Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019592 | AES87959.1 | 82.906 | 117 | 19 | 1 | 1 | 116 | 1 | 117 | 2.66e-56 | 180 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019592 | tr|G7JTJ2|G7JTJ2_MEDTR | 82.906 | 117 | 19 | 1 | 1 | 116 | 1 | 117 | 1.27e-56 | 180 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000024 | MsaG019592 | 0.857927 | 2.281643e-62 | 1.640775e-59 |
| MsaG000045 | MsaG019592 | 0.846470 | 4.006703e-59 | 1.927411e-56 |
| MsaG000107 | MsaG019592 | 0.813961 | 3.388229e-51 | 6.380935e-49 |
| MsaG000117 | MsaG019592 | 0.841670 | 7.663817e-58 | 3.153539e-55 |
| MsaG000128 | MsaG019592 | 0.829104 | 1.111946e-54 | 3.136530e-52 |
| MsaG000179 | MsaG019592 | 0.801363 | 1.563083e-48 | 2.179430e-46 |
| MsaG000180 | MsaG019592 | 0.849345 | 6.519609e-60 | 3.455465e-57 |
| MsaG000207 | MsaG019592 | 0.868468 | 1.290799e-65 | 1.403711e-62 |
| MsaG000236 | MsaG019592 | 0.809770 | 2.742835e-50 | 4.658076e-48 |
| MsaG000237 | MsaG019592 | 0.854324 | 2.560630e-61 | 1.614976e-58 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019592 | MtrunA17_Chr4g0019481 | 82.906 | 117 | 19 | 1 | 1 | 116 | 1 | 117 | 2.45e-60 | 180 |
| MsaG019592 | MtrunA17_Chr4g0019471 | 74.046 | 131 | 19 | 2 | 1 | 116 | 1 | 131 | 8.49e-56 | 169 |
| MsaG019592 | MtrunA17_Chr4g0019551 | 76.923 | 117 | 26 | 1 | 1 | 116 | 1 | 117 | 1.11e-46 | 146 |
Find 17 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTCAAATGGTTGCACATG+TGG | 0.522527 | 4:-31927951 | None:intergenic |
| AATGGTTGCACATGTGGAGG+TGG | 0.555990 | 4:-31927945 | None:intergenic |
| ACTGCTAAAGCTTCTCGAGA+TGG | 0.596385 | 4:-31927726 | None:intergenic |
| AGCCTGAACCAGAAAATGCT+CGG | 0.612296 | 4:+31927820 | MsaT019592.1:CDS |
| AGGTCGAAAGCTTGAACAGG+AGG | 0.614849 | 4:-31927981 | None:intergenic |
| ATATTAGAATCTAGTAAACA+AGG | 0.648272 | 4:+31927780 | MsaT019592.1:CDS |
| CGAAAGCTTGAACAGGAGGC+TGG | 0.520977 | 4:-31927977 | None:intergenic |
| CTAGATTCTAATATATAATC+TGG | 0.344499 | 4:-31927771 | None:intergenic |
| CTCATTCATCTGGTGTATAA+GGG | 0.375685 | 4:-31928010 | None:intergenic |
| CTGGTGTATAAGGGAGATTG+AGG | 0.611261 | 4:-31928001 | None:intergenic |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 31927681 | 31928031 | 31927681 | ID=MsaG019592 |
| Chr4 | mRNA | 31927681 | 31928031 | 31927681 | ID=MsaT019592.1;Parent=MsaG019592 |
| Chr4 | exon | 31927681 | 31928031 | 31927681 | ID=MsaT019592.1.exon1;Parent=MsaT019592.1 |
| Chr4 | CDS | 31927681 | 31928031 | 31927681 | ID=cds.MsaT019592.1;Parent=MsaT019592.1 |
| Gene Sequence |
>MsaT019592.1 ATGTCAAAGCTAGAATTCTTATGCAGTGAATGTGACACAATGTTTCCATCTCGAGAAGCTTTAGCAGTGCATGTTATCGAAGAACATCATCCAGATTATATATTAGAATCTAGTAAACAAGGTCCACCACCAAATAACCAGCCTGAACCAGAAAATGCTCGGCCTCAACCACAAAATGTTGAACATCAACCACAAAATGCACCAGCTCCACCACAAAATGTTGAACATCAACCACAAAATGCTCCAGCTCCACCACAAAATGCACCACCTCCACATGTGCAACCATTTGACTTGAACCAGCCTCCTGTTCAAGCTTTCGACCTCAATCTCCCTTATACACCAGATGAATGA |
| Protein sequence |
>MsaT019592.1 MSKLEFLCSECDTMFPSREALAVHVIEEHHPDYILESSKQGPPPNNQPEPENARPQPQNVEHQPQNAPAPPQNVEHQPQNAPAPPQNAPPPHVQPFDLNQPPVQAFDLNLPYTPDE* |
