-26932126 None:intergenic
ACATACAATAGAAGGCGGAA+GGG 0.677825 4:+26932003 MsaT019322.1:CDS
AAGCAACATACAATAGAAGG+CGG 0.694844 4:+26931998 MsaT019322.1:CDS
TATGAACTAAGCACTCTGTG+TGG 0.701795 4:+26932042 MsaT019322.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 26931946 26932371 26931946 ID=MsaG019322
Chr4 mRNA 26931946 26932371 26931946 ID=MsaT019322.1;Parent=MsaG019322
Chr4 exon 26931946 26932371 26931946 ID=MsaT019322.1.exon1;Parent=MsaT019322.1
Chr4 CDS 26931946 26932371 26931946 ID=cds.MsaT019322.1;Parent=MsaT019322.1
Gene Sequence

>MsaT019322.1

ATGACTAGGAAAAAGGTGAAACTTGCCTTCATTGTAGATGATGATTCTAGGAAAGCAACATACAATAGAAGGCGGAAGGGTCTACTGAAGAAGGTTTATGAACTAAGCACTCTGTGTGGAGTTATAGCTTGTGCCATAGTTTATGGACCCTACGAGCCTCAACCAGAGATCTGGCCGTCACCACAGGGAGTCCAAACAGTGCTCTCAAAATTCAGAACAATGAATGTGGGGGAGAAGAGCAACAAAACGGTTGATCAAGAGACTTACATGAAAGAAAGGGTTCTAAAGGCTAAAGAGAAACTAAAGATACAAAGGCATGATAACAAGGAGATGGAGATGACAATGCTCATGATTCAATGTCTTTTGACAATAAATTTATGCAGAATAATATGTCATTGGTTGATTCAAAACATCTTTGTTGGTTGA

Protein sequence

>MsaT019322.1

MTRKKVKLAFIVDDDSRKATYNRRRKGLLKKVYELSTLCGVIACAIVYGPYEPQPEIWPSPQGVQTVLSKFRTMNVGEKSNKTVDQETYMKERVLKAKEKLKIQRHDNKEMEMTMLMIQCLLTINLCRIICHWLIQNIFVG*