Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG019452 | XP_039689122.1 | 96.651 | 209 | 7 | 0 | 2 | 210 | 72 | 280 | 3.90e-148 | 423 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG019452 | tr|A0A396I1U4|A0A396I1U4_MEDTR | 96.651 | 209 | 7 | 0 | 2 | 210 | 72 | 280 | 1.86e-148 | 423 |
Gene ID | Type | Classification |
---|---|---|
MsaG019452 | TR | GNAT |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000107 | MsaG019452 | 0.806241 | 1.533522e-49 | 2.393550e-47 |
MsaG000464 | MsaG019452 | 0.805391 | 2.309277e-49 | 3.532894e-47 |
MsaG003564 | MsaG019452 | 0.801081 | 1.784448e-48 | 2.472179e-46 |
MsaG003768 | MsaG019452 | 0.837339 | 1.010331e-56 | 3.631031e-54 |
MsaG004798 | MsaG019452 | 0.801193 | 1.692756e-48 | 2.351159e-46 |
MsaG005107 | MsaG019452 | 0.839113 | 3.544639e-57 | 1.345849e-54 |
MsaG005210 | MsaG019452 | 0.811454 | 1.191350e-50 | 2.108054e-48 |
MsaG005259 | MsaG019452 | 0.809156 | 3.710828e-50 | 6.209126e-48 |
MsaG005329 | MsaG019452 | 0.812478 | 7.142992e-51 | 1.296266e-48 |
MsaG005510 | MsaG019452 | 0.803389 | 6.006680e-49 | 8.772822e-47 |
MsaG006623 | MsaG019452 | 0.808540 | 5.018084e-50 | 8.273047e-48 |
MsaG006683 | MsaG019452 | 0.807167 | 9.798308e-50 | 1.563047e-47 |
MsaG006988 | MsaG019452 | 0.837280 | 1.045766e-56 | 3.751640e-54 |
MsaG007000 | MsaG019452 | 0.828490 | 1.563288e-54 | 4.334347e-52 |
MsaG007215 | MsaG019452 | 0.801230 | 1.663605e-48 | 2.312549e-46 |
MsaG007501 | MsaG019452 | 0.816848 | 7.774775e-52 | 1.575301e-49 |
MsaG007568 | MsaG019452 | 0.823415 | 2.477677e-53 | 5.969403e-51 |
MsaG007828 | MsaG019452 | 0.800902 | 1.940222e-48 | 2.677194e-46 |
MsaG007834 | MsaG019452 | 0.804095 | 4.294114e-49 | 6.375156e-47 |
MsaG008218 | MsaG019452 | 0.814676 | 2.359365e-51 | 4.523580e-49 |
MsaG008356 | MsaG019452 | 0.812231 | 8.082017e-51 | 1.457621e-48 |
MsaG009015 | MsaG019452 | 0.830107 | 6.358366e-55 | 1.845834e-52 |
MsaG009657 | MsaG019452 | 0.813047 | 5.370602e-51 | 9.885238e-49 |
MsaG009767 | MsaG019452 | 0.811391 | 1.229047e-50 | 2.171427e-48 |
MsaG010050 | MsaG019452 | 0.800300 | 2.570119e-48 | 3.498470e-46 |
MsaG010104 | MsaG019452 | 0.820169 | 1.384754e-52 | 3.059391e-50 |
MsaG010768 | MsaG019452 | 0.842440 | 4.804972e-58 | 2.026613e-55 |
MsaG011010 | MsaG019452 | 0.816513 | 9.236958e-52 | 1.855505e-49 |
MsaG011109 | MsaG019452 | 0.824905 | 1.110673e-53 | 2.786750e-51 |
MsaG012037 | MsaG019452 | 0.840231 | 1.820397e-57 | 7.157989e-55 |
MsaG012263 | MsaG019452 | 0.804173 | 4.135562e-49 | 6.150778e-47 |
MsaG012568 | MsaG019452 | 0.804540 | 3.471427e-49 | 5.207083e-47 |
MsaG014774 | MsaG019452 | 0.846381 | 4.237018e-59 | 2.032232e-56 |
MsaG015184 | MsaG019452 | 0.864600 | 2.158470e-64 | 2.006933e-61 |
MsaG017150 | MsaG019452 | 0.823135 | 2.877871e-53 | 6.881195e-51 |
MsaG017620 | MsaG019452 | 0.813535 | 4.201095e-51 | 7.827581e-49 |
MsaG017946 | MsaG019452 | 0.804840 | 3.007042e-49 | 4.542088e-47 |
MsaG018290 | MsaG019452 | 0.830239 | 5.907148e-55 | 1.721269e-52 |
MsaG018489 | MsaG019452 | 0.832307 | 1.841862e-55 | 5.697886e-53 |
MsaG018786 | MsaG019452 | 0.827211 | 3.164000e-54 | 8.462754e-52 |
MsaG018793 | MsaG019452 | 0.838678 | 4.589426e-57 | 1.719035e-54 |
MsaG018874 | MsaG019452 | 0.829489 | 8.978329e-55 | 2.560694e-52 |
MsaG019108 | MsaG019452 | 0.826661 | 4.276218e-54 | 1.126347e-51 |
MsaG019452 | MsaG019564 | 0.860570 | 3.708113e-63 | 2.946689e-60 |
MsaG019452 | MsaG019573 | 0.802420 | 9.506187e-49 | 1.357788e-46 |
MsaG019452 | MsaG019628 | 0.860349 | 4.322647e-63 | 3.406527e-60 |
MsaG019452 | MsaG019654 | 0.852201 | 1.032979e-60 | 6.042847e-58 |
MsaG019452 | MsaG019660 | 0.824745 | 1.211623e-53 | 3.026572e-51 |
MsaG019452 | MsaG019935 | 0.840654 | 1.412883e-57 | 5.629933e-55 |
MsaG019452 | MsaG019985 | 0.842518 | 4.582679e-58 | 1.937712e-55 |
MsaG019452 | MsaG020288 | 0.810185 | 2.234767e-50 | 3.833522e-48 |
MsaG019452 | MsaG020334 | 0.833439 | 9.668218e-56 | 3.091721e-53 |
MsaG019452 | MsaG020420 | 0.845006 | 9.959488e-59 | 4.565144e-56 |
MsaG019452 | MsaG020467 | 0.861551 | 1.871199e-63 | 1.543323e-60 |
MsaG019452 | MsaG021488 | 0.824497 | 1.385045e-53 | 3.436548e-51 |
MsaG019452 | MsaG021775 | 0.803090 | 6.921806e-49 | 1.003982e-46 |
MsaG019452 | MsaG022345 | 0.817959 | 4.384407e-52 | 9.141496e-50 |
MsaG019452 | MsaG022486 | 0.808404 | 5.361645e-50 | 8.810351e-48 |
MsaG019452 | MsaG022515 | 0.831859 | 2.374484e-55 | 7.251074e-53 |
MsaG019452 | MsaG022547 | 0.808402 | 5.368346e-50 | 8.820790e-48 |
MsaG019452 | MsaG023005 | 0.811294 | 1.289861e-50 | 2.273441e-48 |
MsaG019452 | MsaG023275 | 0.825420 | 8.403974e-54 | 2.138533e-51 |
MsaG019452 | MsaG023623 | 0.837747 | 7.952030e-57 | 2.893782e-54 |
MsaG019452 | MsaG024055 | 0.800093 | 2.830438e-48 | 3.834917e-46 |
MsaG019452 | MsaG024114 | 0.847649 | 1.911047e-59 | 9.563943e-57 |
MsaG019452 | MsaG024272 | 0.838141 | 6.301983e-57 | 2.321392e-54 |
MsaG019452 | MsaG026022 | 0.825468 | 8.191177e-54 | 2.087141e-51 |
MsaG019452 | MsaG026478 | 0.830503 | 5.093884e-55 | 1.495473e-52 |
MsaG019452 | MsaG026841 | 0.830360 | 5.519107e-55 | 1.613786e-52 |
MsaG019452 | MsaG026849 | 0.837987 | 6.903352e-57 | 2.530794e-54 |
MsaG019452 | MsaG026830 | 0.813167 | 5.054512e-51 | 9.331469e-49 |
MsaG019452 | MsaG026831 | 0.823940 | 1.868759e-53 | 4.566952e-51 |
MsaG019452 | MsaG027026 | 0.836394 | 1.755622e-56 | 6.132307e-54 |
MsaG019452 | MsaG026987 | 0.802334 | 9.898062e-49 | 1.411002e-46 |
MsaG019452 | MsaG027055 | 0.815116 | 1.885938e-51 | 3.656278e-49 |
MsaG019452 | MsaG027303 | 0.821007 | 8.912990e-53 | 2.013275e-50 |
MsaG019452 | MsaG027344 | 0.831170 | 3.503104e-55 | 1.048365e-52 |
MsaG019452 | MsaG027775 | 0.826441 | 4.821766e-54 | 1.262387e-51 |
MsaG019452 | MsaG027992 | 0.808696 | 4.649521e-50 | 7.694501e-48 |
MsaG019452 | MsaG028109 | 0.833984 | 7.074462e-56 | 2.298753e-53 |
MsaG019452 | MsaG028163 | 0.830411 | 5.363360e-55 | 1.570565e-52 |
MsaG019452 | MsaG029413 | 0.833236 | 1.085759e-55 | 3.451509e-53 |
MsaG019452 | MsaG029473 | 0.812063 | 8.791669e-51 | 1.579034e-48 |
MsaG019452 | MsaG030045 | 0.831311 | 3.235592e-55 | 9.722615e-53 |
MsaG019452 | MsaG030070 | 0.828624 | 1.451414e-54 | 4.039264e-52 |
MsaG019452 | MsaG030359 | 0.801863 | 1.236041e-48 | 1.743134e-46 |
MsaG019452 | MsaG030546 | 0.834971 | 4.010349e-56 | 1.342108e-53 |
MsaG019452 | MsaG031622 | 0.819361 | 2.114154e-52 | 4.572231e-50 |
MsaG019452 | MsaG032046 | 0.800973 | 1.876317e-48 | 2.593194e-46 |
MsaG019452 | MsaG032064 | 0.819034 | 2.507443e-52 | 5.376399e-50 |
MsaG019452 | MsaG032272 | 0.872577 | 5.866281e-67 | 7.587670e-64 |
MsaG019452 | MsaG032221 | 0.800802 | 2.032830e-48 | 2.798786e-46 |
MsaG019452 | MsaG032422 | 0.808562 | 4.965046e-50 | 8.189832e-48 |
MsaG019452 | MsaG033593 | 0.812654 | 6.539559e-51 | 1.191921e-48 |
MsaG019452 | MsaG034946 | 0.804798 | 3.068379e-49 | 4.630033e-47 |
MsaG019452 | MsaG034996 | 0.819274 | 2.212251e-52 | 4.773409e-50 |
MsaG019452 | MsaG035529 | 0.802161 | 1.073694e-48 | 1.524475e-46 |
MsaG019452 | MsaG036153 | 0.813958 | 3.393816e-51 | 6.390942e-49 |
MsaG019452 | MsaG040425 | 0.835359 | 3.203784e-56 | 1.084731e-53 |
MsaG019452 | MsaG041112 | 0.819788 | 1.691341e-52 | 3.698870e-50 |
MsaG019452 | MsaG041336 | 0.802466 | 9.301972e-49 | 1.329981e-46 |
MsaG019452 | MsaG042054 | 0.818737 | 2.927873e-52 | 6.228717e-50 |
MsaG019452 | MsaG042103 | 0.826838 | 3.880294e-54 | 1.027121e-51 |
MsaG019452 | MsaG042579 | 0.822708 | 3.614297e-53 | 8.543049e-51 |
MsaG019452 | MsaG042679 | 0.801736 | 1.311813e-48 | 1.844659e-46 |
MsaG019452 | MsaG042717 | 0.871606 | 1.229660e-66 | 1.525649e-63 |
MsaG019452 | MsaG044278 | 0.821733 | 6.069841e-53 | 1.397667e-50 |
MsaG019452 | MsaG044584 | 0.829865 | 7.281870e-55 | 2.099317e-52 |
MsaG019452 | MsaG044934 | 0.817236 | 6.369969e-52 | 1.303678e-49 |
MsaG019452 | MsaG045238 | 0.830957 | 3.948819e-55 | 1.174534e-52 |
MsaG019452 | MsaG045371 | 0.817056 | 6.987655e-52 | 1.423485e-49 |
MsaG019452 | MsaG045568 | 0.844633 | 1.254130e-58 | 5.678169e-56 |
MsaG019452 | MsaG045767 | 0.838710 | 4.503446e-57 | 1.688506e-54 |
MsaG019452 | MsaG045769 | 0.872338 | 7.041960e-67 | 9.016290e-64 |
MsaG019452 | MsaG046472 | 0.804382 | 3.743861e-49 | 5.595199e-47 |
MsaG019452 | MsaG046473 | 0.829014 | 1.169392e-54 | 3.290103e-52 |
MsaG019452 | MsaG046498 | 0.823926 | 1.883552e-53 | 4.601229e-51 |
MsaG019452 | MsaG046507 | 0.840738 | 1.343143e-57 | 5.366136e-55 |
MsaG019452 | MsaG047047 | 0.800542 | 2.296184e-48 | 3.142789e-46 |
MsaG019452 | MsaG001564 | 0.863160 | 6.023132e-64 | 5.289919e-61 |
MsaG019452 | MsaG002242 | 0.815207 | 1.800851e-51 | 3.499518e-49 |
MsaG019452 | MsaG001950 | 0.827054 | 3.447448e-54 | 9.180530e-52 |
MsaG019452 | MsaG003742 | 0.827293 | 3.023965e-54 | 8.107076e-52 |
MsaG019452 | MsaG004474 | 0.810688 | 1.742648e-50 | 3.026223e-48 |
MsaG019452 | MsaG013712 | 0.801688 | 1.342108e-48 | 1.885166e-46 |
MsaG019452 | MsaG013706 | 0.807431 | 8.617336e-50 | 1.383330e-47 |
MsaG019452 | MsaG013252 | 0.829779 | 7.638179e-55 | 2.196596e-52 |
MsaG019452 | MsaG018678 | 0.801465 | 1.490074e-48 | 2.082445e-46 |
MsaG019452 | MsaG017935 | 0.827026 | 3.500972e-54 | 9.315611e-52 |
MsaG019452 | MsaG019592 | 0.806350 | 1.454763e-49 | 2.276447e-47 |
MsaG019452 | MsaG028121 | 0.815827 | 1.312873e-51 | 2.591567e-49 |
MsaG019452 | MsaG027608 | 0.855100 | 1.530074e-61 | 9.920031e-59 |
MsaG019452 | MsaG028944 | 0.801884 | 1.223471e-48 | 1.726272e-46 |
MsaG019452 | MsaG033233 | 0.834016 | 6.946018e-56 | 2.259122e-53 |
MsaG019452 | MsaG033253 | 0.814474 | 2.613691e-51 | 4.985884e-49 |
MsaG019452 | MsaG030007 | 0.803353 | 6.109550e-49 | 8.915696e-47 |
MsaG019452 | MsaG034262 | 0.818413 | 3.463414e-52 | 7.306395e-50 |
MsaG019452 | MsaG033504 | 0.819000 | 2.552965e-52 | 5.468880e-50 |
MsaG019452 | MsaG036873 | 0.802228 | 1.040583e-48 | 1.479727e-46 |
MsaG019452 | MsaG036787 | 0.806813 | 1.163205e-49 | 1.840107e-47 |
MsaG019452 | MsaG035914 | 0.804117 | 4.247909e-49 | 6.309694e-47 |
MsaG019452 | MsaG035795 | 0.836694 | 1.473988e-56 | 5.194871e-54 |
MsaG019452 | MsaG042698 | 0.822931 | 3.210014e-53 | 7.633120e-51 |
MsaG019452 | MsaG042291 | 0.832542 | 1.611755e-55 | 5.020184e-53 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG019452 | MtrunA17_Chr4g0014921 | 96.651 | 209 | 7 | 0 | 2 | 210 | 72 | 280 | 3.59e-152 | 423 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG019452 | AT1G72030.1 | 54.040 | 198 | 85 | 3 | 14 | 206 | 60 | 256 | 1.98e-62 | 195 |
Find 36 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
AATTAATATTCTACTTAATT+AGG | 0.161235 | 4:-28932549 | None:intergenic |
CAAGAGTACCTTTACATTTC+AGG | 0.191164 | 4:+28931843 | MsaT019452.1:CDS |
TAGCTGTCTCCAATGCTTTC+AGG | 0.230533 | 4:+28931868 | MsaT019452.1:CDS |
ATCCACCATGGACAAGTAAT+TGG | 0.234201 | 4:+28932478 | MsaT019452.1:CDS |
TTTGTTCTTTCAATTCTTTA+AGG | 0.237732 | 4:+28930960 | MsaT019452.1:CDS |
AAGAGCTTATGAAGAAGATT+TGG | 0.266902 | 4:+28932407 | MsaT019452.1:CDS |
AATCTTCTTCATAAGCTCTT+AGG | 0.289976 | 4:-28932404 | None:intergenic |
AAGAAAATGTCGTGTTCTTA+TGG | 0.298968 | 4:+28932506 | MsaT019452.1:CDS |
AAATAGGCTGAAGTGCTTTC+AGG | 0.310898 | 4:+28931488 | MsaT019452.1:intron |
AAACTCATTACCCCACAAAT+TGG | 0.313360 | 4:-28932376 | None:intergenic |
ATTATATAGGTATGCTTGTT+TGG | 0.327298 | 4:+28931719 | MsaT019452.1:intron |
TGAAAATATGAAGCAAAAGT+TGG | 0.383459 | 4:+28930816 | MsaT019452.1:CDS |
TAACAACAAATGTACCTATC+AGG | 0.398497 | 4:-28931539 | None:intergenic |
AGAGCTTATGAAGAAGATTT+GGG | 0.419407 | 4:+28932408 | MsaT019452.1:CDS |
GTGAATATGGATGGAGGATA+AGG | 0.442867 | 4:+28930851 | MsaT019452.1:CDS |
AGGTACTCTTGTGCATCAGC+AGG | 0.454568 | 4:-28931831 | None:intergenic |
GATTCATCAAAGGAACTTGT+AGG | 0.457006 | 4:+28931759 | MsaT019452.1:CDS |
TTAAATACTCACCTCCTGAT+AGG | 0.463612 | 4:+28931525 | MsaT019452.1:CDS |
GTGACATAATATCCAATTTG+TGG | 0.473968 | 4:+28932364 | MsaT019452.1:CDS |
TCCACCATGGACAAGTAATT+GGG | 0.475166 | 4:+28932479 | MsaT019452.1:CDS |
TGACATAATATCCAATTTGT+GGG | 0.486196 | 4:+28932365 | MsaT019452.1:CDS |
AAATCATTGAAAAGAGCTAC+AGG | 0.509660 | 4:-28930940 | None:intergenic |
CGAACATTGTACACAAATGC+TGG | 0.511040 | 4:+28932435 | MsaT019452.1:CDS |
CAGCTATTCCTGAAATGTAA+AGG | 0.520945 | 4:-28931851 | None:intergenic |
GGAGATGTTCAAGCACATCT+TGG | 0.525750 | 4:-28931809 | None:intergenic |
ATATAGGTATGCTTGTTTGG+TGG | 0.525798 | 4:+28931722 | MsaT019452.1:intron |
CCAACCCAATTACTTGTCCA+TGG | 0.539516 | 4:-28932483 | None:intergenic |
CCATGGACAAGTAATTGGGT+TGG | 0.581303 | 4:+28932483 | MsaT019452.1:CDS |
GTGGATCTTTAGACACAAGT+TGG | 0.596499 | 4:-28932461 | None:intergenic |
TGGTGTGTGAATATGGATGG+AGG | 0.600815 | 4:+28930845 | MsaT019452.1:CDS |
GGTACTCTTGTGCATCAGCA+GGG | 0.603876 | 4:-28931830 | None:intergenic |
GAATGATCAAGATTCATCAA+AGG | 0.633438 | 4:+28931749 | MsaT019452.1:CDS |
ACCCAATTACTTGTCCATGG+TGG | 0.661718 | 4:-28932480 | None:intergenic |
CAACAAATGTACCTATCAGG+AGG | 0.675327 | 4:-28931536 | None:intergenic |
GACATAATATCCAATTTGTG+GGG | 0.680586 | 4:+28932366 | MsaT019452.1:CDS |
TTGTGTCTAAAGATCCACCA+TGG | 0.708310 | 4:+28932466 | MsaT019452.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr4 | gene | 28930793 | 28932557 | 28930793 | ID=MsaG019452 |
Chr4 | mRNA | 28930793 | 28932557 | 28930793 | ID=MsaT019452.1;Parent=MsaG019452 |
Chr4 | exon | 28930793 | 28930981 | 28930793 | ID=MsaT019452.1.exon1;Parent=MsaT019452.1 |
Chr4 | CDS | 28930793 | 28930981 | 28930793 | ID=cds.MsaT019452.1;Parent=MsaT019452.1 |
Chr4 | exon | 28931494 | 28931546 | 28931494 | ID=MsaT019452.1.exon2;Parent=MsaT019452.1 |
Chr4 | CDS | 28931494 | 28931546 | 28931494 | ID=cds.MsaT019452.1;Parent=MsaT019452.1 |
Chr4 | exon | 28931728 | 28931889 | 28931728 | ID=MsaT019452.1.exon3;Parent=MsaT019452.1 |
Chr4 | CDS | 28931728 | 28931889 | 28931728 | ID=cds.MsaT019452.1;Parent=MsaT019452.1 |
Chr4 | exon | 28932329 | 28932557 | 28932329 | ID=MsaT019452.1.exon4;Parent=MsaT019452.1 |
Chr4 | CDS | 28932329 | 28932557 | 28932329 | ID=cds.MsaT019452.1;Parent=MsaT019452.1 |
Gene Sequence |
Protein sequence |