AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019452


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019452 MtrunA17_Chr4g0014921 96.651 209 7 0 2 210 72 280 3.59e-152 423
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019452 AT1G72030.1 54.040 198 85 3 14 206 60 256 1.98e-62 195

Find 36 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAATATTCTACTTAATT+AGG 0.161235 4:-28932549 None:intergenic
CAAGAGTACCTTTACATTTC+AGG 0.191164 4:+28931843 MsaT019452.1:CDS
TAGCTGTCTCCAATGCTTTC+AGG 0.230533 4:+28931868 MsaT019452.1:CDS
ATCCACCATGGACAAGTAAT+TGG 0.234201 4:+28932478 MsaT019452.1:CDS
TTTGTTCTTTCAATTCTTTA+AGG 0.237732 4:+28930960 MsaT019452.1:CDS
AAGAGCTTATGAAGAAGATT+TGG 0.266902 4:+28932407 MsaT019452.1:CDS
AATCTTCTTCATAAGCTCTT+AGG 0.289976 4:-28932404 None:intergenic
AAGAAAATGTCGTGTTCTTA+TGG 0.298968 4:+28932506 MsaT019452.1:CDS
AAATAGGCTGAAGTGCTTTC+AGG 0.310898 4:+28931488 MsaT019452.1:intron
AAACTCATTACCCCACAAAT+TGG 0.313360 4:-28932376 None:intergenic
ATTATATAGGTATGCTTGTT+TGG 0.327298 4:+28931719 MsaT019452.1:intron
TGAAAATATGAAGCAAAAGT+TGG 0.383459 4:+28930816 MsaT019452.1:CDS
TAACAACAAATGTACCTATC+AGG 0.398497 4:-28931539 None:intergenic
AGAGCTTATGAAGAAGATTT+GGG 0.419407 4:+28932408 MsaT019452.1:CDS
GTGAATATGGATGGAGGATA+AGG 0.442867 4:+28930851 MsaT019452.1:CDS
AGGTACTCTTGTGCATCAGC+AGG 0.454568 4:-28931831 None:intergenic
GATTCATCAAAGGAACTTGT+AGG 0.457006 4:+28931759 MsaT019452.1:CDS
TTAAATACTCACCTCCTGAT+AGG 0.463612 4:+28931525 MsaT019452.1:CDS
GTGACATAATATCCAATTTG+TGG 0.473968 4:+28932364 MsaT019452.1:CDS
TCCACCATGGACAAGTAATT+GGG 0.475166 4:+28932479 MsaT019452.1:CDS
TGACATAATATCCAATTTGT+GGG 0.486196 4:+28932365 MsaT019452.1:CDS
AAATCATTGAAAAGAGCTAC+AGG 0.509660 4:-28930940 None:intergenic
CGAACATTGTACACAAATGC+TGG 0.511040 4:+28932435 MsaT019452.1:CDS
CAGCTATTCCTGAAATGTAA+AGG 0.520945 4:-28931851 None:intergenic
GGAGATGTTCAAGCACATCT+TGG 0.525750 4:-28931809 None:intergenic
ATATAGGTATGCTTGTTTGG+TGG 0.525798 4:+28931722 MsaT019452.1:intron
CCAACCCAATTACTTGTCCA+TGG 0.539516 4:-28932483 None:intergenic
CCATGGACAAGTAATTGGGT+TGG 0.581303 4:+28932483 MsaT019452.1:CDS
GTGGATCTTTAGACACAAGT+TGG 0.596499 4:-28932461 None:intergenic
TGGTGTGTGAATATGGATGG+AGG 0.600815 4:+28930845 MsaT019452.1:CDS
GGTACTCTTGTGCATCAGCA+GGG 0.603876 4:-28931830 None:intergenic
GAATGATCAAGATTCATCAA+AGG 0.633438 4:+28931749 MsaT019452.1:CDS
ACCCAATTACTTGTCCATGG+TGG 0.661718 4:-28932480 None:intergenic
CAACAAATGTACCTATCAGG+AGG 0.675327 4:-28931536 None:intergenic
GACATAATATCCAATTTGTG+GGG 0.680586 4:+28932366 MsaT019452.1:CDS
TTGTGTCTAAAGATCCACCA+TGG 0.708310 4:+28932466 MsaT019452.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 28930793 28932557 28930793 ID=MsaG019452
Chr4 mRNA 28930793 28932557 28930793 ID=MsaT019452.1;Parent=MsaG019452
Chr4 exon 28930793 28930981 28930793 ID=MsaT019452.1.exon1;Parent=MsaT019452.1
Chr4 CDS 28930793 28930981 28930793 ID=cds.MsaT019452.1;Parent=MsaT019452.1
Chr4 exon 28931494 28931546 28931494 ID=MsaT019452.1.exon2;Parent=MsaT019452.1
Chr4 CDS 28931494 28931546 28931494 ID=cds.MsaT019452.1;Parent=MsaT019452.1
Chr4 exon 28931728 28931889 28931728 ID=MsaT019452.1.exon3;Parent=MsaT019452.1
Chr4 CDS 28931728 28931889 28931728 ID=cds.MsaT019452.1;Parent=MsaT019452.1
Chr4 exon 28932329 28932557 28932329 ID=MsaT019452.1.exon4;Parent=MsaT019452.1
Chr4 CDS 28932329 28932557 28932329 ID=cds.MsaT019452.1;Parent=MsaT019452.1
Gene Sequence

>MsaT019452.1

ATGTTGGAAGTTGAGAATGATAGTGAAAATATGAAGCAAAAGTTGGATTTTTTGGTGTGTGAATATGGATGGAGGATAAGGAGATTAATTGAGAATGCAGATGAGATAAAAATGGCTGCTCAAGTTCAAGCTGAAGCTTTTCATGATCCTGTAGCTCTTTTCAATGATTTGTTCTTTCAATTCTTTAAGGCTGAAGTGCTTTCAGGACTGCTTTACAAGCTTAAATACTCACCTCCTGATAGGTATGCTTGTTTGGTGGCTGAGAATGATCAAGATTCATCAAAGGAACTTGTAGGTGTTATTGATGTTACTGTAATGAGAGACCAAGATGTGCTTGAACATCTCCCTGCTGATGCACAAGAGTACCTTTACATTTCAGGAATAGCTGTCTCCAATGCTTTCAGGAGGAGGAAAATTGCAACTGTTATGTTAAAAGCATGTGACATAATATCCAATTTGTGGGGTAATGAGTTTCTTGTCCTAAGAGCTTATGAAGAAGATTTGGGTGCTCGAACATTGTACACAAATGCTGGCTACCAACTTGTGTCTAAAGATCCACCATGGACAAGTAATTGGGTTGGAAGAAAATGTCGTGTTCTTATGGTCAAAAGAATTAGTTTATTGCCTAATTAA

Protein sequence

>MsaT019452.1

MLEVENDSENMKQKLDFLVCEYGWRIRRLIENADEIKMAAQVQAEAFHDPVALFNDLFFQFFKAEVLSGLLYKLKYSPPDRYACLVAENDQDSSKELVGVIDVTVMRDQDVLEHLPADAQEYLYISGIAVSNAFRRRKIATVMLKACDIISNLWGNEFLVLRAYEEDLGARTLYTNAGYQLVSKDPPWTSNWVGRKCRVLMVKRISLLPN*