AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019597


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019597 MtrunA17_Chr4g0019481 73.504 117 17 2 1 103 1 117 9.67e-49 150
MsaG019597 MtrunA17_Chr4g0019551 76.923 117 13 2 1 103 1 117 2.80e-48 149
MsaG019597 MtrunA17_Chr4g0019471 65.649 131 17 2 1 103 1 131 5.65e-46 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCATTCATCTGGTGTATA+AGG 0.184471 4:-32048548 None:intergenic
TGTGGTTGAGGCTGATTATT+TGG 0.247122 4:-32048386 None:intergenic
CTCGATTCTAATATTAAATC+TGG 0.264896 4:-32048347 None:intergenic
CTCATTCATCTGGTGTATAA+GGG 0.356164 4:-32048547 None:intergenic
AGGTCGAAAGCGCGAACATT+AGG 0.356569 4:-32048518 None:intergenic
GGAGGTTGGTTTAAATCAAA+TGG 0.393156 4:-32048497 None:intergenic
CTGGTGTATAAGGGATATTA+AGG 0.496508 4:-32048538 None:intergenic
TGAGGCTGATTATTTGGTGG+TGG 0.505165 4:-32048380 None:intergenic
AAGCGCGAACATTAGGAGGT+TGG 0.511516 4:-32048511 None:intergenic
TCGAAAGCGCGAACATTAGG+AGG 0.542553 4:-32048515 None:intergenic
ACTGCTAAAGCTTGTGGAGA+TGG 0.545041 4:-32048302 None:intergenic
AAATCAAATGGTTGCACGCG+TGG 0.546490 4:-32048485 None:intergenic
AATGGTTGCACGCGTGGAGG+TGG 0.556418 4:-32048479 None:intergenic
ACATGCACTGCTAAAGCTTG+TGG 0.565788 4:-32048308 None:intergenic
GGTTGAGGCTGATTATTTGG+TGG 0.570406 4:-32048383 None:intergenic
TCAAATGGTTGCACGCGTGG+AGG 0.627906 4:-32048482 None:intergenic
GCATCAACCACAAAATGCAC+CGG 0.637007 4:+32048418 MsaT019597.1:CDS
ATATTAGAATCGAGTAAACA+AGG 0.646902 4:+32048356 MsaT019597.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 32048257 32048568 32048257 ID=MsaG019597
Chr4 mRNA 32048257 32048568 32048257 ID=MsaT019597.1;Parent=MsaG019597
Chr4 exon 32048257 32048568 32048257 ID=MsaT019597.1.exon1;Parent=MsaT019597.1
Chr4 CDS 32048257 32048568 32048257 ID=cds.MsaT019597.1;Parent=MsaT019597.1
Gene Sequence

>MsaT019597.1

ATGTCAAAGCTAGAATTCTTATGCAGTGAATGTGACACAATGTTTCCATCTCCACAAGCTTTAGCAGTGCATGTTATCGAAGAACATCATCCAGATTTAATATTAGAATCGAGTAAACAAGGTCCACCACCAAATAATCAGCCTCAACCACAAAATGATGAGCATCAACCACAAAATGCACCGGCTCTACCACAAAATGTTGAACATCAAGCACAAAATGCACCACCTCCACGCGTGCAACCATTTGATTTAAACCAACCTCCTAATGTTCGCGCTTTCGACCTTAATATCCCTTATACACCAGATGAATGA

Protein sequence

>MsaT019597.1

MSKLEFLCSECDTMFPSPQALAVHVIEEHHPDLILESSKQGPPPNNQPQPQNDEHQPQNAPALPQNVEHQAQNAPPPRVQPFDLNQPPNVRAFDLNIPYTPDE*