AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019593

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019593 MtrunA17_Chr4g0019481 80.342 117 22 1 1 116 1 117 1.28e-58 176
MsaG019593 MtrunA17_Chr4g0019471 71.756 131 22 2 1 116 1 131 5.84e-54 165
MsaG019593 MtrunA17_Chr4g0019551 77.778 117 25 1 1 116 1 117 9.92e-49 151
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCATTCATCTGGTGTATA+AGG 0.190805 4:-31952693 None:intergenic
TCGGGTTCAGGCCGGTTATT+TGG 0.231408 4:-31952492 None:intergenic
CTCGATTCTAATATAAAATC+TGG 0.312947 4:-31952453 None:intergenic
CTCATTCATCTGGTGTATAA+GGG 0.358870 4:-31952692 None:intergenic
AAGGTCCACCACCAAATAAC+CGG 0.447896 4:+31952481 MsaT019593.1:CDS
GGTTCAGGCCGGTTATTTGG+TGG 0.476028 4:-31952489 None:intergenic
TTGAGGTCGAAAGCTCGAAC+AGG 0.496472 4:-31952666 None:intergenic
GGAGGCTGGTTCAAGTCAAA+TGG 0.506887 4:-31952645 None:intergenic
TCAGGCCGGTTATTTGGTGG+TGG 0.516251 4:-31952486 None:intergenic
AAGTCAAATGGTTGCACATG+TGG 0.522527 4:-31952633 None:intergenic
CGAAAGCTCGAACAGGAGGC+TGG 0.528604 4:-31952659 None:intergenic
TTGTGTCACATTCACTGCAT+AGG 0.586276 4:-31952380 None:intergenic
ACTGCTAAAGCTTCTCGAGA+TGG 0.596385 4:-31952408 None:intergenic
CTGGTGTATAAGGGAGATTG+AGG 0.611261 4:-31952683 None:intergenic
ATATTAGAATCGAGTAAACA+AGG 0.624706 4:+31952462 MsaT019593.1:CDS
AGGTCGAAAGCTCGAACAGG+AGG 0.629084 4:-31952663 None:intergenic
TCAAATGGTTGCACATGTGG+AGG 0.671560 4:-31952630 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 31952363 31952713 31952363 ID=MsaG019593
Chr4 mRNA 31952363 31952713 31952363 ID=MsaT019593.1;Parent=MsaG019593
Chr4 exon 31952363 31952713 31952363 ID=MsaT019593.1.exon1;Parent=MsaT019593.1
Chr4 CDS 31952363 31952713 31952363 ID=cds.MsaT019593.1;Parent=MsaT019593.1
Gene Sequence

>MsaT019593.1

ATGTCAAAGCTAGAATTCCTATGCAGTGAATGTGACACAATGTTTCCATCTCGAGAAGCTTTAGCAGTGCATGTTATCGAAGAACATCATCCAGATTTTATATTAGAATCGAGTAAACAAGGTCCACCACCAAATAACCGGCCTGAACCCGAAAATGCTCAGCCTCAACCACAAAATGTTGAACATCAACCACAAAATGCACCAACTTCACCACAAAATGTTGAACATCAACCACAAAATGCACCAGCTCCACCACAAAATGCATCACCTCCACATGTGCAACCATTTGACTTGAACCAGCCTCCTGTTCGAGCTTTCGACCTCAATCTCCCTTATACACCAGATGAATGA
Protein sequence

>MsaT019593.1

MSKLEFLCSECDTMFPSREALAVHVIEEHHPDFILESSKQGPPPNNRPEPENAQPQPQNVEHQPQNAPTSPQNVEHQPQNAPAPPQNASPPHVQPFDLNQPPVRAFDLNLPYTPDE*