AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019527


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019527 MtrunA17_Chr4g0018761 67.347 245 74 1 1 239 1 245 2.16e-117 335
MsaG019527 MtrunA17_Chr4g0018741 80.769 104 20 0 78 181 1 104 1.43e-56 176
MsaG019527 MtrunA17_Chr3g0079061 26.038 265 158 6 3 229 2 266 1.72e-16 76.6
MsaG019527 MtrunA17_Chr3g0079131 25.806 279 139 7 7 232 6 269 1.86e-16 76.6
MsaG019527 MtrunA17_Chr4g0056091 59.322 59 22 1 173 229 210 268 4.77e-14 70.5
MsaG019527 MtrunA17_Chr7g0231181 41.975 81 37 1 170 240 239 319 6.68e-14 70.1
MsaG019527 MtrunA17_Chr3g0079091 35.484 124 74 3 3 121 2 124 1.69e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019527 AT3G60580.1 30.566 265 116 6 9 229 6 246 6.11e-19 84.0
MsaG019527 AT1G02030.1 45.570 79 30 2 171 236 160 238 1.84e-13 68.6
MsaG019527 AT5G61470.1 43.836 73 29 2 168 229 225 296 1.24e-12 66.6

Find 64 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAACACTTTGTTGCAATT+TGG 0.166386 4:+30164657 None:intergenic
TTCTGTGTTTGTTGTATTTC+GGG 0.187551 4:+30165086 None:intergenic
ATTCTGTGTTTGTTGTATTT+CGG 0.237824 4:+30165085 None:intergenic
AATTGGATCTCTTGCGGGTT+GGG 0.313326 4:+30164982 None:intergenic
TGAACACTTTGTTGCAATTT+GGG 0.315013 4:+30164658 None:intergenic
TGAGTATTAGTTTCAAGCTT+TGG 0.337317 4:+30165176 None:intergenic
GCCGCAATTGGATCTCTTGC+GGG 0.342035 4:+30164977 None:intergenic
GTACACTCTTCCAGTGCTAA+TGG 0.389630 4:-30164603 MsaT019527.1:CDS
CAATTGGATCTCTTGCGGGT+TGG 0.402481 4:+30164981 None:intergenic
TACTCAAGCTGTAGACAATT+CGG 0.436960 4:-30165159 MsaT019527.1:CDS
ATTAAGTTCGCGGCCGCAAT+TGG 0.437633 4:+30164965 None:intergenic
ATTTGTGGGAAGTGGTTCTC+CGG 0.453707 4:-30165254 MsaT019527.1:CDS
GTTTCAAGCTTTGGAGCAAT+CGG 0.454553 4:+30165185 None:intergenic
TTCCGATCTTATCAGGCTCT+TGG 0.457389 4:-30164747 MsaT019527.1:CDS
TTCTCCGGTGGGAAAGCCCT+TGG 0.463478 4:-30165239 MsaT019527.1:CDS
TTGCAATTTGGGCAGTGAAA+AGG 0.463868 4:+30164669 None:intergenic
GGAAAGCCTGTGGGATTTGT+GGG 0.467351 4:-30165268 MsaT019527.1:CDS
GGGAAAGCCTGTGGGATTTG+TGG 0.468727 4:-30165269 MsaT019527.1:CDS
CGATCTTATCAGGCTCTTGG+TGG 0.468794 4:-30164744 MsaT019527.1:CDS
GCCTCCAAGGGCTTTCCCAC+CGG 0.472807 4:+30165235 None:intergenic
AGATCTCATGTGGCCTCCAA+GGG 0.485485 4:+30165223 None:intergenic
TACTGCAAAAGAGGCAATGA+TGG 0.488382 4:-30164711 MsaT019527.1:CDS
TCCGGTGGGAAAGCCCTTGG+AGG 0.494800 4:-30165236 MsaT019527.1:CDS
TTCAAGTCTGCTCAGGCACT+CGG 0.500253 4:-30164639 MsaT019527.1:CDS
CCCCATAACATCAAAACACC+TGG 0.500612 4:+30164909 None:intergenic
GGCCGCAATTGGATCTCTTG+CGG 0.503301 4:+30164976 None:intergenic
TCTGTTTGAGTTTGCAGAGG+AGG 0.504442 4:+30165034 None:intergenic
AACGATGGCGAAAGCAGTAC+AGG 0.507873 4:-30164831 MsaT019527.1:CDS
AGCAAATTGATTTCGACGAT+GGG 0.509489 4:+30165127 None:intergenic
GCACCTCTCACAGTTGAACC+TGG 0.509618 4:+30164779 None:intergenic
ATTTCGACGATGGGTTGAAT+CGG 0.510947 4:+30165136 None:intergenic
CAGCCGCAGCATTAAGTTCG+CGG 0.512384 4:+30164955 None:intergenic
TTCTCTGTTTGAGTTTGCAG+AGG 0.512537 4:+30165031 None:intergenic
GAGATCTCATGTGGCCTCCA+AGG 0.514030 4:+30165222 None:intergenic
GAAGATATGTCAAGAGATCC+AGG 0.516117 4:-30164927 MsaT019527.1:CDS
TGTGGGAAGTGGTTCTCCGG+TGG 0.517822 4:-30165251 MsaT019527.1:CDS
CAAAGTGTTCAAGTCTGCTC+AGG 0.524975 4:-30164646 MsaT019527.1:CDS
CACAGAGTATACTGCAAAAG+AGG 0.526500 4:-30164720 MsaT019527.1:CDS
CACAAGCAGACTCTGAAGCC+AGG 0.539423 4:-30164797 MsaT019527.1:CDS
GGGGACTTGAAAATTCAATA+CGG 0.545028 4:+30164561 None:intergenic
ACTTTGAAATCCATTAGCAC+TGG 0.550464 4:+30164593 None:intergenic
AAGCAAATTGATTTCGACGA+TGG 0.551421 4:+30165126 None:intergenic
GTGTTTGTTGTATTTCGGGA+AGG 0.552910 4:+30165090 None:intergenic
CCTGTGGGATTTGTGGGAAG+TGG 0.558125 4:-30165262 MsaT019527.1:CDS
GGGACTTGAAAATTCAATAC+GGG 0.558578 4:+30164562 None:intergenic
GTGGGAAGTGGTTCTCCGGT+GGG 0.561353 4:-30165250 MsaT019527.1:CDS
TTGAAAATTCAATACGGGTG+TGG 0.569607 4:+30164567 None:intergenic
GATCTTATCAGGCTCTTGGT+GGG 0.571526 4:-30164743 MsaT019527.1:CDS
GCAACTATGGAGAAGAGCGA+TGG 0.579019 4:-30165290 None:intergenic
CACCAAGAGCCTGATAAGAT+CGG 0.582662 4:+30164745 None:intergenic
AAGAGCGATGGGAAAGCCTG+TGG 0.598960 4:-30165278 MsaT019527.1:CDS
AGGCACTCGGTGGACACAAG+AGG 0.606213 4:-30164626 MsaT019527.1:CDS
CAACTATGGAGAAGAGCGAT+GGG 0.616240 4:-30165289 None:intergenic
AAGCCAGGTTCAACTGTGAG+AGG 0.622627 4:-30164782 MsaT019527.1:CDS
GGCACTCGGTGGACACAAGA+GGG 0.632635 4:-30164625 MsaT019527.1:CDS
CCACTTCCCACAAATCCCAC+AGG 0.647858 4:+30165262 None:intergenic
AGAGCGATGGGAAAGCCTGT+GGG 0.654994 4:-30165277 MsaT019527.1:CDS
CGGCCGCGAACTTAATGCTG+CGG 0.660556 4:-30164958 MsaT019527.1:CDS
AGGTTCAACTGTGAGAGGTG+CGG 0.663022 4:-30164777 MsaT019527.1:CDS
TTCGGGAAGGCAAGTCTGTG+AGG 0.669270 4:+30165103 None:intergenic
AAGTCTGCTCAGGCACTCGG+TGG 0.670340 4:-30164636 MsaT019527.1:CDS
AGGCCACATGAGATCTCACA+TGG 0.682156 4:-30165216 MsaT019527.1:CDS
ACCCGCAAGAGATCCAATTG+CGG 0.686880 4:-30164978 MsaT019527.1:CDS
GAACCATGTGAGATCTCATG+TGG 0.730676 4:+30165213 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 30164575 30165306 30164575 ID=MsaG019527
Chr4 mRNA 30164575 30165306 30164575 ID=MsaT019527.1;Parent=MsaG019527
Chr4 exon 30164575 30165306 30164575 ID=MsaT019527.1.exon1;Parent=MsaT019527.1
Chr4 CDS 30164575 30165306 30164575 ID=cds.MsaT019527.1;Parent=MsaT019527.1
Gene Sequence

>MsaT019527.1

ATGGAGAAGAGCGATGGGAAAGCCTGTGGGATTTGTGGGAAGTGGTTCTCCGGTGGGAAAGCCCTTGGAGGCCACATGAGATCTCACATGGTTCATTTGCCGATTGCTCCAAAGCTTGAAACTAATACTCAAGCTGTAGACAATTCGGCCGATTCAACCCATCGTCGAAATCAATTTGCTTCCTCACAGACTTGCCTTCCCGAAATACAACAAACACAGAATTTCCTATCCATGAAACAAAACTTCGCTGCCTCCTCTGCAAACTCAAACAGAGAAAATGAATCTGTGTCCAACCTGAAAAACCCAACCCGCAAGAGATCCAATTGCGGCCGCGAACTTAATGCTGCGGCTGATACAGAAGATATGTCAAGAGATCCAGGTGTTTTGATGTTATGGGGATTTTACAAAGATGCGCAAAAAAGGGAAGGGATCAAGAAACAAAAAATGAGGGAAAACGATGGCGAAAGCAGTACAGGTGACTCGCTTGCACAAGCAGACTCTGAAGCCAGGTTCAACTGTGAGAGGTGCGGAAAAATGTTCCGATCTTATCAGGCTCTTGGTGGGCACAGAGTATACTGCAAAAGAGGCAATGATGGTGATTCAATAGATCAGAAACCTTTTCACTGCCCAAATTGCAACAAAGTGTTCAAGTCTGCTCAGGCACTCGGTGGACACAAGAGGGTACACTCTTCCAGTGCTAATGGATTTCAAAGTGTGCCACACCCGTATTGA

Protein sequence

>MsaT019527.1

MEKSDGKACGICGKWFSGGKALGGHMRSHMVHLPIAPKLETNTQAVDNSADSTHRRNQFASSQTCLPEIQQTQNFLSMKQNFAASSANSNRENESVSNLKNPTRKRSNCGRELNAAADTEDMSRDPGVLMLWGFYKDAQKREGIKKQKMRENDGESSTGDSLAQADSEARFNCERCGKMFRSYQALGGHRVYCKRGNDGDSIDQKPFHCPNCNKVFKSAQALGGHKRVHSSSANGFQSVPHPY*