Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019566 | AES87959.1 | 78.632 | 117 | 25 | 0 | 1 | 117 | 1 | 117 | 8.67e-54 | 174 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019566 | tr|G7JTJ2|G7JTJ2_MEDTR | 78.632 | 117 | 25 | 0 | 1 | 117 | 1 | 117 | 4.14e-54 | 174 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG019566 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019566 | MtrunA17_Chr4g0019481 | 78.632 | 117 | 25 | 0 | 1 | 117 | 1 | 117 | 7.98e-58 | 174 |
| MsaG019566 | MtrunA17_Chr4g0019471 | 76.336 | 131 | 17 | 2 | 1 | 117 | 1 | 131 | 6.43e-56 | 170 |
| MsaG019566 | MtrunA17_Chr4g0019551 | 72.650 | 117 | 32 | 0 | 1 | 117 | 1 | 117 | 1.44e-45 | 143 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCACTCATCTGGTGTATA+AGG | 0.223072 | 4:+31220029 | None:intergenic |
| TGTGGTTGAGGCTGGTTATT+TGG | 0.223810 | 4:+31220233 | None:intergenic |
| CTCGATTCTAATATATAATC+TGG | 0.322005 | 4:+31220272 | None:intergenic |
| GTGGTGGAGCCGGTGCATTT+TGG | 0.359073 | 4:+31220129 | None:intergenic |
| AGGTCAAAATCACGAACATT+AGG | 0.392346 | 4:+31220059 | None:intergenic |
| CTCACTCATCTGGTGTATAA+GGG | 0.433158 | 4:+31220030 | None:intergenic |
| TGAGGCTGGTTATTTGGTGG+TGG | 0.452210 | 4:+31220239 | None:intergenic |
| GGAGGCTGGTTTAAGTCAAA+TGG | 0.457633 | 4:+31220080 | None:intergenic |
| GGTTGAGGCTGGTTATTTGG+TGG | 0.492897 | 4:+31220236 | None:intergenic |
| CTGGTGTATAAGGGAGATTA+AGG | 0.499975 | 4:+31220039 | None:intergenic |
| AATCACGAACATTAGGAGGC+TGG | 0.541825 | 4:+31220066 | None:intergenic |
| ACTGCTAAAGCTTGTGGAGA+TGG | 0.545041 | 4:+31220317 | None:intergenic |
| AAGTCAAATGGCTGCACATG+TGG | 0.555104 | 4:+31220092 | None:intergenic |
| AATGGCTGCACATGTGGAGG+TGG | 0.555990 | 4:+31220098 | None:intergenic |
| ACATGCACTGCTAAAGCTTG+TGG | 0.565788 | 4:+31220311 | None:intergenic |
| TCAAAATCACGAACATTAGG+AGG | 0.598365 | 4:+31220062 | None:intergenic |
| ACATCAACACCAAAATGCAC+CGG | 0.630120 | 4:-31220138 | MsaT019566.1:CDS |
| ATATTAGAATCGAGTAAACA+AGG | 0.666840 | 4:-31220263 | MsaT019566.1:CDS |
| TCAAATGGCTGCACATGTGG+AGG | 0.676298 | 4:+31220095 | None:intergenic |
| TCAAAGTTAGAATTCATATG+CGG | 0.712015 | 4:-31220359 | MsaT019566.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 31220031 | 31220384 | 31220031 | ID=MsaG019566 |
| Chr4 | mRNA | 31220031 | 31220384 | 31220031 | ID=MsaT019566.1;Parent=MsaG019566 |
| Chr4 | exon | 31220031 | 31220384 | 31220031 | ID=MsaT019566.1.exon1;Parent=MsaT019566.1 |
| Chr4 | CDS | 31220031 | 31220384 | 31220031 | ID=cds.MsaT019566.1;Parent=MsaT019566.1 |
| Gene Sequence |
| Protein sequence |
